Name: Staphylococcus schleiferi N850274
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14568

Type strain:

Species: Staphylococcus schleiferi

Strain Designation: N850274, N 850274

Culture col. no.: DSM 4807, ATCC 43808, CCUG 25351, NCTC 12218, CCM 4070, CIP 103643

Strain history: CIP <- 1993, J. Freney, Lyon Med. Univ., Lyon, France: strain N 850274 <- J. Fleurette, E. Herriot Hosp., Lyon, France

NCBI tax ID(s): 1295 (species)

SI-ID 7699

LMG 13347, ATCC 43808, DSM 4807, CCUG 25351, CIP 103643, CCM 4070, NCTC 12218, BCRC 13972, CCRC 13972, KACC 10789, NRRL B-14775

Other strains from Staphylococcus schleiferi

S. schleiferi CCUG 25352
S. schleiferi CCUG 25353
S. schleiferi CCUG 47397
S. schleiferi CCUG 64084

Section

Staphylococcus schleiferi N850274 is an aerobe, mesophilic, Gram-positive human pathogen that was isolated from jugular catheter.

Gram-positive
aerobe
human pathogen
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Staphylococcaceae*
	Genus	*Staphylococcus*
	Species	**Staphylococcus schleiferi**
	Full Scientific Name (LPSN)	Staphylococcus schleiferi Freney et al. 1988

Information on morphological and physiological properties Morphology

[Ref.: #122511]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #122511]	Cell shape	coccus-shaped

[Ref.: #122511]	Motility	no

[Ref.: #122511]

Hemolysis ability

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1830]

Culture medium

COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)

[Ref.: #1830]

Culture medium growth

[Ref.: #1830]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1830]

Culture medium composition

expand / minimize

[Ref.: #1830]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #1830]

Culture medium growth

[Ref.: #1830]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1830]

Culture medium composition

expand / minimize

[Ref.: #34406]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #34406]

Culture medium growth

[Ref.: #34406]

Culture medium composition

expand / minimize

[Ref.: #122511]

Culture medium

CIP Medium 3

[Ref.: #122511]

Culture medium growth

[Ref.: #122511]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #122511]

Culture medium

CIP Medium 72

[Ref.: #122511]

Culture medium growth

[Ref.: #122511]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #1830]		growth	37 °C
[Ref.: #34406]		growth	37 °C
[Ref.: #48178]		growth	37 °C
[Ref.: #122511]		growth	30-45 °C
[Ref.: #122511]	no	growth	15 °C

[Ref.: #1830]	Temperature range	mesophilic
[Ref.: #34406]	Temperature range	mesophilic
[Ref.: #48178]	Temperature range	mesophilic
[Ref.: #122511]	Temperature range	psychrophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #48178]	Oxygen tolerance	aerobe
[Ref.: #122511]	Oxygen tolerance	facultative anaerobe

[Ref.: #69480]

Ability of spore formation

no Confidence in %99.837

[Ref.: #1830]	Murein short key	A11.02
[Ref.: #1830]	Murein types	A3alpha L-Lys-Gly_5-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68375]	arabinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	arginine ChEBI	+	hydrolysis	* from API ID32STA
[Ref.: #68375]	cellobiose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	D-fructose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	D-glucose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	D-mannitol ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	D-mannose ChEBI	+	fermentation	* from API ID32STA
[Ref.: #68375]	D-ribose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	esculin ChEBI	-	hydrolysis	* from API ID32STA
[Ref.: #68375]	lactose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	maltose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #122511]	nitrate ChEBI	+	reduction
[Ref.: #68375]	nitrate ChEBI	+	reduction	* from API ID32STA
[Ref.: #122511]	nitrite ChEBI	-	reduction
[Ref.: #68375]	ornithine ChEBI	-	degradation	* from API ID32STA
[Ref.: #68375]	raffinose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	sucrose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	trehalose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #122511]	tributyrin ChEBI	-	hydrolysis
[Ref.: #68375]	turanose ChEBI	-	fermentation	* from API ID32STA
[Ref.: #68375]	urea ChEBI	-	hydrolysis	* from API ID32STA
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #68375]		novobiocin ChEBI	yes	1.8	µg	* from API ID32STA

