Name: Sphingomonas aerolata NW12
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14233

Type strain:

Species: Sphingomonas aerolata

Strain Designation: NW12

Culture col. no.: CCUG 48435, DSM 14746, LMG 21376, CIP 108369

Strain history: CIP <- 2004, P. Kämpfer, Giessen Univ., Giessen, Germany <- O. Kim: strain NW12

NCBI tax ID(s): 185951 (species)

SI-ID 86183

LMG 21376, DSM 14746, CCUG 48435, CIP 108369

Other strains from Sphingomonas aerolata

S. aerolata CCUG 48002
S. aerolata S61/4 orange, DSM 110888

Section

Sphingomonas aerolata NW12 is an aerobe, psychrophilic, Gram-negative bacterium that was isolated from indoor air of the Sainsburry Center for Visal Art.

Gram-negative
aerobe
psychrophilic
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Sphingomonadales*
	Family	*Sphingomonadaceae*
	Genus	*Sphingomonas*
	Species	**Sphingomonas aerolata**
	Full Scientific Name (LPSN)	Sphingomonas aerolata Busse et al. 2003

Information on morphological and physiological properties Morphology

[Ref.: #116071]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.835

[Ref.: #116071]	Cell shape	rod-shaped

[Ref.: #116071]	Motility	yes

Information on culture and growth conditions Culture and growth conditions

[Ref.: #5441]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #5441]

Culture medium growth

[Ref.: #5441]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5441]

Culture medium composition

expand / minimize

[Ref.: #5441]

Culture medium

NUTRIENT AGAR (DSMZ Medium 1)

[Ref.: #5441]

Culture medium growth

[Ref.: #5441]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5441]

Culture medium composition

expand / minimize

[Ref.: #5441]

Culture medium

YEAST-PEPTONE-SUCCINATE MEDIUM (DSMZ Medium 988)

[Ref.: #5441]

Culture medium growth

[Ref.: #5441]

Culture medium link

Medium recipe at MediaDive

[Ref.: #5441]

Culture medium composition

expand / minimize

[Ref.: #37431]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37431]

Culture medium growth

[Ref.: #37431]

Culture medium composition

expand / minimize

[Ref.: #116071]

Culture medium

CIP Medium 72

[Ref.: #116071]

Culture medium growth

[Ref.: #116071]

Culture medium link

Medium recipe provided by DSMZ

[Ref.: #116071]

Culture medium

CIP Medium 566

[Ref.: #116071]

Culture medium growth

[Ref.: #116071]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #5441]		growth	22 °C
[Ref.: #37431]		growth	22 °C
[Ref.: #58157]		growth	22 °C
[Ref.: #116071]		growth	10-25 °C
[Ref.: #116071]	no	growth	5 °C
[Ref.: #116071]	no	growth	30 °C
[Ref.: #116071]	no	growth	37 °C
[Ref.: #116071]	no	growth	41 °C

[Ref.: #5441]	Temperature range	psychrophilic
[Ref.: #37431]	Temperature range	psychrophilic
[Ref.: #58157]	Temperature range	psychrophilic
[Ref.: #116071]	Temperature range	psychrophilic
[Ref.: #116071]	Temperature range	mesophilic
[Ref.: #116071]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #58157]

Oxygen tolerance

aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %97
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.985

	Salt	Tested relation		Salt conc.
[Ref.: #116071]	NaCl		growth	0	%
[Ref.: #116071]	NaCl	no	growth	2	%
[Ref.: #116071]	NaCl	no	growth	4	%
[Ref.: #116071]	NaCl	no	growth	6	%
[Ref.: #116071]	NaCl	no	growth	8	%
[Ref.: #116071]	NaCl	no	growth	10	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #68369]	adipate ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	arginine ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	D-glucose ChEBI	-	fermentation	* from API 20NE
[Ref.: #68369]	D-mannitol ChEBI	-	assimilation	* from API 20NE
[Ref.: #68369]	D-mannose ChEBI	+	assimilation	* from API 20NE
[Ref.: #116071]	esculin ChEBI	+	hydrolysis
[Ref.: #68369]	esculin ChEBI	+	hydrolysis	* from API 20NE
[Ref.: #68369]	gelatin ChEBI	-	hydrolysis	* from API 20NE
[Ref.: #68369]	L-arabinose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	malate ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	maltose ChEBI	+	assimilation	* from API 20NE
[Ref.: #68369]	N-acetylglucosamine ChEBI	+	assimilation	* from API 20NE
[Ref.: #116071]	nitrate ChEBI	-	reduction
[Ref.: #68369]	nitrate ChEBI	-	reduction	* from API 20NE
[Ref.: #116071]	nitrite ChEBI	-	reduction
[Ref.: #68369]	tryptophan ChEBI	-	energy source	* from API 20NE
[Ref.: #68369]	urea ChEBI	-	hydrolysis	* from API 20NE
	Only first 10 entries are displayed. Click here to see all.

