Name: Macrococcus bovicus C2F4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14421

Type strain:

Species: Macrococcus bovicus

Strain Designation: C2F4, DD4516, DD 4516

Culture col. no.: CCUG 38365, CCM 4655, DSM 15607, ATCC 51825, CIP 105714

Strain history: CIP <- 1998, C.G. George, North Carolina State Univ., Raleigh, NC, USA: strain DD 4516 <- W. Kloos

NCBI tax ID(s): 69968 (species)

SI-ID 43201

ATCC 51825, CIP 105714, CCM 4655, CCUG 38365, DSM 15607

Other strains from Macrococcus bovicus

M. bovicus CIP 105713, ATCC 51827
M. bovicus CIP 105715, ATCC 51826
M. bovicus CCUG 38366, CCM 4656
M. bovicus CCUG 46868

Section

Macrococcus bovicus C2F4 is an obligate aerobe, mesophilic, Gram-positive bacterium that was isolated from udder of Holstein cow.

Gram-positive
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Staphylococcaceae*
	Genus	*Macrococcus*
	Species	**Macrococcus bovicus**
	Full Scientific Name (LPSN)	Macrococcus bovicus Kloos et al. 1998

Information on morphological and physiological properties Morphology

[Ref.: #116905]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #116905]	Cell shape	coccus-shaped

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5926]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #5926]

Culture medium growth

[Ref.: #5926]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5926]

Culture medium composition

expand / minimize

[Ref.: #39344]

Culture medium

MEDIUM 3 - Columbia agar

[Ref.: #39344]

Culture medium growth

[Ref.: #39344]

Culture medium composition

expand / minimize

[Ref.: #116905]

Culture medium

CIP Medium 3

[Ref.: #116905]

Culture medium growth

[Ref.: #116905]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5926]		growth	37 °C
[Ref.: #39344]		growth	30 °C
[Ref.: #116905]		growth	22-41 °C
[Ref.: #116905]	no	growth	10 °C
[Ref.: #116905]	no	growth	45 °C

[Ref.: #5926]	Temperature range	mesophilic
[Ref.: #39344]	Temperature range	mesophilic
[Ref.: #116905]	Temperature range	psychrophilic
[Ref.: #116905]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #116905]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %97
[Ref.: #69480]	Ability of spore formation	no Confidence in %98.989

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #116905]	hippurate ChEBI	+	hydrolysis
[Ref.: #116905]	nitrate ChEBI	-	reduction
[Ref.: #116905]	nitrite ChEBI	-	reduction

	Metabolite production	Metabolite	Production
[Ref.: #116905]		indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #116905]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #116905]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	-	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116905]	amylase	-
[Ref.: #116905]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	-	3.2.1.31	* from API zym
[Ref.: #116905]	caseinase	+	3.4.21.50
[Ref.: #116905]	catalase	+	1.11.1.6
[Ref.: #116905]	coagulase	-
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #116905]	DNase	+/-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #116905]	gamma-glutamyltransferase	-	2.3.2.2
[Ref.: #116905]	gelatinase	+/-
[Ref.: #116905]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	-	3.4.11.1	* from API zym
[Ref.: #116905]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116905]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116905]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116905]	oxidase	+
[Ref.: #116905]	phenylalanine ammonia-lyase	-	4.3.1.24
[Ref.: #116905]	protease	+
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116905]	tryptophan deaminase	-
[Ref.: #116905]	tween esterase	-
[Ref.: #116905]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #116905]	1329	not determinedn.d.	+	+	-	+	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	+	-	-	+	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116905]	8931	-	+	+	+	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5926]	Sample type/isolated from	udder of Holstein cow
[Ref.: #5926]	Geographic location (country and/or sea, region)	North Carolina, Raleigh
[Ref.: #5926]	Country	USA
[Ref.: #5926]	Country ISO 3 Code	USA
[Ref.: #5926]	Continent	North America

[Ref.: #54219]	Sample type/isolated from	Bovine,skin of a Holstein cow

[Ref.: #116905]	Sample type/isolated from	Holstein cow
[Ref.: #116905]	Country	United States of America
[Ref.: #116905]	Country ISO 3 Code	USA
[Ref.: #116905]	Continent	North America
[Ref.: #116905]	Isolation date	1994
* marker position based on {}

Isolation sources categories

#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body-Site	-	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5926]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116905]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #5926]	Macrococcus bovicus 16S rRNA gene	Y15714	1546	ENA	69968 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Macrococcus bovicus ATCC 51825	GCA_004359575	contig	GenBank	69968 tax ID	*
[Ref.: #66792]	Macrococcus bovicus strain ATCC 51825	69968.3	wgs	PATRIC	69968 tax ID	*
[Ref.: #66792]	Macrococcus bovicus ATCC 51825	2895704629	draft	JGI IMG/M	69968 tax ID	*

[Ref.: #5926]

GC-content

44 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Macrococcus bovicus strain ATCC 51825 PATRIC: 69968.3
[Ref.: #69481]	spore-forming	no	97	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Macrococcus bovicus strain ATCC 51825 PATRIC: 69968.3
[Ref.: #69480]	gram-positive	yes	93.338	no
[Ref.: #69480]	anaerobic	no	98.691	no
[Ref.: #69480]	halophile	yes	87.753	no
[Ref.: #69480]	spore-forming	no	84.083	no
[Ref.: #69480]	glucose-util	yes	88.563	no
[Ref.: #69480]	thermophile	no	99.774	no
[Ref.: #69480]	flagellated	no	93.423	no
[Ref.: #69480]	aerobic	yes	85.211	no
[Ref.: #69480]	glucose-ferment	no	74.691	no
[Ref.: #69480]	motile	no	91.65	no

Availability in culture collections External links

[Ref.: #5926]

Culture collection no.

CCUG 38365, CCM 4655, DSM 15607, ATCC 51825, CIP 105714

[Ref.: #83565]

SI-ID 43201

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Draft Genome Sequences of the Type Strains of Six Macrococcus Species.	Mazhar S, Altermann E, Hill C, McAuliffe O	Microbiol Resour Announc	10.1128/MRA.00344-19	2019	*
Phylogeny	Delimiting the genus Staphylococcus through description of Macrococcus caseolyticus gen. nov., comb. nov. and Macrococcus equipercicus sp. nov., and Macrococcus bovicus sp. no. and Macrococcus carouselicus sp. nov.	Kloos WE, Ballard DN, George CG, Webster JA, Hubner RJ, Ludwig W, Schleifer KH, Fiedler F, Schubert K	Int J Syst Bacteriol	10.1099/00207713-48-3-859	1998	*

References

#5926

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 15607

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#39344 ; Curators of the CIP;
#54219 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 38365

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68382

Automatically annotated from API zym .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83565

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID43201.1 )

#116905 Collection of Institut Pasteur ; Curators of the CIP; CIP 105714
* These data were automatically processed and therefore are not curated

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Macrococcus bovicus strain ATCC 51825

Macrococcus bovicus strain ATCC 51825

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