Name: Gracilibacillus saliphilus DSM 19802
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1465

Type strain:

Species: Gracilibacillus saliphilus

Culture col. no.: DSM 19802, CCTCC AA 208015, YIM 91119

Strain history: <- S.-K. Tang and Y. Wang, YIM; YIM 91119

NCBI tax ID(s): 543890 (species)

SI-ID 396923

DSM 19802

Section

Gracilibacillus saliphilus DSM 19802 is an aerobe, spore-forming, mesophilic bacterium that forms circular colonies and has a creamy white pigmentation.

colony-forming
Gram-positive
motile
pigmented
rod-shaped
spore-forming
aerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Gracilibacillus*
	Species	**Gracilibacillus saliphilus**
	Full Scientific Name (LPSN)	Gracilibacillus saliphilus Tang et al. 2009

Information on morphological and physiological properties Morphology

[Ref.: #23047]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #23047]	Cell length	2.0-10.0 µm

[Ref.: #23047]	Cell width	0.7-0.9 µm

[Ref.: #23047]	Cell shape	rod-shaped

[Ref.: #23047]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.823

[Ref.: #23047]	Colony size	0.5-1.0 mm
[Ref.: #23047]	Colony color	creamy white
[Ref.: #23047]	Colony shape	circular
[Ref.: #23047]	Cultivation medium used	modified ISP5 medium

[Ref.: #23047]	Pigment production	yes
[Ref.: #23047]	Pigmentation color	creamy white

Information on culture and growth conditions Culture and growth conditions

[Ref.: #8326]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #8326]

Culture medium growth

[Ref.: #8326]

Culture medium link

Medium recipe at MediaDive

[Ref.: #8326]

Culture medium composition

expand / minimize

[Ref.: #23047]

Culture medium

modified ISP5 medium

[Ref.: #23047]

Culture medium growth

		Temperature
[Ref.: #8326]	growth	30 °C
[Ref.: #23047]	growth	4.0-45.0 °C
[Ref.: #23047]	optimum	28.0-37.0 °C

[Ref.: #8326]	Temperature range	mesophilic
[Ref.: #23047]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #23047]			optimum	7.0
[Ref.: #23047]			growth	6.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23047]

Oxygen tolerance

aerobe

[Ref.: #23047]	Spore description	terminal,spherical
[Ref.: #23047]	Type of spore	endospore
[Ref.: #23047]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #23047]

Halophily / tolerance level

moderately halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23047]		NaCl		growth	1.0-22.0	%(w/v)
[Ref.: #23047]		NaCl		optimum	10.0-15.0	%(w/v)

[Ref.: #23047]	Murein short key	A31
[Ref.: #23047]	Murein types	A1gamma m-Dpm-direct

