Geobacillus vulcani (DSM 13174, CIP 106305, 3S-1)

Name: Geobacillus vulcani (DSM 13174, CIP 106305, 3S-1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1439

Type strain

Strain Designation 3S-1

Culture col. no. DSM 13174 CIP 106305 3S-1 KCTC 3847 JCM 12214

NCBI tax ID(s) 135517

Special Collections DSMZ JCM KCTC CIP Literature strains

History <- D. Caccamo, Univ. Messina, Italy; 3S-1 CIP 106305 <-- T. Maugeri 3s-1. CIP <- 1999, T. Maugeri, Messina Univ., Sicily, Italie: strain 3s-1

Links

Geobacillus vulcani 3S-1 is an aerobe, spore-forming, thermophilic prokaryote that was isolated from marine sediment.

spore-forming Gram-positive motile rod-shaped aerobe thermophilic 16S sequence

Name and taxonomic classification

SI-ID 49428

DSM 13174,CIP 106305,LMG19852,KCTC 3847,JCM 12214,KACC 11367,BCRC 17563,CCRC 17563

@ref 20215

Last LPSN update 2026-02-09 11:18:06

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Geobacillus

Species Geobacillus vulcani

Full scientific name Geobacillus vulcani (Caccamo et al. 2000) Nazina et al. 2004

Synonyms (1)

Bacillus vulcani

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
23039	positive	4.0-7.0 µm	0.6-0.8 µm	rod-shaped
123887	positive			rod-shaped

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4958	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)	Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water
40287	Marine agar (MA)		Distilled water make up to (1000.000 ml);Marine agar (55.100 g)
123887	CIP Medium 13	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
4958	positive	growth	50	thermophilic
23039	positive	growth	37.0-72.0
23039	negative	growth	75.0
23039	positive	optimum	60.0	thermophilic
23039				thermophilic
40287	positive	growth	55	thermophilic
67770	positive	growth	55	thermophilic
123887	positive	growth	45-55	thermophilic
123887	negative	growth	10
123887	negative	growth	22
123887	negative	growth	30
123887	negative	growth	37

Culture pH

@ref	Ability	Type	PH	PH range
23039	positive	optimum	6.0
23039	positive	growth	5.5-9.0	alkaliphile
123887		growth	6

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
23105	aerobe
123887	obligate aerobe

Spore formation

@ref	Spore description	Type of spore	Spore formation
23105	oval,terminal	endospore
123887

