Bacillus thuringiensis (CCUG 22499, IAM 11064, ATCC 13367)

Name: Bacillus thuringiensis (CCUG 22499, IAM 11064, ATCC 13367)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 144043

Type strain

Culture col. no. CCUG 22499 IAM 11064 ATCC 13367 NBRC 13866 JCM 20337

NCBI tax ID(s) 1428

Special Collections CCUG JCM

History JCM <-- IAM 11064 <-- ATCC 13367 <-- R. Davis 1328 <-- E. A. Steinhaus B-13.

Links

Bacillus thuringiensis CCUG 22499 is an aerobe bacterium that was isolated from Moth larva.

aerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 35792

ATCC 13367,CCUG 22499,IAM 11064,IFO 13866,NBRC 13866,BCRC 11029,CCRC 11029,CDBB 27,JCM 20337

@ref 20215

Last LPSN update 2026-05-29 08:40:35

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Bacillus

Species Bacillus thuringiensis

Full scientific name Bacillus thuringiensis Berliner 1915 (Approved Lists 1980)

BacDive ID	Other strains from **Bacillus thuringiensis** (90)	Type strain
1006	B. thuringiensis NRS-996, DSM 2046, ATCC 10792, CCM 19, NCIB ... (type strain)
1005	B. thuringiensis 1564, DSM 350
1007	B. thuringiensis DSM 6018, NRRL HD-525
1008	B. thuringiensis DSM 6019, NRRL HD-572
1009	B. thuringiensis DSM 6020, NRRL HD-577
1010	B. thuringiensis DSM 6021, NRRL HD-974
1011	B. thuringiensis DSM 6022, NRRL HD-916
1012	B. thuringiensis DSM 6023, NRRL HD-516
1013	B. thuringiensis DSM 6024, NRRL HD-1NC
1014	B. thuringiensis DSM 6025, NRRL HD-198
1015	B. thuringiensis DSM 6029, NRRL HD-2
1016	B. thuringiensis DSM 6030, NRRL HD-350
1017	B. thuringiensis DSM 6031, NRRL HD-5
1018	B. thuringiensis DSM 6032, NRRL HD-4NC
1019	B. thuringiensis DSM 6033, NRRL HD-500NC
1020	B. thuringiensis DSM 6034, NRRL HD-110
1021	B. thuringiensis NB 31, DSM 5724
1022	B. thuringiensis NB 75, DSM 5725
1023	B. thuringiensis NB221, DSM 5815
1024	B. thuringiensis DSM 6107, NRRL HD-30
1025	B. thuringiensis DSM 6108, NRRL HD-10
1026	B. thuringiensis DSM 6110, NRRL HD-29
1027	B. thuringiensis DSM 6111, NRRL HD-192
1028	B. thuringiensis DSM 6112, NRRL HD-12
1029	B. thuringiensis DSM 6113, NRRL HD-530
1030	B. thuringiensis DSM 6016, NRRL HD-395
1031	B. thuringiensis DSM 6017, NRRL HD-489
1032	B. thuringiensis DSM 6069, NRRL HD-866
1033	B. thuringiensis DSM 6070, NRRL HD-867
1034	B. thuringiensis DSM 6071, NRRL HD-868
1035	B. thuringiensis DSM 6072, NRRL HD-847
1036	B. thuringiensis DSM 6073, NRRL HD-819
1037	B. thuringiensis DSM 6074, NRRL HD-1011
