Clostridioides difficile (CCUG 20309)

Name: Clostridioides difficile (CCUG 20309)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 143578

Type strain

Culture col. no. CCUG 20309

NCBI tax ID(s) 1496

Special Collections CCUG

Links

Clostridioides difficile CCUG 20309 is an anaerobe bacterium that was isolated from Human clinical specimen.

anaerobe Bacteria

Name and taxonomic classification

SI-ID 52883

CCUG 20309

@ref 20215

Last LPSN update 2026-05-29 08:40:28

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Peptostreptococcales

Family Peptostreptococcaceae

Genus Clostridioides

Species Clostridioides difficile

Full scientific name Clostridioides difficile (Hall and O'Toole 1935) Lawson et al. 2016

Synonyms (3)

"Peptoclostridium difficile"
"Bacillus difficilis"
Clostridium difficile

BacDive ID	Other strains from **Clostridioides difficile** (81)	Type strain
2582	C. difficile 1780, McClung 1780, 90556-M6S, 10008, DSM ... (type strain)
2583	C. difficile AA1, DSM 12056
2584	C. difficile A90, DSM 12057
22963	C. difficile R20291, DSM 27147, NCTC 13366
23853	C. difficile 630, DSM 27543, ATCC BAA-1382, NCTC 13307
23854	C. difficile 1470, DSM 27544, ATCC 43598, NCTC 13569, ...
23855	C. difficile 630 Delta erm, DSM 28645
134676	C. difficile CD196, CIP 107932
134703	C. difficile CIP 109239
135237	C. difficile W 1194, CIP 104903, ATCC 43594, CCUG 37779
135760	C. difficile CIP 110322
137137	C. difficile CIP 109238
137139	C. difficile CIP 109240, ATCC 43255, CCUG 19126, VPI 10463
138126	C. difficile 2149, CIP 104905, ATCC 43600, CCUG 37784
138128	C. difficile 545, CIP 104904, ATCC 43596, CCUG 37766
141963	C. difficile CCUG 8884
141965	C. difficile CCUG 9004
141966	C. difficile CCUG 9018
143948	C. difficile CCUG 22019
143949	C. difficile CCUG 22020
143950	C. difficile CCUG 22023
145666	C. difficile CCUG 29183
148544	C. difficile CCUG 35620
149446	C. difficile CCUG 37767
149447	C. difficile CCUG 37768
149448	C. difficile CCUG 37769
149449	C. difficile CCUG 37770
149450	C. difficile CCUG 37771
149451	C. difficile CCUG 37772
149452	C. difficile CCUG 37773
149453	C. difficile CCUG 37774
149454	C. difficile CCUG 37775
149455	C. difficile CCUG 37776
149456	C. difficile CCUG 37777
149457	C. difficile CCUG 37778
149458	C. difficile CCUG 37780, ATCC 43593
149459	C. difficile CCUG 37781, ATCC 43597
149460	C. difficile CCUG 37783, ATCC 43599
149461	C. difficile CCUG 37785, ATCC 43601
149462	C. difficile CCUG 37787, ATCC 43603
149536	C. difficile CCUG 37922
151958	C. difficile CCUG 45344
151959	C. difficile CCUG 45345
152338	C. difficile CCUG 46468
152339	C. difficile CCUG 46469
152340	C. difficile CCUG 46470
154467	C. difficile CCUG 53741
154468	C. difficile CCUG 53742
154474	C. difficile CCUG 53749
154520	C. difficile CCUG 54206
156075	C. difficile CCUG 60275, LBM 08.01.040
156076	C. difficile CCUG 60276, LBM 08.01.058
156768	C. difficile CCUG 64977
156771	C. difficile CCUG 65028
157662	C. difficile 4167/2013 1, DSM 27638
157663	C. difficile 4167/2013 2, DSM 27639
157664	C. difficile 4167/2013 3, DSM 27640
157665	C. difficile SC037-01-01, 6551/2013, DSM 28196
157666	C. difficile SC052-01-01, DSM 29688
157667	C. difficile SC009-01-01, DSM 29745
157668	C. difficile SC052-02-01, DSM 29747
157669	C. difficile CD-16-113, D/6474/2015, DSM 102978
157670	C. difficile 3525/15, Goe-91, DSM 105001
158911	C. difficile 3022, CD3022, DSM 101085
165126	C. difficile JCM 5243
165127	C. difficile JCM 5244
165128	C. difficile JCM 5245
165129	C. difficile JCM 5246
165130	C. difficile JCM 5247
165131	C. difficile JCM 5248
165132	C. difficile JCM 5249
165133	C. difficile JCM 5250
165134	C. difficile JCM 5251
165135	C. difficile JCM 5252
165136	C. difficile JCM 5253
165137	C. difficile JCM 5254
165138	C. difficile JCM 5256
165139	C. difficile JCM 5257
165140	C. difficile JCM 5258
176413	C. difficile RT 023, DSM 102859
176414	C. difficile RT 127, DSM 102860

