Name: Spiroplasma syrphidicola EA-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14368

Type strain:

Species: Spiroplasma syrphidicola

Strain Designation: EA-1

Culture col. no.: DSM 22631, ATCC 33826

Strain history: <- J. K. Davis, Dept. Agronomy, Purdue Univ., West Lafayette, USA <- J. G. Tully <- T. Clark, USDA; EA-1

NCBI tax ID(s): 1276229 (strain), 216945 (species)

SI-ID 40711

ATCC 33826

Section

Spiroplasma syrphidicola EA-1 is a microaerophile, mesophilic bacterium that was isolated from hemolymph of the syrphid fly Eristalis arbustorum.

mesophilic
microaerophile
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Mycoplasmatota*
	Class	*Mollicutes*
	Order	*Mycoplasmatales*
	Family	*Spiroplasmataceae*
	Genus	*Spiroplasma*
	Species	**Spiroplasma syrphidicola**
	Full Scientific Name (LPSN)	Spiroplasma syrphidicola Whitcomb et al. 1996

Information on culture and growth conditions Culture and growth conditions

[Ref.: #16439]

Culture medium

SP4-Z MEDIUM (DSMZ Medium 1076b)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #16439]			growth	30 °C

[Ref.: #16439]

Temperature range

mesophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #16439]

Oxygen tolerance

microaerophile

	Pathway	Enzyme coverage	Annotated reactions
	Pathway annotation	Computationally determined by
[Ref.: #66794]	folate polyglutamylation	100	1 of 1
[Ref.: #66794]	formaldehyde oxidation	100	3 of 3
[Ref.: #66794]	CDP-diacylglycerol biosynthesis	100	2 of 2
[Ref.: #66794]	coenzyme A metabolism	100	4 of 4
[Ref.: #66794]	palmitate biosynthesis	77.27	17 of 22
[Ref.: #66794]	ppGpp biosynthesis	75	3 of 4
[Ref.: #66794]	photosynthesis	71.43	10 of 14
[Ref.: #66794]	glycolysis	70.59	12 of 17
[Ref.: #66794]	acetoin degradation	66.67	2 of 3
[Ref.: #66794]	L-lactaldehyde degradation	66.67	2 of 3
[Ref.: #66794]	aspartate and asparagine metabolism	66.67	6 of 9
[Ref.: #66794]	UDP-GlcNAc biosynthesis	66.67	2 of 3
[Ref.: #66794]	NAD metabolism	55.56	10 of 18
[Ref.: #66794]	d-mannose degradation	55.56	5 of 9
[Ref.: #66794]	glycogen biosynthesis	50	2 of 4
[Ref.: #66794]	sulfopterin metabolism	50	2 of 4
[Ref.: #66794]	adipate degradation	50	1 of 2
[Ref.: #66794]	acetate fermentation	50	2 of 4
[Ref.: #66794]	ethanol fermentation	50	1 of 2
[Ref.: #66794]	ketogluconate metabolism	50	4 of 8
[Ref.: #66794]	ribulose monophosphate pathway	50	1 of 2
[Ref.: #66794]	purine metabolism	46.81	44 of 94
[Ref.: #66794]	pentose phosphate pathway	45.45	5 of 11
[Ref.: #66794]	serine metabolism	44.44	4 of 9
[Ref.: #66794]	degradation of sugar alcohols	43.75	7 of 16
[Ref.: #66794]	propanol degradation	42.86	3 of 7
[Ref.: #66794]	cardiolipin biosynthesis	42.86	3 of 7
[Ref.: #66794]	pyrimidine metabolism	42.22	19 of 45
[Ref.: #66794]	methylglyoxal degradation	40	2 of 5
[Ref.: #66794]	flavin biosynthesis	40	6 of 15
[Ref.: #66794]	lipoate biosynthesis	40	2 of 5
[Ref.: #66794]	metabolism of amino sugars and derivatives	40	2 of 5
[Ref.: #66794]	cellulose degradation	40	2 of 5
[Ref.: #66794]	phenylalanine metabolism	38.46	5 of 13
[Ref.: #66794]	vitamin B1 metabolism	38.46	5 of 13
[Ref.: #66794]	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	37.5	3 of 8
[Ref.: #66794]	d-xylose degradation	36.36	4 of 11
[Ref.: #66794]	metabolism of disaccharids	36.36	4 of 11
[Ref.: #66794]	oxidative phosphorylation	36.26	33 of 91
[Ref.: #66794]	tetrahydrofolate metabolism	35.71	5 of 14
[Ref.: #66794]	isoprenoid biosynthesis	34.62	9 of 26
[Ref.: #66794]	non-pathway related	34.21	13 of 38
[Ref.: #66794]	glycolate and glyoxylate degradation	33.33	2 of 6
[Ref.: #66794]	lipid A biosynthesis	33.33	3 of 9
[Ref.: #66794]	acetyl CoA biosynthesis	33.33	1 of 3
[Ref.: #66794]	octane oxidation	33.33	1 of 3
[Ref.: #66794]	cyanate degradation	33.33	1 of 3
[Ref.: #66794]	IAA biosynthesis	33.33	1 of 3
[Ref.: #66794]	selenocysteine biosynthesis	33.33	2 of 6
[Ref.: #66794]	lipid metabolism	32.26	10 of 31
[Ref.: #66794]	glutamate and glutamine metabolism	32.14	9 of 28
[Ref.: #66794]	urea cycle	30.77	4 of 13
[Ref.: #66794]	methionine metabolism	30.77	8 of 26
[Ref.: #66794]	myo-inositol biosynthesis	30	3 of 10
[Ref.: #66794]	Entner Doudoroff pathway	30	3 of 10
[Ref.: #66794]	reductive acetyl coenzyme A pathway	28.57	2 of 7
[Ref.: #66794]	glutathione metabolism	28.57	4 of 14
[Ref.: #66794]	degradation of pentoses	25	7 of 28
[Ref.: #66794]	butanoate fermentation	25	1 of 4
[Ref.: #66794]	cyclohexanol degradation	25	1 of 4
[Ref.: #66794]	C4 and CAM-carbon fixation	25	2 of 8
[Ref.: #66794]	biotin biosynthesis	25	1 of 4
[Ref.: #66794]	lactate fermentation	25	1 of 4
[Ref.: #66794]	CMP-KDO biosynthesis	25	1 of 4
[Ref.: #66794]	degradation of aromatic, nitrogen containing compounds	25	3 of 12
[Ref.: #66794]	leucine metabolism	23.08	3 of 13
[Ref.: #66794]	ascorbate metabolism	22.73	5 of 22
[Ref.: #66794]	cysteine metabolism	22.22	4 of 18
[Ref.: #66794]	valine metabolism	22.22	2 of 9
[Ref.: #66794]	CO2 fixation in Crenarchaeota	22.22	2 of 9
[Ref.: #66794]	tyrosine metabolism	21.43	3 of 14
[Ref.: #66794]	alanine metabolism	20.69	6 of 29
		Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #16439]	Sample type/isolated from	hemolymph of the syrphid fly Eristalis arbustorum
[Ref.: #16439]	Host species	Eristalis arbustorum
* marker position based on {}

