Lactococcus cremoris subsp. cremoris (DSM 20069, ATCC 19257, NCDO 607)

Name: Lactococcus cremoris subsp. cremoris (DSM 20069, ATCC 19257, NCDO 607)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14691

Type strain

Strain Designation HP

Culture col. no. DSM 20069 ATCC 19257 NCDO 607 NCIB 8662 CCUG 21953 12 more

NCBI tax ID(s) 1348655 1359 2816960

Special Collections DSMZ CCUG JCM CIP

History <- ATCC <- NCDO <- NIRD <- Whitehead, HP CIP 102301 <-- NCFB 607 <-- H. R. Whitehead strain HP. CIP <- 1986, NCFB, Streptococcus cremoris <- H.R. Whitehead: strain HP

Links

Lactococcus cremoris subsp. cremoris HP is a microaerophile, mesophilic, Gram-positive prokaryote of the family Streptococcaceae.

Gram-positive rod-shaped microaerophile mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 92452

LMG 6897,ATCC 19257,CCUG 21953,DSM 20069,IMET 10707,NCFB 607,NCIMB 8662,NBIMCC 8919,NCDO 607,NCIB 8662,CIP 102301,CECT 697,CCRC 12586,KCTC 3619,NBRC 100676,BCRC 12586,HAMBI 1588,NCIM 2402,CECT 7100,CNCTC 6910,VTT E-96275,JCM 16167

@ref 20215

Last LPSN update 2025-12-16 09:50:25

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Lactobacillales

Family Streptococcaceae

Genus Lactococcus

Species Lactococcus cremoris subsp. cremoris

Full scientific name Lactococcus cremoris subsp. cremoris (Orla-Jensen 1919) Li et al. 2021

BacDive ID	Other strains from **Lactococcus cremoris subsp. cremoris** (1)	Type strain
14692	L. cremoris subsp. cremoris 51732, DSM 20388

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Confidence
118783	positive	rod-shaped
125438			92.5
125438	positive		92.959

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8499	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
42090	MEDIUM 40- for Lactobacillus and Leuconostoc		Distilled water make up to (1000.000 ml);Man Rogosa Sharp agar (68.000 g)
118783	CIP Medium 72	Medium recipe at CIP
118783	CIP Medium 40	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8499	positive	growth	30	mesophilic
42090	positive	growth	30	mesophilic
47521	positive	growth	37	mesophilic
67770	positive	growth	30	mesophilic
118783	positive	growth	15-30
118783	negative	growth	10
118783	negative	growth	37
118783	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
47521	microaerophile
118783	facultative anaerobe
125439	obligate aerobe	96.7

Murein

@ref	Murein short key	Type
8499	A11.31	A4alpha L-Lys-D-Asp

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68381	40585 ChEBI	alpha-cyclodextrin	-	builds acid from	from API rID32STR
68381	29016 ChEBI	arginine	-	hydrolysis	from API rID32STR
68381	18333 ChEBI	D-arabitol	-	builds acid from	from API rID32STR
68381	16899 ChEBI	D-mannitol	-	builds acid from	from API rID32STR
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68381	16988 ChEBI	D-ribose	-	builds acid from	from API rID32STR
68379	16988 ChEBI	D-ribose	+	fermentation	from API Coryne
68381	16443 ChEBI	D-tagatose	-	builds acid from	from API rID32STR
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
68379	5291 ChEBI	gelatin	-	hydrolysis	from API Coryne
68381	28087 ChEBI	glycogen	-	builds acid from	from API rID32STR
68379	28087 ChEBI	glycogen	-	fermentation	from API Coryne
68381	30849 ChEBI	L-arabinose	-	builds acid from	from API rID32STR
68381	17716 ChEBI	lactose	+	builds acid from	from API rID32STR
68379	17716 ChEBI	lactose	+	fermentation	from API Coryne
68381	17306 ChEBI	maltose	-	builds acid from	from API rID32STR
68381	6731 ChEBI	melezitose	-	builds acid from	from API rID32STR
68381	28053 ChEBI	melibiose	-	builds acid from	from API rID32STR
68381	320055 ChEBI	methyl beta-D-glucopyranoside	-	builds acid from	from API rID32STR
68379	17632 ChEBI	nitrate	-	reduction	from API Coryne
118783	17632 ChEBI	nitrate	-	reduction
118783	17632 ChEBI	nitrate	+	respiration
118783	16301 ChEBI	nitrite	-	reduction
68381	27941 ChEBI	pullulan	-	builds acid from	from API rID32STR
68381	16634 ChEBI	raffinose	-	builds acid from	from API rID32STR
68381	30911 ChEBI	sorbitol	-	builds acid from	from API rID32STR
68381	17992 ChEBI	sucrose	-	builds acid from	from API rID32STR
68379	17992 ChEBI	sucrose	-	fermentation	from API Coryne
68381	27082 ChEBI	trehalose	-	builds acid from	from API rID32STR
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
68381	16199 ChEBI	urea	-	hydrolysis	from API rID32STR

