Geobacillus thermoleovorans (DSM 5366, ATCC 43513, VKM B-2230)

Name: Geobacillus thermoleovorans (DSM 5366, ATCC 43513, VKM B-2230)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1433

Type strain

Strain Designation LEH-1, R-35636

Culture col. no. DSM 5366 ATCC 43513 VKM B-2230 LEH-1 R-35636

NCBI tax ID(s) 33941

Special Collections DSMZ Literature strains

History <- ATCC <- J.J. Perry, LEH-1

Links

Geobacillus thermoleovorans LEH-1 is a facultative anaerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from soil, near hot water effluent.

spore-forming Gram-positive rod-shaped colony-forming facultative anaerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 1207

LMG 9823,ATCC 43513,DSM 5366,NCIMB 12698,KCTC 3570,VKM B-2230,BCRC 17253,CCRC 17253

@ref 20215

Last LPSN update 2026-02-09 11:18:06

Domain Bacteria

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Geobacillus

Species Geobacillus thermoleovorans

Full scientific name Geobacillus thermoleovorans (Zarilla and Perry 1988) Nazina et al. 2001

Synonyms (1)

Bacillus thermoleovorans

BacDive ID	Other strains from **Geobacillus thermoleovorans** (3)	Type strain
1434	G. thermoleovorans HSR, DSM 11667
1435	G. thermoleovorans K-3d, DSM 11668
1436	G. thermoleovorans Fango, DSM 16053, ATCC BAA-898

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape
23043	positive	2.0-6.0 µm	0.7-1.5 µm	rod-shaped

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period
23043	0.5-5.0 mm	creamy white	circular	1 day

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
2006	NUTRIENT AGAR (DSMZ Medium 1)		Medium recipe at MediaDive	Name: NUTRIENT AGAR (DSMZ Medium 1) Composition: Agar 15.0 g/l Peptone 5.0 g/l Meat extract 3.0 g/l Distilled water
23043	trypticase soy agar

Culture temp

@ref	Growth	Type	Temperature (°C)
2006	positive	growth	60
23043	positive	growth	37.0-40.0
23043	positive	growth	65.0-70.0
23043	negative	growth	30.0
23043	negative	growth	80.0
23043	positive	optimum	60.0

Culture pH

@ref	Ability	Type	PH	PH range
23043	positive	optimum	6.0-7.0
23043	positive	growth	5.0
23043	positive	growth	9.0	alkaliphile

Physiology and metabolism

Oxygen tolerance

23043

Oxygen tolerancefacultative anaerobe

Spore formation

@ref	Spore description	Type of spore	Spore formation
23043	ellipsoidal,terminall and subterminal,do not swell the sporangia	endospore

Halophily

@ref	Salt	Growth	Tested relation	Concentration
23043	NaCl	positive	growth	0.5-1.0 %

