Gracilibacillus saliphilus (DSM 19802, CCTCC AA 208015, YIM 91119)

Name: Gracilibacillus saliphilus (DSM 19802, CCTCC AA 208015, YIM 91119)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1465

Type strain

Culture col. no. DSM 19802 CCTCC AA 208015 YIM 91119

NCBI tax ID(s) 543890

Special Collections DSMZ Literature strains

Links

Gracilibacillus saliphilus DSM 19802 is an aerobe, spore-forming, mesophilic prokaryote that forms circular colonies and has a creamy white pigmentation.

pigmented colony-forming rod-shaped motile Gram-positive spore-forming mesophilic aerobe 16S sequence genome sequence

Name and taxonomic classification

SI-ID 396923

DSM 19802

@ref 20215

Last LPSN update 2025-12-16 09:47:06

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Gracilibacillus

Species Gracilibacillus saliphilus

Full scientific name Gracilibacillus saliphilus Tang et al. 2009

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
23047	positive	2.0-10.0 µm	0.7-0.9 µm	rod-shaped

Colony morphology

@ref	Colony size	Colony color	Colony shape	Medium used
23047	0.5-1.0 mm	creamy white	circular	modified ISP5 medium

Pigmentation

@ref	Production	Color
23047		creamy white

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
8326	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)		Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514; with strain-specific modifications) Composition: NaCl 50.0 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
23047	modified ISP5 medium

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8326	positive	growth	30	mesophilic
23047	positive	growth	4.0-45.0
23047	positive	optimum	28.0-37.0	mesophilic

Culture pH

@ref	Ability	Type	PH
23047	positive	optimum	7.0
23047	positive	growth	6.0-8.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
23047	aerobe
125439	obligate aerobe	96.2

Spore formation

@ref	Spore description	Type of spore	Spore formation	Confidence
23047	terminal,spherical	endospore
125438				93.97

Halophily

@ref	Salt	Growth	Tested relation	Concentration	Halophily level
23047	NaCl	positive	growth	1.0-22.0 %(w/v)
23047	NaCl		optimum	10.0-15.0 %(w/v)
23047					moderately halophilic

Murein

@ref	Murein short key	Type
23047	A31	A1gamma m-Dpm-direct

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23047	16808 ChEBI	2-dehydro-D-gluconate	+	builds acid from
23047	17426 ChEBI	5-dehydro-D-gluconate	+	builds acid from
23047	27613 ChEBI	amygdalin	+	builds acid from
23047	18305 ChEBI	arbutin	+	builds acid from
23047		casein	-	hydrolysis
23047	17057 ChEBI	cellobiose	+	builds acid from
23047	17108 ChEBI	D-arabinose	+	builds acid from
23047	18333 ChEBI	D-arabitol	-	builds acid from
23047	15824 ChEBI	D-fructose	+	builds acid from
23047	28847 ChEBI	D-fucose	-	builds acid from
23047	12936 ChEBI	D-galactose	+	builds acid from
23047	17634 ChEBI	D-glucose	+	builds acid from
23047	62318 ChEBI	D-lyxose	+	builds acid from
23047	16899 ChEBI	D-mannitol	+	builds acid from
23047	16024 ChEBI	D-mannose	+	builds acid from
23047	16988 ChEBI	D-ribose	+	builds acid from
23047	17924 ChEBI	D-sorbitol	-	builds acid from
23047	16443 ChEBI	D-tagatose	+	builds acid from
23047	16551 ChEBI	D-trehalose	+	builds acid from
23047	65327 ChEBI	D-xylose	+	builds acid from
23047	17113 ChEBI	erythritol	-	builds acid from
23047	4853 ChEBI	esculin	+	builds acid from
23047	4853 ChEBI	esculin	+	hydrolysis
23047	16813 ChEBI	galactitol	-	builds acid from
23047	28066 ChEBI	gentiobiose	+	builds acid from
23047	24265 ChEBI	gluconate	+	builds acid from
23047	17234 ChEBI	glucose	+	fermentation
23047	17754 ChEBI	glycerol	+	builds acid from
23047	28087 ChEBI	glycogen	+	builds acid from
23047	15443 ChEBI	inulin	-	builds acid from
23047	30849 ChEBI	L-arabinose	+	builds acid from
23047	18403 ChEBI	L-arabitol	-	builds acid from
23047	18287 ChEBI	L-fucose	+	builds acid from
23047	62345 ChEBI	L-rhamnose	+	builds acid from
23047	17266 ChEBI	L-sorbose	+	builds acid from
23047	65328 ChEBI	L-xylose	-	builds acid from
23047	17716 ChEBI	lactose	+	builds acid from
23047	17306 ChEBI	maltose	+	builds acid from
23047	6731 ChEBI	melezitose	-	builds acid from
23047	28053 ChEBI	melibiose	+	builds acid from
23047	320061 ChEBI	methyl alpha-D-glucopyranoside	+	builds acid from
23047	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from
23047	74863 ChEBI	methyl beta-D-xylopyranoside	+	builds acid from
23047	17268 ChEBI	myo-inositol	-	builds acid from
23047	506227 ChEBI	N-acetylglucosamine	+	builds acid from
23047	17632 ChEBI	nitrate	+	reduction
23047	16634 ChEBI	raffinose	+	builds acid from
23047	15963 ChEBI	ribitol	-	builds acid from
23047	17814 ChEBI	salicin	+	builds acid from
23047	28017 ChEBI	starch	+	builds acid from
23047	28017 ChEBI	starch	+	hydrolysis
23047	17992 ChEBI	sucrose	+	builds acid from
23047	32528 ChEBI	turanose	+	builds acid from
23047	53423 ChEBI	tween 40	-	hydrolysis
23047	53426 ChEBI	tween 80	-	hydrolysis
23047	16199 ChEBI	urea	+	hydrolysis
23047	17151 ChEBI	xylitol	-	builds acid from

Antibiotic resistance

@ref	ChEBI	Metabolite	Resistance conc.	Sensitivity conc.
23047	2637	amikacin	30 µg
23047	2676	amoxicillin		10 µg
23047	28971	ampicillin	10 µg
23047	17698	chloramphenicol		30 µg
23047	100241	ciprofloxacin	5 µg
23047	3770	co-trimoxazole		1.25 µg
23047	48923	erythromycin	15 µg
23047	17833	gentamicin	10 µg
23047	6472	lincomycin		2 µg
23047	7507	neomycin		10 µg
23047	7528	netilmycin		30 µg
23047	100246	norfloxacin		10 µg
23047	28368	novobiocin	30 µg
23047	17334	penicillin		10 Unit
23047	28077	rifampicin		5 µg
23047	17076	streptomycin	10 µg
23047	27902	tetracycline		30 µg
23047	28864	tobramycin	10 µg
23047	28001	vancomycin	30 µg

Metabolite production

@ref	Chebi-ID	Metabolite
23047	15688 ChEBI	acetoin
23047	16136 ChEBI	hydrogen sulfide
23047	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
23047	15688 ChEBI	acetoin	+
23047	17234 ChEBI	glucose		+

Enzymes

@ref	Value	Activity	Ec
23047	acid phosphatase	-	3.1.3.2
23047	alkaline phosphatase	+	3.1.3.1
23047	alpha-chymotrypsin	-	3.4.21.1
23047	alpha-fucosidase	-	3.2.1.51
23047	alpha-galactosidase	-	3.2.1.22
23047	alpha-glucosidase	-	3.2.1.20
23047	alpha-mannosidase	-	3.2.1.24
23047	arginine dihydrolase	-	3.5.3.6
23047	beta-galactosidase	+	3.2.1.23
23047	beta-glucoronidase	-	3.2.1.31
23047	beta-glucosidase	+	3.2.1.21
23047	catalase	+	1.11.1.6
23047	cystine arylamidase	-	3.4.11.3
23047	cytochrome oxidase	+	1.9.3.1
23047	esterase (C 4)	-
23047	esterase lipase (C 8)	+
23047	leucine arylamidase	-	3.4.11.1
23047	lipase (C 14)	-
23047	N-acetyl-beta-glucosaminidase	-	3.2.1.52
23047	naphthol-AS-BI-phosphohydrolase	+
23047	ornithine decarboxylase	-	4.1.1.17
23047	trypsin	-	3.4.21.4
23047	tryptophan deaminase	-	4.1.99.1
23047	valine arylamidase	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Condition	#Saline	-
#Environmental	#Terrestrial	#Soil

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture composition
8326	saline soil	Xinjiang province, Ebinur lake	China	CHN	Asia
23047	soil sample	Xinjiang province, Ebinur lake	China	CHN	Asia	45.0711	82.59 45.0711/82.59	modified ISP5 medium	containing 1 g L-asparagine, 10 g glycerol, 5 g yeast extract, 1 g K2HPO4 , 5 g KNO3 , 100 g NaCl and 15 g agar, per litre distilled water