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23047]	2-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #23047]	5-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #23047]	amygdalin ChEBI	+	builds acid from
[Ref.: #23047]	arbutin ChEBI	+	builds acid from
[Ref.: #23047]	casein	-	hydrolysis
[Ref.: #23047]	cellobiose ChEBI	+	builds acid from
[Ref.: #23047]	D-arabinose ChEBI	+	builds acid from
[Ref.: #23047]	D-arabitol ChEBI	-	builds acid from
[Ref.: #23047]	D-fructose ChEBI	+	builds acid from
[Ref.: #23047]	D-fucose ChEBI	-	builds acid from
[Ref.: #23047]	D-galactose ChEBI	+	builds acid from
[Ref.: #23047]	D-glucose ChEBI	+	builds acid from
[Ref.: #23047]	D-lyxose ChEBI	+	builds acid from
[Ref.: #23047]	D-mannitol ChEBI	+	builds acid from
[Ref.: #23047]	D-mannose ChEBI	+	builds acid from
[Ref.: #23047]	D-ribose ChEBI	+	builds acid from
[Ref.: #23047]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #23047]	D-tagatose ChEBI	+	builds acid from
[Ref.: #23047]	D-trehalose ChEBI	+	builds acid from
[Ref.: #23047]	D-xylose ChEBI	+	builds acid from
[Ref.: #23047]	erythritol ChEBI	-	builds acid from
[Ref.: #23047]	esculin ChEBI	+	builds acid from
[Ref.: #23047]	esculin ChEBI	+	hydrolysis
[Ref.: #23047]	galactitol ChEBI	-	builds acid from
[Ref.: #23047]	gentiobiose ChEBI	+	builds acid from
[Ref.: #23047]	gluconate ChEBI	+	builds acid from
[Ref.: #23047]	glucose ChEBI	+	fermentation
[Ref.: #23047]	glycerol ChEBI	+	builds acid from
[Ref.: #23047]	glycogen ChEBI	+	builds acid from
[Ref.: #23047]	inulin ChEBI	-	builds acid from
[Ref.: #23047]	L-arabinose ChEBI	+	builds acid from
[Ref.: #23047]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23047]	L-fucose ChEBI	+	builds acid from
[Ref.: #23047]	L-rhamnose ChEBI	+	builds acid from
[Ref.: #23047]	L-sorbose ChEBI	+	builds acid from
[Ref.: #23047]	L-xylose ChEBI	-	builds acid from
[Ref.: #23047]	lactose ChEBI	+	builds acid from
[Ref.: #23047]	maltose ChEBI	+	builds acid from
[Ref.: #23047]	melezitose ChEBI	-	builds acid from
[Ref.: #23047]	melibiose ChEBI	+	builds acid from
[Ref.: #23047]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #23047]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #23047]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from
[Ref.: #23047]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23047]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #23047]	nitrate ChEBI	+	reduction
[Ref.: #23047]	raffinose ChEBI	+	builds acid from
[Ref.: #23047]	ribitol ChEBI	-	builds acid from
[Ref.: #23047]	salicin ChEBI	+	builds acid from
[Ref.: #23047]	starch ChEBI	+	builds acid from
[Ref.: #23047]	starch ChEBI	+	hydrolysis
[Ref.: #23047]	sucrose ChEBI	+	builds acid from
[Ref.: #23047]	turanose ChEBI	+	builds acid from
[Ref.: #23047]	tween 40 ChEBI	-	hydrolysis
[Ref.: #23047]	tween 80 ChEBI	-	hydrolysis
[Ref.: #23047]	urea ChEBI	+	hydrolysis
[Ref.: #23047]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #23047]	amikacin ChEBI				yes	30	µg
[Ref.: #23047]	amoxicillin ChEBI	yes	10	µg
[Ref.: #23047]	ampicillin ChEBI				yes	10	µg
[Ref.: #23047]	chloramphenicol ChEBI	yes	30	µg
[Ref.: #23047]	ciprofloxacin ChEBI				yes	5	µg
[Ref.: #23047]	co-trimoxazole ChEBI	yes	1.25	µg
[Ref.: #23047]	erythromycin ChEBI				yes	15	µg
[Ref.: #23047]	gentamicin ChEBI				yes	10	µg
[Ref.: #23047]	lincomycin ChEBI	yes	2	µg
[Ref.: #23047]	neomycin ChEBI	yes	10	µg
[Ref.: #23047]	netilmycin ChEBI	yes	30	µg
[Ref.: #23047]	norfloxacin ChEBI	yes	10	µg
[Ref.: #23047]	novobiocin ChEBI				yes	30	µg
[Ref.: #23047]	penicillin ChEBI	yes	10	Unit
[Ref.: #23047]	rifampicin ChEBI	yes	5	µg
[Ref.: #23047]	streptomycin ChEBI				yes	10	µg
[Ref.: #23047]	tetracycline ChEBI	yes	30	µg
[Ref.: #23047]	tobramycin ChEBI				yes	10	µg
[Ref.: #23047]	vancomycin ChEBI				yes	30	µg
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23047]	acetoin ChEBI	yes
[Ref.: #23047]	hydrogen sulfide ChEBI	no
[Ref.: #23047]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #23047]		acetoin ChEBI	+
[Ref.: #23047]		glucose ChEBI		+

	Enzyme	Enzyme activity	EC number
[Ref.: #23047]	acid phosphatase	-	3.1.3.2
[Ref.: #23047]	alkaline phosphatase	+	3.1.3.1
[Ref.: #23047]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #23047]	alpha-fucosidase	-	3.2.1.51
[Ref.: #23047]	alpha-galactosidase	-	3.2.1.22
[Ref.: #23047]	alpha-glucosidase	-	3.2.1.20
[Ref.: #23047]	alpha-mannosidase	-	3.2.1.24
[Ref.: #23047]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23047]	beta-galactosidase	+	3.2.1.23
[Ref.: #23047]	beta-glucoronidase	-	3.2.1.31
[Ref.: #23047]	beta-glucosidase	+	3.2.1.21
[Ref.: #23047]	catalase	+	1.11.1.6
[Ref.: #23047]	cystine arylamidase	-	3.4.11.3
[Ref.: #23047]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23047]	esterase (C 4)	-
[Ref.: #23047]	esterase lipase (C 8)	+
[Ref.: #23047]	leucine arylamidase	-	3.4.11.1
[Ref.: #23047]	lipase (C 14)	-
[Ref.: #23047]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #23047]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #23047]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23047]	trypsin	-	3.4.21.4
[Ref.: #23047]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23047]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #8326]	Sample type/isolated from	saline soil
[Ref.: #8326]	Geographic location (country and/or sea, region)	Xinjiang province, Ebinur lake
[Ref.: #8326]	Country	China
[Ref.: #8326]	Country ISO 3 Code	CHN
[Ref.: #8326]	Continent	Asia

