Name: Gracilibacillus halotolerans NN
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1460

Type strain:

Species: Gracilibacillus halotolerans

Strain Designation: NN

Culture col. no.: DSM 11805, JCM 10718, ATCC 700849

Strain history: DSM 11805 <-- M. Wainø strain NN.

NCBI tax ID(s): 74386 (species)

SI-ID 44174

ATCC 700849, DSM 11805, KCTC 3702, JCM 10718

Section

Gracilibacillus halotolerans NN is an obligate aerobe, halotolerant, chemoorganotroph bacterium that forms circular colonies and has a creamy white pigmentation.

colony-forming
Gram-positive
motile
pigmented
rod-shaped
spore-forming
chemoorganotroph
halotolerant
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Gracilibacillus*
	Species	**Gracilibacillus halotolerans**
	Full Scientific Name (LPSN)	Gracilibacillus halotolerans Wainø et al. 1999

Information on morphological and physiological properties Morphology

[Ref.: #23041]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #23041]	Cell length	2.0-5.0 µm

[Ref.: #23041]	Cell width	0.4-0.6 µm

[Ref.: #23041]	Cell shape	rod-shaped

[Ref.: #23041]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %95.443

[Ref.: #23041]	Flagellum arrangement	peritrichous

[Ref.: #23041]	Colony size	2.0 mm
[Ref.: #23041]	Colony color	creamy white
[Ref.: #23041]	Colony shape	circular
[Ref.: #23041]	Incubation period	5 days
[Ref.: #23041]	Cultivation medium used	GSL-2 agar medium

[Ref.: #23041]	Pigment production	yes
[Ref.: #23041]	Pigmentation color	creamy white

Information on culture and growth conditions Culture and growth conditions

[Ref.: #4494]

Culture medium

GRACILIBACILLUS MEDIUM (DSMZ Medium 802)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

[Ref.: #4494]

Culture medium composition

expand / minimize

[Ref.: #23041]

Culture medium

GSL-2 agar medium

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Culture medium growth

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Culture medium

Tris-medium

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Culture medium growth

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #4494]	growth	30 °C
[Ref.: #23041]	growth	6.0-50.0 °C
[Ref.: #23041]	optimum	47.0 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #4494]	Temperature range	mesophilic
[Ref.: #23041]	Temperature range	thermophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #23041]			optimum	7.5
[Ref.: #23041]			growth	5.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23041]

Oxygen tolerance

obligate aerobe

[Ref.: #23041]

Nutrition type

chemoorganotroph

[Ref.: #23041]	Spore description	ellipsoid,terminal,in swollen sporangia
[Ref.: #23041]	Type of spore	endospore
[Ref.: #23041]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %99.999

[Ref.: #23041]

Halophily / tolerance level

halotolerant

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23041]		NaCl		growth	0.0-20.0	%(w/v)
[Ref.: #23041]		NaCl		optimum	0.0	%

[Ref.: #23041]	Murein short key	A31
[Ref.: #23041]	Murein types	A1gamma m-Dpm-direct