1038	B. thuringiensis DSM 6075, NRRL HD-1012
1039	B. thuringiensis DSM 6076, NRRL HD-976
1040	B. thuringiensis DSM 6078, NRRL HD-501
1041	B. thuringiensis DSM 6079, NRRL HD-541
1042	B. thuringiensis DSM 6080, NRRL HD-571
1043	B. thuringiensis DSM 6085, NRRL HD-100
1044	B. thuringiensis DSM 6053, NRRL HD-62
1045	B. thuringiensis DSM 6054, NRRL HD-511
1046	B. thuringiensis DSM 6055, NRRL HD-932
1047	B. thuringiensis DSM 6086, NRRL HD-224
1048	B. thuringiensis DSM 6087, NRRL HD-500
1049	B. thuringiensis DSM 6088, NRRL HD-833
1050	B. thuringiensis DSM 6090, NRRL HD-770
1051	B. thuringiensis DSM 6091, NRRL HD-146
1052	B. thuringiensis DSM 6092, NRRL HD-498
1053	B. thuringiensis DSM 6093, NRRL HD-13
1054	B. thuringiensis DSM 6094, NRRL HD-285
1055	B. thuringiensis DSM 6095, NRRL HD-201
1056	B. thuringiensis DSM 6096, NRRL HD-540
1057	B. thuringiensis DSM 6097, NRRL HD-3
1058	B. thuringiensis DSM 6098, NRRL HD-526
1059	B. thuringiensis DSM 6099, NRRL HD-11
1060	B. thuringiensis DSM 6100, NRRL HD-282
1061	B. thuringiensis DSM 6101, NRRL HD-73
1062	B. thuringiensis DSM 6102, NRRL HD-1
1063	B. thuringiensis DSM 6103, NRRL HD-7
1064	B. thuringiensis DSM 6104, NRRL HD-226
1065	B. thuringiensis DSM 6105, NRRL HD-4
1066	B. thuringiensis DSM 6106, NRRL HD-930
1067	B. thuringiensis Bt 131, 4A8, DSM 6890, BGSC 4A8
132697	B. thuringiensis Bt GOE1, DSM 104061
132698	B. thuringiensis Bt GOE2, DSM 104062
132699	B. thuringiensis Bt GOE3, DSM 104063
132700	B. thuringiensis Bt GOE4, DSM 104064
132701	B. thuringiensis Bt GOE5, DSM 104065
132702	B. thuringiensis Bt GOE6, DSM 104066
132703	B. thuringiensis Bt GOE7, DSM 104067
135466	B. thuringiensis CIP 53.1
135552	B. thuringiensis CIP 110226
138886	B. thuringiensis CIP 110224
138887	B. thuringiensis CIP 110225
139020	B. thuringiensis CIP 110223
139026	B. thuringiensis CIP 110227
139027	B. thuringiensis CIP 110228
139229	B. thuringiensis NRS-1124, 58.1.1, CIP 53.138, ATCC 19268
139707	B. thuringiensis Bt 18247, MYBT18247, DSM 104068
157308	B. thuringiensis IMI 348918
159981	B. thuringiensis BnBt, DSM 26055
162881	B. thuringiensis JCM 20331, IAM 11056
162908	B. thuringiensis JCM 20387, ATCC 13366, IAM 12078
163591	B. thuringiensis JCM 28784
163649	B. thuringiensis JCM 28844
174184	B. thuringiensis EA 12392, CRBIP3.2721
174185	B. thuringiensis 1884, CRBIP3.6147
174186	B. thuringiensis 367, CRBIP3.705
174187	B. thuringiensis CIP 110666, CIP 200206
174188	B. thuringiensis CRBIP3.2344, BTS 02618A

