Name: Gracilibacillus quinghaiensis DSM 17858
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1462

Type strain:

Species: Gracilibacillus quinghaiensis

Culture col. no.: DSM 17858, CGMCC 1.6304, YIM C229, CIP 108074

Strain history: CIP <- 2003, W.J. Li, Yunnan Univ., Kunming, China: strain YIM KN4, 2008 <- Xiao-Long Cui: strain YIM C229

NCBI tax ID(s): 470774 (species)

Section

Gracilibacillus quinghaiensis DSM 17858 is an obligate aerobe, spore-forming, mesophilic bacterium that was isolated from sediment.

Gram-positive
motile
rod-shaped
spore-forming
mesophilic
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Gracilibacillus*
	Species	"Gracilibacillus quinghaiensis"
	Full Scientific Name (LPSN)	Gracilibacillus quinghaiensis Chen et al. 2008

Information on morphological and physiological properties Morphology

[Ref.: #122096]	Gram stain	positive

[Ref.: #122096]	Cell shape	rod-shaped

[Ref.: #122096]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #7144]

Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

[Ref.: #7144]

Culture medium growth

[Ref.: #7144]

Culture medium link

Medium recipe at MediaDive

[Ref.: #7144]

Culture medium composition

expand / minimize

[Ref.: #33888]

Culture medium

Marine agar (MA)

[Ref.: #33888]

Culture medium growth

[Ref.: #33888]

Culture medium composition

expand / minimize

[Ref.: #122096]

Culture medium

CIP Medium 13

[Ref.: #122096]

Culture medium growth

[Ref.: #122096]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #7144]		growth	30 °C
[Ref.: #33888]		growth	30 °C
[Ref.: #122096]		growth	15-37 °C
[Ref.: #122096]	no	growth	5 °C
[Ref.: #122096]	no	growth	41 °C

[Ref.: #7144]	Temperature range	mesophilic
[Ref.: #33888]	Temperature range	mesophilic
[Ref.: #122096]	Temperature range	psychrophilic
[Ref.: #122096]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #122096]

Oxygen tolerance

obligate aerobe

[Ref.: #122096]

Ability of spore formation

yes

	Salt	Tested relation		Salt conc.
[Ref.: #122096]	NaCl	no	growth	0	%
[Ref.: #122096]	NaCl	no	growth	2	%
[Ref.: #122096]	NaCl	no	growth	4	%
[Ref.: #122096]	NaCl	no	growth	6	%
[Ref.: #122096]	NaCl	no	growth	8	%
[Ref.: #122096]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68368]	arginine ChEBI	-	hydrolysis	* from API 20E
[Ref.: #122096]	cellobiose ChEBI	-	degradation
[Ref.: #122096]	citrate ChEBI	-	carbon source
[Ref.: #68368]	citrate ChEBI	-	assimilation	* from API 20E
[Ref.: #122096]	D-arabinose ChEBI	-	degradation
[Ref.: #122096]	D-fructose ChEBI	-	degradation
[Ref.: #122096]	D-glucose ChEBI	-	degradation
[Ref.: #68368]	D-glucose ChEBI	-	fermentation	* from API 20E
[Ref.: #122096]	D-mannose ChEBI	-	degradation
[Ref.: #122096]	D-xylose ChEBI	-	degradation
[Ref.: #122096]	esculin ChEBI	+	hydrolysis
[Ref.: #68368]	gelatin ChEBI	-	hydrolysis	* from API 20E
[Ref.: #122096]	glucose ChEBI	-	fermentation
[Ref.: #122096]	lactose ChEBI	-	degradation
[Ref.: #122096]	lactose ChEBI	-	fermentation
[Ref.: #68368]	lysine ChEBI	-	degradation	* from API 20E
[Ref.: #122096]	maltose ChEBI	-	degradation
[Ref.: #122096]	nitrate ChEBI	-	reduction
[Ref.: #122096]	nitrate ChEBI	-	respiration
[Ref.: #68368]	nitrate ChEBI	-	reduction	* from API 20E
[Ref.: #122096]	nitrite ChEBI	-	reduction
[Ref.: #68368]	ornithine ChEBI	-	degradation	* from API 20E
[Ref.: #122096]	salicin ChEBI	-	degradation
[Ref.: #122096]	sodium thiosulfate ChEBI	-	builds gas from
[Ref.: #122096]	sucrose ChEBI	-	degradation
[Ref.: #68368]	tryptophan ChEBI	-	energy source	* from API 20E
[Ref.: #68368]	urea ChEBI	-	hydrolysis	* from API 20E
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #122096]	indole ChEBI	no
[Ref.: #68368]	acetoin ChEBI	no	* from API 20E
[Ref.: #68368]	dinitrogen ChEBI	no	* from API 20E
[Ref.: #68368]	hydrogen sulfide ChEBI	no	* from API 20E
[Ref.: #68368]	indole ChEBI	no	* from API 20E
[Ref.: #68368]	nitrite ChEBI	no	* from API 20E