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23041]	2-oxoglutarate ChEBI	+	growth
[Ref.: #23041]	acetamide ChEBI	-	growth
[Ref.: #23041]	acetate ChEBI	-	growth
[Ref.: #23041]	acetone ChEBI	-	growth
[Ref.: #23041]	adipate ChEBI	-	growth
[Ref.: #23041]	amylose ChEBI	+	growth
[Ref.: #23041]	anisic acid ChEBI	-	growth
[Ref.: #23041]	arabinose ChEBI	+	growth
[Ref.: #23041]	ascorbate ChEBI	+	growth
[Ref.: #23041]	aspartate ChEBI	-	growth
[Ref.: #23041]	benzamide ChEBI	-	growth
[Ref.: #23041]	benzoate ChEBI	-	growth
[Ref.: #23041]	betaine ChEBI	-	growth
[Ref.: #23041]	butanol ChEBI	-	growth
[Ref.: #23041]	butyrate ChEBI	-	growth
[Ref.: #23041]	casein	-	hydrolysis
[Ref.: #23041]	cellobiose ChEBI	+	growth
[Ref.: #23041]	cellulose ChEBI	-	growth
[Ref.: #23041]	citrate ChEBI	-	growth
[Ref.: #23041]	coconut oil	-	growth
[Ref.: #23041]	crab shell chitin	-	growth
[Ref.: #23041]	D-fructose ChEBI	+	growth
[Ref.: #23041]	D-galactose ChEBI	+	growth
[Ref.: #23041]	D-galacturonic acid ChEBI	+	growth
[Ref.: #23041]	D-gluconate ChEBI	+	growth
[Ref.: #23041]	D-glucose ChEBI	+	builds acid from
[Ref.: #23041]	D-glucuronate ChEBI	+	growth
[Ref.: #23041]	D-mannitol ChEBI	+	builds acid from
[Ref.: #23041]	D-mannose ChEBI	+	growth
[Ref.: #23041]	D-sorbitol ChEBI	-	growth
[Ref.: #23041]	D-trehalose ChEBI	+	growth
[Ref.: #23041]	D-xylose ChEBI	+	builds acid from
[Ref.: #23041]	D-xylose ChEBI	+	growth
[Ref.: #23041]	esculin ChEBI	+	hydrolysis
[Ref.: #23041]	ethanol ChEBI	-	growth
[Ref.: #23041]	ethanolamine ChEBI	-	growth
[Ref.: #23041]	formate ChEBI	-	growth
[Ref.: #23041]	fucose ChEBI	-	growth
[Ref.: #23041]	fumarate ChEBI	-	growth
[Ref.: #23041]	gelatin ChEBI	+	hydrolysis
[Ref.: #23041]	glucose ChEBI	-	fermentation
[Ref.: #23041]	glucose ChEBI	+	growth
[Ref.: #23041]	glutaric acid ChEBI	-	growth
[Ref.: #23041]	glycerol ChEBI	+	growth
[Ref.: #23041]	glycogen ChEBI	+	growth
[Ref.: #23041]	glycolate ChEBI	-	growth
[Ref.: #23041]	glyoxylate ChEBI	-	growth
[Ref.: #23041]	hexanoate ChEBI	-	growth
[Ref.: #23041]	inulin ChEBI	+	growth
[Ref.: #23041]	L-alanine ChEBI	-	growth
[Ref.: #23041]	L-arginine ChEBI	-	growth
[Ref.: #23041]	L-cysteine ChEBI	-	growth
[Ref.: #23041]	L-glutamate ChEBI	-	growth
[Ref.: #23041]	L-lysine ChEBI	-	growth
[Ref.: #23041]	L-malate ChEBI	+	growth
[Ref.: #23041]	L-methionine ChEBI	-	growth
[Ref.: #23041]	L-ornithine ChEBI	-	growth
[Ref.: #23041]	L-phenylalanine ChEBI	-	growth
[Ref.: #23041]	L-proline ChEBI	-	growth
[Ref.: #23041]	L-rhamnose ChEBI	+	growth
[Ref.: #23041]	L-serine ChEBI	-	growth
[Ref.: #23041]	L-threonine ChEBI	-	growth
[Ref.: #23041]	lactate ChEBI	-	growth
[Ref.: #23041]	lactose ChEBI	+	growth
[Ref.: #23041]	maltose ChEBI	+	growth
[Ref.: #23041]	mannitol ChEBI	+	growth
[Ref.: #23041]	melezitose ChEBI	+	growth
[Ref.: #23041]	melibiose ChEBI	+	growth
[Ref.: #23041]	methanol ChEBI	-	growth
[Ref.: #23041]	methylamine ChEBI	-	growth
[Ref.: #23041]	N-acetylglucosamine ChEBI	+	growth
[Ref.: #23041]	nicotinate ChEBI	-	growth
[Ref.: #23041]	nitrate ChEBI	+	reduction
[Ref.: #23041]	octanoate ChEBI	-	growth
[Ref.: #23041]	oil of cedar wood	-	growth
[Ref.: #23041]	pentanol ChEBI	-	growth
[Ref.: #23041]	picolinate ChEBI	-	growth
[Ref.: #23041]	propanol ChEBI	-	growth
[Ref.: #23041]	propionate ChEBI	-	growth
[Ref.: #23041]	pyruvate ChEBI	-	growth
[Ref.: #23041]	raffinose ChEBI	+	growth
[Ref.: #23041]	starch ChEBI	+	growth
[Ref.: #23041]	starch ChEBI	+	hydrolysis
[Ref.: #23041]	succinate ChEBI	-	growth
[Ref.: #23041]	sucrose ChEBI	+	growth
[Ref.: #23041]	sulfanilamide ChEBI	-	growth
[Ref.: #23041]	trimethylamine ChEBI	+	growth
[Ref.: #23041]	tryptophan ChEBI	-	growth
[Ref.: #23041]	tween 80 ChEBI	+	growth
[Ref.: #23041]	tween 80 ChEBI	+	hydrolysis
[Ref.: #23041]	urea ChEBI	+	hydrolysis
[Ref.: #23041]	valerate ChEBI	-	growth
[Ref.: #23041]	xylose ChEBI	-	fermentation
[Ref.: #23041]	yeast extract	+	required for growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #23041]	ampicillin ChEBI		yes
[Ref.: #23041]	bacitracin ChEBI	yes
[Ref.: #23041]	carbenicillin ChEBI	yes
[Ref.: #23041]	chloramphenicol ChEBI	yes
[Ref.: #23041]	erythromycin ChEBI	yes
[Ref.: #23041]	gentamicin ChEBI		yes
[Ref.: #23041]	kanamycin ChEBI		yes
[Ref.: #23041]	nalidixic acid ChEBI		yes
[Ref.: #23041]	neomycin ChEBI		yes
[Ref.: #23041]	novobiocin ChEBI	yes
[Ref.: #23041]	penicillin ChEBI	yes
[Ref.: #23041]	rifampicin ChEBI	yes
[Ref.: #23041]	tetracycline ChEBI		yes
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23041]	acetoin ChEBI	no
[Ref.: #23041]	hydrogen sulfide ChEBI	yes
[Ref.: #23041]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #23041]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #23041]	alkaline phosphatase	+	3.1.3.1
[Ref.: #23041]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23041]	beta-galactosidase	+	3.2.1.23
[Ref.: #23041]	catalase	+	1.11.1.6
[Ref.: #23041]	chitinase	-	3.2.1.14
[Ref.: #23041]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23041]	lecithinase	-
[Ref.: #23041]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23041]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23041]	phenylalanine deaminase	-	4.3.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #23041]	Sample type/isolated from	surface mud
[Ref.: #23041]	Geographic location (country and/or sea, region)	Great Salt Lake,Utah
[Ref.: #23041]	Country	USA
[Ref.: #23041]	Country ISO 3 Code	USA
[Ref.: #23041]	Continent	North America
[Ref.: #23041]	Enrichment culture	liquid GSL-2 medium
[Ref.: #23041]	Enrichment culture composition	containing of (1-1 distilled water): NaCl, 100g; MgSO4.7H2O, 10g; KCl, 5g; NH4Cl, 2g; NaHCO3, 1g; citric acid, 0.5g; yeast extract (Difco), 2g; trypticase peptone (BBL), 2g; trace metal solution (TMS 3), 2ml; KH2PO4 (50 g l-1),10 ml; CaCl2.2H2O (20 g 1-l),10;FeCl2/MnCl2 solution [FeCl2.4H2O (20 gl-1) + MnCl2.4H2O (20 gl-1)], 2ml and Glucose (0.2% w/v)

[Ref.: #67770]	Sample type/isolated from	Surface mud, the Great Salt Lake
[Ref.: #67770]	Geographic location (country and/or sea, region)	UT
[Ref.: #67770]	Country	USA
[Ref.: #67770]	Country ISO 3 Code	USA
[Ref.: #67770]	Continent	North America

[Ref.: #4494]	Geographic location (country and/or sea, region)	Utah, Great Salt Lake
[Ref.: #4494]	Country	USA
[Ref.: #4494]	Country ISO 3 Code	USA
[Ref.: #4494]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Mud (Sludge)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #4494]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #4494]	Gracilibacillus halotolerans 16S ribosomal RNA gene, complete sequence	AF036922	1541	ENA	74386 tax ID
[Ref.: #20218]	Gracilibacillus halotolerans isolate Gh10 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723657	439	ENA	74386 tax ID	*
[Ref.: #20218]	Gracilibacillus halotolerans isolate Gh19 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723658	592	ENA	74386 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Gracilibacillus halotolerans DSM 11805	GCA_014206945	scaffold	GenBank	74386 tax ID	*
[Ref.: #66792]	Gracilibacillus halotolerans strain DSM 11805	74386.3	wgs	PATRIC	74386 tax ID	*
[Ref.: #66792]	Gracilibacillus halotolerans DSM 11805	2829726240	draft	JGI IMG/M	74386 tax ID	*

[Ref.: #23041]	GC-content	38 mol%
[Ref.: #67770]	GC-content	38 mol%	high performance liquid chromatography (HPLC)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Gracilibacillus halotolerans DSM 11805 NCBI: GCA_014206945.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Gracilibacillus halotolerans DSM 11805 NCBI: GCA_014206945.1
[Ref.: #69480]	flagellated	yes	76.968	no
[Ref.: #69480]	gram-positive	yes	93.082	yes
[Ref.: #69480]	anaerobic	no	99.138	yes
[Ref.: #69480]	aerobic	yes	88.302	no
[Ref.: #69480]	halophile	yes	83.057	no
[Ref.: #69480]	spore-forming	yes	94.27	yes
[Ref.: #69480]	glucose-util	yes	89.305	yes
[Ref.: #69480]	thermophile	no	86.072	no
[Ref.: #69480]	motile	yes	88.207	yes
[Ref.: #69480]	glucose-ferment	no	88.197	yes

Availability in culture collections External links

[Ref.: #4494]

Culture collection no.

DSM 11805, JCM 10718, ATCC 700849

[Ref.: #71107]

SI-ID 44174

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.	Waino M, Tindall BJ, Schumann P, Ingvorsen K	Int J Syst Bacteriol	10.1099/00207713-49-2-821	1999	*

References

#4494

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 11805

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23041

Michael Waino,B. J. Tindall,Peter Schumann,Kjeld Ingvorsen: Gracilibacillus gen. nov., with description of Gracilibacillus halotolerans gen. nov., sp. nov.; transfer of Bacillus dipsosauri to Gracilibacillus dipsosauri comb. nov., and Bacillus salexigens to the genus Salibacillus gen. nov., as Salibacillus salexigens comb. nov.. IJSEM 49: 821 - 831 1999 ( DOI 10.1099/00207713-49-2-821 , PubMed 10319508 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#71107

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID44174.1 )

* These data were automatically processed and therefore are not curated

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Gracilibacillus halotolerans DSM 11805

Gracilibacillus halotolerans DSM 11805

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