Geobacillus kaustophilus BD 53 is an aerobe, chemoorganotroph, spore-forming prokaryote that was isolated from pasteurized milk.
spore-forming Gram-positive rod-shaped aerobe chemoorganotroph thermophilic genome sequence 16S sequence| @ref 20215 |
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| Domain Bacteria |
| Phylum Bacillota |
| Class Bacilli |
| Order Caryophanales |
| Family Bacillaceae |
| Genus Geobacillus |
| Species Geobacillus kaustophilus |
| Full scientific name Geobacillus kaustophilus (Priest et al. 1989 ex Prickett 1928) Nazina et al. 2001 |
| Synonyms (1) |
| BacDive ID | Other strains from Geobacillus kaustophilus (4) | Type strain |
|---|---|---|
| 161281 | G. kaustophilus JCM 12893 | |
| 163961 | G. kaustophilus JCM 31151 | |
| 163962 | G. kaustophilus JCM 31152 | |
| 163963 | G. kaustophilus JCM 31153 |
| @ref | Name | Growth | Medium link | Composition | |
|---|---|---|---|---|---|
| 3082 | NUTRIENT AGAR (DSMZ Medium 1) | Medium recipe at MediaDive | Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water | ||
| 38651 | MEDIUM 72- for trypto casein soja agar | Distilled water make up to (1000.000 ml);Trypto casein soy agar (40.000 g) | |||
| 120393 | CIP Medium 72 | Medium recipe at CIP |
| @ref | Growth | Type | Temperature (°C) | Range | |
|---|---|---|---|---|---|
| 3082 | positive | growth | 55 | thermophilic | |
| 23044 | positive | growth | 37.0-75.0 | ||
| 23044 | positive | optimum | 55.0-65.0 | thermophilic | |
| 23044 | thermophilic | ||||
| 38651 | positive | growth | 55 | thermophilic | |
| 49445 | positive | growth | 56 | thermophilic | |
| 67770 | positive | growth | 55 | thermophilic | |
| 120393 | positive | growth | 45-55 | thermophilic | |
| 120393 | negative | growth | 10 | ||
| 120393 | negative | growth | 22 | ||
| 120393 | negative | growth | 30 | ||
| 120393 | negative | growth | 37 |
| 23044 | Typechemoorganotroph |
| @ref | Salt | Growth | Tested relation | Concentration | |
|---|---|---|---|---|---|
| 120393 | NaCl | positive | growth | 0-10 % |
| @ref | Chebi-ID | Metabolite | Utilization activity | Kind of utilization tested | |
|---|---|---|---|---|---|
| 68371 | 27613 ChEBI | amygdalin | - | builds acid from | from API 50CH acid |
| 68371 | 18305 ChEBI | arbutin | + | builds acid from | from API 50CH acid |
| 120393 | 16947 ChEBI | citrate | - | carbon source | |
| 68371 | 17108 ChEBI | D-arabinose | - | builds acid from | from API 50CH acid |
| 68371 | 18333 ChEBI | D-arabitol | - | builds acid from | from API 50CH acid |
| 68371 | 15824 ChEBI | D-fructose | + | builds acid from | from API 50CH acid |
| 68371 | 28847 ChEBI | D-fucose | - | builds acid from | from API 50CH acid |
| 68371 | 17634 ChEBI | D-glucose | + | builds acid from | from API 50CH acid |
| 68371 | 62318 ChEBI | D-lyxose | - | builds acid from | from API 50CH acid |
| 68371 | 16899 ChEBI | D-mannitol | + | builds acid from | from API 50CH acid |
| 68371 | 16024 ChEBI | D-mannose | + | builds acid from | from API 50CH acid |
| 68371 | 17924 ChEBI | D-sorbitol | - | builds acid from | from API 50CH acid |
| 68371 | 16443 ChEBI | D-tagatose | - | builds acid from | from API 50CH acid |
| 68371 | 65327 ChEBI | D-xylose | + | builds acid from | from API 50CH acid |
| 68371 | 17113 ChEBI | erythritol | - | builds acid from | from API 50CH acid |
| 120393 | 4853 ChEBI | esculin | + | hydrolysis | |
| 68371 | 4853 ChEBI | esculin | + | builds acid from | from API 50CH acid |
| 23044 | 28757 ChEBI | fructose | - | builds gas from | |
| 23044 | 28757 ChEBI | fructose | + | builds acid from | |
| 68371 | 16813 ChEBI | galactitol | - | builds acid from | from API 50CH acid |
| 68371 | 28066 ChEBI | gentiobiose | - | builds acid from | from API 50CH acid |
| 23044 | 17234 ChEBI | glucose | - | builds gas from | |
| 23044 | 17234 ChEBI | glucose | + | builds acid from | |
| 68371 | 17754 ChEBI | glycerol | - | builds acid from | from API 50CH acid |
| 68371 | 28087 ChEBI | glycogen | + | builds acid from | from API 50CH acid |
| 120393 | 606565 ChEBI | hippurate | + | hydrolysis | |
| 68371 | 15443 ChEBI | inulin | - | builds acid from | from API 50CH acid |
| 68371 | 30849 ChEBI | L-arabinose | - | builds acid from | from API 50CH acid |
| 68371 | 18403 ChEBI | L-arabitol | - | builds acid from | from API 50CH acid |
| 68371 | 18287 ChEBI | L-fucose | - | builds acid from | from API 50CH acid |
| 68371 | 62345 ChEBI | L-rhamnose | - | builds acid from | from API 50CH acid |
