Name: Geobacillus thermoleovorans LEH-1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1433

Type strain:

Species: Geobacillus thermoleovorans

Strain Designation: LEH-1, R-35636

Culture col. no.: DSM 5366, ATCC 43513, VKM B-2230

Strain history: <- ATCC <- J.J. Perry, LEH-1

NCBI tax ID(s): 33941 (species)

SI-ID 1207

LMG 9823, ATCC 43513, DSM 5366, NCIMB 12698, KCTC 3570, VKM B-2230, BCRC 17253, CCRC 17253

Other strains from Geobacillus thermoleovorans

G. thermoleovorans HSR, DSM 11667
G. thermoleovorans K-3d, DSM 11668
G. thermoleovorans Fango, DSM 16053, ATCC BAA-898

Section

Geobacillus thermoleovorans LEH-1 is a facultative anaerobe, spore-forming, thermophilic bacterium that forms circular colonies and was isolated from soil, near hot water effluent.

colony-forming
Gram-positive
rod-shaped
spore-forming
facultative anaerobe
thermophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Geobacillus*
	Species	**Geobacillus thermoleovorans**
	Full Scientific Name (LPSN)	Geobacillus thermoleovorans (Zarilla and Perry 1988) Nazina et al. 2001

Synonym

Bacillus thermoleovorans

Information on morphological and physiological properties Morphology

[Ref.: #23043]	Gram stain	positive

[Ref.: #23043]	Cell length	2.0-6.0 µm

[Ref.: #23043]	Cell width	0.7-1.5 µm

[Ref.: #23043]	Cell shape	rod-shaped

[Ref.: #23043]	Colony size	0.5-5.0 mm
[Ref.: #23043]	Colony color	creamy white
[Ref.: #23043]	Colony shape	circular
[Ref.: #23043]	Incubation period	1 day

Information on culture and growth conditions Culture and growth conditions

[Ref.: #2006]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #2006]

Culture medium growth

[Ref.: #2006]

Culture medium link

Medium recipe at MediaDive

[Ref.: #2006]

Culture medium composition

expand / minimize

[Ref.: #23043]

Culture medium

trypticase soy agar

[Ref.: #23043]

Culture medium growth

	Kind of temperature		Temperature
[Ref.: #2006]		growth	60 °C
[Ref.: #23043]		growth	37.0-40.0 °C
[Ref.: #23043]		growth	65.0-70.0 °C
[Ref.: #23043]	no	growth	30.0 °C
[Ref.: #23043]	no	growth	80.0 °C
[Ref.: #23043]		optimum	60.0 °C

[Ref.: #2006]	Temperature range	thermophilic
[Ref.: #23043]	Temperature range	thermophilic
[Ref.: #23043]	Temperature range	mesophilic
[Ref.: #23043]	Temperature range	hyperthermophilic
[Ref.: #23043]	Temperature range	thermophilic

		pH
[Ref.: #23043]	optimum	6.0-7.0
[Ref.: #23043]	growth	5.0
[Ref.: #23043]	growth	9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #23043]