	Metabolite production	Metabolite	Production
[Ref.: #122511]		indole ChEBI	no
[Ref.: #68375]		acetoin ChEBI	yes	* from API ID32STA

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #122511]		acetoin ChEBI	-
[Ref.: #68375]		acetoin ChEBI	+	* from API ID32STA

	Enzyme	Enzyme activity	EC number
[Ref.: #122511]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68375]	alkaline phosphatase	+	3.1.3.1	* from API ID32STA
[Ref.: #68375]	arginine dihydrolase	+	3.5.3.6	* from API ID32STA
[Ref.: #122511]	beta-galactosidase	+	3.2.1.23
[Ref.: #68375]	beta-galactosidase	+	3.2.1.23	* from API ID32STA
[Ref.: #68375]	beta-glucosidase	-	3.2.1.21	* from API ID32STA
[Ref.: #68375]	beta-glucuronidase	-	3.2.1.31	* from API ID32STA
[Ref.: #122511]	catalase	+	1.11.1.6
[Ref.: #122511]	coagulase	-
[Ref.: #122511]	DNase	+
[Ref.: #122511]	gelatinase	+/-
[Ref.: #68375]	L-arginine arylamidase	-		* from API ID32STA
[Ref.: #122511]	lecithinase	+
[Ref.: #122511]	lipase	+
[Ref.: #122511]	lysine decarboxylase	-	4.1.1.18
[Ref.: #122511]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #68375]	ornithine decarboxylase	-	4.1.1.17	* from API ID32STA
[Ref.: #122511]	oxidase	-
[Ref.: #68375]	pyrrolidonyl arylamidase	+	3.4.19.3	* from API ID32STA
[Ref.: #122511]	urease	-	3.5.1.5
[Ref.: #68375]	urease	-	3.5.1.5	* from API ID32STA
	Only first 10 entries are displayed. Click here to see all.

API® ID32STA:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Urease/urea hydrolysisURE	Arginine dihydrolaseADH (Arg)	Ornithine decarboxylaseODC	Esculin hydrolysisESC	Fermentation of D-glucoseGLU	Fermentation of D-fructoseFRU	Fermentation of D-mannoseMNE	Fermentation of maltoseMAL	Fermentation of lactoseLAC	Fermentation of trehaloseTRE	Fermentation of D-mannitolMAN	Fermentation of raffinoseRAF	Fermentation of D-riboseRIB	Fermentation of cellobioseCEL	Reduction of nitrateNIT	Acetoin production (Voges Proskauer test)VP	beta-Galactosidasebeta GAL	L-arginine arylamidaseArgA	Alkaline phosphatasePAL	Pyrrolidonyl arylamidasePyrA	Novobiocin resistanceNOVO	Fermentation of sucroseSAC	Fermentation of N-acetyl-glucosamineNAG	Fermentation of turanoseTUR	Fermentation of arabinoseARA	beta-Glucuronidasebeta GUR
[Ref.: #1830]	6230	-	+	-	-	+	+	+	-	-	-	-	-	-	-	+	+	+	-	+	+	-	-	+/-	-	-	-
[Ref.: #1830]	5805	-	+	-	-	+	+	+	-	-	-	-	-	-	-	+	+	+	-	+	+	-	-	+	-	-	-
[Ref.: #1830]	5804	-	+	-	-	+	+	+	-	-	-	-	-	-	-	+	+	+	-	+	+	-	-	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1830]	Sample type/isolated from	jugular catheter

[Ref.: #48178]	Sample type/isolated from	Jugular catheter
[Ref.: #48178]	Geographic location (country and/or sea, region)	Lyon ?
[Ref.: #48178]	Country	France
[Ref.: #48178]	Country ISO 3 Code	FRA
[Ref.: #48178]	Continent	Europe

[Ref.: #122511]	Sample type/isolated from	Other, Jugular catheter
[Ref.: #122511]	Geographic location (country and/or sea, region)	Lyon
[Ref.: #122511]	Country	France
[Ref.: #122511]	Country ISO 3 Code	FRA
[Ref.: #122511]	Continent	Europe
[Ref.: #122511]	Isolation date	1985
* marker position based on {}

