Name: Gracilibacillus lacisalsi BH312
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1464

Type strain:

Species: Gracilibacillus lacisalsi

Strain Designation: BH312

Culture col. no.: DSM 19029, KCTC 13129

Strain history: <- CJ Kim, KRIBB

NCBI tax ID(s): 1121929 (strain), 393087 (species)

SI-ID 399390

DSM 19029

Section

Gracilibacillus lacisalsi BH312 is an obligate aerobe, halophilic, spore-forming bacterium that forms circular colonies and was isolated from sediment near the lake bank.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
halophilic
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Gracilibacillus*
	Species	**Gracilibacillus lacisalsi**
	Full Scientific Name (LPSN)	Gracilibacillus lacisalsi Jeon et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #23046]	Gram stain	positive
[Ref.: #67771]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #23046]	Cell length	1.0-2.2 µm

[Ref.: #23046]	Cell width	0.2-1.0 µm

[Ref.: #23046]	Cell shape	rod-shaped
[Ref.: #67771]	Cell shape	rod-shaped

[Ref.: #23046]	Motility	yes
[Ref.: #67771]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %97.055

[Ref.: #23046]	Flagellum arrangement	amphitrichous
[Ref.: #67771]	Flagellum arrangement	amphitrichous

[Ref.: #23046]	Colony color	cream
[Ref.: #23046]	Colony shape	circular
[Ref.: #23046]	Incubation period	2 days
[Ref.: #23046]	Cultivation medium used	MA with the addition of 3 % (w/v) NaCl

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7849]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

[Ref.: #23046]

Culture medium

MA with the addition of 3 % (w/v) NaCl

[Ref.: #23046]

Culture medium growth

		Temperature
[Ref.: #7849]	growth	30 °C
[Ref.: #23046]	growth	15.0-50.0 °C
[Ref.: #23046]	optimum	40.0 °C
[Ref.: #67771]	growth	40 °C

[Ref.: #7849]	Temperature range	mesophilic
[Ref.: #23046]	Temperature range	thermophilic
[Ref.: #67771]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #23046]			optimum	7.5-8.0
[Ref.: #23046]			growth	5.5-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23046]	Oxygen tolerance	obligate aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #23046]	Spore description	spherical,terminal in swollen sporangium
[Ref.: #23046]	Type of spore	endospore
[Ref.: #23046]	Ability of spore formation	yes
[Ref.: #67771]	Type of spore	spore
[Ref.: #67771]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #23046]

Halophily / tolerance level

halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23046]		NaCl		optimum	5.0-7.0	%(w/v)
[Ref.: #23046]		NaCl		growth	1.0-18.0	%(w/v)

[Ref.: #67771]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23046]	alpha-lactose ChEBI	+	builds acid from
[Ref.: #23046]	casein	-	hydrolysis
[Ref.: #23046]	D-fructose ChEBI	+	builds acid from
[Ref.: #23046]	D-glucose ChEBI	+	builds acid from
[Ref.: #23046]	D-mannitol ChEBI	+	builds acid from
[Ref.: #23046]	D-mannose ChEBI	+	builds acid from
[Ref.: #23046]	esculin ChEBI	+	hydrolysis
[Ref.: #23046]	galactose ChEBI	+	builds acid from
[Ref.: #23046]	glycerol ChEBI	+	builds acid from
[Ref.: #23046]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #23046]	L-arabinose ChEBI	+	builds acid from
[Ref.: #23046]	maltose ChEBI	+	builds acid from
[Ref.: #23046]	melibiose ChEBI	+	builds acid from
[Ref.: #23046]	myo-inositol ChEBI	-	builds acid from
[Ref.: #23046]	nitrate ChEBI	+	reduction
[Ref.: #23046]	raffinose ChEBI	+	builds acid from
[Ref.: #23046]	starch ChEBI	-	hydrolysis
[Ref.: #23046]	sucrose ChEBI	+	builds acid from
[Ref.: #23046]	tween 80 ChEBI	-	hydrolysis
[Ref.: #23046]	urea ChEBI	-	hydrolysis
[Ref.: #23046]	xanthine ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #23046]	acid phosphatase	-	3.1.3.2
[Ref.: #23046]	alkaline phosphatase	-	3.1.3.1
[Ref.: #23046]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #23046]	alpha-fucosidase	-	3.2.1.51
[Ref.: #23046]	alpha-galactosidase	-	3.2.1.22
[Ref.: #23046]	alpha-glucosidase	+	3.2.1.20
[Ref.: #23046]	alpha-mannosidase	-	3.2.1.24
[Ref.: #23046]	beta-galactosidase	+	3.2.1.23
[Ref.: #23046]	beta-glucosidase	+	3.2.1.21
[Ref.: #23046]	beta-glucuronidase	-	3.2.1.31
[Ref.: #23046]	catalase	+	1.11.1.6
[Ref.: #23046]	cystine arylamidase	-	3.4.11.3
[Ref.: #23046]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23046]	esterase (C 4)	+
[Ref.: #23046]	esterase lipase (C 8)	+
[Ref.: #23046]	leucine arylamidase	+	3.4.11.1
[Ref.: #23046]	lipase (C 14)	-
[Ref.: #23046]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #23046]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #23046]	trypsin	-	3.4.21.4
[Ref.: #23046]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7849]	Sample type/isolated from	sediment near the lake bank
[Ref.: #7849]	Geographic location (country and/or sea, region)	Ai-Ding Lake, Xin-Jiang Province, China
[Ref.: #7849]	Country	China
[Ref.: #7849]	Country ISO 3 Code	CHN
[Ref.: #7849]	Continent	Asia