Taxonmaps

69479

Global distribution of 16S sequence EU784646 (>99% sequence identity) for Gracilibacillus from Microbeatlas

Aquatic Samples	31
Soil Samples	234
Animal Samples	107
Plant Samples	64
Total Samples	436

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8326	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1099408v1 assembly for Gracilibacillus saliphilus YIM 91119	contig	GCA_010994085	543890.3	8066007470	543890	59.69

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
8326	Gracilibacillus saliphilus strain YIM 91119 16S ribosomal RNA gene, partial sequence	EU784646	1501		543890

GC content

@ref	GC-content (mol%)	Method
8326	40.1	high performance liquid chromatography (HPLC)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Gracilibacillus saliphilus YIM 91119
NCBI: GCA_010994085

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	83.40	no
125439	motility	BacteriaNetⓘ	yes	77.00	no
125439	gram_stain	BacteriaNetⓘ	variable	79.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	96.20	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Gracilibacillus saliphilus strain YIM 91119
PATRIC: 543890.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	76.68	yes
125438	anaerobic	anaerobicⓘ	no	96.54	yes
125438	spore-forming	spore-formingⓘ	yes	93.97	yes
125438	aerobic	aerobicⓘ	yes	72.32	yes
125438	thermophilic	thermophileⓘ	no	93.80	no
125438	flagellated	motile2+ⓘ	yes	85.65	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Isolation of a novel halothermophilic strain of the genus Gracilibacillus from Howz-e Sultan hypersaline lake in Iran.	Mahmoudnia F.	Iran J Microbiol	10.18502/ijm.v13i3.6403	2021
Enzymology	[Bacterial biodiversity in Dongcai, a traditional pickled mustard product in Sichuan Province, China].	Dong L, Pu B, Ao X, Zhang X, Zheng Y, Li X.	Wei Sheng Wu Xue Bao		2012
Enzymology	Isolation and diversity of sediment bacteria in the hypersaline aiding lake, China.	Guan TW, Lin YJ, Ou MY, Chen KB.	PLoS One	10.1371/journal.pone.0236006	2020
	Gracilibacillus pellucidus sp. nov., a moderately halophilic bacterium isolated from saline soil in Xinjiang province, China.	Zhang Z, Wang H, Xia M, Li W, Zhang P, Wang Y, Liu L, Li P, Zhuang Y, Tan F.	Antonie Van Leeuwenhoek	10.1007/s10482-025-02084-x	2025
Phylogeny	Gracilibacillus suaedae sp. nov., an indole acetic acid-producing endophyte isolated from a root of Suaeda salsa.	Huang XX, Xu L, Sun JQ	Int J Syst Evol Microbiol	10.1099/ijsem.0.005140	2021
Phylogeny	Gracilibacillus aidingensis sp. nov., a novel moderately halophilic bacterium isolated from Aiding salt lake.	Guan TW, Tian L, Li EY, Tang SK, Zhang XP	Arch Microbiol	10.1007/s00203-017-1399-5	2017
Phylogeny	Gracilibacillus bigeumensis sp. nov., a moderately halophilic bacterium from solar saltern soil.	Kim P, Lee JC, Park DJ, Shin KS, Kim JY, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.034264-0	2011
Phylogeny	Gracilibacillus thailandensis sp. nov., from fermented fish (pla-ra).	Chamroensaksri N, Tanasupawat S, Akaracharanya A, Visessanguan W, Kudo T, Itoh T	Int J Syst Evol Microbiol	10.1099/ijs.0.011981-0	2009
Phylogeny	Gracilibacillus saliphilus sp. nov., a moderately halophilic bacterium isolated from a salt lake.	Tang SK, Wang Y, Lou K, Mao PH, Jin X, Jiang CL, Xu LH, Li WJ	Int J Syst Evol Microbiol	10.1099/ijs.0.006569-0	2009

References

#8326	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19802
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23047	Shu-Kun Tang,Yun Wang,Kai Lou,Pei-Hong Mao,Xiang Jin,Cheng-Lin Jiang,Li-Hua Xu,Wen-Jun Li: Gracilibacillus saliphilus sp. nov., a moderately halophilic bacterium isolated from a salt lake. IJSEM 59: 1620 - 1624 2009 ( DOI 10.1099/ijs.0.006569-0 , PubMed 19542126 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Gracilibacillus saliphilus (DSM 19802, CCTCC AA 208015, YIM 91119)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Murein

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_010994085

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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