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23043		1-o-methyl alpha-galactopyranoside	-	growth
23043	16808 ChEBI	2-dehydro-D-gluconate	-	builds acid from
23043	16808 ChEBI	2-dehydro-D-gluconate	-	growth
23043	30916 ChEBI	2-oxoglutarate	-	growth
23043	16193 ChEBI	3-hydroxybenzoate	-	growth
23043	37054 ChEBI	3-hydroxybutyrate	+	growth
23043		3-O-methyl alpha-D-glucopyranoside	-	growth
23043	51057 ChEBI	3-phenylpropionate	-	growth
23043		4-aminovalerate	-	growth
23043	17879 ChEBI	4-hydroxybenzoate	-	growth
23043	17426 ChEBI	5-dehydro-D-gluconate	-	builds acid from
23043	17426 ChEBI	5-dehydro-D-gluconate	-	growth
23043	27613 ChEBI	amygdalin	-	builds acid from
23043	18305 ChEBI	arbutin	-	builds acid from
23043	16150 ChEBI	benzoate	-	growth
23043	17750 ChEBI	betaine	-	growth
23043		casein	+	hydrolysis
23043	17057 ChEBI	cellobiose	-	builds acid from
23043	17057 ChEBI	cellobiose	+	growth
23043	16383 ChEBI	cis-aconitate	-	growth
23043	16947 ChEBI	citrate	-	carbon source
23043	16947 ChEBI	citrate	-	growth
23043	15570 ChEBI	D-alanine	+	growth
23043	17108 ChEBI	D-arabinose	-	builds acid from
23043	18333 ChEBI	D-arabitol	-	builds acid from
23043	18333 ChEBI	D-arabitol	-	growth
23043	15824 ChEBI	D-fructose	-	builds gas from
23043	15824 ChEBI	D-fructose	+	builds acid from
23043	15824 ChEBI	D-fructose	+	growth
23043	28847 ChEBI	D-fucose	-	builds acid from
23043	12936 ChEBI	D-galactose	+	growth
23043	18024 ChEBI	D-galacturonic acid	-	growth
23043	30612 ChEBI	D-glucarate	-	growth
23043	8391 ChEBI	D-gluconate	+	growth
23043	17315 ChEBI	D-glucosamine	+	growth
23043	17634 ChEBI	D-glucose	-	builds gas from
23043	17634 ChEBI	D-glucose	+	builds acid from
23043	17634 ChEBI	D-glucose	+	growth
23043	62318 ChEBI	D-lyxose	-	builds acid from
23043	62318 ChEBI	D-lyxose	-	growth
23043	15588 ChEBI	D-malate	-	growth
23043	16024 ChEBI	D-mannose	-	builds gas from
23043	16024 ChEBI	D-mannose	+	builds acid from
23043	16024 ChEBI	D-mannose	+	growth
23043	16988 ChEBI	D-ribose	+	growth
23043	17924 ChEBI	D-sorbitol	-	growth
23043	16443 ChEBI	D-tagatose	-	builds acid from
23043	16443 ChEBI	D-tagatose	-	growth
23043	30927 ChEBI	D-tartrate	-	growth
23043	65327 ChEBI	D-xylose	-	builds gas from
23043	65327 ChEBI	D-xylose	+	builds acid from
23043	65327 ChEBI	D-xylose	+	growth
23043	27689 ChEBI	decanoate	-	growth
23043	17113 ChEBI	erythritol	-	builds acid from
23043	17113 ChEBI	erythritol	-	growth
23043	4853 ChEBI	esculin	+	growth
23043	4853 ChEBI	esculin	+	hydrolysis
23043	16000 ChEBI	ethanolamine	-	growth
23043	29806 ChEBI	fumarate	+	growth
23043	16537 ChEBI	galactarate	-	growth
23043	16813 ChEBI	galactitol	-	builds acid from
23043	16813 ChEBI	galactitol	-	growth
23043	28260 ChEBI	galactose	-	builds gas from
23043	28260 ChEBI	galactose	+	builds acid from
23043	16865 ChEBI	gamma-aminobutyric acid	-	growth
23043	5291 ChEBI	gelatin	+	hydrolysis
23043	28066 ChEBI	gentiobiose	-	builds acid from
23043	28066 ChEBI	gentiobiose	+	growth
23043	58044 ChEBI	gentisate	-	growth
23043	24265 ChEBI	gluconate	-	builds acid from
23043	24297 ChEBI	glucuronate	-	growth
23043	17859 ChEBI	glutaric acid	-	growth
23043	33871 ChEBI	glycerate	+	growth
23043	17754 ChEBI	glycerol	-	builds gas from
23043	17754 ChEBI	glycerol	+	builds acid from
23043	17754 ChEBI	glycerol	+	growth
23043	28087 ChEBI	glycogen	-	builds gas from
23043	28087 ChEBI	glycogen	+	builds acid from
23043	18295 ChEBI	histamine	-	growth
23043	15443 ChEBI	inulin	-	builds acid from
23043	17240 ChEBI	itaconate	-	growth
23043	16977 ChEBI	L-alanine	+	growth
23043	30849 ChEBI	L-arabinose	-	builds acid from
23043	30849 ChEBI	L-arabinose	-	growth