| 68371 | 17266 ChEBI | L-sorbose | - | builds acid from | from API 50CH acid |
| 68371 | 65328 ChEBI | L-xylose | - | builds acid from | from API 50CH acid |
| 23044 | 17306 ChEBI | maltose | - | builds gas from | |
| 23044 | 17306 ChEBI | maltose | + | builds acid from | |
| 68371 | 17306 ChEBI | maltose | + | builds acid from | from API 50CH acid |
| 23044 | 37684 ChEBI | mannose | - | builds gas from | |
| 23044 | 37684 ChEBI | mannose | + | builds acid from | |
| 68371 | 43943 ChEBI | methyl alpha-D-mannoside | - | builds acid from | from API 50CH acid |
| 68371 | 74863 ChEBI | methyl beta-D-xylopyranoside | - | builds acid from | from API 50CH acid |
| 68371 | 17268 ChEBI | myo-inositol | - | builds acid from | from API 50CH acid |
| 68371 | 59640 ChEBI | N-acetylglucosamine | + | builds acid from | from API 50CH acid |
| 120393 | 17632 ChEBI | nitrate | + | reduction | |
| 120393 | 17632 ChEBI | nitrate | - | respiration | |
| 120393 | 16301 ChEBI | nitrite | - | reduction | |
| 68371 | Potassium 2-ketogluconate | - | builds acid from | from API 50CH acid | |
| 68371 | Potassium 5-ketogluconate | - | builds acid from | from API 50CH acid | |
| 68371 | 16634 ChEBI | raffinose | - | builds acid from | from API 50CH acid |
| 68371 | 15963 ChEBI | ribitol | - | builds acid from | from API 50CH acid |
| 68371 | 17814 ChEBI | salicin | + | builds acid from | from API 50CH acid |
| 68371 | 28017 ChEBI | starch | + | builds acid from | from API 50CH acid |
| 23044 | 17992 ChEBI | sucrose | - | builds gas from | |
| 23044 | 17992 ChEBI | sucrose | + | builds acid from | |
| 68371 | 17992 ChEBI | sucrose | - | builds acid from | from API 50CH acid |
| 68371 | 27082 ChEBI | trehalose | + | builds acid from | from API 50CH acid |
| 23044 | 18186 ChEBI | tyrosine | - | degradation | |
| 68371 | 17151 ChEBI | xylitol | - | builds acid from | from API 50CH acid |
| @ref | Value | Activity | Ec | |
|---|---|---|---|---|
| 68382 | acid phosphatase | + | 3.1.3.2 | from API zym |
| 120393 | alcohol dehydrogenase | - | 1.1.1.1 | |
| 68382 | alkaline phosphatase | - | 3.1.3.1 | from API zym |
| 68382 | alpha-chymotrypsin | + | 3.4.21.1 | from API zym |
| 68382 | alpha-fucosidase | - | 3.2.1.51 | from API zym |
| 68382 | alpha-galactosidase | + | 3.2.1.22 | from API zym |
| 68382 | alpha-glucosidase | + | 3.2.1.20 | from API zym |
| 68382 | alpha-mannosidase | - | 3.2.1.24 | from API zym |
| 68382 | beta-galactosidase | + | 3.2.1.23 | from API zym |
| 120393 | beta-galactosidase | + | 3.2.1.23 | |
| 68382 | beta-glucosidase | - | 3.2.1.21 | from API zym |
| 68382 | beta-glucuronidase | - | 3.2.1.31 | from API zym |
| 120393 | catalase | + | 1.11.1.6 | |
| 68382 | cystine arylamidase | - | 3.4.11.3 | from API zym |
| 68382 | esterase (C 4) | + | from API zym | |
| 68382 | esterase lipase (C 8) | + | from API zym | |
| 120393 | gamma-glutamyltransferase | - | 2.3.2.2 | |
| 68382 | leucine arylamidase | - | 3.4.11.1 | from API zym |
| 68382 | lipase (C 14) | - | from API zym | |
| 120393 | lysine decarboxylase | - | 4.1.1.18 | |
| 68382 | N-acetyl-beta-glucosaminidase | - | 3.2.1.52 | from API zym |
| 68382 | naphthol-AS-BI-phosphohydrolase | + | from API zym | |
| 120393 | ornithine decarboxylase | - | 4.1.1.17 | |
| 120393 | oxidase | + | ||
| 68382 | trypsin | - | 3.4.21.4 | from API zym |
| 120393 | urease | - | 3.5.1.5 | |
| 68382 | valine arylamidase | - | from API zym |
| @ref | ControlQ | GLY | ERY | DARA | LARA | RIB | DXYL | LXYL | ADO | MDX | GAL | GLU | FRU | MNE | SBE | RHA | DUL | INO | MAN | SOR | MDM | MDG | NAG | AMY | ARB | ESC | SAL | CEL | MAL | LAC | MEL | SAC | TRE | INU | MLZ | RAF | AMD | GLYG | XLT | GEN | TUR | LYX | TAG | DFUC | LFUC | DARL | LARL | GNT | 2KG | 5KG | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 120393 | not determinedn.d. | - | - | - | - | +/- | + | - | - | - | +/- | + | + | + | - | - | - | - | + | - | - | +/- | + | - | + | + | + | +/- | + | +/- | +/- | - | + | - | +/- | - | + | + | - | - | +/- | - | - | - | - | - | - | +/- | - | - |
| @ref | Description | Assembly level | INSDC accession | BV-BRC accession | IMG accession | NCBI tax ID | Score | |
|---|---|---|---|---|---|---|---|---|
| 66792 | ASM516006v1 assembly for Geobacillus kaustophilus NBRC 102445 | complete | 1220595 | 95.88 | ||||
| 67770 | ASM73995v1 assembly for Geobacillus kaustophilus NBRC 102445 | contig | 1220595 | 55.03 |
| @ref | Description | Accession | Length | Database | NCBI tax ID | |