	Metabolite	Voges-Proskauer-test	Methylred-test	Indole test
[Ref.: #122096]	acetoin ChEBI	-
[Ref.: #122096]	glucose ChEBI		-
[Ref.: #68368]	acetoin ChEBI	-			* from API 20E
[Ref.: #68368]	indole ChEBI			-	* from API 20E

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	-	3.1.3.2	* from API zym
[Ref.: #122096]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #122096]	amylase	-
[Ref.: #68368]	arginine dihydrolase	-	3.5.3.6	* from API 20E
[Ref.: #122096]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	+	3.2.1.23	* from API zym
[Ref.: #68368]	beta-galactosidase	-	3.2.1.23	* from API 20E
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #122096]	caseinase	+	3.4.21.50
[Ref.: #122096]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #122096]	gamma-glutamyltransferase	+	2.3.2.2
[Ref.: #122096]	gelatinase	-
[Ref.: #68368]	gelatinase	-		* from API 20E
[Ref.: #122096]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #122096]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #122096]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68368]	lysine decarboxylase	-	4.1.1.18	* from API 20E
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	-		* from API zym
[Ref.: #122096]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68368]	ornithine decarboxylase	-	4.1.1.17	* from API 20E
[Ref.: #122096]	oxidase	+
[Ref.: #122096]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #122096]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #122096]	tryptophan deaminase	-
[Ref.: #68368]	tryptophan deaminase	-	4.1.99.1	* from API 20E
[Ref.: #122096]	tween esterase	+
[Ref.: #122096]	urease	-	3.5.1.5
[Ref.: #68368]	urease	-	3.5.1.5	* from API 20E
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® 20E:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	beta-GalactosidaseONPG	Arginine dihydrolaseADH (Arg)	Lysine decarboxylaseLDC (Lys)	Ornithine decarboxylaseODC	Citrate utilizationCIT	H2S productionH2S	Urease/urea hydrolysisURE	Tryptophan deaminaseTDA (Trp)	Indole productionIND	Acetoin production (Voges Proskauer test)VP	Gelatinase/gelatin hydrolysisGEL	Fermentation/oxidation of D-glucoseGLU	Fermentation/oxidation of D-mannitolMAN	Fermentation/oxidation of inositolINO	Fermentation/oxidation of sorbitolSor	Fermentation/oxidation of L-rhamnoseRHA	Fermentation/oxidation of sucroseSAC	Fermentation/oxidation of melibioseMEL	Fermentation/oxidation of amygdalinAMY	Fermentation/oxidation of L-arabinoseARA	Cytochrome oxidaseOX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	Oxidative catabolisation of D-glucoseOF-O	Fermentation of D-glucoseOF-F
[Ref.: #122096]	15656	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #122096]	2569	not determinedn.d.	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #122096]	10863	-	+	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #7144]	Sample type/isolated from	sediment
[Ref.: #7144]	Geographic location (country and/or sea, region)	Xiaochaidamu salt lake, Qaidam Basin, Qinghai Province
[Ref.: #7144]	Country	China
[Ref.: #7144]	Country ISO 3 Code	CHN
[Ref.: #7144]	Continent	Asia

[Ref.: #122096]	Sample type/isolated from	Environment, Sediment
[Ref.: #122096]	Geographic location (country and/or sea, region)	Qinghai
[Ref.: #122096]	Country	China
[Ref.: #122096]	Country ISO 3 Code	CHN
[Ref.: #122096]	Continent	Asia
[Ref.: #122096]	Isolation date	2002
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Sediment
#Environmental	#Terrestrial	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence EU135723 (>99% sequence identity) for Gracilibacillus from Microbeatlas

Aquatic Samples	111
Soil Samples	251
Animal Samples	166
Plant Samples	52
Total Samples	580

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #122096]

Biosafety level

Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #7144]	Gracilibacillus quinghaiensis strain YIM-C229 16S ribosomal RNA gene, partial sequence	EU135723	1517	ENA	470774 tax ID

[Ref.: #7144]

GC-content

40.9 mol%

Availability in culture collections External links

[Ref.: #7144]

Culture collection no.

DSM 17858, CGMCC 1.6304, YIM C229, CIP 108074

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Gracilibacillus quinghaiensis sp. nov., isolated from salt-lake sediment in the Qaidam Basin, north-west China.	Chen YG, Cui XL, Zhang YQ, Li WJ, Wang YX, Xu LH, Peng Q, Wen ML, Jiang CL	Syst Appl Microbiol	10.1016/j.syapm.2008.05.001	2008	*
Phylogeny	Gracilibacillus bigeumensis sp. nov., a moderately halophilic bacterium from solar saltern soil.	Kim P, Lee JC, Park DJ, Shin KS, Kim JY, Kim CJ	Int J Syst Evol Microbiol	10.1099/ijs.0.034264-0	2011	*

References

#7144

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17858

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#33888 ; Curators of the CIP;

#68368

Automatically annotated from API 20E .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#122096 Collection of Institut Pasteur ; Curators of the CIP; CIP 108074
* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Gracilibacillus quinghaiensis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF