Sporolactobacillus inulinus (DSM 20348, ATCC 15538, JCM 6014)

Name: Sporolactobacillus inulinus (DSM 20348, ATCC 15538, JCM 6014)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14396

Type strain

Strain Designation EU

Culture col. no. DSM 20348 ATCC 15538 JCM 6014 NRIC 1133 EU 18 more

NCBI tax ID(s) 2078

Special Collections DSMZ CCUG JCM KCTC CIP

History <- K. Kitahara, EU NRIC 1133 <-- K. Kitahara. CIP <- 1979, NCIB <- F. Kitahara <- J. Suzuki

Links

Sporolactobacillus inulinus EU is a mesophilic prokaryote that produces lactate and was isolated from chicken feed.

lactate production mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 297026

LMG 11481,ATCC 14897,ATCC 15538,CECT 691,DSM 20348,IFO 13595,JCM 6014,NCIMB 9743,CIP 103279,NCIB 9743,CCUG 41661,CCRC 14647,IAM 12543,VKM B-1597,CCUG34015,NBRC 13595,NRRL B-14021,CCM 7124,BCRC 14647,CCT 0904,HAMBI 2162,KCTC 5032,CIP 103254,CECT 8647

@ref 20215

Last LPSN update 2026-02-09 11:21:24

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Sporolactobacillaceae

Genus Sporolactobacillus

Species Sporolactobacillus inulinus

Full scientific name Sporolactobacillus inulinus (Kitahara and Suzuki 1963) Kitahara and Lai 1967 (Approved Lists 1980)

Synonyms (1)

"Lactobacillus inulinus"

BacDive ID	Other strains from **Sporolactobacillus inulinus** (2)	Type strain
163088	S. inulinus JCM 21112, IAM 14267, NRIC 1134
164772	S. inulinus JCM 3486, IAM 12384

Morphology

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
8752	MRS MEDIUM (DSMZ Medium 11)	Medium recipe at MediaDive	Name: MRS MEDIUM (DSMZ Medium 11) Composition: Glucose 20.0 g/l Casein peptone 10.0 g/l Meat extract 10.0 g/l Na-acetate 5.0 g/l Yeast extract 5.0 g/l (NH4)3 citrate 2.0 g/l K2HPO4 2.0 g/l Tween 80 1.0 g/l MgSO4 x 7 H2O 0.2 g/l MnSO4 x H2O 0.05 g/l Distilled water
34655	MEDIUM 41- for Lactobacillus, Leuconostoc, Weissella, Pediococcus, Sporolactobacillus inulinus		Distilled water make up to (1000.000 ml);Man Rogosa Sharp broth (55.000 g)
120274	CIP Medium 41	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
8752	positive	growth	30	mesophilic
34655	positive	growth	30	mesophilic
67770	positive	growth	30	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	98.4

Compound production

8752

CompoundD lactic acid

Murein

@ref	Murein short key	Type
8752	A31	A1gamma m-Dpm-direct

Observation

67770

Observationquinones: MK-7

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Birds	#Chicken

Isolation

@ref	Sample type
8752	chicken feed
67770	Chicken feed
120274	Other, Chicken feed

Taxonmaps

69479

Global distribution of 16S sequence AB362770 (>99% sequence identity) for Sporolactobacillus from Microbeatlas

Aquatic Samples	33
Soil Samples	33
Animal Samples	140
Plant Samples	37
Total Samples	243

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
8752	1	Risk group (German classification) According to TRBA 466
120274	1	Risk group (French classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM653928v1 assembly for Sporolactobacillus inulinus NBRC 13595	contig	GCA_006539285	2078.4	8066225554	2078	18.75

