Staphylococcus simulans (DSM 20322, ATCC 27848, CCM 2705)

Name: Staphylococcus simulans (DSM 20322, ATCC 27848, CCM 2705)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14572

Type strain

Strain Designation MK 148, MK148

Culture col. no. DSM 20322 ATCC 27848 CCM 2705 CCUG 7327 A NCTC 11046 12 more

NCBI tax ID(s) 1286

Special Collections DSMZ CCUG JCM CIP

History DSMZ <- K.H. Schleifer <- W.E. Kloos, MK 148 JCM <-- Y. Kosako <-- R. Sakazaki <-- ATCC 27848 <-- W. E. Kloos MK 148. CIP <- 1981, J. Pillet, Inst. Pasteur, Paris, France <- ATCC <- W.E. Kloos: strain MK148

Links

Staphylococcus simulans MK 148 is an aerobe, Gram-negative, coccus-shaped bacterium that was isolated from human skin.

Gram-negative coccus-shaped aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 39214

ATCC 27848,CCUG 7327,CCM 2705,NCTC 11046,DSM 20322,JCM 2424,CCRC 12928,CIP 81.64,CECT 4538,NRRL B-14753,BCRC 12928,CCUG 7327 A,HAMBI 2058,VTT E-97784,CNCTC 5680

@ref 20215

Last LPSN update 2026-05-29 08:28:19

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Staphylococcaceae

Genus Staphylococcus

Species Staphylococcus simulans

Full scientific name Staphylococcus simulans Kloos and Schleifer 1975 (Approved Lists 1980)

BacDive ID	Other strains from **Staphylococcus simulans** (21)	Type strain
14571	S. simulans DSM 20037, ATCC 12559, CCM 901
14573	S. simulans AW 215, DSM 20323, ATCC 27849, CCM 2724
14574	S. simulans KL 240, DSM 20324, ATCC 27850
14575	S. simulans DSM 20723, NRRL B-2628
24589	S. simulans A7.1, DSM 30972
142831	S. simulans CCUG 15173
146913	S. simulans CCUG 32423
147208	S. simulans CCUG 33053
148040	S. simulans CCUG 34720
150093	S. simulans CCUG 38977
151347	S. simulans CCUG 43809
151513	S. simulans CCUG 44180
152232	S. simulans CCUG 46177
152254	S. simulans CCUG 46217
154559	S. simulans CCUG 54408
154844	S. simulans CCUG 55382
155571	S. simulans CCUG 58188
155850	S. simulans CCUG 59470
156660	S. simulans CCUG 63959
156811	S. simulans CCUG 65537
160182	S. simulans CMS5-9-3, DSM 24884

Morphology

Cell morphology

@ref	Gram stain	Cell shape	Motility
122626	negative	coccus-shaped

Colony morphology

@ref	Type of hemolysis	Hemolysis ability
8713	beta	1
122626		1

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8713	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
8713	TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)	Medium recipe at MediaDive	Name: TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92) Composition: Trypticase soy broth 30.0 g/l Agar 15.0 g/l Yeast extract 3.0 g/l Distilled water
34889	MEDIUM 3 - Columbia agar		Columbia agar (39.000 g);distilled water (1000.000 ml)
122626	CIP Medium 3	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
8713	positive	growth	37
34889	positive	growth	37
44978	positive	growth	37
67770	positive	growth	37
122626	positive	growth	10-45

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
44978	aerobe
122626	facultative anaerobe
125439	facultative anaerobe	94.39

Antibiotic susceptibility testing

	Test 1	Test 2	Test 3	Test 4
@ref	8713	8713	8713	8713
Medium	Mueller-Hinton Agar	Mueller-Hinton Agar	Mueller-Hinton Agar	Müller-Hinton Agar
Manual annotation	1	1	1	1
	Inhibition zone diameter in mm	Inhibition zone diameter in mm	Inhibition zone diameter in mm	Inhibition zone diameter in mm
Amikacin 30µg (disc)	34	32	32	36
Ampicillin 10µg (disc)	38	30	30	28
Aztreonam 30µg (disc)	0	0	0	0
Bacitracin 10Unit	20-22	18	16-18	n.d.
Cefalotin 30µg (disc)	44	40	38	n.d.
Cefazolin 30µg (disc)	42	40	36-38	n.d.
Cefiderocol 30µg (disc)	n.d.	n.d.	n.d.	16-18
Cefotaxime 30µg (disc)	36-38	32-34	30	32
Ceftazidime 10µg (disc)	n.d.	n.d.	n.d.	22
Ceftriaxone 30µg (disc)	34	n.d.	n.d.	30
Chloramphenicol 30µg (disc)	32	30-32	30	36
Ciprofloxacin 5µg (disc)	n.d.	n.d.	n.d.	42
Clindamycin 10µg (disc)	42	n.d.	n.d.	46
Colistin 10µg (disc)	12	14	12-14	n.d.
Colistin sulphate 10µg (disc)	n.d.	n.d.	n.d.	16
Doxycycline 30µg (disc)	18	18	18	n.d.
Erythromycin 15µg (disc)	32-34	32	32	38
Fosfomycin 50µg (disc)	>50	n.d.	n.d.	44
Gentamicin 10µg (disc)	32-34	30-32	32	n.d.
Gentamicin 30µg (disc)	n.d.	n.d.	n.d.	38
Imipenem 10µg (disc)	>50	>50	50	>50
Kanamycin 30µg (disc)	36	42-44	30	36
Levofloxacin 5µg (disc)	n.d.	n.d.	n.d.	40
Lincomycin 15µg (disc)	38	34	36	n.d.
Linezolid 10µg (disc)	38	n.d.	n.d.	42
Meropenem 10µg (disc)	n.d.	n.d.	n.d.	46-48
Mezlocillin 30µg (disc)	34	30	28	n.d.
Moxifloxacin 5µg (disc)	40	n.d.	n.d.	42
Neomycin 30µg (disc)	28	28	28	n.d.
Nitrofurantoin 100µg (disc)	30-32	26	26	26
Norfloxacin 10µg (disc)	36	38-40	36	n.d.
Nystatin 100Unit	0	n.d.	n.d.	n.d.
Ofloxacin 5µg (disc)	34	32	32	40
Oxacillin 5µg (disc)	38	32	32	38
Penicillin G 6µg (disc)	34	26-28	26	26
Pipemidic acid 20µg (disc)	22	22	22	n.d.
Piperacillin/Tazobactam 40µg (disc)	34-36	n.d.	n.d.	n.d.
Piperacillin/Tazobactam 110µg (disc)	n.d.	n.d.	n.d.	32
Polymyxin B 300Unit	16	16	14	18
Quinupristin/Dalfopristin 15µg (disc)	32	n.d.	n.d.	34
Rifampicin 5µg (disc)	n.d.	n.d.	n.d.	42
Teicoplanin 30µg (disc)	22-24	n.d.	n.d.	22
Tetracycline 30µg (disc)	10	10	10	12
Ticarcillin 75µg (disc)	38-40	32-34	30	30
Tigecycline 15µg (disc)	n.d.	n.d.	n.d.	32
Trimethoprim-sulfamethoxazole (1:19) 10µg (disc)	n.d.	n.d.	n.d.	28
Vancomycin 30µg (disc)	22-24	20-22	22	24

Spore formation

@ref	Spore formation	Confidence
125439		99.27

Murein

@ref	Murein short key	Type
8713	A11.02	A3alpha L-Lys-Gly_5-6

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68375	22599 ChEBI	arabinose	-	fermentation	from API ID32STA
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68375	15824 ChEBI	D-fructose	+	fermentation	from API ID32STA
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68375	17634 ChEBI	D-glucose	+	fermentation	from API ID32STA
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68375	16899 ChEBI	D-mannitol	+	fermentation	from API ID32STA
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	-	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68375	4853 ChEBI	esculin	-	hydrolysis	from API ID32STA
68371	4853 ChEBI	esculin	-	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68371	28087 ChEBI	glycogen	-	builds acid from	from API 50CH acid
122626	606565 ChEBI	hippurate	+	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68375	17716 ChEBI	lactose	+	fermentation	from API ID32STA
68371	17716 ChEBI	lactose	+	builds acid from	from API 50CH acid
68371	17306 ChEBI	maltose	-	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68375	59640 ChEBI	N-acetylglucosamine	+	fermentation	from API ID32STA
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
68375	17632 ChEBI	nitrate	+	reduction	from API ID32STA
122626	17632 ChEBI	nitrate	+	reduction
122626	16301 ChEBI	nitrite	-	reduction
68375	18257 ChEBI	ornithine	-	degradation	from API ID32STA
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68375	16634 ChEBI	raffinose	-	fermentation	from API ID32STA
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68371	28017 ChEBI	starch	-	builds acid from	from API 50CH acid
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68375	17992 ChEBI	sucrose	+	fermentation	from API ID32STA
68375	27082 ChEBI	trehalose	+	fermentation	from API ID32STA
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68375	32528 ChEBI	turanose	-	fermentation	from API ID32STA
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68375	16199 ChEBI	urea	+	hydrolysis	from API ID32STA
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	ChEBI	Metabolite	Is resistant	Resistance conc.
8713	161680	Aztreonam		30 µg (disc)	from Antibiotic test
8713	7660	Nystatin		100 Unit	from Antibiotic test

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68375	15688 ChEBI	acetoin		from API ID32STA
122626	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
68375	15688 ChEBI	acetoin	-		from API ID32STA
122626	15688 ChEBI	acetoin	-
122626	17234 ChEBI	glucose		+

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
122626	alcohol dehydrogenase	+	1.1.1.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
122626	amylase	-
68382	beta-galactosidase	+	3.2.1.23	from API zym
122626	beta-galactosidase	+	3.2.1.23
68375	beta-galactosidase	+	3.2.1.23	from API ID32STA
68382	beta-glucosidase	-	3.2.1.21	from API zym
68375	beta-glucosidase	-	3.2.1.21	from API ID32STA
68382	beta-glucuronidase	+	3.2.1.31	from API zym
68375	beta-glucuronidase	+	3.2.1.31	from API ID32STA
122626	caseinase	-	3.4.21.50
8713	catalase	+	1.11.1.6
122626	catalase	+	1.11.1.6
122626	coagulase	-
68382	cystine arylamidase	-	3.4.11.3	from API zym
8713	cytochrome-c oxidase	-	1.9.3.1
122626	DNase	+/-
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
122626	gamma-glutamyltransferase	-	2.3.2.2
122626	gelatinase	-
68375	L-arginine arylamidase	-		from API ID32STA
122626	lecithinase	+
68382	leucine arylamidase	-	3.4.11.1	from API zym
122626	lipase	-
68382	lipase (C 14)	-		from API zym
122626	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
122626	ornithine decarboxylase	-	4.1.1.17
68375	ornithine decarboxylase	-	4.1.1.17	from API ID32STA
122626	oxidase	-
122626	phenylalanine ammonia-lyase	-	4.3.1.24
122626	protease	+
68382	trypsin	-	3.4.21.4	from API zym
122626	tween esterase	-
122626	urease	+	3.5.1.5
68375	urease	+	3.5.1.5	from API ID32STA
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
122626	-	-	+	+	-	-	-	-	-	-	+	-	-	+	+	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
122626	not determinedn.d.	-	-	-	-	+/-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	+	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API ID32STA

@ref	URE	ADH (Arg)	ODC	ESC	GLU	FRU	MNE	MAL	LAC	TRE	MAN	RAF	RIB	CEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta GAL	L-arginine arylamidaseArgA	PAL	Pyrrolidonyl arylamidasePyrA	NOVO	SAC	NAG	TUR	ARA	beta GUR
8713	+	+	-	-	+	+	-	-	+	+	+	-	-	-	+	-	+	-	-	+	-	+	+	-	-	+
8713	+	+	-	-	+	+	-	-	+	+	+	-	+/-	-	+	-	+	-	+/-	+	+/-	+	+	-	-	+
8713	+	+	-	-	+	+	-	-	+	+	+	-	-	-	+	-	+	-	+	+	+	+	+	-	-	+
8713	+	+	-	-	+	+	+	+	+	+	+	-	+	+	+	-	+	-	-	+	+/-	+	+	-	-	+
8713	+	-	-	-	+	+	-	-	+	+	+	-	+	-	+	-	+	-	+	-	+	+	+	-	-	+
8713	+	+	-	-	+	+	-	-	+	+	+	-	+	-	+	-	+	-	+	+	+	+	+	-	-	+
8713	+	+	-	-	+	+	-	-	+	+	+	-	+	-	+	-	+	-	+	+	+	+	+	-	-	+
8713	+	+	-	-	+	+	-	-	+	+	+	-	+	-	+	-	+	-	+/-	+	-	+	+	-	-	+

API biotype100

@ref	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
122626	not determinedn.d.	+	+	-	+	-	-	-	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	-
#Host Body-Site	#Organ	#Skin, Nail, Hair

Isolation

@ref	Sample type	Host species
8713	human skin	Homo sapiens
44978	Human skin	Homo sapiens
67770	Human skin	Homo sapiens
122626	Human, Skin	Homo sapiens

Taxonmaps

69479

Global distribution of 16S sequence D83373 (>99% sequence identity) for Staphylococcus from Microbeatlas

Aquatic Samples	18
Soil Samples	17
Animal Samples	249
Plant Samples	12
Total Samples	296

Interaction and safety

Risk assessment

@ref	Pathogenicity human	Pathogenicity animal	Biosafety level	Biosafety level comment
8713	yes, in single cases	yes, in single cases	1	Risk group (German classification) According to TRBA 466
122626			1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	40961_B01 assembly for Staphylococcus simulans NCTC11046	complete	GCA_900474685	1286.118	8113754107	1286	97.66
66792	Simulans assembly for Staphylococcus simulans	chromosome	GCA_900240105	1286.80	2986849160	1286	82.14
67770	ASM290228v1 assembly for Staphylococcus simulans NCTC 11046	scaffold	GCA_002902285	1286.76	8053145011	1286	56.54

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
20218	Staphylococcus simulans strain DSM 20322 16S ribosomal RNA gene, partial sequence	AY688099	464		1286
8713	Staphylococcus simulans gene for 16S rRNA, partial sequence, strain: ATCC 27848 (= MAFF 910161)	D83373	1476		1286

GC content

@ref	GC-content (mol%)	Method
8713	34.0
67770	34	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: 40961_B01 assembly for Staphylococcus simulans NCTC11046
BV-BRC: 1286.118

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	94.39	no
125439	gram_stain	BacteriaNetⓘ	positive	51.50	no
125439	motility	BacteriaNetⓘ	no	81.15	no
125439	spore_formation	BacteriaNetⓘ	no	99.27	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Staphylococcus simulans NCTC11046
NCBI: GCA_900474685.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	88.37	no
125438	anaerobic	anaerobicⓘ	no	98.80	yes
125438	aerobic	aerobicⓘ	yes	51.47	no
125438	spore-forming	spore-formingⓘ	no	70.06	no
125438	thermophilic	thermophileⓘ	no	94.50	yes
125438	flagellated	motile2+ⓘ	no	80.91	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages.	Cocolin L, Manzano M, Cantoni C, Comi G.	Appl Environ Microbiol	10.1128/aem.67.11.5113-5121.2001	2001
	Use of Ozone for Disinfection of PHARMODUCT^® Automatic System for Antineoplastic Compounding.	Lovino V, Riglietti A, Tolomeo A, Capasso G, Di Vittorio M, Brattoli S, Tesse G, Dimiccoli V, Sparta M, Perioli L.	Pharmaceuticals (Basel)	10.3390/ph18020140	2025
Enzymology	Cloning and expression of Staphylococcus simulans lysostaphin enzyme gene in Bacillus subtilis WB600.	Far BE, Ragheb M, Rahbar R, Mafakher L, Nojookambari NY, Achinas S, Yazdansetad S.	AIMS Microbiol	10.3934/microbiol.2021017	2021
	Species identification by MALDI-TOF MS and gap PCR-RFLP of non-aureus Staphylococcus, Mammaliicoccus, and Streptococcus spp. associated with sheep and goat mastitis.	Rosa NM, Penati M, Fusar-Poli S, Addis MF, Tola S.	Vet Res	10.1186/s13567-022-01102-4	2022
	Susceptibility to Bacteriocins in Biofilm-Forming, Variable Staphylococci Isolated from Local Slovak Ewes' Milk Lump Cheeses.	Laukova A, Pogany Simonova M, Fockova V, Kolosta M, Tomaska M, Dvoroznakova E.	Foods	10.3390/foods9091335	2020
	Novel Peptide from Commensal Staphylococcus simulans Blocks Methicillin-Resistant Staphylococcus aureus Quorum Sensing and Protects Host Skin from Damage.	Brown MM, Kwiecinski JM, Cruz LM, Shahbandi A, Todd DA, Cech NB, Horswill AR.	Antimicrob Agents Chemother	10.1128/aac.00172-20	2020
Metabolism	Pathogenic conversion of coagulase-negative staphylococci.	Yu W, Kim HK, Rauch S, Schneewind O, Missiakas D.	Microbes Infect	10.1016/j.micinf.2016.12.002	2017
Enzymology	Performance of SaSelect, a chromogenic medium for detection of staphylococci in clinical specimens.	Hirvonen JJ, Kerttula AM, Kaukoranta SS.	J Clin Microbiol	10.1128/jcm.03129-13	2014
Phylogeny	Detection of methicillin-resistant Staphylococcus aureus by a duplex droplet digital PCR assay.	Kelley K, Cosman A, Belgrader P, Chapman B, Sullivan DC.	J Clin Microbiol	10.1128/jcm.00196-13	2013
Phylogeny	Evaluation of a triplex PCR assay to discriminate Staphylococcus aureus from coagulase-negative Staphylococci and determine methicillin resistance from blood cultures.	Maes N, Magdalena J, Rottiers S, De Gheldre Y, Struelens MJ.	J Clin Microbiol	10.1128/jcm.40.4.1514-1517.2002	2002
Phylogeny	Glyceraldehyde-3-phosphate dehydrogenase-encoding gene as a useful taxonomic tool for Staphylococcus spp.	Yugueros J, Temprano A, Berzal B, Sanchez M, Hernanz C, Luengo JM, Naharro G.	J Clin Microbiol	10.1128/jcm.38.12.4351-4355.2000	2000
Enzymology	Development of a rapid PCR assay specific for Staphylococcus saprophyticus and application to direct detection from urine samples.	Martineau F, Picard FJ, Menard C, Roy PH, Ouellette M, Bergeron MG.	J Clin Microbiol	10.1128/jcm.38.9.3280-3284.2000	2000
Enzymology	Microbial quality and direct PCR identification of lactic acid bacteria and nonpathogenic Staphylococci from artisanal low-acid sausages.	Aymerich T, Martin B, Garriga M, Hugas M.	Appl Environ Microbiol	10.1128/aem.69.8.4583-4594.2003	2003
	Identification of a methicillin-resistant strain of Staphylococcus caprae from a human clinical specimen.	Kanda K, Suzuki E, Hiramatsu K, Oguri T, Miura H, Ezaki T, Yokota T.	Antimicrob Agents Chemother	10.1128/aac.35.1.174	1991
Phylogeny	Evaluation of a rapid direct assay for identification of bacteria and the mec A and van genes from positive-testing blood cultures.	Eigner U, Weizenegger M, Fahr AM, Witte W.	J Clin Microbiol	10.1128/jcm.43.10.5256-5262.2005	2005
Pathogenicity	Real-time PCR assay for detection of fluoroquinolone resistance associated with grlA mutations in Staphylococcus aureus.	Lapierre P, Huletsky A, Fortin V, Picard FJ, Roy PH, Ouellette M, Bergeron MG.	J Clin Microbiol	10.1128/jcm.41.7.3246-3251.2003	2003
	epr, which encodes glycylglycine endopeptidase resistance, is homologous to femAB and affects serine content of peptidoglycan cross bridges in Staphylococcus capitis and Staphylococcus aureus.	Sugai M, Fujiwara T, Ohta K, Komatsuzawa H, Ohara M, Suginaka H.	J Bacteriol	10.1128/jb.179.13.4311-4318.1997	1997
Metabolism	EssD, a Nuclease Effector of the Staphylococcus aureus ESS Pathway.	Ohr RJ, Anderson M, Shi M, Schneewind O, Missiakas D.	J Bacteriol	10.1128/jb.00528-16	2017
Phylogeny	Development of a PCR assay for identification of staphylococci at genus and species levels.	Martineau F, Picard FJ, Ke D, Paradis S, Roy PH, Ouellette M, Bergeron MG.	J Clin Microbiol	10.1128/jcm.39.7.2541-2547.2001	2001
Enzymology	Evaluation of the Staph-Zym system with staphylococci isolated from bovine intramammary infections.	Watts JL, Washburn PJ.	J Clin Microbiol	10.1128/jcm.29.1.59-61.1991	1991
Enzymology	Use of tuf sequences for genus-specific PCR detection and phylogenetic analysis of 28 streptococcal species.	Picard FJ, Ke D, Boudreau DK, Boissinot M, Huletsky A, Richard D, Ouellette M, Roy PH, Bergeron MG.	J Clin Microbiol	10.1128/jcm.42.8.3686-3695.2004	2004
Enzymology	Rapid concentration and molecular enrichment approach for sensitive detection of Escherichia coli and Shigella species in potable water samples.	Maheux AF, Bissonnette L, Boissinot M, Bernier JL, Huppe V, Picard FJ, Berube E, Bergeron MG.	Appl Environ Microbiol	10.1128/aem.02337-10	2011
Phylogeny	Lysostaphin disk test for routine presumptive identification of staphylococci.	Poutrel B, Caffin JP.	J Clin Microbiol	10.1128/jcm.13.6.1023-1025.1981	1981
	Development of a PCR assay for rapid detection of enterococci.	Ke D, Picard FJ, Martineau F, Menard C, Roy PH, Ouellette M, Bergeron MG.	J Clin Microbiol	10.1128/jcm.37.11.3497-3503.1999	1999
Enzymology	Species-specific and ubiquitous-DNA-based assays for rapid identification of Staphylococcus aureus.	Martineau F, Picard FJ, Roy PH, Ouellette M, Bergeron MG.	J Clin Microbiol	10.1128/jcm.36.3.618-623.1998	1998
Metabolism	Choriogonadotropin-like antigen in a strain of Streptococcus faecalis and a strain of Staphylococcus simulans: detection, identification, and characterization.	Acevedo HF, Koide SS, Slifkin M, Maruo T, Campbell-Acevedo EA.	Infect Immun	10.1128/iai.31.1.487-494.1981	1981
Phylogeny	Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target.	Poyart C, Quesne G, Boumaila C, Trieu-Cuot P.	J Clin Microbiol	10.1128/jcm.39.12.4296-4301.2001	2001
Metabolism	Role of the twin-arginine translocation pathway in Staphylococcus.	Biswas L, Biswas R, Nerz C, Ohlsen K, Schlag M, Schafer T, Lamkemeyer T, Ziebandt AK, Hantke K, Rosenstein R, Gotz F.	J Bacteriol	10.1128/jb.00642-09	2009
Phylogeny	Evaluation of Staph ID 32 system and Staph-Zym system for identification of coagulase-negative staphylococci.	Renneberg J, Rieneck K, Gutschik E.	J Clin Microbiol	10.1128/jcm.33.5.1150-1153.1995	1995
Phylogeny	Identification of Staphylococcus species and subspecies by the chaperonin 60 gene identification method and reverse checkerboard hybridization.	Goh SH, Santucci Z, Kloos WE, Faltyn M, George CG, Driedger D, Hemmingsen SM.	J Clin Microbiol	10.1128/jcm.35.12.3116-3121.1997	1997
	Evaluation of the Minitek gram-positive set for identification of staphylococci isolated from the bovine mammary gland.	Watts JL, Owens WE, Nickerson SC.	J Clin Microbiol	10.1128/jcm.23.5.873-875.1986	1986
Phylogeny	Rapid test for the serological separation of staphylococci from micrococci.	Seidl PH, Schleifer KH.	Appl Environ Microbiol	10.1128/aem.35.3.479-482.1978	1978