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #116071]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	no	yes

	Metabolite production	Metabolite	Production
[Ref.: #116071]		indole ChEBI	no
[Ref.: #68369]		indole ChEBI	no	* from API 20NE

	Physiological tests	Metabolite	Indole test
[Ref.: #68369]		indole ChEBI	-	* from API 20NE

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #116071]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #116071]	amylase	-
[Ref.: #68369]	arginine dihydrolase	-	3.5.3.6	* from API 20NE
[Ref.: #116071]	beta-galactosidase	+	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68369]	beta-glucosidase	+	3.2.1.21	* from API 20NE, API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #116071]	caseinase	-	3.4.21.50
[Ref.: #116071]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #68369]	cytochrome oxidase	-	1.9.3.1	* from API 20NE
[Ref.: #116071]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #116071]	gelatinase	-
[Ref.: #68369]	gelatinase	-		* from API 20NE
[Ref.: #116071]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #116071]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #116071]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #116071]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #116071]	oxidase	-
[Ref.: #116071]	protease	-
[Ref.: #68382]	trypsin	-	3.4.21.4	* from API zym
[Ref.: #116071]	tryptophan deaminase	-
[Ref.: #116071]	tween esterase	-
[Ref.: #116071]	urease	-	3.5.1.5
[Ref.: #68369]	urease	-	3.5.1.5	* from API 20NE
[Ref.: #68382]	valine arylamidase	+		* from API zym
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #58157]

Fatty acid	Percentage	ECL
C18:1 ω7c /12t/9t	38.3	17.824
C16:1 ω7c	31.7	15.819
C16:0	9.8	16
C14:0 2OH	9.4	15.205
C16:1 ω5c	2.7	15.908
C14:0	1.6	14
11 methyl 18:1 ω7c	1.6	18.081
C17:1 ω6c	1.2	16.862
C18:1 ω5c	0.9	17.919
C16:1 2OH	0.9	17.047
C17:1 ω7c	0.6	16.818
C12:0 2OH	0.5	13.178
C16:0 iso 3OH	0.4	17.145
C15:0	0.2	15
unknown 18.555	0.2	18.555

API® 20NE:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Reduction of nitratesNO3	Indole productionTRP	Fermentation of D-glucoseGLU_ Ferm	Arginine dihydrolaseADH (Arg)	Urease/urea hydrolysisURE	Esculin hydrolysis/beta-GlucosidaseESC	Gelatinase/gelatin hydrolysisGEL	beta-GalactosidasePNPG	Assimilation of D-glucoseGLU_ Assim	Assimilation of L-arabinoseARA	Assimilation of D-mannoseMNE	Assimilation of D-mannitolMAN	Assimilation of N-acetyl-glucosamineNAG	Assimilation of maltoseMAL	Assimilation of gluconateGNT	Assimilation of capric acidCAP	Assimilation adipic acidADI	Assimilation of malic acidMLT	Assimilation of citric acidCIT	Assimilation of phenyl-acetic acidPAC	Cytochrome oxidaseOX
[Ref.: #5441]	25297	-	-	-	-	-	+	-	+	+	+	+	-	+	+	+/-	+/-	-	+	+	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #116071]	11123	-	+	+	+	-	+	+	-	-	-	+	+	-	-	+	+	+	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #5441]	Sample type/isolated from	indoor air of the Sainsburry Center for Visal Art
[Ref.: #5441]	Geographic location (country and/or sea, region)	Norwich
[Ref.: #5441]	Continent	Europe

[Ref.: #58157]	Sample type/isolated from	Air
[Ref.: #58157]	Geographic location (country and/or sea, region)	Norwich,Sainsbury Centre for Vis.Arts
[Ref.: #58157]	Country	United Kingdom
[Ref.: #58157]	Country ISO 3 Code	GBR
[Ref.: #58157]	Continent	Europe

[Ref.: #116071]	Sample type/isolated from	Environment, Indoor air of Sainsburry Center for Visal Art
[Ref.: #116071]	Geographic location (country and/or sea, region)	Norwich
[Ref.: #116071]	Country	United Kingdom
[Ref.: #116071]	Country ISO 3 Code	GBR
[Ref.: #116071]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	-
#Environmental	#Air	#Indoor Air

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AJ429240 (>99% sequence identity) for Sphingomonas from Microbeatlas

Aquatic Samples	7018
Soil Samples	6757
Animal Samples	12267
Plant Samples	9299
Total Samples	35341

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #5441]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #116071]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Sphingomonas aerolata partial 16S rRNA gene, strain NW12	AJ429240	1472	ENA	185951 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Sphingomonas aerolata NW12	GCA_003046295	scaffold	GenBank	185951 tax ID	*
[Ref.: #66792]	Sphingomonas aerolata NW12	2740892601	draft	JGI IMG/M	185951 tax ID	*

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Sphingomonas aerolata NW12 NCBI: GCA_003046295.1
[Ref.: #69481]	spore-forming	no	97	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Sphingomonas aerolata NW12 NCBI: GCA_003046295.1
[Ref.: #69480]	flagellated	no	89.805	no
[Ref.: #69480]	gram-positive	no	97.129	no
[Ref.: #69480]	anaerobic	no	99.192	yes
[Ref.: #69480]	halophile	no	92.481	no
[Ref.: #69480]	spore-forming	no	94.751	no
[Ref.: #69480]	thermophile	no	99.074	no
[Ref.: #69480]	glucose-util	yes	89.698	no
[Ref.: #69480]	aerobic	yes	92.413	yes
[Ref.: #69480]	motile	yes	67.014	no
[Ref.: #69480]	glucose-ferment	no	87.828	no

Availability in culture collections External links

[Ref.: #5441]

Culture collection no.

CCUG 48435, DSM 14746, LMG 21376, CIP 108369

[Ref.: #83392]

SI-ID 86183

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sphingomonas aurantiaca sp. nov., Sphingomonas aerolata sp. nov. and Sphingomonas faeni sp. nov., air- and dustborne and Antarctic, orange-pigmented, psychrotolerant bacteria, and emended description of the genus Sphingomonas.	Busse HJ, Denner EBM, Buczolits S, Salkinoja-Salonen M, Bennasar A, Kampfer P	Int J Syst Evol Microbiol	10.1099/ijs.0.02461-0	2003	*

References

#5441

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 14746

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37431 ; Curators of the CIP;
#58157 Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 48435

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#68369

Automatically annotated from API 20NE .

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83392

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID86183.1 )

#116071 Collection of Institut Pasteur ; Curators of the CIP; CIP 108369
* These data were automatically processed and therefore are not curated

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