Staphylococcus muscae (DSM 7068, ATCC 49910, CCM 4175)

Name: Staphylococcus muscae (DSM 7068, ATCC 49910, CCM 4175)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14605

Type strain

Strain Designation MB4

Culture col. no. DSM 7068 ATCC 49910 CCM 4175 CCUG 36972 MB4 1 more

NCBI tax ID(s) 1294

Special Collections DSMZ CCUG CIP

History <- CCM <- V. Hajek, MB4 1993, V. Hajek, Inst. Med. Microbiol., Olomouc, Czechoslovakia: strain MB4 CIP <- 1993, V. Hajek, Palacky Univ., Olomouc, Czech Republic: strain MB4

Links

Staphylococcus muscae MB4 is an aerobe, Gram-positive, coccus-shaped bacterium that was isolated from fly, body surface.

Gram-positive coccus-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 42748

NCTC 13833,ATCC 49910,CCM 4175,CCUG 36972,CIP 103641,DSM 7068,KCTC 3576

@ref 20215

Last LPSN update 2026-02-09 11:21:27

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Staphylococcaceae

Genus Staphylococcus

Species Staphylococcus muscae

Full scientific name Staphylococcus muscae Hájek et al. 1992

BacDive ID	Other strains from **Staphylococcus muscae** (1)	Type strain
149161	S. muscae CCUG 36973, CCM 4177

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
121204	positive	coccus-shaped

Colony morphology

@ref	Type of hemolysis	Hemolysis ability
2969	beta	1
121204		1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
2969	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
2969	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
40749	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
121204	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
2969	positive	growth	37
40749	positive	growth	37
53517	positive	growth	37
121204	positive	growth	37
121204	negative	growth	15
121204	negative	growth	45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
53517	aerobe
121204	facultative anaerobe
125439	microaerophile	98.7

Murein

@ref	Murein short key	Type
2969	A11.02	A3alpha L-Lys-Gly_5-6

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68375	22599 ChEBI	arabinose	-	fermentation	from API ID32STA
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68375	29016 ChEBI	arginine	-	hydrolysis	from API ID32STA
68375	17057 ChEBI	cellobiose	-	fermentation	from API ID32STA
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68375	16024 ChEBI	D-mannose	-	fermentation	from API ID32STA
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	-	builds acid from	from API 50CH acid
68375	16988 ChEBI	D-ribose	-	fermentation	from API ID32STA
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68375	4853 ChEBI	esculin	-	hydrolysis	from API ID32STA
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	+	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68375	17306 ChEBI	maltose	-	fermentation	from API ID32STA
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
68375	17632 ChEBI	nitrate	+	reduction	from API ID32STA
121204	17632 ChEBI	nitrate	+	reduction
121204	16301 ChEBI	nitrite	-	reduction
68375	18257 ChEBI	ornithine	-	degradation	from API ID32STA
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68375	16634 ChEBI	raffinose	-	fermentation	from API ID32STA
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
121204	35020 ChEBI	tributyrin	-	hydrolysis
68375	32528 ChEBI	turanose	-	fermentation	from API ID32STA
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	ChEBI	Metabolite	Is sensitive	Sensitivity conc.	Is resistant
121204		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)
68375	28368	novobiocin		1.8 µg		from API ID32STA

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68375	15688 ChEBI	acetoin		from API ID32STA
121204	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test
68375	15688 ChEBI	acetoin	-	from API ID32STA
121204	15688 ChEBI	acetoin	-

Enzymes

@ref	Value	Activity	Ec
121204	alcohol dehydrogenase	-	1.1.1.1
68375	arginine dihydrolase	-	3.5.3.6	from API ID32STA
121204	beta-galactosidase	-	3.2.1.23
68375	beta-galactosidase	-	3.2.1.23	from API ID32STA
68375	beta-glucosidase	-	3.2.1.21	from API ID32STA
68375	beta-glucuronidase	+	3.2.1.31	from API ID32STA
121204	catalase	+	1.11.1.6
121204	coagulase	-
121204	DNase	+/-
121204	gelatinase	-
68375	L-arginine arylamidase	-		from API ID32STA
121204	lecithinase	+
121204	lysine decarboxylase	-	4.1.1.18
121204	ornithine decarboxylase	-	4.1.1.17
68375	ornithine decarboxylase	-	4.1.1.17	from API ID32STA
121204	oxidase	-
68375	pyrrolidonyl arylamidase	-	3.4.19.3	from API ID32STA
121204	tween esterase	+
121204	urease	-	3.5.1.5

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
121204	not determinedn.d.	+	-	-	-	-	+	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-

