Geobacillus uzenensis (DSM 23175, VKM B-2229, B-10340)

Name: Geobacillus uzenensis (DSM 23175, VKM B-2229, B-10340)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1446

Type strain

Strain Designation B-10340, U

Culture col. no. DSM 23175 VKM B-2229 B-10340 U

NCBI tax ID(s) 129339

Special Collections DSMZ Literature strains

History <- T. N. Nazina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; U

Links

Geobacillus uzenensis B-10340 is a chemoorganotroph, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from formation waters of the Uzen oil field.

spore-forming Gram-positive motile rod-shaped colony-forming chemoorganotroph 16S sequence Bacteria

Name and taxonomic classification

SI-ID 49486

DSM 23175,DSM 13551,VKM B-2229,CCM 7226,NCIMB 13893,KACC 11368,BCRC 17567,CCRC 17567

@ref 20215

Last LPSN update 2026-02-09 11:18:06

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Geobacillus

Species Geobacillus uzenensis

Full scientific name Geobacillus uzenensis Nazina et al. 2001

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
23044	positive	4.7-8.0 µm	0.9-1.3 µm	rod-shaped		peritrichous

Colony morphology

@ref	Colony size	Colony color	Colony shape
23044	1.0 mm	colourless	circular

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
6276	CASO AGAR (MERCK 105458) (DSMZ Medium 220)		Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220; with strain-specific modifications) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l MnSO4 0.01 g/l Distilled water
6276	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
6276	positive	growth	55
23044	positive	growth	45.0-65.0
23044	positive	optimum	55.0-65.0
23044

Culture pH

@ref	Ability	Type	PH
23044	positive	optimum	6.2-7.5
23044	positive	growth	6.2-7.8

Physiology and metabolism

Nutrition type

23044

Typechemoorganotroph

Spore formation

@ref	Spore description	Type of spore	Spore formation
23044	ellipsoidal,terminal,in swollen or non-swollen sporangia	endospore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23044	NaCl	positive	growth	0.0-4.0 %(w/v)
23044	NaCl	positive	growth	4.0 %(w/v)
23044	NaCl	positive	growth	0.0 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23044	22599 ChEBI	arabinose	-	builds gas from
23044		casein	-	hydrolysis
23044	17057 ChEBI	cellobiose	-	builds gas from
23044	17057 ChEBI	cellobiose	+	builds acid from
23044	16947 ChEBI	citrate	-	carbon source
23044	65327 ChEBI	D-xylose	-	builds acid from
23044	4853 ChEBI	esculin	+	hydrolysis
23044	29034 ChEBI	ferric iron	-	reduction
23044	28757 ChEBI	fructose	-	builds gas from
23044	28757 ChEBI	fructose	+	builds acid from
23044	28260 ChEBI	galactose	-	builds gas from
23044	28260 ChEBI	galactose	+	builds acid from
23044	5291 ChEBI	gelatin	+	hydrolysis
23044	17234 ChEBI	glucose	-	builds gas from
23044	17234 ChEBI	glucose	-	fermentation
23044	17234 ChEBI	glucose	+	builds acid from
23044	17754 ChEBI	glycerol	-	builds gas from
23044	17754 ChEBI	glycerol	+	builds acid from
23044	30849 ChEBI	L-arabinose	+	builds acid from
23044	17716 ChEBI	lactose	-	builds acid from
23044	17306 ChEBI	maltose	-	builds gas from
23044	17306 ChEBI	maltose	+	builds acid from
23044	29864 ChEBI	mannitol	-	builds gas from
23044	29864 ChEBI	mannitol	+	builds acid from
23044	37684 ChEBI	mannose	-	builds gas from
23044	37684 ChEBI	mannose	+	builds acid from
23044	17268 ChEBI	myo-inositol	-	builds acid from
23044	17632 ChEBI	nitrate	+	reduction
23044	16634 ChEBI	raffinose	-	builds acid from
23044	26546 ChEBI	rhamnose	-	builds acid from
23044	15963 ChEBI	ribitol	-	builds acid from
23044	33942 ChEBI	ribose	-	builds gas from
23044	33942 ChEBI	ribose	+	builds acid from
23044	30911 ChEBI	sorbitol	-	builds acid from
23044	28017 ChEBI	starch	+	hydrolysis
23044	17992 ChEBI	sucrose	-	builds gas from
23044	17992 ChEBI	sucrose	+	builds acid from
23044	27082 ChEBI	trehalose	-	builds gas from
23044	27082 ChEBI	trehalose	+	builds acid from
23044	18186 ChEBI	tyrosine	-	degradation
23044	16199 ChEBI	urea	-	degradation

