Borrelia bissettiae (DSM 17990, CIP 109136, DN127)

Name: Borrelia bissettiae (DSM 17990, CIP 109136, DN127)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14311

Type strain

Strain Designation DN127, DN 127

Culture col. no. DSM 17990 CIP 109136 DN127 DN 127

NCBI tax ID(s) 64897

Special Collections DSMZ CIP Literature strains

Links

Borrelia bissettiae DN127 is a microaerophile, mesophilic, motile prokaryote that was isolated from tick Ixodes pacificus.

motile helical-shaped microaerophile mesophilic 16S sequence

Name and taxonomic classification

SI-ID 361800

DSM 17990,CIP 109136

@ref 20215

Last LPSN update 2025-12-16 09:50:19

Domain Pseudomonadati

Phylum Spirochaetota

Class Spirochaetia

Order Spirochaetales

Family Borreliaceae

Genus Borrelia

Species Borrelia bissettiae

Full scientific name Borrelia bissettiae Margos et al. 2016

Synonyms (2)

Borreliella bissettiae
"Borrelia bissettii"

Morphology

Cell morphology

@ref	Cell length	Cell width	Cell shape	Motility
43710	20 µm	0.2 µm	helical-shaped

Colony morphology

7320

Incubation period3-7 days

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
7320	BSK-MEDIUM (DSMZ Medium 403)	Medium recipe at MediaDive	Name: BSK-MEDIUM (DSMZ Medium 403) Composition: Bovine serum albumin 35.0245 g/l Gelatine 9.79706 g/l HEPES buffer 4.19874 g/l Proteose peptone no. 2 3.49895 g/l Glucose 2.09937 g/l Proteose yeastolate 0.69979 g/l Tryptone peptone 0.69979 g/l Na-pyruvate 0.559832 g/l Sodium citrate 0.489853 g/l N-Acetylglucosamine 0.279916 g/l MgCl2 x 6 H2O 0.209937 g/l L-Glutamine 0.069979 g/l Double distilled water CMRL 1066 Rabbit serum
34354	MEDIUM 83 - for Borrelia		BSK-H medium (1000.000 ml)
116823	CIP Medium 83	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
7320	positive	growth	35	mesophilic
34354	positive	growth	34	mesophilic
43710	positive	optimum	33-34	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
7320	microaerophile
43710	microaerophile

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Tick

Isolation

@ref	Sample type	Host species	Geographic location	Country	Country ISO 3 Code	Continent
7320	tick Ixodes pacificus	Ixodes pacificus	California	USA	USA	North America
43710	A questing Ixodes pacificus tick collected in Del Norte County		Del Norte County, California	USA	USA	North America
116823	Other, Ixodes pacificus		California	United States of America	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence AJ224141 (>99% sequence identity) for Borreliella from Microbeatlas

Aquatic Samples	378
Soil Samples	126
Animal Samples	1133
Plant Samples	40
Total Samples	1677

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
116823	2	Risk group (French classification) According to TRBA 466
7320	2	Risk group (German classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
7320	Borrelia burgdorferi sensu lato, 16S rRNA gene, isolate DN127	AJ224141	1467		521010

GC content

7320

GC-content (mol%)27

Genome-based predictions

Literature

Title	Authors	Journal	DOI	Year
Distinction between Borrelia and Borreliella is more robustly supported by molecular and phenotypic characteristics than all other neighbouring prokaryotic genera: Response to Margos' et al. "The genus Borrelia reloaded" (PLoS ONE 13(12): e0208432).	Gupta RS.	PLoS One	10.1371/journal.pone.0221397	2019
The genus Borrelia reloaded.	Margos G, Gofton A, Wibberg D, Dangel A, Marosevic D, Loh SM, Oskam C, Fingerle V.	PLoS One	10.1371/journal.pone.0208432	2018
Comparative study of binding of ovine complement factor H with different Borrelia genospecies.	Kisova-Vargova L, Cernanska D, Bhide M	Folia Microbiol (Praha)	10.1007/s12223-012-0104-y	2012
Whole-genome sequences of Borrelia bissettii, Borrelia valaisiana, and Borrelia spielmanii.	Schutzer SE, Fraser-Liggett CM, Qiu WG, Kraiczy P, Mongodin EF, Dunn JJ, Luft BJ, Casjens SR	J Bacteriol	10.1128/JB.06263-11	2012
Multilocus sequence analysis of Borrelia bissettii strains from North America reveals a new Borrelia species, Borrelia kurtenbachii.	Margos G, Hojgaard A, Lane RS, Cornet M, Fingerle V, Rudenko N, Ogden N, Aanensen DM, Fish D, Piesman J	Ticks Tick Borne Dis	10.1016/j.ttbdis.2010.09.002	2010
Detection and characterization of Borrelia bissettii in rodents from the central California coast.	Vredevoe LK, Stevens JR, Schneider BS	J Med Entomol	10.1603/0022-2585-41.4.736	2004
Bacteriolytic activity of selected vertebrate sera for Borrelia burgdorferi sensu stricto and Borrelia bissettii.	Ullmann AJ, Lane RS, Kurtenbach K, Miller M, Schriefer ME, Zeldner N, Piesman J	J Parasitol	10.1645/Ge-3081RN	2003
Molecular characterization of Borrelia spp. isolates from greater metropolitan Chicago reveals the presence of Borrelia bissettii. Preliminary report.	Picken RN, Picken MM	J Mol Microbiol Biotechnol		2000
Borrelia isolates in Northern Colorado identified as Borrelia bissettii.	Schneider BS, Zeidner NS, Burkot TR, Maupin GO, Piesman J	J Clin Microbiol	10.1128/JCM.38.8.3103-3105.2000	2000
Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov. prevail in diverse enzootic transmission cycles.	Margos G, Lane RS, Fedorova N, Koloczek J, Piesman J, Hojgaard A, Sing A, Fingerle V	Int J Syst Evol Microbiol	10.1099/ijsem.0.000897	2016
Expanded diversity among Californian borrelia isolates and description of Borrelia bissettii sp. nov. (formerly Borrelia group DN127).	Postic D, Ras NM, Lane RS, Hendson M, Baranton G	J Clin Microbiol	10.1128/JCM.36.12.3497-3504.1998	1998

References

#7320	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 17990
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#34354	; Curators of the CIP;
#43710	Gabriele Margos, Robert S. Lane, Natalia Fedorova, Johannes Koloczek, Joseph Piesman, Andrias Hojgaard, Andreas Sing, Volker Fingerle: Borrelia bissettiae sp. nov. and Borrelia californiensis sp. nov. prevail in diverse enzootic transmission cycles. IJSEM 66: 1447 - 1452 2016 ( DOI 10.1099/ijsem.0.000897 , PubMed 26813420 )
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#116823	Collection of Institut Pasteur ; Curators of the CIP; CIP 109136
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Borrelia bissettiae (DSM 17990, CIP 109136, DN127)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

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