Morphology

Culture and growth conditions

Culture temp

@ref	Growth	Type	Temperature (°C)
47650	positive	growth	30-37
67770	positive	growth	30

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
47650	aerobe
125439	facultative anaerobe	99.091

Spore formation

@ref	Spore formation	Confidence
125439		94.926

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
68368	27613 ChEBI	amygdalin	-	fermentation	from API 20E
68371	18305 ChEBI	arbutin	-	builds acid from	from API 50CH acid
68368	29016 ChEBI	arginine	-	hydrolysis	from API 20E
68371	17057 ChEBI	cellobiose	-	builds acid from	from API 50CH acid
68368	16947 ChEBI	citrate	-	assimilation	from API 20E
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	-	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68368	17634 ChEBI	D-glucose	-	fermentation	from API 20E
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68379	16899 ChEBI	D-mannitol	-	fermentation	from API Coryne
68371	16899 ChEBI	D-mannitol	-	builds acid from	from API 50CH acid
68368	16899 ChEBI	D-mannitol	-	fermentation	from API 20E
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68379	16988 ChEBI	D-ribose	+	fermentation	from API Coryne
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
68371	16443 ChEBI	D-tagatose	-	builds acid from	from API 50CH acid
68379	65327 ChEBI	D-xylose	-	fermentation	from API Coryne
68371	65327 ChEBI	D-xylose	-	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
68379	4853 ChEBI	esculin	+	hydrolysis	from API Coryne
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
68379	5291 ChEBI	gelatin	+	hydrolysis	from API Coryne
68368	5291 ChEBI	gelatin	+	hydrolysis	from API 20E
68371	28066 ChEBI	gentiobiose	-	builds acid from	from API 50CH acid
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
68371	17754 ChEBI	glycerol	-	builds acid from	from API 50CH acid
68379	28087 ChEBI	glycogen	+	fermentation	from API Coryne
68371	28087 ChEBI	glycogen	+	builds acid from	from API 50CH acid
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68368	30849 ChEBI	L-arabinose	-	fermentation	from API 20E
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68368	62345 ChEBI	L-rhamnose	-	fermentation	from API 20E
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
68379	17716 ChEBI	lactose	-	fermentation	from API Coryne
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
68368	25094 ChEBI	lysine	-	degradation	from API 20E
68379	17306 ChEBI	maltose	+	fermentation	from API Coryne
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
68371	6731 ChEBI	melezitose	-	builds acid from	from API 50CH acid
68371	28053 ChEBI	melibiose	-	builds acid from	from API 50CH acid
68368	28053 ChEBI	melibiose	-	fermentation	from API 20E
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68368	17268 ChEBI	myo-inositol	-	fermentation	from API 20E
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
68379	17632 ChEBI	nitrate	+	reduction	from API Coryne
68368	18257 ChEBI	ornithine	-	degradation	from API 20E
68371	0 ChEBI	Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371	0 ChEBI	Potassium 5-ketogluconate	-	builds acid from	from API 50CH acid
68371	16634 ChEBI	raffinose	-	builds acid from	from API 50CH acid
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
68371	17814 ChEBI	salicin	-	builds acid from	from API 50CH acid
68368	30911 ChEBI	sorbitol	-	fermentation	from API 20E
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
68379	17992 ChEBI	sucrose	+	fermentation	from API Coryne
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
68368	17992 ChEBI	sucrose	-	fermentation	from API 20E
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
68368	27897 ChEBI	tryptophan	-	energy source	from API 20E
68371	32528 ChEBI	turanose	-	builds acid from	from API 50CH acid
68379	16199 ChEBI	urea	-	hydrolysis	from API Coryne
68368	16199 ChEBI	urea	-	hydrolysis	from API 20E
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Metabolite production

@ref	Chebi-ID	Metabolite
68368	15688 ChEBI	acetoin	from API 20E
68368	16136 ChEBI	hydrogen sulfide	from API 20E
68368	35581 ChEBI	indole	from API 20E

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Indole test
68368	15688 ChEBI	acetoin	+		from API 20E
68368	35581 ChEBI	indole		-	from API 20E

Enzymes

@ref	Value	Activity	Ec
68379	alkaline phosphatase	+	3.1.3.1	from API Coryne
68379	alpha-glucosidase	+	3.2.1.20	from API Coryne
68368	arginine dihydrolase	-	3.5.3.6	from API 20E
68379	beta-galactosidase	-	3.2.1.23	from API Coryne
68368	beta-galactosidase	-	3.2.1.23	from API 20E
68379	beta-glucosidase	+	3.2.1.21	from API Coryne
68379	beta-glucuronidase	-	3.2.1.31	from API Coryne
68379	catalase	+	1.11.1.6	from API Coryne
68368	cytochrome oxidase	-	1.9.3.1	from API 20E
68379	gelatinase	+		from API Coryne
68368	gelatinase	+		from API 20E
68368	lysine decarboxylase	-	4.1.1.18	from API 20E
68379	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API Coryne
68368	ornithine decarboxylase	-	4.1.1.17	from API 20E
68379	pyrazinamidase	+	3.5.1.B15	from API Coryne
68379	pyrrolidonyl arylamidase	+	3.4.19.3	from API Coryne
68368	tryptophan deaminase	-	4.1.99.1	from API 20E
68379	urease	-	3.5.1.5	from API Coryne
68368	urease	-	3.5.1.5	from API 20E

API 20E

@ref	ONPG	ADH (Arg)	LDC (Lys)	ODC	CIT	H2S productionH2S	URE	TDA (Trp)	IND	Acetoin production (Voges Proskauer test)VP	GEL	GLU	MAN	INO	Sor	RHA	SAC	MEL	AMY	ARA	OX	Nitrite productionNO2	Reduction to N2N2	MotilityMOB	Growth on MacConkey mediumMAC	OF-O	OF-F
47650	-	-	-	-	-	-	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.	not determinedn.d.

