Name: Sporolactobacillus putidus QC81-06
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14405

Type strain:

Species: Sporolactobacillus putidus

Strain Designation: QC81-06

Culture col. no.: DSM 21265, JCM 15325

Strain history: R. Fujita QC81-06.

NCBI tax ID(s): 492735 (species)

SI-ID 397237

DSM 21265

Section

Sporolactobacillus putidus QC81-06 is an anaerobe, spore-forming, mesophilic bacterium that was isolated from spoiled orange juice.

Gram-positive
motile
rod-shaped
spore-forming
anaerobe
mesophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Sporolactobacillaceae*
	Genus	*Sporolactobacillus*
	Species	**Sporolactobacillus putidus**
	Full Scientific Name (LPSN)	Sporolactobacillus putidus Fujita et al. 2010

Information on morphological and physiological properties Morphology

[Ref.: #28936]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #28936]	Cell length	5.5 µm

[Ref.: #28936]	Cell width	0.65 µm

[Ref.: #28936]	Cell shape	rod-shaped

[Ref.: #28936]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %92.99

[Ref.: #28936]

Pigment production

Information on culture and growth conditions Culture and growth conditions

[Ref.: #15496]

Culture medium

GYP GLUCOSE-YEAST-PEPTONE MEDIUM (DSMZ Medium 852)

[Ref.: #15496]

Culture medium growth

[Ref.: #15496]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15496]

Culture medium composition

expand / minimize

[Ref.: #15496]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #15496]

Culture medium growth

[Ref.: #15496]

Culture medium link

Medium recipe at MediaDive

[Ref.: #15496]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #15496]	growth	35 °C
[Ref.: #15496]	growth	30 °C
[Ref.: #28936]	growth	30-45 °C
[Ref.: #28936]	optimum	35 °C
[Ref.: #67770]	growth	35 °C

[Ref.: #15496]	Temperature range	mesophilic
[Ref.: #28936]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #28936]			optimum	4.75
[Ref.: #28936]			growth	4.5-5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #28936]

Oxygen tolerance

anaerobe

[Ref.: #28936]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

[Ref.: #67770]

Observation

quinones: MK-7, MK-3

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #28936]	fructose ChEBI	+	carbon source
[Ref.: #28936]	galactose ChEBI	+	carbon source
[Ref.: #28936]	glucose ChEBI	+	carbon source
[Ref.: #28936]	maltose ChEBI	+	carbon source
[Ref.: #28936]	mannitol ChEBI	+	carbon source
[Ref.: #28936]	mannose ChEBI	+	carbon source
[Ref.: #28936]	N-acetylglucosamine ChEBI	+	carbon source
[Ref.: #28936]	sucrose ChEBI	+	carbon source
[Ref.: #28936]	trehalose ChEBI	+	carbon source

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #15496]	Sample type/isolated from	spoiled orange juice
[Ref.: #15496]	Geographic location (country and/or sea, region)	Shizuoka
[Ref.: #15496]	Country	Japan
[Ref.: #15496]	Country ISO 3 Code	JPN
[Ref.: #15496]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Spoiled orange juice
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Beverage
#Environmental	#Microbial community	-

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #15496]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #15496]	Sporolactobacillus putidus gene for 16S ribosomal RNA, partial sequence	AB374522	1503	ENA	492735 tax ID

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Sporolactobacillus putidus JCM 15325	GCA_014647035	scaffold	GenBank	492735 tax ID	*
[Ref.: #66792]	Sporolactobacillus putidus strain JCM 15325	492735.3	wgs	PATRIC	492735 tax ID	*

[Ref.: #15496]	GC-content	47.5 mol%	high performance liquid chromatography (HPLC)
[Ref.: #28936]	GC-content	47.5 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Sporolactobacillus putidus JCM 15325 NCBI: GCA_014647035.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Sporolactobacillus putidus JCM 15325 NCBI: GCA_014647035.1
[Ref.: #69480]	flagellated	yes	81.76	no
[Ref.: #69480]	gram-positive	yes	84.007	yes
[Ref.: #69480]	anaerobic	no	89.144	yes
[Ref.: #69480]	aerobic	no	87.775	yes
[Ref.: #69480]	halophile	no	69.862	no
[Ref.: #69480]	spore-forming	yes	94.944	yes
[Ref.: #69480]	glucose-util	yes	86.076	yes
[Ref.: #69480]	thermophile	no	96.107	yes
[Ref.: #69480]	motile	yes	89.534	no
[Ref.: #69480]	glucose-ferment	no	69.38	no

Availability in culture collections External links

[Ref.: #15496]

Culture collection no.

DSM 21265, JCM 15325

[Ref.: #83549]

SI-ID 397237

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sporolactobacillus shoreae sp. nov. and Sporolactobacillus spathodeae sp. nov., two spore-forming lactic acid bacteria isolated from tree barks in Thailand.	Thamacharoensuk T, Kitahara M, Ohkuma M, Thongchul N, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijs.0.000084	2015	*
Phylogeny	Sporolactobacillus putidus sp. nov., an endospore-forming lactic acid bacterium isolated from spoiled orange juice.	Fujita R, Mochida K, Kato Y, Goto K	Int J Syst Evol Microbiol	10.1099/ijs.0.002048-0	2009	*

References

#15496

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 21265

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#28936

Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #25375 (see below)

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83549

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397237.1 )

#25375

IJSEM 1499 2010 ( DOI 10.1099/ijs.0.002048-0 , PubMed 19684317 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Sporolactobacillus putidus in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Sporolactobacillus putidus JCM 15325

Sporolactobacillus putidus JCM 15325

Change proposal

Change proposal