Oxygen tolerance

facultative anaerobe

[Ref.: #23043]	Spore description	ellipsoidal,terminall and subterminal,do not swell the sporangia
[Ref.: #23043]	Type of spore	endospore
[Ref.: #23043]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #23043]		NaCl		growth	0.5-1.0	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #23043]	1-o-methyl alpha-galactopyranoside	-	growth
[Ref.: #23043]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23043]	2-dehydro-D-gluconate ChEBI	-	growth
[Ref.: #23043]	2-oxoglutarate ChEBI	-	growth
[Ref.: #23043]	3-hydroxybenzoate ChEBI	-	growth
[Ref.: #23043]	3-hydroxybutyrate ChEBI	+	growth
[Ref.: #23043]	3-O-methyl alpha-D-glucopyranoside	-	growth
[Ref.: #23043]	3-phenylpropionate ChEBI	-	growth
[Ref.: #23043]	4-aminovalerate	-	growth
[Ref.: #23043]	4-hydroxybenzoate ChEBI	-	growth
[Ref.: #23043]	5-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #23043]	5-dehydro-D-gluconate ChEBI	-	growth
[Ref.: #23043]	amygdalin ChEBI	-	builds acid from
[Ref.: #23043]	arbutin ChEBI	-	builds acid from
[Ref.: #23043]	benzoate ChEBI	-	growth
[Ref.: #23043]	betaine ChEBI	-	growth
[Ref.: #23043]	casein	+	hydrolysis
[Ref.: #23043]	cellobiose ChEBI	-	builds acid from
[Ref.: #23043]	cellobiose ChEBI	+	growth
[Ref.: #23043]	cis-aconitate ChEBI	-	growth
[Ref.: #23043]	citrate ChEBI	-	carbon source
[Ref.: #23043]	citrate ChEBI	-	growth
[Ref.: #23043]	D-alanine ChEBI	+	growth
[Ref.: #23043]	D-arabinose ChEBI	-	builds acid from
[Ref.: #23043]	D-arabitol ChEBI	-	builds acid from
[Ref.: #23043]	D-arabitol ChEBI	-	growth
[Ref.: #23043]	D-fructose ChEBI	-	builds gas from
[Ref.: #23043]	D-fructose ChEBI	+	builds acid from
[Ref.: #23043]	D-fructose ChEBI	+	growth
[Ref.: #23043]	D-fucose ChEBI	-	builds acid from
[Ref.: #23043]	D-galactose ChEBI	+	growth
[Ref.: #23043]	D-galacturonic acid ChEBI	-	growth
[Ref.: #23043]	D-glucarate ChEBI	-	growth
[Ref.: #23043]	D-gluconate ChEBI	+	growth
[Ref.: #23043]	D-glucosamine ChEBI	+	growth
[Ref.: #23043]	D-glucose ChEBI	-	builds gas from
[Ref.: #23043]	D-glucose ChEBI	+	builds acid from
[Ref.: #23043]	D-glucose ChEBI	+	growth
[Ref.: #23043]	D-lyxose ChEBI	-	builds acid from
[Ref.: #23043]	D-lyxose ChEBI	-	growth
[Ref.: #23043]	D-malate ChEBI	-	growth
[Ref.: #23043]	D-mannose ChEBI	-	builds gas from
[Ref.: #23043]	D-mannose ChEBI	+	builds acid from
[Ref.: #23043]	D-mannose ChEBI	+	growth
[Ref.: #23043]	D-ribose ChEBI	+	growth
[Ref.: #23043]	D-sorbitol ChEBI	-	growth
[Ref.: #23043]	D-tagatose ChEBI	-	builds acid from
[Ref.: #23043]	D-tagatose ChEBI	-	growth
[Ref.: #23043]	D-tartrate ChEBI	-	growth
[Ref.: #23043]	D-xylose ChEBI	-	builds gas from
[Ref.: #23043]	D-xylose ChEBI	+	builds acid from
[Ref.: #23043]	D-xylose ChEBI	+	growth