23043	18403 ChEBI	L-arabitol	-	builds acid from
23043	18403 ChEBI	L-arabitol	-	growth
23043	29991 ChEBI	L-aspartate	-	growth
23043	18287 ChEBI	L-fucose	-	builds acid from
23043	18287 ChEBI	L-fucose	-	growth
23043	29985 ChEBI	L-glutamate	+	growth
23043	15971 ChEBI	L-histidine	+	growth
23043	15589 ChEBI	L-malate	+	growth
23043	17203 ChEBI	L-proline	+	growth
23043	62345 ChEBI	L-rhamnose	-	growth
23043	17115 ChEBI	L-serine	+	growth
23043	17266 ChEBI	L-sorbose	-	builds acid from
23043	17266 ChEBI	L-sorbose	-	growth
23043	30924 ChEBI	L-tartrate	-	growth
23043	16828 ChEBI	L-tryptophan	-	growth
23043	17895 ChEBI	L-tyrosine	-	growth
23043	65328 ChEBI	L-xylose	-	builds acid from
23043	24996 ChEBI	lactate	+	growth
23043	17716 ChEBI	lactose	-	builds acid from
23043	17716 ChEBI	lactose	+	growth
23043	6359 ChEBI	lactulose	+	growth
23043	32357 ChEBI	m-coumaric acid	-	growth
23043	15792 ChEBI	malonate	-	growth
23043	68428 ChEBI	maltitol	+	growth
23043	17306 ChEBI	maltose	-	builds gas from
23043	17306 ChEBI	maltose	+	builds acid from
23043	17306 ChEBI	maltose	+	growth
23043	61993 ChEBI	maltotriose	+	growth
23043	29864 ChEBI	mannitol	-	builds gas from
23043	29864 ChEBI	mannitol	+	builds acid from
23043	29864 ChEBI	mannitol	+	growth
23043	6731 ChEBI	melezitose	-	builds gas from
23043	6731 ChEBI	melezitose	+	builds acid from
23043	6731 ChEBI	melezitose	+	growth
23043	28053 ChEBI	melibiose	-	builds gas from
23043	28053 ChEBI	melibiose	+	builds acid from
23043	28053 ChEBI	melibiose	+	growth
23043	30928 ChEBI	meso-tartrate	-	growth
23043	320061 ChEBI	methyl alpha-D-glucopyranoside	-	growth
23043	320055 ChEBI	methyl beta-D-glucopyranoside	+	growth
23043	37657 ChEBI	methyl D-glucoside	-	builds acid from
23043	17268 ChEBI	myo-inositol	-	builds gas from
23043	17268 ChEBI	myo-inositol	+	builds acid from
23043	17268 ChEBI	myo-inositol	+	growth
23043	506227 ChEBI	N-acetylglucosamine	-	builds acid from
23043	506227 ChEBI	N-acetylglucosamine	+	growth
23043	17632 ChEBI	nitrate	+	reduction
23043		O-nitrophenyl-beta-D-galactopyranosid	-	hydrolysis
23043	25646 ChEBI	octanoate	-	growth
23043	18394 ChEBI	palatinose	+	growth
23043	18401 ChEBI	phenylacetate	+	growth
23043	17272 ChEBI	propionate	-	growth
23043	36241 ChEBI	protocatechuate	-	growth
23043	17148 ChEBI	putrescine	-	growth
23043	26490 ChEBI	quinate	+	growth
23043	16634 ChEBI	raffinose	-	builds gas from
23043	16634 ChEBI	raffinose	+	builds acid from
23043	16634 ChEBI	raffinose	+	growth
23043	26546 ChEBI	rhamnose	-	builds acid from
23043	15963 ChEBI	ribitol	-	builds acid from
23043	15963 ChEBI	ribitol	-	growth
23043	33942 ChEBI	ribose	-	builds gas from
23043	33942 ChEBI	ribose	+	builds acid from
23043	17814 ChEBI	salicin	-	builds gas from
23043	17814 ChEBI	salicin	+	builds acid from
23043	30911 ChEBI	sorbitol	-	builds acid from
23043	28017 ChEBI	starch	-	builds gas from
23043	28017 ChEBI	starch	+	builds acid from
23043	28017 ChEBI	starch	+	hydrolysis
23043	30031 ChEBI	succinate	+	growth
23043	17992 ChEBI	sucrose	-	builds gas from
23043	17992 ChEBI	sucrose	+	builds acid from
23043	17992 ChEBI	sucrose	+	growth
23043	15708 ChEBI	trans-aconitate	-	growth
23043	27082 ChEBI	trehalose	-	builds gas from
23043	27082 ChEBI	trehalose	+	builds acid from
23043	27082 ChEBI	trehalose	+	growth
23043	62517 ChEBI	tricarballylate	-	growth
23043	18123 ChEBI	trigonelline	-	growth
23043	16765 ChEBI	tryptamine	-	growth
23043	27897 ChEBI	tryptophan	-	energy source
23043	32528 ChEBI	turanose	-	builds acid from
23043	32528 ChEBI	turanose	+	growth
23043	17151 ChEBI	xylitol	-	builds acid from
23043	17151 ChEBI	xylitol	-	growth