	Novel staphylococcal glycosyltransferases SdgA and SdgB mediate immunogenicity and protection of virulence-associated cell wall proteins.	Hazenbos WL, Kajihara KK, Vandlen R, Morisaki JH, Lehar SM, Kwakkenbos MJ, Beaumont T, Bakker AQ, Phung Q, Swem LR, Ramakrishnan S, Kim J, Xu M, Shah IM, Diep BA, Sai T, Sebrell A, Khalfin Y, Oh A, Koth C, Lin SJ, Lee BC, Strandh M, Koefoed K, Andersen PS, Spits H, Brown EJ, Tan MW, Mariathasan S.	PLoS Pathog	10.1371/journal.ppat.1003653	2013
Phylogeny	Use of trehalose-mannitol-phosphatase agar to differentiate Staphylococcus epidermidis and Staphylococcus saprophyticus from other coagulase-negative staphylococci.	Stevens DL, Jones C.	J Clin Microbiol	10.1128/jcm.20.5.977-980.1984	1984
Enzymology	Evaluation of the Staph-Ident and STAPHase systems for identification of staphylococci from bovine intramammary infections.	Watts JL, Pankey JW, Nickerson SC.	J Clin Microbiol	10.1128/jcm.20.3.448-452.1984	1984
Phylogeny	Comparison of methods for identifying Staphylococcus and Micrococcus spp.	Gunn BA, Singleton FL, Peele ER, Colwell RR, Keiser JK, Kapper CO.	J Clin Microbiol	10.1128/jcm.14.2.195-200.1981	1981
Metabolism	Intracellular mannitol, a product of glucose metabolism in staphylococci.	Edwards KG, Blumenthal HJ, Khan M, Slodki ME.	J Bacteriol	10.1128/jb.146.3.1020-1029.1981	1981
Phylogeny	Characterization of clinically significant strains of coagulase-negative staphylococci.	Christensen GD, Parisi JT, Bisno AL, Simpson WA, Beachey EH.	J Clin Microbiol	10.1128/jcm.18.2.258-269.1983	1983
Phylogeny	Comparative study using various methods for identification of Staphylococcus species in clinical specimens.	Layer F, Ghebremedhin B, Moder KA, Konig W, Konig B.	J Clin Microbiol	10.1128/jcm.00226-06	2006
Phylogeny	Simplified lyogroup system, a new method for routine identification of staphylococci: description and comparison with three other methods.	Varaldo PE, Grazi G, Soro O, Cisani G, Satta G.	J Clin Microbiol	10.1128/jcm.12.1.63-68.1980	1980
	Draft Genome Sequences of 64 Type Strains of 50 Species and 25 Subspecies of the Genus Staphylococcus Rosenbach 1884.	Cole K, Foster D, Russell JE, Golubchik T, Llewelyn M, Wilson DJ, Crook D, Paul J, Modernising Medical Microbiology Consortium.	Microbiol Resour Announc	10.1128/mra.00062-19	2019
	Lysyl-Phosphatidylglycerol: A Lipid Involved in the Resistance of Staphylococcus aureus to Antimicrobial Peptide Activity.	Vasquez A, Leidy C, Manrique-Moreno M.	Antibiotics (Basel)	10.3390/antibiotics14040349	2025
Enzymology	A robotic DNA purification protocol and real-time PCR for the detection of Enterobacter sakazakii in powdered infant formulae.	Derzelle S, Dilasser F.	BMC Microbiol	10.1186/1471-2180-6-100	2006
	By-product hazelnut seed skin characteristics and properties in terms of use in food processing and human nutrition.	Kruk M, Ponder A, Horoszewicz J, Poplawski D, Krol K, Leszczynska J, Jaworska D, Trzaskowska M.	Sci Rep	10.1038/s41598-024-69900-8	2024
	A polypeptide antigen from a strain of Staphylococcus simulans. 2. Antigenic and biological properties.	Osland A, Sveen K	Acta Pathol Microbiol Scand B	10.1111/j.1699-0463.1981.tb00176_89b.x	1981

References

#8713	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20322
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34889	; Curators of the CIP;
#44978	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 7327 A
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68375	Automatically annotated from API ID32STA .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#122626	Collection of Institut Pasteur ; Curators of the CIP; CIP 81.64
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Staphylococcus simulans (DSM 20322, ATCC 27848, CCM 2705)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Antibiotic susceptibility testing

Spore formation

Murein

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

API ID32STA

API biotype100

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_900474685

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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