Metabolite production

@ref	Chebi-ID	Metabolite
23044	15688 ChEBI	acetoin
23044	16016 ChEBI	dihydroxyacetone
23044	16136 ChEBI	hydrogen sulfide
23044	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Citrate test	Methylred-test
23044	15688 ChEBI	acetoin	-
23044	16947 ChEBI	citrate		-
23044	17234 ChEBI	glucose			-

Enzymes

@ref	Value	Activity
23044	lecithinase	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Condition	#Thermophilic (>45°C)	-
#Engineered	#Industrial	#Oil reservoir
#Environmental	#Aquatic	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Enrichment culture	Enrichment culture temperature
6276	formation waters of the Uzen oil field	Uzen, Khazakhstan	Kazakhstan	KAZ	Asia
23044	formation waters of high-temperature oilfields					agar medium containing n-hexadecane	55.0-60.0

Taxonmaps

69479

Global distribution of 16S sequence AF276304 (>99% sequence identity) for Geobacillus from Microbeatlas

Aquatic Samples	94
Soil Samples	44
Animal Samples	263
Plant Samples	25
Total Samples	426

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
6276	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
6276	Geobacillus uzenensis strain U (T) 16S ribosomal RNA gene, partial sequence	AF276304	1463		129339

GC content

@ref	GC-content (mol%)	Method
6276	50.4
23044	50.4	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Functional Characterization, Genome Assembly, and Annotation of Geobacillus sp. G4 Isolated from a Geothermal Field in Tacna, Peru.	Ticona ARP, Santos KCR, Ramirez-Arua HE, Castellanos R, Silva JP, Hamann PRV, Noronha EF, Lopes FAC.	Microorganisms	10.3390/microorganisms13061374	2025
	Immobilization of Hyperthermostable Carboxylesterase EstD9 from Anoxybacillus geothermalis D9 onto Polymer Material and Its Physicochemical Properties.	Johan UUM, Rahman RNZRA, Kamarudin NHA, Latip W, Ali MSM.	Polymers (Basel)	10.3390/polym15061361	2023
Enzymology	Isolation and identification of lipase producing thermophilic Geobacillus sp. SBS-4S: cloning and characterization of the lipase.	Tayyab M, Rashid N, Akhtar M.	J Biosci Bioeng	10.1016/j.jbiosc.2010.11.015	2011
Enzymology	Primers for amplification of nitrous oxide reductase genes associated with Firmicutes and Bacteroidetes in organic-compound-rich soils.	Jung J, Choi S, Jung H, Scow KM, Park W.	Microbiology (Reading)	10.1099/mic.0.060194-0	2013
	High Quality Draft Genomes of the Type Strains Geobacillus thermocatenulatus DSM 730(T), G. uzenensis DSM 23175(T) And Parageobacillus galactosidasius DSM 18751(T).	Ramaloko WT, Koen N, Polliack S, Aliyu H, Lebre PH, Mohr T, Oswald F, Zwick M, Zeigler DR, Neumann A, Syldatk C, Cowan DA, De Maayer P	J Genomics	10.7150/jgen.22986	2018
Phylogeny	Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. th.	Nazina TN, Tourova TP, Poltaraus AB, Novikova EV, Grigoryan AA, Ivanova AE, Lysenko AM, Petrunyaka VV, Osipov GA, Belyaev SS, Ivanov MV.	Int J Syst Evol Microbiol	10.1099/00207713-51-2-433	2001
Enzymology	Geobacillus zalihae sp. nov., a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia.	Abd Rahman RN, Leow TC, Salleh AB, Basri M.	BMC Microbiol	10.1186/1471-2180-7-77	2007

References

#6276	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 23175
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23044	T. N. Nazina,T. P. Tourova,A. B. Poltaraus,E. V. Novikova,A. A. Grigoryan,A. E. Ivanova,A. M. Lysenko,V. V. Petrunyaka,G. A. Osipov,S. S. Belyaev,M. V. Ivanov: Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. th.. IJSEM 51: 433 - 446 2001 ( DOI 10.1099/00207713-51-2-433 , PubMed 11321089 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Geobacillus uzenensis (DSM 23175, VKM B-2229, B-10340)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Nutrition type

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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