[Ref.: #23043]	decanoate ChEBI	-	growth
[Ref.: #23043]	erythritol ChEBI	-	builds acid from
[Ref.: #23043]	erythritol ChEBI	-	growth
[Ref.: #23043]	esculin ChEBI	+	growth
[Ref.: #23043]	esculin ChEBI	+	hydrolysis
[Ref.: #23043]	ethanolamine ChEBI	-	growth
[Ref.: #23043]	fumarate ChEBI	+	growth
[Ref.: #23043]	galactarate ChEBI	-	growth
[Ref.: #23043]	galactitol ChEBI	-	builds acid from
[Ref.: #23043]	galactitol ChEBI	-	growth
[Ref.: #23043]	galactose ChEBI	-	builds gas from
[Ref.: #23043]	galactose ChEBI	+	builds acid from
[Ref.: #23043]	gamma-aminobutyric acid ChEBI	-	growth
[Ref.: #23043]	gelatin ChEBI	+	hydrolysis
[Ref.: #23043]	gentiobiose ChEBI	-	builds acid from
[Ref.: #23043]	gentiobiose ChEBI	+	growth
[Ref.: #23043]	gentisate ChEBI	-	growth
[Ref.: #23043]	gluconate ChEBI	-	builds acid from
[Ref.: #23043]	glucuronate ChEBI	-	growth
[Ref.: #23043]	glutaric acid ChEBI	-	growth
[Ref.: #23043]	glycerate ChEBI	+	growth
[Ref.: #23043]	glycerol ChEBI	-	builds gas from
[Ref.: #23043]	glycerol ChEBI	+	builds acid from
[Ref.: #23043]	glycerol ChEBI	+	growth
[Ref.: #23043]	glycogen ChEBI	-	builds gas from
[Ref.: #23043]	glycogen ChEBI	+	builds acid from
[Ref.: #23043]	histamine ChEBI	-	growth
[Ref.: #23043]	inulin ChEBI	-	builds acid from
[Ref.: #23043]	itaconate ChEBI	-	growth
[Ref.: #23043]	L-alanine ChEBI	+	growth
[Ref.: #23043]	L-arabinose ChEBI	-	builds acid from
[Ref.: #23043]	L-arabinose ChEBI	-	growth
[Ref.: #23043]	L-arabitol ChEBI	-	builds acid from
[Ref.: #23043]	L-arabitol ChEBI	-	growth
[Ref.: #23043]	L-aspartate ChEBI	-	growth
[Ref.: #23043]	L-fucose ChEBI	-	builds acid from
[Ref.: #23043]	L-fucose ChEBI	-	growth
[Ref.: #23043]	L-glutamate ChEBI	+	growth
[Ref.: #23043]	L-histidine ChEBI	+	growth
[Ref.: #23043]	L-malate ChEBI	+	growth
[Ref.: #23043]	L-proline ChEBI	+	growth
[Ref.: #23043]	L-rhamnose ChEBI	-	growth
[Ref.: #23043]	L-serine ChEBI	+	growth
[Ref.: #23043]	L-sorbose ChEBI	-	builds acid from
[Ref.: #23043]	L-sorbose ChEBI	-	growth
[Ref.: #23043]	L-tartrate ChEBI	-	growth
[Ref.: #23043]	L-tryptophan ChEBI	-	growth
[Ref.: #23043]	L-tyrosine ChEBI	-	growth
[Ref.: #23043]	L-xylose ChEBI	-	builds acid from
[Ref.: #23043]	lactate ChEBI	+	growth
[Ref.: #23043]	lactose ChEBI	-	builds acid from
[Ref.: #23043]	lactose ChEBI	+	growth
[Ref.: #23043]	lactulose ChEBI	+	growth
[Ref.: #23043]	m-coumaric acid ChEBI	-	growth
[Ref.: #23043]	malonate ChEBI	-	growth
[Ref.: #23043]	maltitol ChEBI	+	growth
[Ref.: #23043]	maltose ChEBI	-	builds gas from
[Ref.: #23043]	maltose ChEBI	+	builds acid from
[Ref.: #23043]	maltose ChEBI	+	growth
[Ref.: #23043]	maltotriose ChEBI	+	growth
[Ref.: #23043]	mannitol ChEBI	-	builds gas from
[Ref.: #23043]	mannitol ChEBI	+	builds acid from