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Sporolactobacillus inulinus gene for 16S rRNA, partial sequence, strain: NRIC 1133 (= ATCC 15538, = JCM 6014)	AB362770	1545	2078
20218	Sporolactobacillus inulinis 16S ribosomal RNA	M58838	1553	2078
20218	Sporolactobacillus inulinus genes for 16S rRNA, ITS1, 23S rRNA, partial and complete sequence, strain: DSM 20348	AB231792	562	2078
20218	Sporolactobacillus inulinus gene for 16S rRNA, partial sequence	AB006948	276	2078
20218	Sporolactobacillus inulinus gene for 16S rRNA, partial sequence	AB101595	1503	2078
20218	Sporolactobacillus inulinus gene for 16S rRNA, partial sequence, strain: JCM 6014	D16283	1485	2078
20218	Sporolactobacillus inulinus gene for 16S ribosomal RNA, partial sequence	AB374521	1503	2078
20218	Sporolactobacillus inulinus gene for 16S rRNA, partial sequence, strain: NBRC 13595	AB680455	1476	2078

GC content

@ref	GC-content (mol%)	Method
8752	39.3
67770	47
67770	47.3	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Sporolactobacillus inulinus NBRC 13595
NCBI: GCA_006539285

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	81.90	no
125439	motility	BacteriaNetⓘ	yes	81.00	no
125439	gram_stain	BacteriaNetⓘ	variable	84.50	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	98.40	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Sporolactobacillus inulinus strain NBRC 13595
PATRIC: 2078.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	79.58	no
125438	anaerobic	anaerobicⓘ	no	78.81	no
125438	spore-forming	spore-formingⓘ	yes	85.91	no
125438	aerobic	aerobicⓘ	no	81.00	no
125438	thermophilic	thermophileⓘ	no	90.35	no
125438	flagellated	motile2+ⓘ	yes	79.96	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Recent Advances in d-Lactic Acid Production from Renewable Resources: Case Studies on Agro-Industrial Waste Streams.	Alexandri M, Schneider R, Mehlmann K, Venus J.	Food Technol Biotechnol	10.17113/ftb.57.03.19.6023	2019
	Sporolactobacillus-a new functional genus with potential applications.	Guo X, Yu L, Xiao M, Zang X, Zhang C, Narbad A, Chen W, Tian F, Zhai Q.	Curr Res Food Sci	10.1016/j.crfs.2024.100822	2024
	Advances in L-Lactic Acid Production from Lignocellulose Using Genetically Modified Microbial Systems.	Diaz-Orozco L, Moscosa Santillan M, Delgado Portales RE, Rosales-Colunga LM, Leyva-Porras C, Saavedra-Leos Z.	Polymers (Basel)	10.3390/polym17030322	2025
Phylogeny	Probiotic Cocktail Identified by Microbial Network Analysis Inhibits Growth, Virulence Gene Expression, and Host Cell Colonization of Vancomycin-Resistant Enterococci.	Sun WS, Lee YJ, Tsai KN, Ho YH, Fang SB.	Microorganisms	10.3390/microorganisms8060816	2020
	Nucleotide sequence of 5S ribosomal RNAs from spore-forming lactic acid bacteria and Lactobacillus plantarum.	Yanagida F, Hori H, Suzuki K, Kozaki M, Komagata K.	Nucleic Acids Res	10.1093/nar/16.22.10938	1988
Phylogeny	Electrophoretic study of Clostridium species.	Cato EP, Hash DE, Holdeman LV, Moore WE.	J Clin Microbiol	10.1128/jcm.15.4.688-702.1982	1982
	Compilation of 5S rRNA and 5S rRNA gene sequences.	Specht T, Wolters J, Erdmann VA.	Nucleic Acids Res	10.1093/nar/18.suppl.2215	1990
Enzymology	Aldolase of lactic acid bacteria: a case history in the use of an enzyme as an evolutionary marker.	London J, Kline K.	Bacteriol Rev	10.1128/br.37.4.453-478.1973	1973
