Cite this strain

Thiospirochaeta perfilievii (DSM 19205, VKM B-2514, P)

BacDive ID 14339

Type strain

Strain Designation P

Culture col. no. DSM 19205 VKM B-2514 P

NCBI tax ID(s) 252967

Special Collections DSMZ Literature strains

History DSMZ <- G. A. Dubinina, Winogradsky Inst. Microbiol., RAS, Moscow, Russia; strain P <- G. A. Dubinina {1991}

Links

Thiospirochaeta perfilievii P is an anaerobe, Gram-negative, motile bacterium that was isolated from "Thiodendron" bacterial sulfur mat .

Gram-negative motile spiral-shaped anaerobe genome sequence 16S sequence Bacteria

Name and taxonomic classification

SI-ID 398000

VKM B-2514,DSM 19205

@ref 20215

Last LPSN update 2026-05-29 08:28:15

Domain Bacteria

Phylum Spirochaetota

Class Spirochaetia

Order Spirochaetales

Family Spirochaetaceae

Genus Thiospirochaeta

Species Thiospirochaeta perfilievii

Full scientific name Thiospirochaeta perfilievii (Dubinina et al. 2011) Dubinina et al. 2020

Synonyms (1)

Spirochaeta perfilievii

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
29667	negative	105 µm	0.3 µm	spiral-shaped		gliding

Colony morphology

7967

Incubation period1-2 days

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
7967	SEDIMINISPIROCHAETA MEDIUM (DSMZ Medium 1008)		Medium recipe at MediaDive	Name: SEDIMINISPIROCHAETA MEDIUM (DSMZ Medium 1008; with strain-specific modifications) Composition: D-Glucose 5.0 g/l Trypticase peptone 2.0 g/l Na-thioglycolate 1.0 g/l Yeast extract 1.0 g/l Sodium resazurin 0.0005 g/l Charcoal-filtered, natural seawater Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)
7967	positive	growth	28
29667	positive	growth	04-32
29667	positive	optimum	29

Culture pH

@ref	Ability	Type	PH	PH range
29667	positive	optimum	7.25
29667	positive	growth	6.5-8.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
7967	anaerobe
29667	anaerobe
125439	facultative anaerobe	90.389

Spore formation

@ref	Spore formation	Confidence
125439		90.954

Halophily

@ref	Salt	Growth	Tested relation	Concentration
29667	NaCl	positive	growth	0.58-5.8 %
29667	NaCl	positive	optimum	2.03 %

Observation

29667

Observationaggregates in clumps

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
29667	22653 ChEBI	asparagine	+	carbon source
29667	17057 ChEBI	cellobiose	+	carbon source
68380	16024 ChEBI	D-mannose	-	fermentation	from API rID32A
29667	17234 ChEBI	glucose	+	carbon source
29667	28087 ChEBI	glycogen	+	carbon source
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
29667	17306 ChEBI	maltose	+	carbon source
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
68380	16634 ChEBI	raffinose	-	fermentation	from API rID32A
29667	17992 ChEBI	sucrose	+	carbon source
29667	27082 ChEBI	trehalose	+	carbon source
68380	27897 ChEBI	tryptophan	-	energy source	from API rID32A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68380	35581 ChEBI	indole		from API rID32A

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	-	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68380	alanine arylamidase	+	3.4.11.2	from API rID32A
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68380	alpha-galactosidase	-	3.2.1.22	from API rID32A
68380	alpha-glucosidase	+	3.2.1.20	from API rID32A
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68380	beta-glucosidase	+	3.2.1.21	from API rID32A
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	+		from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
68380	N-acetyl-beta-glucosaminidase	+	3.2.1.52	from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68380	tryptophan deaminase	-	4.1.99.1	from API rID32A
68380	urease	-	3.5.1.5	from API rID32A

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
7967	-	-	-	+/-	-	+	+	-	-	+	-	-	-	-	-	-	-	+	-	-	+/-	+/-	-	+/-	+	+/-	-	-	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Spring
#Environmental	#Microbial community	#Microbial mat
#Condition	#Sulfuric	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
7967	"Thiodendron" bacterial sulfur mat (mineral sulfide spring)	Novgorod Region, Staraya Russa resort	Russia	RUS	Europe

Taxonmaps

69479

Global distribution of 16S sequence AY337318 (>99% sequence identity) for Thiospirochaeta perfilievii subclade from Microbeatlas

Aquatic Samples	4385
Soil Samples	99
Animal Samples	48
Plant Samples	60
Total Samples	4592

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
7967	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM832994v1 assembly for Thiospirochaeta perfilievii P	complete	GCA_008329945	252967.3	2988895331	252967	95.11

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
7967	Spirochaeta perfilievii strain P 16S ribosomal RNA gene, partial sequence	AY337318	1428		252967

GC content

@ref	GC-content (mol%)	Method
7967	41.7	thermal denaturation, midpoint method (Tm)
29667	41.7

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: ASM832994v1 assembly for Thiospirochaeta perfilievii P
BV-BRC: 252967.3

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	oxygen_tolerance	BacteriaNetⓘ	facultative anaerobe	90.39	no
125439	motility	BacteriaNetⓘ	no	70.81	no
125439	gram_stain	BacteriaNetⓘ	negative	79.26	no
125439	spore_formation	BacteriaNetⓘ	no	90.95	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Thiospirochaeta perfilievii P
NCBI: GCA_008329945.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	83.56	no
125438	anaerobic	anaerobicⓘ	yes	87.97	yes
125438	aerobic	aerobicⓘ	no	92.73	no
125438	spore-forming	spore-formingⓘ	no	73.83	no
125438	thermophilic	thermophileⓘ	no	83.73	yes
125438	flagellated	motile2+ⓘ	yes	68.99	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Description of Oceanispirochaeta crateris sp. nov. and reclassification of Spirochaeta perfilievii as Thiospirochaeta perfilievii gen. nov., comb. nov.	Dubinina G, Leshcheva N, Mikheeva N, Spring S, Neumann-Schaal M, Shcherbakova V, Grabovich M.	Int J Syst Evol Microbiol	10.1099/ijsem.0.004544	2020
Phylogeny	Spirochaeta perfilievii sp. nov., an oxygen-tolerant, sulfide-oxidizing, sulfur- and thiosulfate-reducing spirochaete isolated from a saline spring.	Dubinina G, Grabovich M, Leshcheva N, Rainey FA, Gavrish E	Int J Syst Evol Microbiol	10.1099/ijs.0.018333-0	2010

References

#7967	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 19205
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#26054	IJSEM 110 2011 ( DOI 10.1099/ijs.0.018333-0 , PubMed 20173011 )
#29667	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #26054
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68380	Automatically annotated from API rID32A .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Thiospirochaeta perfilievii (DSM 19205, VKM B-2514, P)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Observation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API rID32A

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_008329945

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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