Antibiotic resistance

@ref	Metabolite	Is sensitive	Is resistant
118783	0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68381	15688 ChEBI	acetoin		from API rID32STR

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68381	15688 ChEBI	acetoin	+	from API rID32STR

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68381	Alanyl-Phenylalanyl-Proline arylamidase	+		from API rID32STR
118783	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68379	alkaline phosphatase	-	3.1.3.1	from API Coryne
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68381	alpha-galactosidase	-	3.2.1.22	from API rID32STR
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68379	alpha-glucosidase	-	3.2.1.20	from API Coryne
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68381	arginine dihydrolase	-	3.5.3.6	from API rID32STR
68381	beta-galactosidase	-	3.2.1.23	from API rID32STR
68382	beta-galactosidase	-	3.2.1.23	from API zym
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68382	beta-glucosidase	-	3.2.1.21	from API zym
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68381	beta-glucuronidase	-	3.2.1.31	from API rID32STR
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68381	beta-mannosidase	-	3.2.1.25	from API rID32STR
118783	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68379	gelatinase	-		from API Coryne
68381	glycyl tryptophan arylamidase	-		from API rID32STR
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
118783	lysine decarboxylase	-	4.1.1.18
68381	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32STR
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68379	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API Coryne
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
118783	ornithine decarboxylase	-	4.1.1.17
118783	oxidase	-
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68381	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32STR
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68382	trypsin	-	3.4.21.4	from API zym
68381	urease	-	3.5.1.5	from API rID32STR
68379	urease	-	3.5.1.5	from API Coryne
68382	valine arylamidase	-		from API zym

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
47521	-	+	+	-	-	-	-	-	+	-	-	-	not determinedn.d.	+	-	-	not determinedn.d.	+	-	-	not determinedn.d.

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
118783	-	-	+	+	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-

API rID32STR

@ref	ADH (Arg)	beta GLU	beta GAR	beta GUR	alpha GAL	PAL	RIB	MAN	SOR	LAC	TRE	RAF	SAC	LARA	DARL	Acid from alpha-cyclodextrinCDEX	Acetoin production (Voges Proskauer test)VP	Alanyl-Phenylalanyl-Proline arylamidaseAPPA	beta GAL	Pyrrolidonyl arylamidasePyrA	N-Acetyl-glucosaminidasebeta NAG	Glycyl-tryptophan arylamidaseGTA	HIP	GLYG	PUL	MAL	MEL	MLZ	Acidification of methyl beta-D-glucopyranosideMbeta DG	TAG	beta MAN	URE
8499	-	+	+	-	-	-	-	-	-	+	-	-	-	-	-	-	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-
8499	-	+	+/-	-	-	+	-	-	-	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
8499	-	+	+	-	-	+/-	-	-	-	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-
8499	-	+/-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Food production	#Dairy product
#Engineered	#Food production	#Starter culture

Isolation

@ref	Country	Country ISO 3 Code	Continent	Sample type
42090	New zealand	NZL	Australia and Oceania
47521				Cheese starter culture
67770				Raw milk
118783	New Zealand	NZL	Australia and Oceania

Taxonmaps

69479

Global distribution of 16S sequence LC483568 (>99% sequence identity) for Lactococcus from Microbeatlas

Aquatic Samples	13841
Soil Samples	7135
Animal Samples	132033
Plant Samples	4677
Total Samples	157686

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
8499	yes, in single cases	yes, in single cases	1	Risk group (German classification) According to TRBA 466
118783			1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM159170v1 assembly for Lactococcus cremoris subsp. cremoris NBRC 100676	contig	GCA_001591705	1348655.3	2731957663	1348655	54.13
66792	HPAssemblyV1 assembly for Lactococcus cremoris subsp. cremoris HP	contig	GCA_000534815	1295827.3	2568526316	1295827	35.58
67770	ASM435451v1 assembly for Lactococcus cremoris ATCC 19257	scaffold	GCA_004354515	1359.227		1359	33.64
66792	ASM244176v1 assembly for Lactococcus cremoris ATCC 19257	scaffold	GCA_002441765	1359.117	2903255457	1359	33.38
67770	ASM162229v1 assembly for Lactococcus cremoris LMG6897	contig	GCA_001622295	1359.37	2740892534	1359	24.58
66792	ASM162221v1 assembly for Lactococcus cremoris HP	contig	GCA_001622215		2744054432	1359	15.6