Enzymology	L-Lactate dehydrogenase from Cyanidioschyzon merolae shows high catalytic efficiency for pyruvate reduction and is inhibited by ATP.	Yamamoto M, Osanai T, Ito S.	Plant Mol Biol	10.1007/s11103-024-01495-0	2024
	Improving the Quality of Ensiling High-Moisture Alfalfa with Peanut Vine in Different Additives: Fermentation, Nutritional Quality, and Microbial Communities.	Jia H, Wu C, Liu Z, Sun Y, He Y, Chen H, Zu X, Wang L, Gao Y, Wang M, Li Q.	Microorganisms	10.3390/microorganisms13102228	2025
	Microbial valorization of solid wastes from a recirculating aquaculture system and the relevant microbial functions	Lee J, Kim IS, Emmanuel A, Koh SC.	Aquacultural engineering.	10.1016/j.aquaeng.2019.102016	2019
Metabolism	Betaine Improves Polymer-Grade D-Lactic Acid Production by Sporolactobacillus inulinus Using Ammonia as Green Neutralizer.	Lv G, Che C, Li L, Xu S, Guan W, Zhao B, Ju J.	Pol J Microbiol	10.5604/01.3001.0010.7880	2017
Metabolism	Steps Toward High-Performance PLA: Economical Production of d-Lactate Enabled by a Newly Isolated Sporolactobacillus terrae Strain.	Han X, Huang K, Tang H, Ni J, Liu J, Xu P, Tao F.	Biotechnol J	10.1002/biot.201800656	2019
Metabolism	Sodium ions activated phosphofructokinase leading to enhanced D-lactic acid production by Sporolactobacillus inulinus using sodium hydroxide as a neutralizing agent.	Zheng L, Liu M, Sun J, Wu B, He B.	Appl Microbiol Biotechnol	10.1007/s00253-017-8120-0	2017
Metabolism	[Characterization of D-lactate dehydrogenase isozymes from a D-lactic acid producing bacterium Sporolactobacillus inulinus].	Zhang D, Zheng L, Wu B, He B.	Wei Sheng Wu Xue Bao		2016
Metabolism	Relative catalytic efficiencies and transcript levels of three d- and two l-lactate dehydrogenases for optically pure d-lactate production in Sporolactobacillus inulinus.	Wu B, Yu Q, Zheng S, Pedroso MM, Guddat LW, He B, Schenk G.	Microbiologyopen	10.1002/mbo3.704	2019
Enzymology	Improvement in the catalytic performance of a phenylpyruvate reductase from Lactobacillus plantarum by site-directed and saturation mutagenesis based on the computer-aided design.	Zhang D, Zhu X, Hu D, Wen Z, Zhang C, Wu M.	3 Biotech	10.1007/s13205-020-02633-3	2021
	High concentrate diets altered the structure and function of rumen microbiome in goats.	Mao J, Wang L, Wang Z, Xue B, Peng Q, Hu R, Xiao J.	Front Microbiol	10.3389/fmicb.2024.1416883	2024
Enzymology	Enzymatic characterization of D-lactate dehydrogenase and application in alanine aminotransferase activity assay kit.	Sun Y, Gao G, Cai T.	Bioengineered	10.1080/21655979.2021.1972781	2021
Metabolism	The D-Lactate Dehydrogenase from Sporolactobacillus inulinus Also Possessing Reversible Deamination Activity.	Zhu L, Xu X, Wang L, Dong H, Yu B.	PLoS One	10.1371/journal.pone.0139066	2015
	Improved hydrogen production from pharmaceutical intermediate wastewater in an anaerobic maifanite-immobilized sludge reactor.	Liu R, Lin Y, Ye X, Hu J, Xu G, Li Y.	RSC Adv	10.1039/d1ra02522h	2021
Metabolism	NADP+-Preferring D-Lactate Dehydrogenase from Sporolactobacillus inulinus.	Zhu L, Xu X, Wang L, Dong H, Yu B, Ma Y.	Appl Environ Microbiol	10.1128/aem.01871-15	2015