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23105	NaCl		optimum	2.0 %
23105	NaCl	positive	growth	0.0-3.0 %
23105	NaCl	positive	growth	3.0 %
23105	NaCl		growth	5.0 %
123887	NaCl	positive	growth	0-2 %
123887	NaCl		growth	4 %
123887	NaCl		growth	6 %
123887	NaCl		growth	8 %
123887	NaCl		growth	10 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23105	17128 ChEBI	adipate	+	carbon source
23105	27613 ChEBI	amygdalin	+	builds acid from
68371	27613 ChEBI	amygdalin	-	builds acid from	from API 50CH acid
23105	18305 ChEBI	arbutin	+	builds acid from
68371	18305 ChEBI	arbutin	+	builds acid from	from API 50CH acid
23105		casein	-	hydrolysis
23105	17057 ChEBI	cellobiose	+	builds acid from
23105	17057 ChEBI	cellobiose	+	carbon source
68371	17057 ChEBI	cellobiose	+	builds acid from	from API 50CH acid
23105	16947 ChEBI	citrate	+	carbon source
123887	16947 ChEBI	citrate	-	carbon source
68371	17108 ChEBI	D-arabinose	-	builds acid from	from API 50CH acid
68371	18333 ChEBI	D-arabitol	-	builds acid from	from API 50CH acid
68371	15824 ChEBI	D-fructose	+	builds acid from	from API 50CH acid
68371	28847 ChEBI	D-fucose	-	builds acid from	from API 50CH acid
68371	12936 ChEBI	D-galactose	+	builds acid from	from API 50CH acid
68371	17634 ChEBI	D-glucose	+	builds acid from	from API 50CH acid
68371	62318 ChEBI	D-lyxose	-	builds acid from	from API 50CH acid
68371	16899 ChEBI	D-mannitol	+	builds acid from	from API 50CH acid
68371	16024 ChEBI	D-mannose	+	builds acid from	from API 50CH acid
68371	16988 ChEBI	D-ribose	+	builds acid from	from API 50CH acid
68371	17924 ChEBI	D-sorbitol	-	builds acid from	from API 50CH acid
23105	65327 ChEBI	D-xylose	+	builds acid from
68371	65327 ChEBI	D-xylose	+	builds acid from	from API 50CH acid
68371	17113 ChEBI	erythritol	-	builds acid from	from API 50CH acid
23105	4853 ChEBI	esculin	+	builds acid from
23105	4853 ChEBI	esculin	+	hydrolysis
123887	4853 ChEBI	esculin	+	hydrolysis
68371	4853 ChEBI	esculin	+	builds acid from	from API 50CH acid
23105	28757 ChEBI	fructose	+	builds acid from
23105	28757 ChEBI	fructose	+	carbon source
68371	16813 ChEBI	galactitol	-	builds acid from	from API 50CH acid
23105	28260 ChEBI	galactose	+	builds acid from
23105	28260 ChEBI	galactose	+	carbon source
23105	5291 ChEBI	gelatin	+	hydrolysis
23105	28066 ChEBI	gentiobiose	+	builds acid from
68371	28066 ChEBI	gentiobiose	+	builds acid from	from API 50CH acid
23105	24265 ChEBI	gluconate	+	builds acid from
23105	24265 ChEBI	gluconate	+	carbon source
68371	24265 ChEBI	gluconate	-	builds acid from	from API 50CH acid
23039	17234 ChEBI	glucose	-	builds gas from
23039	17234 ChEBI	glucose	-	fermentation
23105	17234 ChEBI	glucose	+	builds acid from
23105	17234 ChEBI	glucose	+	carbon source
23105	17754 ChEBI	glycerol	+	builds acid from
68371	17754 ChEBI	glycerol	+	builds acid from	from API 50CH acid
23105	28087 ChEBI	glycogen	+	builds acid from
68371	28087 ChEBI	glycogen	+	builds acid from	from API 50CH acid
123887	606565 ChEBI	hippurate	-	hydrolysis
68371	15443 ChEBI	inulin	-	builds acid from	from API 50CH acid
23039	30849 ChEBI	L-arabinose	-	builds acid from
68371	30849 ChEBI	L-arabinose	-	builds acid from	from API 50CH acid
68371	18403 ChEBI	L-arabitol	-	builds acid from	from API 50CH acid
68371	18287 ChEBI	L-fucose	-	builds acid from	from API 50CH acid
68371	62345 ChEBI	L-rhamnose	-	builds acid from	from API 50CH acid
68371	17266 ChEBI	L-sorbose	-	builds acid from	from API 50CH acid
68371	65328 ChEBI	L-xylose	-	builds acid from	from API 50CH acid
23105	17716 ChEBI	lactose	+	builds acid from
23105	17716 ChEBI	lactose	+	carbon source
68371	17716 ChEBI	lactose	-	builds acid from	from API 50CH acid
23105	25115 ChEBI	malate	+	carbon source
23105	17306 ChEBI	maltose	+	builds acid from
23105	17306 ChEBI	maltose	+	carbon source
68371	17306 ChEBI	maltose	+	builds acid from	from API 50CH acid
23105	29864 ChEBI	mannitol	-	builds acid from
23105	29864 ChEBI	mannitol	+	carbon source
23105	37684 ChEBI	mannose	+	builds acid from
23105	37684 ChEBI	mannose	+	carbon source
68371	6731 ChEBI	melezitose	+	builds acid from	from API 50CH acid
23105	28053 ChEBI	melibiose	+	builds acid from
68371	28053 ChEBI	melibiose	+	builds acid from	from API 50CH acid
68371	320061 ChEBI	methyl alpha-D-glucopyranoside	-	builds acid from	from API 50CH acid
68371	43943 ChEBI	methyl alpha-D-mannoside	-	builds acid from	from API 50CH acid
68371	74863 ChEBI	methyl beta-D-xylopyranoside	-	builds acid from	from API 50CH acid
23039	17268 ChEBI	myo-inositol	-	builds acid from
68371	17268 ChEBI	myo-inositol	-	builds acid from	from API 50CH acid
68371	59640 ChEBI	N-acetylglucosamine	+	builds acid from	from API 50CH acid
23039	17632 ChEBI	nitrate	-	builds gas from
23105	17632 ChEBI	nitrate	-	reduction
123887	17632 ChEBI	nitrate	+	reduction
123887	16301 ChEBI	nitrite	+	reduction
23105	18401 ChEBI	phenylacetate	+	carbon source
68371		Potassium 2-ketogluconate	-	builds acid from	from API 50CH acid
68371		Potassium 5-ketogluconate	+	builds acid from	from API 50CH acid
23105	16634 ChEBI	raffinose	+	builds acid from
68371	16634 ChEBI	raffinose	+	builds acid from	from API 50CH acid
23039	26546 ChEBI	rhamnose	-	builds acid from
23039	15963 ChEBI	ribitol	-	builds acid from
68371	15963 ChEBI	ribitol	-	builds acid from	from API 50CH acid
23105	33942 ChEBI	ribose	+	builds acid from
23105	17814 ChEBI	salicin	+	builds acid from
68371	17814 ChEBI	salicin	+	builds acid from	from API 50CH acid
23039	30911 ChEBI	sorbitol	-	builds acid from
23105	27922 ChEBI	sorbose	-	builds acid from
23105	28017 ChEBI	starch	+	builds acid from
23105	28017 ChEBI	starch	+	hydrolysis
68371	28017 ChEBI	starch	+	builds acid from	from API 50CH acid
23105	17992 ChEBI	sucrose	+	builds acid from
23105	17992 ChEBI	sucrose	+	carbon source
68371	17992 ChEBI	sucrose	+	builds acid from	from API 50CH acid
23105	27082 ChEBI	trehalose	+	builds acid from
23105	27082 ChEBI	trehalose	+	carbon source
68371	27082 ChEBI	trehalose	+	builds acid from	from API 50CH acid
23105	32528 ChEBI	turanose	+	builds acid from
68371	32528 ChEBI	turanose	+	builds acid from	from API 50CH acid
23105	53424 ChEBI	tween 20	+	hydrolysis
23105	53426 ChEBI	tween 80	-	hydrolysis
23105	16199 ChEBI	urea	-	hydrolysis
68371	17151 ChEBI	xylitol	-	builds acid from	from API 50CH acid