Isolation sources categories

#Host	#Arthropoda	#Insecta
#Host Body Product	#Fluids	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence NR_025711 (>99% sequence identity) for Spiroplasma from Microbeatlas

Aquatic Samples	54
Soil Samples	6
Animal Samples	67
Plant Samples	3
Total Samples	130

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #16439]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #16439]	Spiroplasma syrphidicola strain EA-1 16S ribosomal RNA, partial sequence	NR_025711	1516	GenBank	216945 tax ID
[Ref.: #20218]	Spiroplasma syrphidicola 16S ribosomal RNA gene, partial sequence	AY189309	1516	ENA	1276229 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Spiroplasma syrphidicola EA-1	GCA_000400955	complete	GenBank	1276229 tax ID	*
[Ref.: #66792]	Spiroplasma syrphidicola EA-1	1276229.3	complete	PATRIC	1276229 tax ID	*
[Ref.: #66792]	Spiroplasma syrphidicola EA-1	2554235381	complete	JGI IMG/M	1276229 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Spiroplasma syrphidicola EA-1 EA-1 NCBI: GCA_000400955.1
[Ref.: #69480]	motile	no	92.82	no
[Ref.: #69480]	flagellated	no	98.173	no
[Ref.: #69480]	gram-positive	yes	71.234	no
[Ref.: #69480]	anaerobic	no	94.971	no
[Ref.: #69480]	aerobic	no	98.152	no
[Ref.: #69480]	halophile	yes	69.257	no
[Ref.: #69480]	spore-forming	no	98.464	no
[Ref.: #69480]	thermophile	no	93.311	yes
[Ref.: #69480]	glucose-util	yes	88.794	no
[Ref.: #69480]	glucose-ferment	yes	76.378	no

Availability in culture collections External links

[Ref.: #16439]

Culture collection no.

DSM 22631, ATCC 33826

[Ref.: #83511]

SI-ID 40711

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Spiroplasma syrphidicola sp. nov., from a syrphid fly (Diptera: Syrphidae).	Whitcomb RF, Gasparich GE, French FE, Tully JG, Rose DL, Carle P, Bove JM, Henegar RB, Konai M, Hackett KJ, Adams JR, Clark TB, Williamson DL	Int J Syst Bacteriol	10.1099/00207713-46-3-797	1996	*

References

#16439

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 22631

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#66794

Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#83511

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID40711.1 )

* These data were automatically processed and therefore are not curated

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Spiroplasma syrphidicola EA-1 EA-1

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