[Ref.: #23043]	mannitol ChEBI	+	growth
[Ref.: #23043]	melezitose ChEBI	-	builds gas from
[Ref.: #23043]	melezitose ChEBI	+	builds acid from
[Ref.: #23043]	melezitose ChEBI	+	growth
[Ref.: #23043]	melibiose ChEBI	-	builds gas from
[Ref.: #23043]	melibiose ChEBI	+	builds acid from
[Ref.: #23043]	melibiose ChEBI	+	growth
[Ref.: #23043]	meso-tartrate ChEBI	-	growth
[Ref.: #23043]	methyl alpha-D-glucopyranoside ChEBI	-	growth
[Ref.: #23043]	methyl beta-D-glucopyranoside ChEBI	+	growth
[Ref.: #23043]	methyl D-glucoside ChEBI	-	builds acid from
[Ref.: #23043]	myo-inositol ChEBI	-	builds gas from
[Ref.: #23043]	myo-inositol ChEBI	+	builds acid from
[Ref.: #23043]	myo-inositol ChEBI	+	growth
[Ref.: #23043]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #23043]	N-acetylglucosamine ChEBI	+	growth
[Ref.: #23043]	nitrate ChEBI	+	reduction
[Ref.: #23043]	O-nitrophenyl-beta-D-galactopyranosid	-	hydrolysis
[Ref.: #23043]	octanoate ChEBI	-	growth
[Ref.: #23043]	palatinose ChEBI	+	growth
[Ref.: #23043]	phenylacetate ChEBI	+	growth
[Ref.: #23043]	propionate ChEBI	-	growth
[Ref.: #23043]	protocatechuate ChEBI	-	growth
[Ref.: #23043]	putrescine ChEBI	-	growth
[Ref.: #23043]	quinate ChEBI	+	growth
[Ref.: #23043]	raffinose ChEBI	-	builds gas from
[Ref.: #23043]	raffinose ChEBI	+	builds acid from
[Ref.: #23043]	raffinose ChEBI	+	growth
[Ref.: #23043]	rhamnose ChEBI	-	builds acid from
[Ref.: #23043]	ribitol ChEBI	-	builds acid from
[Ref.: #23043]	ribitol ChEBI	-	growth
[Ref.: #23043]	ribose ChEBI	-	builds gas from
[Ref.: #23043]	ribose ChEBI	+	builds acid from
[Ref.: #23043]	salicin ChEBI	-	builds gas from
[Ref.: #23043]	salicin ChEBI	+	builds acid from
[Ref.: #23043]	sorbitol ChEBI	-	builds acid from
[Ref.: #23043]	starch ChEBI	-	builds gas from
[Ref.: #23043]	starch ChEBI	+	builds acid from
[Ref.: #23043]	starch ChEBI	+	hydrolysis
[Ref.: #23043]	succinate ChEBI	+	growth
[Ref.: #23043]	sucrose ChEBI	-	builds gas from
[Ref.: #23043]	sucrose ChEBI	+	builds acid from
[Ref.: #23043]	sucrose ChEBI	+	growth
[Ref.: #23043]	trans-aconitate ChEBI	-	growth
[Ref.: #23043]	trehalose ChEBI	-	builds gas from
[Ref.: #23043]	trehalose ChEBI	+	builds acid from
[Ref.: #23043]	trehalose ChEBI	+	growth
[Ref.: #23043]	tricarballylate ChEBI	-	growth
[Ref.: #23043]	trigonelline ChEBI	-	growth
[Ref.: #23043]	tryptamine ChEBI	-	growth
[Ref.: #23043]	tryptophan ChEBI	-	energy source
[Ref.: #23043]	turanose ChEBI	-	builds acid from
[Ref.: #23043]	turanose ChEBI	+	growth
[Ref.: #23043]	xylitol ChEBI	-	builds acid from
[Ref.: #23043]	xylitol ChEBI	-	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #23043]	acetoin ChEBI	yes
[Ref.: #23043]	hydrogen sulfide ChEBI	no
[Ref.: #23043]	indole ChEBI	no