|---|---|---|---|---|---|---|
| 20218 | Geobacillus kaustophilus strain BCRC 11223 16S ribosomal RNA gene, partial sequence | EU484357 | 1402 | 1462 | ||
| 20218 | Geobacillus kaustophilus isolate Gk9 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | EU723627 | 845 | 1462 | ||
| 20218 | Geobacillus kaustophilus isolate Gk11 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | EU723628 | 999 | 1462 | ||
| 20218 | Geobacillus kaustophilus isolate Gk12 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | EU723629 | 666 | 1462 | ||
| 20218 | Geobacillus kaustophilus isolate Gk24 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence | EU723630 | 865 | 1462 | ||
| 20218 | Geobacillus kaustophilus gene for 16S rRNA, partial sequence | AB006927 | 281 | 1462 | ||
| 20218 | Geobacillus kaustophilus gene for 16S rRNA, partial sequence, strain: NBRC 102445 | AB681787 | 1483 | 1462 | ||
| 20218 | B.kaustophilus 16S ribosomal RNA | X60618 | 1432 | 1462 |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125439 | oxygen_tolerance | BacteriaNetⓘ | facultative anaerobe | 63.50 | no |
| 125439 | gram_stain | BacteriaNetⓘ | variable | 77.90 | no |
| 125439 | motility | BacteriaNetⓘ | yes | 79.90 | no |
| 125439 | spore_formation | BacteriaNetⓘ | yes | 84.90 | no |
| @ref | Trait | Model | Prediction | Confidence in % | In training data |
|---|---|---|---|---|---|
| 125438 | gram-positive | gram-positiveⓘ | yes | 76.20 | yes |
| 125438 | anaerobic | anaerobicⓘ | no | 94.10 | no |
| 125438 | aerobic | aerobicⓘ | yes | 79.49 | yes |
| 125438 | spore-forming | spore-formingⓘ | yes | 92.47 | yes |
| 125438 | thermophilic | thermophileⓘ | yes | 65.85 | no |
| 125438 | flagellated | motile2+ⓘ | yes | 80.01 | no |
| Topic | Title | Authors | Journal | DOI | Year | |
|---|---|---|---|---|---|---|
| Similarities of Geobacillus bacteria based on their profiles of antimicrobial susceptibility in milk samples. | Nagel OG, Gasparotti ML, Machado SI, Althaus RL. | Rev Argent Microbiol | 10.1016/j.ram.2023.07.003 | 2024 | ||
| Enzymology | Nitrile as Activating Group in the Asymmetric Bioreduction of beta-Cyanoacrylic Acids Catalyzed by Ene-Reductases. | Winkler CK, Clay D, Turrini NG, Lechner H, Kroutil W, Davies S, Debarge S, O'Neill P, Steflik J, Karmilowicz M, Wong JW, Faber K. | Adv Synth Catal | 10.1002/adsc.201301055 | 2014 | |
| Metabolism | Overcoming co-product inhibition in the nicotinamide independent asymmetric bioreduction of activated C=C-bonds using flavin-dependent ene-reductases. | Winkler CK, Clay D, van Heerden E, Faber K. | Biotechnol Bioeng | 10.1002/bit.24981 | 2013 | |
| Metabolism | NAD(P)H-independent asymmetric C=C bond reduction catalyzed by ene reductases by using artificial co-substrates as the hydrogen donor. | Winkler CK, Clay D, Entner M, Plank M, Faber K. | Chemistry | 10.1002/chem.201303897 | 2014 | |
| Genetics | Development and implementation of rapid metabolic engineering tools for chemical and fuel production in Geobacillus thermoglucosidasius NCIMB 11955. | Sheng L, Kovacs K, Winzer K, Zhang Y, Minton NP. | Biotechnol Biofuels | 10.1186/s13068-016-0692-x | 2017 | |
| Metabolism | Identification and characterisation of capidermicin, a novel bacteriocin produced by Staphylococcus capitis. | Lynch D, O'Connor PM, Cotter PD, Hill C, Field D, Begley M. | PLoS One | 10.1371/journal.pone.0223541 | 2019 | |
| Metabolism | Asymmetric bioreduction of activated alkenes to industrially relevant optically active compounds. | Winkler CK, Tasnadi G, Clay D, Hall M, Faber K. | J Biotechnol | 10.1016/j.jbiotec.2012.03.023 | 2012 | |
| Enzymology | Functional Characterization, Genome Assembly, and Annotation of Geobacillus sp. G4 Isolated from a Geothermal Field in Tacna, Peru. | Ticona ARP, Santos KCR, Ramirez-Arua HE, Castellanos R, Silva JP, Hamann PRV, Noronha EF, Lopes FAC. | Microorganisms | 10.3390/microorganisms13061374 | 2025 | |
| Correlating the succession of microbial communities from Nigerian soils to petroleum biodegradation. | Iturbe-Espinoza P, Bonte M, Weedon JT, Braster M, Brandt BW, van Spanning RJ. | World J Microbiol Biotechnol | 10.1007/s11274-023-03656-7 | 2023 | ||
| Enzymology | Counterselection system for Geobacillus kaustophilus HTA426 through disruption of pyrF and pyrR. | Suzuki H, Murakami A, Yoshida K. | Appl Environ Microbiol | 10.1128/aem.01669-12 | 2012 | |
| Analysis of r-protein and RNA conformation of 30S subunit intermediates in bacteria. | Napper N, Culver GM. | RNA | 10.1261/rna.048918.114 | 2015 | ||