Antibiotic resistance

@ref	ChEBI	Metabolite	Is resistant
23105	8309	polymyxin b

Metabolite production

@ref	Chebi-ID	Metabolite
23039	15688 ChEBI	acetoin
23105	15688 ChEBI	acetoin
23105	16136 ChEBI	hydrogen sulfide
23105	35581 ChEBI	indole
123887	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
23039	15688 ChEBI	acetoin	-
123887	15688 ChEBI	acetoin	-
23039	17234 ChEBI	glucose		-
123887	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
123887	alcohol dehydrogenase	-	1.1.1.1
23039	alkaline phosphatase	-	3.1.3.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	+	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	+	3.2.1.22	from API zym
23039	alpha-glucosidase	-	3.2.1.20
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
123887	amylase	+
68382	beta-galactosidase	-	3.2.1.23	from API zym
123887	beta-galactosidase	-	3.2.1.23
68382	beta-glucosidase	-	3.2.1.21	from API zym
68382	beta-glucuronidase	-	3.2.1.31	from API zym
123887	caseinase	-	3.4.21.50
23039	catalase	+	1.11.1.6
123887	catalase	-	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
23039	cytochrome oxidase	-	1.9.3.1
123887	DNase	-
23039	esterase (C 4)	-
68382	esterase (C 4)	-		from API zym
23039	esterase lipase (C 8)	-
68382	esterase lipase (C 8)	+		from API zym
123887	gamma-glutamyltransferase	-	2.3.2.2
123887	gelatinase	-
123887	lecithinase	-
68382	leucine arylamidase	+	3.4.11.1	from API zym
123887	lipase	-
68382	lipase (C 14)	-		from API zym
123887	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
123887	ornithine decarboxylase	-	4.1.1.17
123887	oxidase	+
123887	protease	-
68382	trypsin	-	3.4.21.4	from API zym
123887	tween esterase	-
23039	urease	-	3.5.1.5
123887	urease	-	3.5.1.5
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
123887	-	+	-	+	-	+	-	-	-	+	+	+	+	-	-	+	-	-	-	-

