Name: Geobacillus thermodenitrificans L 2-65
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 1424

Type strain:

Species: Geobacillus thermodenitrificans

Strain Designation: L 2-65, R-35647

Culture col. no.: DSM 465, ATCC 29492, BGSC 94A1, LMG 17532

Strain history: <- F. Hollaus, L 2-65 (Bacillus thermodenitrificans)

NCBI tax ID(s): 1413215 (strain), 33940 (species)

SI-ID 14062

LMG 17532, ATCC 29492, CCM 2565, DSM 465, NCIMB 11730, BCRC 11733, CCRC 11733, KCTC 3902, KACC 11363

Other strains from Geobacillus thermodenitrificans

G. thermodenitrificans CCUG 39164
G. thermodenitrificans CCUG 44674
G. thermodenitrificans CCUG 44679
G. thermodenitrificans CCUG 45409
G. thermodenitrificans JCM 32406

Section

Geobacillus thermodenitrificans L 2-65 is a thermophilic bacterium that was isolated from sugar beet juice from extraction installations.

thermophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Geobacillus*
	Species	**Geobacillus thermodenitrificans**
	Full Scientific Name (LPSN)	Geobacillus thermodenitrificans (Manachini et al. 2000 ex Klaushofer and Hollaus 1970) Nazina et al. 2001

Synonym

Bacillus thermodenitrificans

Information on culture and growth conditions Culture and growth conditions

[Ref.: #118]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #118]

Culture medium growth

[Ref.: #118]

Culture medium link

Medium recipe at MediaDive

[Ref.: #118]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #118]			growth	60 °C

[Ref.: #118]

Temperature range

thermophilic

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #118]	Sample type/isolated from	sugar beet juice from extraction installations
[Ref.: #118]	Country	Austria
[Ref.: #118]	Country ISO 3 Code	AUT
[Ref.: #118]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)
#Host Body Product	#Plant	#Juice (natural)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AY608960 (>99% sequence identity) for Geobacillus from Microbeatlas

Aquatic Samples	1277
Soil Samples	2618
Animal Samples	3638
Plant Samples	757
Total Samples	8290

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #118]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

Only first 5 entries are displayed. Click here to see all.

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #118]	Geobacillus thermodenitrificans strain BGSC 94A1 16S ribosomal RNA gene, complete sequence	AY608960	1560	ENA	33940 tax ID
[Ref.: #118]	Geobacillus thermodenitrificans partial 16S rRNA gene, strain R-35647	FN538993	1509	ENA	33940 tax ID
[Ref.: #20218]	Geobacillus thermodenitrificans strain DSM 465 16S-23S ribosomal RNA intergenic spacer and tRNA-Ile gene, partial sequence; and tRNA-Ala gene, complete sequence	EU157953	291	ENA	1413215 tax ID	*
[Ref.: #20218]	B.thermodenitrificans gene for 16S ribosomal RNA	Z26928	1517	ENA	1413215 tax ID	*
[Ref.: #20218]	Geobacillus thermodenitrificans isolate Gth6 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723641	507	ENA	1413215 tax ID	*
[Ref.: #20218]	Geobacillus thermodenitrificans isolate Gth8 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723642	621	ENA	1413215 tax ID	*
[Ref.: #20218]	Geobacillus thermodenitrificans isolate Gth19 16S ribosomal RNA gene, partial sequence; 16S-23S internal transcribed spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	EU723643	621	ENA	1413215 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Geobacillus thermodenitrificans subsp. thermodenitrificans DSM 465	GCA_000496575	contig	GenBank	1413215 tax ID	*
[Ref.: #66792]	Geobacillus thermodenitrificans DSM 465	1413215.3	wgs	PATRIC	1413215 tax ID	*
[Ref.: #66792]	Geobacillus thermodenitrificans DSM 465	2554235472	draft	JGI IMG/M	1413215 tax ID	*

[Ref.: #118]	GC-content	48.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #118]	GC-content	52.0 mol%

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Geobacillus thermodenitrificans DSM 465 PATRIC: 1413215.3
[Ref.: #69480]	motile	yes	89.443	no
[Ref.: #69480]	gram-positive	yes	90.663	no
[Ref.: #69480]	anaerobic	no	98.179	no
[Ref.: #69480]	halophile	no	85.803	no
[Ref.: #69480]	spore-forming	yes	95.975	no
[Ref.: #69480]	thermophile	yes	99.999	no
[Ref.: #69480]	glucose-util	yes	90.725	no
[Ref.: #69480]	aerobic	yes	90.819	no
[Ref.: #69480]	flagellated	yes	82.935	no
[Ref.: #69480]	glucose-ferment	no	92.853	no

Availability in culture collections External links

[Ref.: #118]

Culture collection no.

DSM 465, ATCC 29492, BGSC 94A1, LMG 17532

[Ref.: #71074]

SI-ID 14062

Literature:

Topic	Title	Authors	Journal	DOI	Year
Genetics	Genome Sequence of a Thermophilic Bacillus, Geobacillus thermodenitrificans DSM465.	Yao N, Ren Y, Wang W	Genome Announc	10.1128/genomeA.01046-13	2013	*
Enzymology	Green polymers from Geobacillus thermodenitrificans DSM465--candidates for molecularly imprinted materials.	Yoshikawa M, Kawamura K, Ejima A, Aoki T, Sakurai S, Hayashi K, Watanabe K	Macromol Biosci	10.1002/mabi.200500187	2006	*
Phylogeny	Isolation, characterization, and identification of Geobacillus thermodenitrificans HRO10, an alpha-amylase and alpha-glucosidase producing thermophile.	Ezeji TC, Wolf A, Bahl H	Can J Microbiol	10.1139/w05-054	2005	*
Phylogeny	A thermophilic Bacillus isolated from an Eolian shallow hydrothermal vent, able to produce exopolysaccharides.	Nicolaus B, Panico A, Manca MC, Lama L, Gambacorta A, Maugeri T, Gugliandolo C, Caccamo D	Syst Appl Microbiol	10.1016/S0723-2020(00)80074-0	2000	*
Phylogeny	Bacillus thermodenitrificans sp. nov., nom. rev.	Manachini PL, Mora D, Nicastro G, Parini C, Stackebrandt E, Pukall R, Fortina MG	Int J Syst Evol Microbiol	10.1099/00207713-50-3-1331	2000	*

References

#118	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 465

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#71074

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID14062.1 )

* These data were automatically processed and therefore are not curated

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Geobacillus thermodenitrificans DSM 465

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