| Engineering of Geobacillus kaustophilus lipase for enhanced catalytic efficiency and methanol tolerance in biodiesel production from sunflower oil. | Tulek A, Poyraz Y, Sukur G, Pacal N, Ozdemir FI, Yildirim D, Essiz S. | Int J Biol Macromol | 10.1016/j.ijbiomac.2025.148467 | 2025 | ||
| Old yellow enzyme-catalyzed desymmetrizing desaturation. | Wang H, Ye Y. | Methods Enzymol | 10.1016/bs.mie.2025.08.018 | 2025 | ||
| Enzymology | Immobilization of L-asparaginase on genipin and divinyl sulfone cross-linked multi-walled carbon nanotubes and silica supports for acrylamide mitigation. | Tulek A, Sukur G, Ozdemir FI, Varan NE, Yildirim D. | Food Chem | 10.1016/j.foodchem.2025.145268 | 2025 | |
| Synergistic mechanisms of compound thermophilic bacteria on waste sludge hydrolysis and reduction: Extracellular polymers degradation, enzyme activity and microbial community changes. | Sui M, Miao K, Tang L, Zhao J, Jin C, Zhao Y, Guo L. | Sci Total Environ | 10.1016/j.scitotenv.2025.180311 | 2025 | ||
| Phylogeny | Integrated Proteomic and Molecular Identification of Thermophilic Geobacillus Strains from Algerian Desert Sands and Their Enzymatic Potential. | Hammadi AI, Merzoug M, Aireche M, Zater ZY, Bendida K, Brakna CN, Choubane S, Todorov SD, Saidi D. | Life (Basel) | 10.3390/life15081327 | 2025 | |
| Enhanced Thermostability of an Improved l-Arabinose Isomerase Mutant for d-Tagatose Synthesis by Molecular Dynamics Simulations-Guided Rational Redesign of Flexible Regions. | Ravikumar Y, Arsalan A, Tang X, Zhang G, Qi X. | J Agric Food Chem | 10.1021/acs.jafc.5c03396 | 2025 | ||
| Enzymology | Expression and characterization of a thermostable monoacylglycerol lipase from thermophilic Geobacillus kaustophilus. | Doukyu N, Ito H, Sugimoto K. | Prep Biochem Biotechnol | 10.1080/10826068.2024.2361147 | 2025 | |
| Phylogeny | Integrated Proteomic and Molecular Identification of Thermophilic Geobacillus Strains from Algerian Desert Sands and Their Enzymatic Potential | Hammadi A, Merzoug M, Aireche M, Zater Z, Bendida K, Brakna C, Choubane S, Todorov S, Saidi D. | Life (Basel) | 2025 | ||
| Raw starch degrading alkaline alpha-amylase from Geobacillus kaustophilus TSCCA02: Production, characterization, and its potential for application as a detergent additive. | Phonlamai A, Kingkaew T, Prajanket P, Sakdapetsiri C, Krajangsang S, Kitpreechavanich V, Lomthong T. | J Basic Microbiol | 10.1002/jobm.202300653 | 2024 | ||
| Enzymology | Two different alanine dehydrogenases from Geobacillus kaustophilus: Their biochemical characteristics and differential expression in vegetative cells and spores. | Maeno M, Ohmori T, Nukada D, Sakuraba H, Satomura T, Ohshima T. | Biochim Biophys Acta Proteins Proteom | 10.1016/j.bbapap.2023.140904 | 2023 | |
| Functional annotation of haloacid dehalogenase superfamily structural genomics proteins. | Pathira Kankanamge LS, Ruffner LA, Touch MM, Pina M, Beuning PJ, Ondrechen MJ. | Biochem J | 10.1042/bcj20230057 | 2023 | ||
| Investigation of molecular details of a bacterial cationic amino acid transporter (GkApcT) during arginine transportation using molecular dynamics simulation and umbrella sampling techniques. | Afshinpour M, Parsi P, Mahdiuni H. | J Mol Model | 10.1007/s00894-023-05670-w | 2023 | ||
| Enzymology | Exploring the Kinetics and Thermodynamics of a Novel Histidine Ammonia-Lyase from Geobacillus kaustophilus. | Salas-Garrucho FM, Carrillo-Moreno A, Contreras LM, Rodriguez-Vico F, Clemente-Jimenez JM, Las Heras-Vazquez FJ. | Int J Mol Sci | 10.3390/ijms251810163 | 2024 | |
| Enzymology | The bacterial tRNA-modifying enzyme tRNAIle lysidine synthetase is genetically conserved but catalytically variable. | Muraski MJ, Guinto FC, Nilsson EM, Dabdoub JB, Robinson SC, Jiang Y, Shen Z, Alexander RW. | J Biol Chem | 10.1016/j.jbc.2025.110688 | 2025 | |
| Genetics | Simple sequence repeat insertion induced stability and potential 'gain of function' in the proteins of extremophilic bacteria. | Mahfooz S, Shankar G, Narayan J, Singh P, Akhter Y. | Extremophiles | 10.1007/s00792-022-01265-0 | 2022 | |
| Functional analysis of a gene cluster for putative bacteriocin deduced from the genome sequence of Aeribacillus pallidus PI8. | Kita K, Yoshida S, Ishikawa S, Yoshida KI. | J Gen Appl Microbiol | 10.2323/jgam.2021.11.003 | 2022 | ||