Enzymology	D-Lactic Acid Production from Sugarcane Bagasse by Genetically Engineered Saccharomyces cerevisiae.	Sornlek W, Sae-Tang K, Watcharawipas A, Wongwisansri S, Tanapongpipat S, Eurwilaichtr L, Champreda V, Runguphan W, Schaap PJ, Martins Dos Santos VAP.	J Fungi (Basel)	10.3390/jof8080816	2022
Metabolism	Evolving Escherichia coli Host Strains for Efficient Deuterium Labeling of Recombinant Proteins Using Sodium Pyruvate-d₃.	Kelpsas V, Leung A, von Wachenfeldt C.	Int J Mol Sci	10.3390/ijms22189678	2021
	Intestinal microbial composition changes induced by Lactobacillus plantarum GBL 16, 17 fermented feed and intestinal immune homeostasis regulation in pigs.	Yu DY, Oh SH, Kim IS, Kim GI, Kim JA, Moon YS, Jang JC, Lee SS, Jung JH, Park J, Cho KK.	J Anim Sci Technol	10.5187/jast.2022.e89	2022
	Complexation of multiple mineral elements by fermentation and its application in laying hens.	Chen H, Heng X, Li K, Wang Z, Ni Z, Gao E, Yong Y, Wu X.	Front Nutr	10.3389/fnut.2022.1001412	2022
	Non-sterilized fermentation of high optically pure D-lactic acid by a genetically modified thermophilic Bacillus coagulans strain.	Zhang C, Zhou C, Assavasirijinda N, Yu B, Wang L, Ma Y.	Microb Cell Fact	10.1186/s12934-017-0827-1	2017
Metabolism	Production of D-lactic acid by bacterial fermentation of rice starch.	Fukushima K, Sogo K, Miura S, Kimura Y.	Macromol Biosci	10.1002/mabi.200400080	2004
	The Magnesium Concentration in Yeast Extracts Is a Major Determinant Affecting Ethanol Fermentation Performance of Zymomonas mobilis.	Li R, Jin M, Du J, Li M, Chen S, Yang S.	Front Bioeng Biotechnol	10.3389/fbioe.2020.00957	2020
Metabolism	Hydrogen peroxide-forming NADH oxidase belonging to the peroxiredoxin oxidoreductase family: existence and physiological role in bacteria.	Nishiyama Y, Massey V, Takeda K, Kawasaki S, Sato J, Watanabe T, Niimura Y.	J Bacteriol	10.1128/jb.183.8.2431-2438.2001	2001
	Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates.	Goldmanns J, Rohling GA, Lipa MK, Scholand T, Deitert A, May T, Haas EP, Boy M, Herold A, Buchs J.	BMC Biotechnol	10.1186/s12896-023-00793-7	2023
	Nitric oxide increases biofilm formation in Saccharomyces cerevisiae by activating the transcriptional factor Mac1p and thereby regulating the transmembrane protein Ctr1.	Yang L, Zheng C, Chen Y, Shi X, Ying Z, Ying H.	Biotechnol Biofuels	10.1186/s13068-019-1359-1	2019
Enzymology	Crystallization and preliminary crystallographic analysis of a flavoprotein NADH oxidase from Lactobacillus brevis.	Kuzu M, Niefind K, Hummel W, Schomburg D.	Acta Crystallogr Sect F Struct Biol Cryst Commun	10.1107/s174430910501153x	2005
Metabolism	Structure-function analyses of alkylhydroperoxidase D from Streptococcus pneumoniae reveal an unusual three-cysteine active site architecture.	Meng Y, Sheen CR, Magon NJ, Hampton MB, Dobson RCJ.	J Biol Chem	10.1074/jbc.ra119.012226	2020
Phylogeny	Phylogenetic relationships between Bacillus species and related genera inferred from 16s rDNA sequences.	Wei Wang MS.	Braz J Microbiol	10.1590/s1517-838220090003000013	2009
	Nucleic acids of species of Lactobacillus.	Sriranganathan N, Seidler RJ, Sandine WE.	J Dairy Sci	10.3168/jds.s0022-0302(85)80932-2	1985
Biotechnology	The use of the carbon/nitrogen ratio and specific organic loading rate as tools for improving biohydrogen production in fixed-bed reactors.	Anzola-Rojas MDP, Goncalves da Fonseca S, Canedo da Silva C, Maia de Oliveira V, Zaiat M.	Biotechnol Rep (Amst)	10.1016/j.btre.2014.10.010	2015