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Lactococcus lactis subsp. lactis gene for 16S rRNA, partial sequence, strain: YIT 2008 (= ATCC 19435)	AB008215	1540	1360
20218	Streptococcus cremoris 16S ribosomal RNA	M58836	1496	1359
20218	Lactococcus lactis subsp. cremoris strain NIZO2418T 16S ribosomal RNA gene, partial sequence	EU091472	519	1359
20218	Lactococcus lactis subsp. cremoris gene for 16S rRNA, partial sequence, strain: NBRC 100676	AB681219	1466	1359
20218	Lactococcus lactis subsp. cremoris gene for 16S rRNA, partial sequence, strain: NCDO 607T	AB100802	1499	1359
67770	Lactococcus cremoris gene for 16S rRNA, partial sequence, strain: YIT 2007 (= ATCC 19257)	AB008214	1540	1359
67770	Lactococcus lactis subsp. cremoris gene for 16S ribosomal RNA, partial sequence	LC483568	1485	1359
124043	Lactococcus lactis subsp. cremoris strain DSM 20069 16S ribosomal RNA gene, partial sequence.	MK330519	1279	1359
124043	Lactococcus lactis subsp. cremoris strain DSM 20069 16S ribosomal RNA gene, partial sequence 16S-23S ribosomal RNA intergenic spacer, complete sequence and 23S ribosomal RNA gene, partial sequence.	MK330561	4317	1359

GC content

@ref	GC-content (mol%)	Method
8499	35.4
67770	34.8-35.6	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Lactococcus cremoris ATCC 19257
NCBI: GCA_002441765

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	70.10	no
125439	motility	BacteriaNetⓘ	no	67.70	no
125439	gram_stain	BacteriaNetⓘ	positive	71.00	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Lactococcus lactis subsp. cremoris NBRC 100676
PATRIC: 1348655.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	92.96	no
125438	anaerobic	anaerobicⓘ	no	89.07	no
125438	spore-forming	spore-formingⓘ	no	85.10	no
125438	aerobic	aerobicⓘ	no	96.37	no
125438	thermophilic	thermophileⓘ	no	97.36	yes
125438	flagellated	motile2+ⓘ	no	92.50	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Pathogenicity	Promysalin is a salicylate-containing antimicrobial with a cell-membrane-disrupting mechanism of action on Gram-positive bacteria.	Kaduskar RD, Scala GD, Al Jabri ZJH, Arioli S, Musso L, Oggioni MR, Dallavalle S, Mora D.	Sci Rep	10.1038/s41598-017-07567-0	2017
Enzymology	Development of an rRNA-targeted oligonucleotide probe specific for the genus Acinetobacter and its application for in situ monitoring in activated sludge.	Wagner M, Erhart R, Manz W, Amann R, Lemmer H, Wedi D, Schleifer KH.	Appl Environ Microbiol	10.1128/aem.60.3.792-800.1994	1994
Biotechnology	High-throughput ecological interaction mapping of dairy microorganisms.	Ndiaye A, Coulombe K, Fliss I, Filteau M.	Int J Food Microbiol	10.1016/j.ijfoodmicro.2024.110965	2025
	High-throughput characterization of the effect of sodium chloride and potassium chloride on 31 lactic acid bacteria and their co-cultures.	Ndiaye A, Fliss I, Filteau M.	Front Microbiol	10.3389/fmicb.2024.1328416	2024
	Comparison of Minimum Inhibitory Concentrations of Selected Antimicrobials for Non-Aureus Staphylococci, Enterococci, Lactococci, and Streptococci Isolated from Milk Samples of Cows with Clinical Mastitis.	Kolar QK, Goncalves JL, Erskine RJ, Ruegg PL.	Antibiotics (Basel)	10.3390/antibiotics13010091	2024
	Bacteriocin-Based Synergetic Consortia: a Promising Strategy to Enhance Antimicrobial Activity and Broaden the Spectrum of Inhibition.	Soltani S, Biron E, Ben Said L, Subirade M, Fliss I.	Microbiol Spectr	10.1128/spectrum.00406-21	2022
Genetics	Bacteriocin Antagonistic Potentials of Lactococcus cremoris and Lactococcus lactis Isolates from Different Habitats.	Tang T, Leisner JJ.	Probiotics Antimicrob Proteins	10.1007/s12602-024-10361-w	2025
Phylogeny	Quantification by real-time PCR of Lactococcus lactis subsp. cremoris in milk fermented by a mixed culture.	Grattepanche F, Lacroix C, Audet P, Lapointe G	Appl Microbiol Biotechnol	10.1007/s00253-004-1705-4	2004
Phylogeny	Elevation of Lactococcus lactis subsp. cremoris to the species level as Lactococcus cremoris sp. nov. and transfer of Lactococcus lactis subsp. tructae to Lactococcus cremoris as Lactococcus cremoris subsp. tructae comb. nov.	Li TT, Tian WL, Gu CT	Int J Syst Evol Microbiol	10.1099/ijsem.0.004727	2021

References

#8499	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20069
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#42090	; Curators of the CIP;
#47521	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 21953
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68379	Automatically annotated from API Coryne .
#68381	Automatically annotated from API rID32STR .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#118783	Collection of Institut Pasteur ; Curators of the CIP; CIP 102301
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Lactococcus cremoris subsp. cremoris (DSM 20069, ATCC 19257, NCDO 607)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Murein

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API coryne

API zym

API rID32STR

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_001591705

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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