| Metabolism | Structural and functional characterization of a putative carbonic anhydrase from Geobacillus kaustophilus reveals its cambialistic function. | Sridharan U, Ragunathan P, Kuramitsu S, Yokoyama S, Kumarevel T, Ponnuraj K. | Biochem Biophys Res Commun | 10.1016/j.bbrc.2021.02.036 | 2021 | |
| Metabolism | Identification of a repressor for the two iol operons required for inositol catabolism in Geobacillus kaustophilus. | Yoshida KI, Shirae Y, Nishimura R, Fukui K, Ishikawa S. | Microbiology (Reading) | 10.1099/mic.0.001008 | 2021 | |
| Genetics | The microbiome-restorative potential of ibezapolstat for the treatment of Clostridioides difficile infection is predicted through variant PolC-type DNA polymerase III in Lachnospiraceae and Oscillospiraceae. | McPherson JK, Hurdle JG, Baker ML, Hussain T, Kumar A, Garey KW. | Antimicrob Agents Chemother | 10.1128/aac.01679-24 | 2025 | |
| Transcriptome | Transcriptome and growth efficiency comparisons of recombinant thermophiles that produce thermolabile and thermostable proteins: implications for burden-based selection of thermostable proteins. | Suzuki H, Okumura Y, Mikawa Y, Takata M, Yoshimura S, Ohshiro T. | Extremophiles | 10.1007/s00792-021-01237-w | 2021 | |
| Metabolism | Novel serine/threonine-O-glycosylation with N-acetylneuraminic acid and 3-deoxy-D-manno-octulosonic acid by bacterial flagellin glycosyltransferases. | Khairnar A, Sunsunwal S, Babu P, Ramya TNC. | Glycobiology | 10.1093/glycob/cwaa084 | 2021 | |
| Genetics | Machine learning-guided discovery of thermophilic carbonic anhydrases from environmental metagenomes. | Pairoh S, Mhuantong W, Boonyapakron K, Yuvaniyama J, Kanokratana P, Bunterngsook B, Lekakarn H, Arunrattanamook N, Laothanachareon T, Champreda V. | Sci Rep | 10.1038/s41598-025-24713-1 | 2025 | |
| Asymmetric C-Alkylation of Nitroalkanes via Enzymatic Photoredox Catalysis. | Fu H, Qiao T, Carceller JM, MacMillan SN, Hyster TK. | J Am Chem Soc | 10.1021/jacs.2c12197 | 2023 | ||
| Identification, characterization and hydrolase producing performance of thermophilic bacteria: geothermal hot springs in the Eastern and Southeastern Anatolia Regions of Turkey. | Ulucay O, Gormez A, Ozic C. | Antonie Van Leeuwenhoek | 10.1007/s10482-021-01678-5 | 2022 | ||
| A New Tool for the Flexible Genetic Manipulation of Geobacillus kaustophilus. | Amatsu R, Mori K, Ishikawa S, Meijer WJJ, Yoshida KI. | Bio Protoc | 10.21769/bioprotoc.4502 | 2022 | ||
| A novel method for transforming Geobacillus kaustophilus with a chromosomal segment of Bacillus subtilis transferred via pLS20-dependent conjugation. | Mori K, Fukui K, Amatsu R, Ishikawa S, Verrone V, Wipat A, Meijer WJJ, Yoshida KI. | Microb Cell Fact | 10.1186/s12934-022-01759-8 | 2022 | ||
| Thermostable Bacterial Esterases From Lipase Family 1.5 Degrade Compostable Polyesters PBAT and PBSA. | Ahmed FH, Esquirol L, French NG, Rocha RA, Cass P, Scott C. | Microbiologyopen | 10.1002/mbo3.70144 | 2025 | ||
| Metabolism | Directed Computational Evolution of Quorum-Quenching Lactonases from the Amidohydrolase Superfamily. | Go MK, Zhao LN, Xue B, Supekar S, Robinson RC, Fan H, Yew WS. | Structure | 10.1016/j.str.2020.03.011 | 2020 | |
| Metabolism | Permeabilized Escherichia coli Whole Cells Containing Co-Expressed Two Thermophilic Enzymes Facilitate the Synthesis of scyllo-Inositol from myo-Inositol. | Li Y, Liu S, You C. | Biotechnol J | 10.1002/biot.201900191 | 2020 | |
| Metabolism | Directed Evolution of Quorum-Quenching Enzymes: A Method for the Construction of a Directed Evolution Platform and Characterization of a Quorum-Quenching Lactonase from Geobacillus kaustophilus. | Go MK, Chow JY, Yew WS. | Methods Mol Biol | 10.1007/978-1-4939-7309-5_24 | 2018 | |
| Improving Catalytic Efficiency of L-Arabinose Isomerase from Lactobacillus plantarum CY6 towards D-Galactose by Molecular Modification. | Lu C, Chen Z, Ravikumar Y, Zhang G, Tang X, Zhang Y, Zhao M, Sun W, Qi X. | Foods | 10.3390/foods13111727 | 2024 | ||
| Enzymology | Structure of Urocanate Hydratase from the protozoan Trypanosoma cruzi. | Boreiko S, Silva M, de F P Melo R, Silber AM, Iulek J. | Int J Biol Macromol | 10.1016/j.ijbiomac.2019.12.101 | 2020 | |
| Frequent Transposition of Multiple Insertion Sequences in Geobacillus kaustophilus HTA426. | Suzuki H, Taketani T, Tanabiki M, Ohara M, Kobayashi J, Ohshiro T. | Front Microbiol | 10.3389/fmicb.2021.650461 | 2021 | ||