Metabolite production

@ref	Chebi-ID	Metabolite
23043	15688 ChEBI	acetoin
23043	16136 ChEBI	hydrogen sulfide
23043	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Citrate test	Indole test
23043	15688 ChEBI	acetoin	+
23043	16947 ChEBI	citrate		-
23043	35581 ChEBI	indole			-

Enzymes

@ref	Value	Activity	Ec
23043	arginine dihydrolase	-	3.5.3.6
23043	beta-galactosidase	-	3.2.1.23
23043	catalase	+	1.11.1.6
23043	cytochrome oxidase	+	1.9.3.1
23043	lysine decarboxylase	-	4.1.1.18
23043	ornithine decarboxylase	-	4.1.1.17
23043	tryptophan deaminase	-	4.1.99.1
23043	urease	-	3.5.1.5

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	-
#Environmental	#Terrestrial	#Soil
#Condition	#Thermophilic (>45°C)	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
2006	soil, near hot water effluent	Bethlehem	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence Z26923 (>99% sequence identity) for Geobacillus from Microbeatlas

Aquatic Samples	635
Soil Samples	485
Animal Samples	2412
Plant Samples	182
Total Samples	3714

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
2006	1	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	NCBI tax ID
20218	Geobacillus thermoleovorans strain DSM 5366 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequence	EU157955	354	33941
20218	Geobacillus thermoleovorans isolate Gtl3 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723647	635	33941
20218	Geobacillus thermoleovorans isolate Gtl7 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723648	689	33941
20218	Geobacillus thermoleovorans isolate Gtl9 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723649	1025	33941
20218	Geobacillus thermoleovorans isolate Gtl12 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723650	689	33941
20218	Bacillus thermoleovorans (ATCC 43513) ribosomal RNA smaal subunit	M77488	1428	33941
2006	B.thermoleovorans gene for 16S ribosomal RNA	Z26923	1518	33941
2006	Geobacillus thermoleovorans partial 16S rRNA gene, strain R-35636	FN428684	1510	33941

GC content

@ref	GC-content (mol%)	Method
2006	52.4	high performance liquid chromatography (HPLC)
2006	58.0	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
	Efficient pathway-driven scyllo-inositol production from myo-inositol using thermophilic cells and mesophilic inositol dehydrogenases: a novel strategy for pathway control.	Kurashiki R, Takahashi M, Okumura Y, Ono T, Endo H, Makino K, Fukui K, Yokoyama K, Ishikawa S, Yoshida K-i, Ohshiro T, Suzuki H.	Appl Environ Microbiol	10.1128/aem.00281-24	2024
Stress	Influence of cations on growth of thermophilic Geobacillus spp. and Anoxybacillus flavithermus in planktonic culture.	Somerton B, Palmer J, Brooks J, Smolinski E, Lindsay D, Flint S.	Appl Environ Microbiol	10.1128/aem.06951-11	2012
Enzymology	Functional Characterization, Genome Assembly, and Annotation of Geobacillus sp. G4 Isolated from a Geothermal Field in Tacna, Peru.	Ticona ARP, Santos KCR, Ramirez-Arua HE, Castellanos R, Silva JP, Hamann PRV, Noronha EF, Lopes FAC.	Microorganisms	10.3390/microorganisms13061374	2025
Phylogeny	Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine.	Deflaun MF, Fredrickson JK, Dong H, Pfiffner SM, Onstott TC, Balkwill DL, Streger SH, Stackebrandt E, Knoessen S, van Heerden E	Syst Appl Microbiol	10.1016/j.syapm.2006.04.003	2006
Phylogeny	Isolation and characterization of long-chain-alkane degrading Bacillus thermoleovorans from deep subterranean petroleum reservoirs.	Kato T, Haruki M, Imanaka T, Morikawa M, Kanaya S	J Biosci Bioeng	10.1263/jbb.91.64	2001
Phylogeny	Geobacillus zalihae sp. nov., a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia.	Abd Rahman RN, Leow TC, Salleh AB, Basri M	BMC Microbiol	10.1186/1471-2180-7-77	2007
Phylogeny	Geobacillus thermoleovorans subsp. stromboliensis subsp. nov., isolated from the geothermal volcanic environment.	Romano I, Poli A, Lama L, Gambacorta A, Nicolaus B	J Gen Appl Microbiol	10.2323/jgam.51.183	2005
Phylogeny	Bacillus thermodenitrificans sp. nov., nom. rev.	Manachini PL, Mora D, Nicastro G, Parini C, Stackebrandt E, Pukall R, Fortina MG	Int J Syst Evol Microbiol	10.1099/00207713-50-3-1331	2000

References

#2006	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5366
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#20218	Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )
#23043	Anna E. Dinsdale,Gillian Halket,An Coorevits,Anita Van Landschoot,Hans-Jürgen Busse,Paul De Vos,Niall A. Logan: Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatus. IJSEM 61: 1802 - 1810 2011 ( DOI 10.1099/ijs.0.025445-0 , PubMed 20817844 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Geobacillus thermoleovorans (DSM 5366, ATCC 43513, VKM B-2230)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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