Isolation sources categories

#Infection	#Medical device	#Catheter
#Host Body-Site	#Organ	#Vascular system

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1830]	Pathogenicity (human)	yes
[Ref.: #1830]	Pathogenicity (animal)	yes
[Ref.: #1830]	Biosafety level	2	Risk group (German classification) According to TRBA 466
[Ref.: #122511]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #1830]	16S rRNA [Staphylococcus schleiferi, Genomic, 1554 nt]	S83568	1554	ENA	1295 tax ID
[Ref.: #20218]	Staphylococcus schleiferi subsp. schleiferi strain ATCC 43808 16S ribosomal RNA gene, partial sequence	EU157299	366	ENA	74707 tax ID	*
[Ref.: #20218]	S.schleiferi gene for 16S ribosomal RNA	Z26904	771	ENA	1295 tax ID	*
[Ref.: #20218]	Staphylococcus schleiferi subsp. schleiferi strain DSM 4807 16S ribosomal RNA gene, partial sequence	AY688092	464	ENA	74707 tax ID	*

Genome sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Staphylococcus schleiferi ATCC 43808	GCA_011137195	contig	GenBank	1295 tax ID	*
[Ref.: #66792]	Staphylococcus schleiferi subsp. schleiferi NCTC 12218	GCA_003968865	scaffold	GenBank	74707 tax ID	*
[Ref.: #66792]	Staphylococcus schleiferi strain ATCC 43808	1295.80	wgs	PATRIC	1295 tax ID	*
[Ref.: #66792]	Staphylococcus schleiferi strain NCTC12218	1295.64	wgs	PATRIC	1295 tax ID	*
[Ref.: #66792]	Staphylococcus schleiferi subsp. schleiferi strain NCTC 12218	74707.3	wgs	PATRIC	74707 tax ID	*
[Ref.: #66792]	Staphylococcus schleiferi NCTC12218	GCA_900458895	complete	GenBank	1295 tax ID	*

[Ref.: #1830]

GC-content

37.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Staphylococcus schleiferi NCTC12218 NCBI: GCA_900458895.2
[Ref.: #69481]	spore-forming	no	62	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Staphylococcus schleiferi NCTC12218 NCBI: GCA_900458895.1
[Ref.: #69480]	flagellated	no	87.973	no
[Ref.: #69480]	gram-positive	yes	88.842	no
[Ref.: #69480]	anaerobic	no	99.139	yes
[Ref.: #69480]	aerobic	yes	91.932	yes
[Ref.: #69480]	halophile	yes	91.94	no
[Ref.: #69480]	spore-forming	no	88.933	no
[Ref.: #69480]	thermophile	no	99.843	no
[Ref.: #69480]	glucose-util	yes	89.251	no
[Ref.: #69480]	motile	no	88.231	no
[Ref.: #69480]	glucose-ferment	yes	85.591	yes
[Ref.: #69480]	flagellated	no	87.973	no
[Ref.: #69480]	gram-positive	yes	88.842	no
[Ref.: #69480]	anaerobic	no	99.139	yes
[Ref.: #69480]	aerobic	yes	91.932	yes
[Ref.: #69480]	halophile	yes	91.94	no
[Ref.: #69480]	spore-forming	no	88.933	no
[Ref.: #69480]	thermophile	no	99.843	no
[Ref.: #69480]	glucose-util	yes	89.251	no
[Ref.: #69480]	motile	no	88.231	no
[Ref.: #69480]	glucose-ferment	yes	85.591	yes

Availability in culture collections External links

[Ref.: #1830]

Culture collection no.

DSM 4807, ATCC 43808, CCUG 25351, NCTC 12218, CCM 4070, CIP 103643

[Ref.: #83698]

SI-ID 7699

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Staphylococcus schleiferi subsp. coagulans subsp. nov., isolated from the external auditory meatus of dogs with external ear otitis.	Igimi S, Takahashi E, Mitsuoka T	Int J Syst Bacteriol	10.1099/00207713-40-4-409	1990	*

References

#1830

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 4807

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#34406 ; Curators of the CIP;
#48178 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 25351

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68375

Automatically annotated from API ID32STA .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83698

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7699.1 )

#122511 Collection of Institut Pasteur ; Curators of the CIP; CIP 103643
* These data were automatically processed and therefore are not curated

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Staphylococcus schleiferi NCTC12218

Staphylococcus schleiferi NCTC12218

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