| Production Optimization and Biochemical Characterization of Cellulase from Geobacillus sp. KP43 Isolated from Hot Spring Water of Nepal. | Khadka S, Khadka D, Poudel RC, Bhandari M, Baidya P, Sijapati J, Maharjan J. | Biomed Res Int | 10.1155/2022/6840409 | 2022 | ||
| Fate of oxygen species from O2 activation at dimetal cofactors in an oxidase enzyme revealed by 57Fe nuclear resonance X-ray scattering and quantum chemistry. | Mebs S, Srinivas V, Kositzki R, Griese JJ, Hogbom M, Haumann M. | Biochim Biophys Acta Bioenerg | 10.1016/j.bbabio.2019.148060 | 2019 | ||
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| Metabolism | Helicobacter pylori FlhB function: the FlhB C-terminal homologue HP1575 acts as a "spare part" to permit flagellar export when the HP0770 FlhBCC domain is deleted. | Wand ME, Sockett RE, Evans KJ, Doherty N, Sharp PM, Hardie KR, Winzer K. | J Bacteriol | 10.1128/jb.00263-06 | 2006 | |
| Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family. | Odokonyero D, Sakai A, Patskovsky Y, Malashkevich VN, Fedorov AA, Bonanno JB, Fedorov EV, Toro R, Agarwal R, Wang C, Ozerova ND, Yew WS, Sauder JM, Swaminathan S, Burley SK, Almo SC, Glasner ME. | Proc Natl Acad Sci U S A | 10.1073/pnas.1318703111 | 2014 | ||
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| Metabolism | Integrated multi-omics analyses reveal the biochemical mechanisms and phylogenetic relevance of anaerobic androgen biodegradation in the environment. | Yang FC, Chen YL, Tang SL, Yu CP, Wang PH, Ismail W, Wang CH, Ding JY, Yang CY, Yang CY, Chiang YR. | ISME J | 10.1038/ismej.2015.255 | 2016 | |
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| Metabolism | Kinase activity of overexpressed HipA is required for growth arrest and multidrug tolerance in Escherichia coli. | Correia FF, D'Onofrio A, Rejtar T, Li L, Karger BL, Makarova K, Koonin EV, Lewis K. | J Bacteriol | 10.1128/jb.01237-06 | 2006 | |
| Metabolism | ComEA is essential for the transfer of external DNA into the periplasm in naturally transformable Vibrio cholerae cells. | Seitz P, Pezeshgi Modarres H, Borgeaud S, Bulushev RD, Steinbock LJ, Radenovic A, Dal Peraro M, Blokesch M. | PLoS Genet | 10.1371/journal.pgen.1004066 | 2014 | |
| Metabolism | SR1--a small RNA with two remarkably conserved functions. | Gimpel M, Preis H, Barth E, Gramzow L, Brantl S. | Nucleic Acids Res | 10.1093/nar/gks895 | 2012 | |
| Genetics | Bacterial lifestyle in a deep-sea hydrothermal vent chimney revealed by the genome sequence of the thermophilic bacterium Deferribacter desulfuricans SSM1. | Takaki Y, Shimamura S, Nakagawa S, Fukuhara Y, Horikawa H, Ankai A, Harada T, Hosoyama A, Oguchi A, Fukui S, Fujita N, Takami H, Takai K. | DNA Res | 10.1093/dnares/dsq005 | 2010 | |
| Proteome | Role of key salt bridges in thermostability of G. thermodenitrificans EstGtA2: distinctive patterns within the new bacterial lipolytic enzyme subfamily XIII.2 [corrected]. | Charbonneau DM, Beauregard M. | PLoS One | 10.1371/journal.pone.0076675 | 2013 | |
| Phylogeny | Genome sequences of the biotechnologically important Bacillus megaterium strains QM B1551 and DSM319. | Eppinger M, Bunk B, Johns MA, Edirisinghe JN, Kutumbaka KK, Koenig SS, Creasy HH, Rosovitz MJ, Riley DR, Daugherty S, Martin M, Elbourne LD, Paulsen I, Biedendieck R, Braun C, Grayburn S, Dhingra S, Lukyanchuk V, Ball B, Ul-Qamar R, Seibel J, Bremer E, Jahn D, Ravel J, Vary PS. | J Bacteriol | 10.1128/jb.00449-11 | 2011 | |
| Genetics | Multiple genome alignment for identifying the core structure among moderately related microbial genomes. | Uchiyama I. | BMC Genomics | 10.1186/1471-2164-9-515 | 2008 | |
| Metabolism | Confirmation of a second natural preQ1 aptamer class in Streptococcaceae bacteria. | Meyer MM, Roth A, Chervin SM, Garcia GA, Breaker RR. | RNA | 10.1261/rna.937308 | 2008 | |
| CGAT: a comparative genome analysis tool for visualizing alignments in the analysis of complex evolutionary changes between closely related genomes. | Uchiyama I, Higuchi T, Kobayashi I. | BMC Bioinformatics | 10.1186/1471-2105-7-472 | 2006 | ||
| Metabolism | Fluorescent sensors for activity and regulation of the nitrate transceptor CHL1/NRT1.1 and oligopeptide transporters. | Ho CH, Frommer WB. | Elife | 10.7554/elife.01917 | 2014 | |
| Metabolism | Determinants of eukaryal cell killing by the bacterial ribotoxin PrrC. | Meineke B, Schwer B, Schaffrath R, Shuman S. | Nucleic Acids Res | 10.1093/nar/gkq831 | 2011 | |