Morphology

Culture and growth conditions

Culture temp

@ref	Growth	Type	Temperature (°C)
47126	positive	growth	37

Physiology and metabolism

Oxygen tolerance

47126

Oxygen toleranceanaerobe

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Human	-
#Infection	#Patient	-
#Infection	#Medical environment	#Clinic
#Infection	#Patient	#Specimen

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
47126	Human clinical specimen	Homo sapiens	Birmingham,The General Hospital	United Kingdom	GBR	Europe

Interaction and safety

Sequence information

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	ADP-ribosylating binary toxin genes of Clostridium difficile strain CCUG 20309.	Chang SY, Song KP.	DNA Seq	10.3109/10425170109047564	2001
Metabolism	Development of TaqMan-based quantitative PCR for sensitive and selective detection of toxigenic Clostridium difficile in human stools.	Kubota H, Sakai T, Gawad A, Makino H, Akiyama T, Ishikawa E, Oishi K.	PLoS One	10.1371/journal.pone.0111684	2014
	Clonal spread of a Clostridium difficile strain with a complete set of toxin A, toxin B, and binary toxin genes among Polish patients with Clostridium difficile-associated diarrhea.	Pituch H, Kreft D, Obuch-Woszczatynski P, Wultanska D, Meisel-Mikolajczyk F, Luczak M, van Belkum A.	J Clin Microbiol	10.1128/jcm.43.1.472-475.2005	2005
Phylogeny	Increased sporulation rate of epidemic Clostridium difficile Type 027/NAP1.	Akerlund T, Persson I, Unemo M, Noren T, Svenungsson B, Wullt M, Burman LG.	J Clin Microbiol	10.1128/jcm.01964-07	2008
Metabolism	Toxin profiles and resistances to macrolides and newer fluoroquinolones as epidemicity determinants of clinical isolates of Clostridium difficile from Warsaw, Poland.	Pituch H, van Leeuwen W, Maquelin K, Wultanska D, Obuch-Woszczatynski P, Nurzynska G, Kato H, Reijans M, Meisel-Mikolajczyk F, Luczak M, van Belkum A.	J Clin Microbiol	10.1128/jcm.00306-07	2007
Pathogenicity	Recombinant antigens based on toxins A and B of Clostridium difficile that evoke a potent toxin-neutralising immune response.	Maynard-Smith M, Ahern H, McGlashan J, Nugent P, Ling R, Denton H, Coxon R, Landon J, Roberts A, Shone C.	Vaccine	10.1016/j.vaccine.2013.11.099	2014
Enzymology	Automated detection of toxigenic Clostridium difficile in clinical samples: isothermal tcdB amplification coupled to array-based detection.	Hicke B, Pasko C, Groves B, Ager E, Corpuz M, Frech G, Munns D, Smith W, Warcup A, Denys G, Ledeboer NA, Lindsey W, Owen C, Rea L, Jenison R.	J Clin Microbiol	10.1128/jcm.00621-12	2012
Phylogeny	International typing study of toxin A-negative, toxin B-positive Clostridium difficile variants.	Johnson S, Sambol SP, Brazier JS, Delmee M, Avesani V, Merrigan MM, Gerding DN.	J Clin Microbiol	10.1128/jcm.41.4.1543-1547.2003	2003
	Binary toxin production in Clostridium difficile is regulated by CdtR, a LytTR family response regulator.	Carter GP, Lyras D, Allen DL, Mackin KE, Howarth PM, O'Connor JR, Rood JI.	J Bacteriol	10.1128/jb.00731-07	2007
Metabolism	Toxins, butyric acid, and other short-chain fatty acids are coordinately expressed and down-regulated by cysteine in Clostridium difficile.	Karlsson S, Lindberg A, Norin E, Burman LG, Akerlund T.	Infect Immun	10.1128/iai.68.10.5881-5888.2000	2000
	Characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile.	Lyerly DM, Barroso LA, Wilkins TD, Depitre C, Corthier G.	Infect Immun	10.1128/iai.60.11.4633-4639.1992	1992
Pathogenicity	Nucleotide and peptide sequences of the open reading frame encoding a truncated toxin A gene of Clostridium difficile strain CCUG 20309.	Song KP, Bai XL, Chang SY	DNA Seq	10.3109/10425179909008423	1999
Pathogenicity	A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection.	Negm OH, Hamed M, Monaghan TM	J Vis Exp	10.3791/57399	2018
	High prevalence of subclass-specific binding and neutralizing antibodies against Clostridium difficile toxins in adult cystic fibrosis sera: possible mode of immunoprotection against symptomatic C. difficile infection.	Monaghan TM, Negm OH, MacKenzie B, Hamed MR, Shone CC, Humphreys DP, Acharya KR, Wilcox MH	Clin Exp Gastroenterol	10.2147/CEG.S133939	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#47126	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 20309
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Clostridioides difficile (CCUG 20309)

Name and taxonomic classification

Morphology

Culture and growth conditions

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Sequence information

Genome-based predictions

Literature

Literature

References

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