	Metabolite	Voges-Proskauer-test	Indole test	Citrate test
[Ref.: #23043]	acetoin ChEBI	+
[Ref.: #23043]	citrate ChEBI			-
[Ref.: #23043]	indole ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #23043]	arginine dihydrolase	-	3.5.3.6
[Ref.: #23043]	beta-galactosidase	-	3.2.1.23
[Ref.: #23043]	catalase	+	1.11.1.6
[Ref.: #23043]	cytochrome oxidase	+	1.9.3.1
[Ref.: #23043]	lysine decarboxylase	-	4.1.1.18
[Ref.: #23043]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #23043]	tryptophan deaminase	-	4.1.99.1
[Ref.: #23043]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #2006]	Sample type/isolated from	soil, near hot water effluent
[Ref.: #2006]	Geographic location (country and/or sea, region)	Bethlehem
[Ref.: #2006]	Country	USA
[Ref.: #2006]	Country ISO 3 Code	USA
[Ref.: #2006]	Continent	North America
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	-
#Environmental	#Terrestrial	#Soil
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Z26923 (>99% sequence identity) for Geobacillus from Microbeatlas

Aquatic Samples	635
Soil Samples	485
Animal Samples	2412
Plant Samples	182
Total Samples	3714

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #2006]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #2006]	Geobacillus thermoleovorans partial 16S rRNA gene, strain R-35636	FN428684	1510	ENA	33941 tax ID
[Ref.: #2006]	B.thermoleovorans gene for 16S ribosomal RNA	Z26923	1518	ENA	33941 tax ID
[Ref.: #20218]	Geobacillus thermoleovorans strain DSM 5366 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequence	EU157955	354	ENA	33941 tax ID	*
[Ref.: #20218]	Bacillus thermoleovorans (ATCC 43513) ribosomal RNA smaal subunit	M77488	1428	ENA	33941 tax ID	*
[Ref.: #20218]	Geobacillus thermoleovorans isolate Gtl3 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723647	635	ENA	33941 tax ID	*
[Ref.: #20218]	Geobacillus thermoleovorans isolate Gtl7 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723648	689	ENA	33941 tax ID	*
[Ref.: #20218]	Geobacillus thermoleovorans isolate Gtl9 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723649	1025	ENA	33941 tax ID	*
[Ref.: #20218]	Geobacillus thermoleovorans isolate Gtl12 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723650	689	ENA	33941 tax ID	*

[Ref.: #2006]	GC-content	52.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #2006]	GC-content	58.0 mol%	thermal denaturation, midpoint method (Tm)

Availability in culture collections External links

[Ref.: #2006]

Culture collection no.

DSM 5366, ATCC 43513, VKM B-2230

[Ref.: #71083]

SI-ID 1207

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Geobacillus zalihae sp. nov., a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia.	Abd Rahman RN, Leow TC, Salleh AB, Basri M	BMC Microbiol	10.1186/1471-2180-7-77	2007	*
Phylogeny	Isolation and characterization of a Geobacillus thermoleovorans strain from an ultra-deep South African gold mine.	Deflaun MF, Fredrickson JK, Dong H, Pfiffner SM, Onstott TC, Balkwill DL, Streger SH, Stackebrandt E, Knoessen S, van Heerden E	Syst Appl Microbiol	10.1016/j.syapm.2006.04.003	2006	*
Phylogeny	Isolation and characterization of long-chain-alkane degrading Bacillus thermoleovorans from deep subterranean petroleum reservoirs.	Kato T, Haruki M, Imanaka T, Morikawa M, Kanaya S	J Biosci Bioeng	10.1263/jbb.91.64	2001	*
Phylogeny	Geobacillus thermoleovorans subsp. stromboliensis subsp. nov., isolated from the geothermal volcanic environment.	Romano I, Poli A, Lama L, Gambacorta A, Nicolaus B	J Gen Appl Microbiol	10.2323/jgam.51.183	2005	*
Phylogeny	Bacillus thermodenitrificans sp. nov., nom. rev.	Manachini PL, Mora D, Nicastro G, Parini C, Stackebrandt E, Pukall R, Fortina MG	Int J Syst Evol Microbiol	10.1099/00207713-50-3-1331	2000	*

References

#2006

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 5366

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#23043

Anna E. Dinsdale,Gillian Halket,An Coorevits,Anita Van Landschoot,Hans-Jürgen Busse,Paul De Vos,Niall A. Logan: Emended descriptions of Geobacillus thermoleovorans and Geobacillus thermocatenulatus. IJSEM 61: 1802 - 1810 2011 ( DOI 10.1099/ijs.0.025445-0 , PubMed 20817844 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#71083

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID1207.1 )

* These data were automatically processed and therefore are not curated

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