[Ref.: #23046]	Sample type/isolated from	salt lake
[Ref.: #23046]	Enrichment culture	saline solution (0.9 %, w/v), spread on marine agar 2216 (MA; Difco) with the addition of 10 % (w/v) NaCl [ final concentration of 11.94 % NaCl (w/v) ]
[Ref.: #23046]	Enrichment culture duration	2 days
[Ref.: #23046]	Enrichment culture temperature	35.0 ̊C

[Ref.: #67771]	Sample type/isolated from	From soil
[Ref.: #67771]	Geographic location (country and/or sea, region)	Xinjiang
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Condition	#Saline	-
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Sediment

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7849]	Gracilibacillus sp. BH312 16S ribosomal RNA gene, partial sequence	DQ664540	1476	ENA	1121929 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Gracilibacillus lacisalsi DSM 19029	GCA_000377765	scaffold	GenBank	1121929 tax ID	*
[Ref.: #66792]	Gracilibacillus lacisalsi DSM 19029	1121929.3	wgs	PATRIC	1121929 tax ID	*
[Ref.: #66792]	Gracilibacillus lacisalsi DSM 19029	2519103189	draft	JGI IMG/M	1121929 tax ID	*

[Ref.: #7849]	GC-content	39.0 mol%	high performance liquid chromatography (HPLC)
[Ref.: #23046]	GC-content	38.8-39.0 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	38.84 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Gracilibacillus lacisalsi DSM 19029 DSM 19029 NCBI: GCA_000377765.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Gracilibacillus lacisalsi DSM 19029 DSM 19029 NCBI: GCA_000377765.1
[Ref.: #69480]	motile	yes	92.61	no
[Ref.: #69480]	flagellated	yes	90.762	no
[Ref.: #69480]	gram-positive	yes	92.739	yes
[Ref.: #69480]	anaerobic	no	99.349	yes
[Ref.: #69480]	aerobic	yes	92.024	yes
[Ref.: #69480]	halophile	yes	93.094	no
[Ref.: #69480]	spore-forming	yes	90.367	no
[Ref.: #69480]	glucose-util	yes	89.26	no
[Ref.: #69480]	thermophile	no	99.125	no
[Ref.: #69480]	glucose-ferment	no	78.06	no

Availability in culture collections External links

[Ref.: #7849]

Culture collection no.

DSM 19029, KCTC 13129

[Ref.: #71110]

SI-ID 399390

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Gracilibacillus suaedae sp. nov., an indole acetic acid-producing endophyte isolated from a root of Suaeda salsa.	Huang XX, Xu L, Sun JQ	Int J Syst Evol Microbiol	10.1099/ijsem.0.005140	2021	*
Phylogeny	Gracilibacillus lacisalsi sp. nov., a halophilic Gram-positive bacterium from a salt lake in China.	Jeon CO, Lim JM, Jang HH, Park DJ, Xu LH, Jiang CL, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.65369-0	2008	*

References

#7849

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19029

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#23046

Che Ok Jeon,Jee-Min Lim,Ho Hee Jang,Dong-Jin Park,Li-Hua Xu,Cheng-Lin Jiang,Chang-Jin Kim: Gracilibacillus lacisalsi sp. nov., a halophilic Gram-positive bacterium from a salt lake in China. IJSEM 58: 2282 - 2286 2008 ( DOI 10.1099/ijs.0.65369-0 , PubMed 18842841 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71110

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID399390.1 )

* These data were automatically processed and therefore are not curated

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Gracilibacillus lacisalsi DSM 19029 DSM 19029

Gracilibacillus lacisalsi DSM 19029 DSM 19029

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