API coryne

@ref	Reduction of nitrateNIT	PYZ	PYRA	PAL	beta GUR	beta GAL	alpha GLU	beta NAG	ESC	URE	GEL	Control fermentationControl	GLU	RIB	XYL	MAN	MAL	LAC	SAC	GLYG	CAT
47650	+	+	+	+	-	-	+	+	+	-	+	-	not determinedn.d.	+	-	-	+	-	+	+	+

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
47650	-	-	-	-	-	+	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	+	-	-	+	-	-	+	-	-	+	+	-	-	-	+	+	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta

Isolation

@ref	Sample type	Sampling date
47650	Moth larva	1988-04-27
67770	Larva of moth

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
124043	ASM2159554v1 assembly for Bacillus thuringiensis B13	complete	GCA_021595545	1428.1744	1428	95.97
124043	ASM3131641v1 assembly for Bacillus thuringiensis ATCC 13367	scaffold	GCA_031316415	1428.2097	1428	0

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
124043	Bacillus thuringiensis gene for 16S rRNA, partial sequence, strain: NBRC 13866.	AB363741	1477		1428

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM2159554v1 assembly for Bacillus thuringiensis B13
NCBI: GCA_021595545

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	gram_stain	BacteriaNetⓘ	positive	68.96	no
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	99.09	no
125439	motility	BacteriaNetⓘ	yes	80.07	no
125439	spore_formation	BacteriaNetⓘ	yes	94.93	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Adaptation of a eukaryote-like ProRS to a prokaryote-like tRNAPro.	Ivanesthi IR, Latifah E, Amrullah LF, Tseng YK, Chuang TH, Pan HC, Yang CS, Liu SY, Wang CC.	Nucleic Acids Res	10.1093/nar/gkae483	2024
Genetics	Multiple cry Genes in Bacillus thuringiensis Strain BTG Suggest a Broad-Spectrum Insecticidal Activity.	Arsov A, Gerginova M, Paunova-Krasteva T, Petrov K, Petrova P.	Int J Mol Sci	10.3390/ijms241311137	2023
	Rapid Capsular Antigen Immunoassay for Diagnosis of Inhalational Anthrax: Preclinical Studies and Evaluation in a Nonhuman Primate Model.	Gates-Hollingsworth MA, Kolton CB, Hoffmaster AR, Meister GT, Moore AE, Green HR, Pogoda JM, Pillai SP, Kozel TR.	mBio	10.1128/mbio.00931-22	2022
	Development of amplified fragment length polymorphism-derived functional strain-specific markers to assess the persistence of 10 bacterial strains in soil microcosms.	Xiang SR, Cook M, Saucier S, Gillespie P, Socha R, Scroggins R, Beaudette LA.	Appl Environ Microbiol	10.1128/aem.00574-10	2010
Enzymology	Comparison of Bacillus thuringiensis with other bacillus species based on enzymoserological examinations of their proteolytic enzymes.	Sandvik O.	Acta Vet Scand	10.1186/bf03547420	1973
	Transduction in Bacillus thuringiensis.	Thorne CB.	Appl Environ Microbiol	10.1128/aem.35.6.1109-1115.1978	1978
Phylogeny	Genome-wide single nucleotide polymorphism typing method for identification of Bacillus anthracis species and strains among B. cereus group species.	Kuroda M, Serizawa M, Okutani A, Sekizuka T, Banno S, Inoue S.	J Clin Microbiol	10.1128/jcm.00137-10	2010
Genetics	The Complete Genome Sequence of Bacillus thuringiensis Strain ATCC 13367 Features a cry-Containing Chromosome.	Shwed PS, Crosthwait J, Weedmark K, Leveque G	Microbiol Resour Announc	10.1128/mra.01227-21	2022
Enzymology	A novel method of producing the key intermediate ASI-2 of ranirestat using a porcine liver esterase (PLE) substitute enzyme.	Yamamura ET, Tsuzaki K, Kita S	Biosci Biotechnol Biochem	10.1080/09168451.2019.1580139	2019
Phylogeny	Identification and application of AFLP-derived genetic markers for quantitative PCR-based tracking of Bacillus and Paenibacillus spp. released in soil.	Providenti MA, Begin M, Hynes S, Lamarche C, Chitty D, Hahn J, Beaudette LA, Scroggins R, Smith ML	Can J Microbiol	10.1139/w09-071	2009

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#47650	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 22499
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68368	Automatically annotated from API 20E .
#68371	Automatically annotated from API 50CH acid .
#68379	Automatically annotated from API Coryne .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Bacillus thuringiensis (CCUG 22499, IAM 11064, ATCC 13367)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API 20E

API coryne

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_021595545

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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