| Genetics | Encapsulated in silica: genome, proteome and physiology of the thermophilic bacterium Anoxybacillus flavithermus WK1. | Saw JH, Mountain BW, Feng L, Omelchenko MV, Hou S, Saito JA, Stott MB, Li D, Zhao G, Wu J, Galperin MY, Koonin EV, Makarova KS, Wolf YI, Rigden DJ, Dunfield PF, Wang L, Alam M. | Genome Biol | 10.1186/gb-2008-9-11-r161 | 2008 | |
| Metabolism | TasA-tasB, a new putative toxin-antitoxin (TA) system from Bacillus thuringiensis pGI1 plasmid is a widely distributed composite mazE-doc TA system. | Fico S, Mahillon J. | BMC Genomics | 10.1186/1471-2164-7-259 | 2006 | |
| Metabolism | Thermostable group II intron reverse transcriptase fusion proteins and their use in cDNA synthesis and next-generation RNA sequencing. | Mohr S, Ghanem E, Smith W, Sheeter D, Qin Y, King O, Polioudakis D, Iyer VR, Hunicke-Smith S, Swamy S, Kuersten S, Lambowitz AM. | RNA | 10.1261/rna.039743.113 | 2013 | |
| Metabolism | Biochemical and molecular characterization of the biosynthesis of glutamine and glutamate, two major compatible solutes in the moderately halophilic bacterium Halobacillus halophilus. | Saum SH, Sydow JF, Palm P, Pfeiffer F, Oesterhelt D, Muller V. | J Bacteriol | 10.1128/jb.00781-06 | 2006 | |
| Enzymology | Functional and structural characterization of four glutaminases from Escherichia coli and Bacillus subtilis. | Brown G, Singer A, Proudfoot M, Skarina T, Kim Y, Chang C, Dementieva I, Kuznetsova E, Gonzalez CF, Joachimiak A, Savchenko A, Yakunin AF. | Biochemistry | 10.1021/bi800097h | 2008 | |
| Phylogeny | Stratification of co-evolving genomic groups using ranked phylogenetic profiles. | Freilich S, Goldovsky L, Gottlieb A, Blanc E, Tsoka S, Ouzounis CA. | BMC Bioinformatics | 10.1186/1471-2105-10-355 | 2009 | |
| Metabolism | Cross-regulation of the Bacillus subtilis glnRA and tnrA genes provides evidence for DNA binding site discrimination by GlnR and TnrA. | Zalieckas JM, Wray LV, Fisher SH. | J Bacteriol | 10.1128/jb.188.7.2578-2585.2006 | 2006 | |
| A widespread peroxiredoxin-like domain present in tumor suppression- and progression-implicated proteins. | Pawlowski K, Muszewska A, Lenart A, Szczepinska T, Godzik A, Grynberg M. | BMC Genomics | 10.1186/1471-2164-11-590 | 2010 | ||
| Transcriptomic and phenotypic analyses suggest a network between the transcriptional regulators HrcA and sigmaB in Listeria monocytogenes. | Hu Y, Oliver HF, Raengpradub S, Palmer ME, Orsi RH, Wiedmann M, Boor KJ. | Appl Environ Microbiol | 10.1128/aem.01281-07 | 2007 | ||
| Enzymology | Comprehensive analysis of DNA polymerase III alpha subunits and their homologs in bacterial genomes. | Timinskas K, Balvociute M, Timinskas A, Venclovas C. | Nucleic Acids Res | 10.1093/nar/gkt900 | 2014 | |
| Metabolism | Molecular basis of TRAP-5'SL RNA interaction in the Bacillus subtilis trp operon transcription attenuation mechanism. | McGraw AP, Mokdad A, Major F, Bevilacqua PC, Babitzke P. | RNA | 10.1261/rna.1314409 | 2009 | |
| The Bet v 1 fold: an ancient, versatile scaffold for binding of large, hydrophobic ligands. | Radauer C, Lackner P, Breiteneder H. | BMC Evol Biol | 10.1186/1471-2148-8-286 | 2008 | ||
| Phylogeny | A phylogenomic profile of globins. | Vinogradov SN, Hoogewijs D, Bailly X, Arredondo-Peter R, Gough J, Dewilde S, Moens L, Vanfleteren JR. | BMC Evol Biol | 10.1186/1471-2148-6-31 | 2006 | |
| Metabolism | AMPylation targets the rate-limiting step of BiP's ATPase cycle for its functional inactivation. | Preissler S, Rohland L, Yan Y, Chen R, Read RJ, Ron D. | Elife | 10.7554/elife.29428 | 2017 | |
| Metabolism | Serine proteases from two cell types target different components of a complex that governs regulated intramembrane proteolysis of pro-sigmaK during Bacillus subtilis development. | Zhou R, Kroos L. | Mol Microbiol | 10.1111/j.1365-2958.2005.04870.x | 2005 | |
| Bioinformatic characterization of the trimeric intracellular cation-specific channel protein family. | Silverio AL, Saier MH. | J Membr Biol | 10.1007/s00232-011-9364-8 | 2011 | ||
| Genetics | Relationship between operon preference and functional properties of persistent genes in bacterial genomes. | Bratlie MS, Johansen J, Drablos F. | BMC Genomics | 10.1186/1471-2164-11-71 | 2010 | |
| Metabolism | Physiological modulation of BiP activity by trans-protomer engagement of the interdomain linker. | Preissler S, Chambers JE, Crespillo-Casado A, Avezov E, Miranda E, Perez J, Hendershot LM, Harding HP, Ron D. | Elife | 10.7554/elife.08961 | 2015 | |