API ID32STA

@ref	URE	ADH (Arg)	ODC	ESC	GLU	FRU	MNE	MAL	LAC	TRE	MAN	RAF	RIB	CEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta GAL	L-arginine arylamidaseArgA	PAL	Pyrrolidonyl arylamidasePyrA	NOVO	SAC	NAG	TUR	ARA	beta GUR
2969	-	-	-	-	+/-	+/-	-	-	-	+/-	-	-	-	-	+	-	-	-	+	-	-	+/-	+/-	-	-	+
2969	+	-	-	-	+	+	-	-	+/-	+	+	-	-	-	+	-	-	-	+/-	-	-	+/-	+	-	-	+
2969	-	-	-	-	+	+	-	-	-	+/-	-	-	-	-	+	-	-	-	-	-	-	+/-	+/-	-	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
2969	fly, body surface		Czech Republic	CZE	Europe
53517	Fly body surface (Stomoxys calcitrans)	Olomouc	Czech Republic	CZE	Europe
121204	Other, Flies trapped in cow sheds		Czech Republic	CZE	Europe

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2969	1	Risk group (German classification) According to TRBA 466
121204	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM301927v1 assembly for Staphylococcus muscae ATCC 49910	complete	GCA_003019275	1294.8	8012112996	1294	95.78
66792	ASM1463496v1 assembly for Staphylococcus muscae CCM 4175	scaffold	GCA_014634965	1294.9	8112490586	1294	69.6
66792	ASM290190v1 assembly for Staphylococcus muscae DSM 7068	scaffold	GCA_002901905	1294.7	2917496769	1294	37.32

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Staphylococcus muscae strain DSM 7068 16S ribosomal RNA gene, partial sequence	AY688079	464	1294
2969	Staphylococcus muscae partial 16S rRNA gene, type strain DSM7068T	FR733703	1537	1294
124043	16S rRNA [Staphylococcus muscae, strain MB4 = CCM 4175, Genomic, 1553 nt].	S83566	1553	1294

GC content

@ref	GC-content (mol%)	Method
2969	41.0	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Staphylococcus muscae strain ATCC 49910
PATRIC: 1294.8

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	microaerophile	98.70	no
125439	gram_stain	BacteriaNetⓘ	positive	87.60	no
125439	motility	BacteriaNetⓘ	no	79.20	no
125439	spore_formation	BacteriaNetⓘ	no	76.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Staphylococcus muscae ATCC 49910
NCBI: GCA_003019275.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	88.78	no
125438	anaerobic	anaerobicⓘ	no	96.24	yes
125438	aerobic	aerobicⓘ	yes	54.35	no
125438	spore-forming	spore-formingⓘ	no	76.22	no
125438	thermophilic	thermophileⓘ	no	94.39	no
125438	flagellated	motile2+ⓘ	no	84.86	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Draft Genome Sequences of 64 Type Strains of 50 Species and 25 Subspecies of the Genus Staphylococcus Rosenbach 1884.	Cole K, Foster D, Russell JE, Golubchik T, Llewelyn M, Wilson DJ, Crook D, Paul J, Modernising Medical Microbiology Consortium.	Microbiol Resour Announc	10.1128/mra.00062-19	2019
Metabolism	Role of the twin-arginine translocation pathway in Staphylococcus.	Biswas L, Biswas R, Nerz C, Ohlsen K, Schlag M, Schafer T, Lamkemeyer T, Ziebandt AK, Hantke K, Rosenstein R, Gotz F.	J Bacteriol	10.1128/jb.00642-09	2009
Phylogeny	Comparative Genomics of Staphylococcus rostri, an Undescribed Bacterium Isolated from Dairy Mastitis.	Klove DC, Farre M, Strube ML, Astrup LB.	Vet Sci	10.3390/vetsci10090530	2023
Phylogeny	Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism of gap gene.	Yugueros J, Temprano A, Sanchez M, Luengo JM, Naharro G.	J Clin Microbiol	10.1128/jcm.39.10.3693-3695.2001	2001
Phylogeny	Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target.	Poyart C, Quesne G, Boumaila C, Trieu-Cuot P.	J Clin Microbiol	10.1128/jcm.39.12.4296-4301.2001	2001
Phylogeny	Comparative study using various methods for identification of Staphylococcus species in clinical specimens.	Layer F, Ghebremedhin B, Moder KA, Konig W, Konig B.	J Clin Microbiol	10.1128/jcm.00226-06	2006
Phylogeny	Staphylococcus muscae, a new species isolated from flies.	Hajek V, Ludwig W, Schleifer KH, Springer N, Zitzelsberger W, Kroppenstedt RM, Kocur M	Int J Syst Bacteriol	10.1099/00207713-42-1-97	1992
Phylogeny	Staphylococcus rostri sp. nov., a haemolytic bacterium isolated from the noses of healthy pigs.	Riesen A, Perreten V	Int J Syst Evol Microbiol	10.1099/ijs.0.012443-0	2009

References

#2969	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7068
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#40749	; Curators of the CIP;
#53517	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 36972
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68371	Automatically annotated from API 50CH acid .
#68375	Automatically annotated from API ID32STA .
#121204	Collection of Institut Pasteur ; Curators of the CIP; CIP 103641
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Staphylococcus muscae (DSM 7068, ATCC 49910, CCM 4175)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Murein

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API 50CHac

API ID32STA

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_003019275

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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