[Ref.: #23047]	Sample type/isolated from	soil sample
[Ref.: #23047]	Geographic location (country and/or sea, region)	Xinjiang province, Ebinur lake
[Ref.: #23047]	Country	China
[Ref.: #23047]	Country ISO 3 Code	CHN
[Ref.: #23047]	Continent	Asia
[Ref.: #23047]	Geographic location	45.0711°/82.5900°
[Ref.: #23047]	Enrichment culture	modified ISP5 medium
[Ref.: #23047]	Enrichment culture composition	containing 1 g L-asparagine, 10 g glycerol, 5 g yeast extract, 1 g K2HPO4 , 5 g KNO3 , 100 g NaCl and 15 g agar, per litre distilled water
* marker position based on {}

Isolation sources categories

#Condition	#Saline	-
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU784646 (>99% sequence identity) for Gracilibacillus from Microbeatlas

Aquatic Samples	31
Soil Samples	234
Animal Samples	107
Plant Samples	64
Total Samples	436

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #8326]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #8326]	Gracilibacillus saliphilus strain YIM 91119 16S ribosomal RNA gene, partial sequence	EU784646	1501	ENA	543890 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Gracilibacillus saliphilus YIM 91119	GCA_010994085	contig	GenBank	543890 tax ID	*
[Ref.: #66792]	Gracilibacillus saliphilus strain YIM 91119	543890.3	wgs	PATRIC	543890 tax ID	*

[Ref.: #8326]

GC-content

40.1 mol%

high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Gracilibacillus saliphilus strain YIM 91119 PATRIC: 543890.3
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Gracilibacillus saliphilus strain YIM 91119 PATRIC: 543890.3
[Ref.: #69480]	gram-positive	yes	87.766	yes
[Ref.: #69480]	anaerobic	no	99.403	no
[Ref.: #69480]	halophile	yes	89.419	no
[Ref.: #69480]	spore-forming	yes	93.186	yes
[Ref.: #69480]	glucose-util	yes	87.621	no
[Ref.: #69480]	thermophile	no	99.592	yes
[Ref.: #69480]	aerobic	yes	92.858	yes
[Ref.: #69480]	motile	yes	93.365	yes
[Ref.: #69480]	flagellated	yes	93.421	no
[Ref.: #69480]	glucose-ferment	no	72.258	yes

Availability in culture collections External links

[Ref.: #8326]

Culture collection no.

DSM 19802, CCTCC AA 208015, YIM 91119

[Ref.: #71111]

SI-ID 396923

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Gracilibacillus suaedae sp. nov., an indole acetic acid-producing endophyte isolated from a root of Suaeda salsa.	Huang XX, Xu L, Sun JQ	Int J Syst Evol Microbiol	10.1099/ijsem.0.005140	2021	*
Phylogeny	Gracilibacillus aidingensis sp. nov., a novel moderately halophilic bacterium isolated from Aiding salt lake.	Guan TW, Tian L, Li EY, Tang SK, Zhang XP	Arch Microbiol	10.1007/s00203-017-1399-5	2017	*
Phylogeny	Gracilibacillus bigeumensis sp. nov., a moderately halophilic bacterium from solar saltern soil.	Kim P, Lee JC, Park DJ, Shin KS, Kim JY, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.034264-0	2011	*
Phylogeny	Gracilibacillus thailandensis sp. nov., from fermented fish (pla-ra).	Chamroensaksri N, Tanasupawat S, Akaracharanya A, Visessanguan W, Kudo T, Itoh T	Int J Syst Evol Microbiol	10.1099/ijs.0.011981-0	2009	*
Phylogeny	Gracilibacillus saliphilus sp. nov., a moderately halophilic bacterium isolated from a salt lake.	Tang SK, Wang Y, Lou K, Mao PH, Jin X, Jiang CL, Xu LH, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.006569-0	2009	*

References

#8326

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19802

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23047

Shu-Kun Tang,Yun Wang,Kai Lou,Pei-Hong Mao,Xiang Jin,Cheng-Lin Jiang,Li-Hua Xu,Wen-Jun Li: Gracilibacillus saliphilus sp. nov., a moderately halophilic bacterium isolated from a salt lake. IJSEM 59: 1620 - 1624 2009 ( DOI 10.1099/ijs.0.006569-0 , PubMed 19542126 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71111

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID396923.1 )

* These data were automatically processed and therefore are not curated

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Gracilibacillus saliphilus strain YIM 91119

Gracilibacillus saliphilus strain YIM 91119

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