| Metabolism | Dancing through Life: Molecular Dynamics Simulations and Network-Centric Modeling of Allosteric Mechanisms in Hsp70 and Hsp110 Chaperone Proteins. | Stetz G, Verkhivker GM. | PLoS One | 10.1371/journal.pone.0143752 | 2015 | |
| Post-transcriptional control by bacteriophage T4: mRNA decay and inhibition of translation initiation. | Uzan M, Miller ES. | Virol J | 10.1186/1743-422x-7-360 | 2010 | ||
| Genetics | Survey of group I and group II introns in 29 sequenced genomes of the Bacillus cereus group: insights into their spread and evolution. | Tourasse NJ, Kolsto AB. | Nucleic Acids Res | 10.1093/nar/gkn372 | 2008 | |
| Effects of small heat shock proteins from thermotolerant bacteria on the stress resistance of Escherichia coli to temperature, pH, and hyperosmolarity. | Sato Y, Okano K, Honda K. | Extremophiles | 10.1007/s00792-023-01326-y | 2024 | ||
| Genetics | Unveiling the probiotic potential of the genus Geobacillus through comparative genomics and in silico analysis. | Najar IN, Sharma P, Das R, Mondal K, Singh AK, Radha A, Sharma V, Sharma S, Thakur N, Gandhi SG, Kumar V. | Sci Rep | 10.1038/s41598-025-98392-3 | 2025 | |
| A tRNA modification with aminovaleramide facilitates AUA decoding in protein synthesis. | Miyauchi K, Kimura S, Akiyama N, Inoue K, Ishiguro K, Vu TS, Srisuknimit V, Koyama K, Hayashi G, Soma A, Nagao A, Shirouzu M, Okamoto A, Waldor MK, Suzuki T. | Nat Chem Biol | 10.1038/s41589-024-01726-x | 2025 | ||
| Expression and Purification of BsaXI Restriction Endonuclease and Engineering New Specificity From BsaXI Specificity Subunit. | Gidwani S, Heiter D, Xu SY. | Front Microbiol | 10.3389/fmicb.2022.888435 | 2022 | ||
| Genetics | Genome Annotation of Poly(lactic acid) Degrading Pseudomonas aeruginosa, Sphingobacterium sp. and Geobacillus sp. | Satti SM, Castro-Aguirre E, Shah AA, Marsh TL, Auras R. | Int J Mol Sci | 10.3390/ijms22147385 | 2021 | |
| Enzymology | Identification and Heterologous Production of a Lipase from Geobacillus kaustophilus DSM 7263(T) and Tailoring Its N-Terminal by a His-Tag Epitope. | Ozdemir FI, Tulek A, Erdogan D | Protein J | 10.1007/s10930-021-09987-4 | 2021 | |
| Genetics | Draft Genome Sequence of Geobacillus kaustophilus GBlys, a Lysogenic Strain with Bacteriophage OH2. | Doi K, Mori K, Martono H, Nagayoshi Y, Fujino Y, Tashiro K, Kuhara S, Ohshima T | Genome Announc | 10.1128/genomeA.00634-13 | 2013 | |
| Phylogeny | Isolation and characterization of arsenic resistant Geobacillus kaustophilus strain from geothermal soils. | Cuebas M, Sannino D, Bini E | J Basic Microbiol | 10.1002/jobm.201000314 | 2011 | |
| Enzymology | Biochemical characterization and detection of antitumor activity of l-asparaginase from thermophilic Geobacillus kaustophilus DSM 7263(T). | Ozdemir FI, Didem Orhan M, Atasavum ZT, Tulek A | Protein Expr Purif | 10.1016/j.pep.2022.106146 | 2022 | |
| Phylogeny | Geobacillus zalihae sp. nov., a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia. | Abd Rahman RN, Leow TC, Salleh AB, Basri M | BMC Microbiol | 10.1186/1471-2180-7-77 | 2007 | |
| Phylogeny | Geobacillus thermoleovorans subsp. stromboliensis subsp. nov., isolated from the geothermal volcanic environment. | Romano I, Poli A, Lama L, Gambacorta A, Nicolaus B | J Gen Appl Microbiol | 10.2323/jgam.51.183 | 2005 |
| #3082 | Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 7263 |
| #20215 | Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 ) |
| #20218 | Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 ) |
| #23044 | T. N. Nazina,T. P. Tourova,A. B. Poltaraus,E. V. Novikova,A. A. Grigoryan,A. E. Ivanova,A. M. Lysenko,V. V. Petrunyaka,G. A. Osipov,S. S. Belyaev,M. V. Ivanov: Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. th.. IJSEM 51: 433 - 446 2001 ( DOI 10.1099/00207713-51-2-433 , PubMed 11321089 ) |
| #38651 | ; Curators of the CIP; |
| #49445 | Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 28890 |
| #66792 | Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) . |
| #67770 | Japan Collection of Microorganism (JCM) ; Curators of the JCM; |
| #68371 | Automatically annotated from API 50CH acid . |
| #68382 | Automatically annotated from API zym . |
| #120393 | Collection of Institut Pasteur ; Curators of the CIP; CIP 106929 |
| #125438 | Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 ) |
| #125439 | Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 . |
| #126262 | A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 ) |
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