Alkalispirochaeta alkalica (DSM 8900, ATCC 700262, Z-7491)

Name: Alkalispirochaeta alkalica (DSM 8900, ATCC 700262, Z-7491)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14327

Type strain

Strain Designation Z-7491

Culture col. no. DSM 8900 ATCC 700262 Z-7491

NCBI tax ID(s) 942938 46356

Special Collections DSMZ

Links

Alkalispirochaeta alkalica Z-7491 is a mesophilic prokaryote that was isolated from cyanobacterial mat from a warm spring in an alkaline lake.

mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 43851

ATCC 700262,DSM 8900

@ref 20215

Last LPSN update 2025-12-16 09:50:19

Domain Pseudomonadati

Phylum Spirochaetota

Class Spirochaetia

Order Spirochaetales

Family Spirochaetaceae

Genus Alkalispirochaeta

Species Alkalispirochaeta alkalica

Full scientific name Alkalispirochaeta alkalica (Zhilina et al. 1996) Sravanthi et al. 2016

Synonyms (1)

Spirochaeta alkalica

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	95.1

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
3434	ALKALIPHILIC SPIROCHAETE MEDIUM (DSMZ Medium 700)		Medium recipe at MediaDive	Name: ALKALIPHILIC SPIROCHAETE MEDIUM (DSMZ Medium 700) Composition: NaHCO3 15.0 g/l Na2CO3 10.0 g/l Sucrose 5.0 g/l Na2S x 9 H2O 1.0 g/l NH4Cl 1.0 g/l Yeast extract 0.5 g/l K2HPO4 0.2 g/l KCl 0.2 g/l MgSO4 x 7 H2O 0.003 g/l Nitrilotriacetic acid 0.0015 g/l NaCl 0.001 g/l MnSO4 x H2O 0.0005 g/l CoSO4 x 7 H2O 0.00018 g/l ZnSO4 x 7 H2O 0.00018 g/l FeSO4 x 7 H2O 0.0001 g/l Pyridoxine hydrochloride 0.0001 g/l CaCl2 x 2 H2O 0.0001 g/l Calcium D-(+)-pantothenate 5e-05 g/l (DL)-alpha-Lipoic acid 5e-05 g/l Nicotinic acid 5e-05 g/l Riboflavin 5e-05 g/l Thiamine HCl 5e-05 g/l p-Aminobenzoic acid 5e-05 g/l NiCl2 x 6 H2O 3e-05 g/l Biotin 2e-05 g/l Folic acid 2e-05 g/l AlK(SO4)2 x 12 H2O 2e-05 g/l CuSO4 x 5 H2O 1e-05 g/l H3BO3 1e-05 g/l Na2MoO4 x 2 H2O 1e-05 g/l Vitamin B12 1e-06 g/l Na2WO4 x 2 H2O 4e-07 g/l Na2SeO3 x 5 H2O 3e-07 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
3434	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	anaerobe	99.8

Spore formation

@ref	Spore formation	Confidence
125439		91.9

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Condition	#Alkaline	-
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Microbial community	#Microbial mat
#Host	#Microbial	#Bacteria

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
3434	cyanobacterial mat from a warm spring in an alkaline lake	Lake Magadi	Kenya	KEN	Africa

Taxonmaps

69479

Global distribution of 16S sequence X93927 (>99% sequence identity) for Alkalispirochaeta from Microbeatlas

Aquatic Samples	304
Soil Samples	26
Animal Samples	52
Plant Samples
Total Samples	382

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
3434	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM37354v1 assembly for Alkalispirochaeta alkalica DSM 8900	scaffold	GCA_000373545	942938.12	2515154091	942938	59.36
66792	Spirochaeta alkalica DSM 8900	contig		942938.3		942938	16.9

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
3434	S.alkalica 16S ribosomal RNA	X93927	1475		942938

GC content

@ref	GC-content (mol%)	Method
3434	57.1	thermal denaturation, midpoint method (Tm)

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Alkalispirochaeta alkalica DSM 8900
NCBI: GCA_000373545

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	91.90	no
125439	motility	BacteriaNetⓘ	yes	66.20	no
125439	gram_stain	BacteriaNetⓘ	negative	95.10	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	99.80	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Alkalispirochaeta alkalica DSM 8900 DSM 8900
NCBI: GCA_000373545.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	84.46	no
125438	anaerobic	anaerobicⓘ	yes	79.24	no
125438	aerobic	aerobicⓘ	no	86.24	no
125438	spore-forming	spore-formingⓘ	no	78.84	no
125438	thermophilic	thermophileⓘ	no	82.05	yes
125438	flagellated	motile2+ⓘ	yes	59.64	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Genetics	Analysis of 1,000+ Type-Strain Genomes Substantially Improves Taxonomic Classification of Alphaproteobacteria.	Hordt A, Lopez MG, Meier-Kolthoff JP, Schleuning M, Weinhold LM, Tindall BJ, Gronow S, Kyrpides NC, Woyke T, Goker M.	Front Microbiol	10.3389/fmicb.2020.00468	2020
Phylogeny	Alkalispirochaeta cellulosivorans gen. nov., sp. nov., a cellulose-hydrolysing, alkaliphilic, halotolerant bacterium isolated from the gut of a wood-eating cockroach (Cryptocercus punctulatus), and reclassification of four species of Spirochaeta as new combinations within Alkalispirochaeta gen. nov.	Sravanthi T, Tushar L, Sasikala C, Ramana CV	Int J Syst Evol Microbiol	10.1099/ijsem.0.000865	2015
Phylogeny	Spirochaeta odontotermitis sp. nov., an obligately anaerobic, cellulolytic, halotolerant, alkaliphilic spirochaete isolated from the termite Odontotermes obesus (Rambur) gut.	Sravanthi T, Tushar L, Sasikala C, Ramana CV	Int J Syst Evol Microbiol	10.1099/ijsem.0.000616	2015
Phylogeny	Spirochaeta sphaeroplastigenens sp. nov., a halo-alkaliphilic, obligately anaerobic spirochaete isolated from soda lake Lonar.	Vishnuvardhan Reddy S, Aspana S, Tushar DL, Sasikala C, Ramana CV	Int J Syst Evol Microbiol	10.1099/ijs.0.046292-0	2012
Phylogeny	Spirochaeta americana sp. nov., a new haloalkaliphilic, obligately anaerobic spirochaete isolated from soda Mono Lake in California.	Hoover RB, Pikuta EV, Bej AK, Marsic D, Whitman WB, Tang J, Krader P	Int J Syst Evol Microbiol	10.1099/ijs.0.02535-0	2003
Phylogeny	Spirochaeta alkalica sp. nov., Spirochaeta africana sp. nov., and Spirochaeta asiatica sp. nov., alkaliphilic anaerobes from the Continental Soda Lakes in Central Asia and the East African Rift.	Zhilina TN, Zavarzin GA, Rainey F, Kevbrin VV, Kostrikina NA, Lysenko AM	Int J Syst Bacteriol	10.1099/00207713-46-1-305	1996

References

#3434	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 8900
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Alkalispirochaeta alkalica (DSM 8900, ATCC 700262, Z-7491)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000373545

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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