API 50CHac

@ref	ControlQ	GLY	ERY	DARA	LARA	RIB	DXYL	LXYL	ADO	MDX	GAL	GLU	FRU	MNE	SBE	RHA	DUL	INO	MAN	SOR	MDM	MDG	NAG	AMY	ARB	ESC	SAL	CEL	MAL	LAC	MEL	SAC	TRE	INU	MLZ	RAF	AMD	GLYG	XLT	GEN	TUR	LYX	TAG	DFUC	LFUC	DARL	LARL	GNT	2KG	5KG
123887	not determinedn.d.	+	-	-	-	+	+	-	-	-	+	+	+	+	-	-	-	-	+	-	-	-	+	-	+	+	+	+	+	-	+	+	+	-	+	+	+	+	-	+	+	-	+/-	-	-	-	-	-	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine
#Environmental	#Aquatic	#Sediment

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date	Latitude	Longitude	Enrichment culture	Enrichment culture duration	Enrichment culture temperature
4958	marine sediment	Eolian Islands 14°58' E, 38°25' N, Mediterranean Sea, Volcano-La Roya					38.4167	14.9667 38.4167/14.9667
23105	shallow marine hydrothermal vent								Bacto Marine Broth 2216 (Difco) and Medium D	3 days	65
67770	Sediment of a shallow hydrothermal vent at Vulcano Island		Italy	ITA	Europe
123887	Environment, Environmental strain	Sicily	Italy	ITA	Europe	1996

Taxonmaps

69479

Global distribution of 16S sequence AJ293805 (>99% sequence identity) for Geobacillus from Microbeatlas

Aquatic Samples	635
Soil Samples	485
Animal Samples	2412
Plant Samples	182
Total Samples	3714

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4958	1	Risk group (German classification) According to TRBA 466
123887	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Geobacillus vulcani strain BCRC 17563 16S ribosomal RNA gene, partial sequence	EU484349	1532	135517
20218	Geobacillus vulcani strain DSM 13174 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequence	EU157958	516	135517
23105	Bacillus vulcani 16S rRNA gene, strain 3S-1	AJ293805	1532	135517

GC content

@ref	GC-content (mol%)	Method
4958	53
23039	53.0	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov.	Nazina TN, Lebedeva EV, Poltaraus AB, Tourova TP, Grigoryan AA, Sokolova DS, Lysenko AM, Osipov GA	Int J Syst Evol Microbiol	10.1099/ijs.0.02932-0	2004
Phylogeny	Bacillus vulcani sp. nov., a novel thermophilic species isolated from a shallow marine hydrothermal vent.	Caccamo D, Gugliandolo C, Stackebrandt E, Maugeri TL	Int J Syst Evol Microbiol	10.1099/00207713-50-6-2009	2000

References

#4958	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13174
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23039	Tamara N. Nazina,Elena V. Lebedeva,Andrei B. Poltaraus,Tatyana P. Tourova,Alexandre A. Grigoryan,Diyana Sh. Sokolova,Anatoliy M. Lysenko,George A. Osipov: Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov.. IJSEM 54: 2019 - 2024 2004 ( DOI 10.1099/ijs.0.02932-0 , PubMed 15545427 )
#23105	D. Caccamo,C. Gugliandolo,E. Stackebrandt,T. L. Maugeri: Bacillus vulcani sp. nov., a novel thermophilic species isolated from a shallow marine hydrothermal vent.. IJSEM 50: 2009 - 2012 2000 ( DOI 10.1099/00207713-50-6-2009 , PubMed 11155974 )
#40287	; Curators of the CIP;
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#68371	Automatically annotated from API 50CH acid .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#123887	Collection of Institut Pasteur ; Curators of the CIP; CIP 106305
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Geobacillus vulcani (DSM 13174, CIP 106305, 3S-1)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

API zym

API 50CHac

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

Help

Social Media