	Lipoquinones of some spore-forming rods, lactic-acid bacteria and actinomycetes.	Hess A, Hollander R, Mannheim W.	J Gen Microbiol	10.1099/00221287-115-1-247	1979
	Branched chain fatty acids are constituents of the normal healthy newborn gastrointestinal tract.	Ran-Ressler RR, Devapatla S, Lawrence P, Brenna JT.	Pediatr Res	10.1203/pdr.0b013e318184d2e6	2008
Enzymology	Extracellular nuclease in the genus Lactobacillus.	Miller A, Sandine WE, Elliker PR.	J Bacteriol	10.1128/jb.108.1.604-606.1971	1971
Metabolism	A hydrogen peroxide-forming NADH oxidase that functions as an alkyl hydroperoxide reductase in Amphibacillus xylanus.	Niimura Y, Nishiyama Y, Saito D, Tsuji H, Hidaka M, Miyaji T, Watanabe T, Massey V.	J Bacteriol	10.1128/jb.182.18.5046-5051.2000	2000
Cultivation	Enhanced production of optically pure d (-) lactic acid from nutritionally rich Borassus flabellifer sugar and whey protein hydrolysate based-fermentation medium.	Reddy Tadi SR, E V R A, Limaye AM, Sivaprakasam S	Biotechnol Appl Biochem	10.1002/bab.1470	2016
Biotechnology	Strain improvement of Sporolactobacillus inulinus ATCC 15538 for acid tolerance and production of D-lactic acid by genome shuffling.	Zheng H, Gong J, Chen T, Chen X, Zhao X	Appl Microbiol Biotechnol	10.1007/s00253-009-2243-x	2009
Pathogenicity	Basic characteristics of Sporolactobacillus inulinus BCRC 14647 for potential probiotic properties.	Huang HY, Huang SY, Chen PY, King VA, Lin YP, Tsen JH	Curr Microbiol	10.1007/s00284-006-0496-5	2007
Metabolism	Biological characterization of D-lactate dehydrogenase responsible for high-yield production of D-phenyllactic acid in Sporolactobacillus inulinus.	Cheng YY, Park TH, Seong H, Kim TJ, Han NS	Microb Biotechnol	10.1111/1751-7915.14125	2022
	Sporolactobacillus mangiferae sp. nov., a spore-forming lactic acid bacterium isolated from tree bark in Thailand.	Phuengjayaem S, Poothong S, Sitdhipol J, Chaiyawan N, Thitiprasert S, Thongchul N, Tanasupawat S.	Int J Syst Evol Microbiol	10.1099/ijsem.0.005993	2023
Phylogeny	Sporolactobacillus vineae sp. nov., a spore-forming lactic acid bacterium isolated from vineyard soil.	Chang YH, Jung MY, Park IS, Oh HM.	Int J Syst Evol Microbiol	10.1099/ijs.0.65608-0	2008
Phylogeny	Scopulibacillus daqui sp. nov., a thermophilic bacterium isolated from high temperature daqu.	Yao S, Zhai L, Xin C, Liu Y, Xu L, Zhang X, Zhao T, Zhang L, Cheng C	Int J Syst Evol Microbiol	10.1099/ijsem.0.001417	2016
Phylogeny	Terrilactibacillus laevilacticus gen. nov., sp. nov., isolated from soil.	Prasirtsak B, Thongchul N, Tolieng V, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.000954	2016

References

#8752	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 20348
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#34655	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120274	Collection of Institut Pasteur ; Curators of the CIP; CIP 103279
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Sporolactobacillus inulinus (DSM 20348, ATCC 15538, JCM 6014)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Compound production

Murein

Observation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_006539285

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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