Clostridium botulinum (CCUG 7970, NCTC 8264)

Name: Clostridium botulinum (CCUG 7970, NCTC 8264)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 141867

Type strain

Culture col. no. CCUG 7970 NCTC 8264

NCBI tax ID(s) 1491

Special Collections CCUG

Links

Clostridium botulinum CCUG 7970 is an anaerobe, mesophilic prokaryote of the family Clostridiaceae.

anaerobe mesophilic genome sequence

Name and taxonomic classification

SI-ID 52860

CCUG 7970,NCTC 8264

@ref 20215

Last LPSN update 2026-02-09 11:34:03

Domain Bacteria

Phylum Bacillota

Class Clostridia

Order Eubacteriales

Family Clostridiaceae

Genus Clostridium

Species Clostridium botulinum

Full scientific name Clostridium botulinum (van Ermengem 1896) Bergey et al. 1923 (Approved Lists 1980)

Synonyms (1)

"Bacillus botulinus"

BacDive ID	Other strains from **Clostridium botulinum** (14)	Type strain
166627	C. botulinum CIP 104310, ATCC 25763 (type strain)
141845	C. botulinum CCUG 7735
141865	C. botulinum CCUG 7968, ATCC 19397, NCTC 7272
141866	C. botulinum CCUG 7969, NCTC 7273
141868	C. botulinum CCUG 7971, NCTC 8265
141869	C. botulinum CCUG 7974, NCTC 8266
142345	C. botulinum CCUG 11910
142708	C. botulinum CCUG 14228
144075	C. botulinum CCUG 22775
146789	C. botulinum CCUG 32121
146790	C. botulinum CCUG 32122
146791	C. botulinum CCUG 32123
149343	C. botulinum CCUG 37546
150472	C. botulinum CCUG 41389

Morphology

Colony morphology

45053

Incubation period1 day

Culture and growth conditions

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
45053	positive	growth	37	mesophilic

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
45053	anaerobe
125438	anaerobe	90.826
125439	anaerobe	97.6

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68380	29016 ChEBI	arginine	-	hydrolysis	from API rID32A
68380	16024 ChEBI	D-mannose	-	fermentation	from API rID32A
68380	29985 ChEBI	L-glutamate	-	degradation	from API rID32A
68380	17632 ChEBI	nitrate	-	reduction	from API rID32A
68380	16634 ChEBI	raffinose	-	fermentation	from API rID32A
68380	27897 ChEBI	tryptophan	+	energy source	from API rID32A
68380	16199 ChEBI	urea	-	hydrolysis	from API rID32A

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68380	35581 ChEBI	indole		from API rID32A

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68380	35581 ChEBI	indole	+	from API rID32A

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
68380	alanine arylamidase	-	3.4.11.2	from API rID32A
68382	alkaline phosphatase	-	3.1.3.1	from API zym
68380	alkaline phosphatase	-	3.1.3.1	from API rID32A
68380	alpha-arabinosidase	-	3.2.1.55	from API rID32A
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68380	alpha-fucosidase	-	3.2.1.51	from API rID32A
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68380	alpha-galactosidase	-	3.2.1.22	from API rID32A
68382	alpha-glucosidase	-	3.2.1.20	from API zym
68380	alpha-glucosidase	-	3.2.1.20	from API rID32A
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68380	arginine dihydrolase	-	3.5.3.6	from API rID32A
68382	beta-galactosidase	-	3.2.1.23	from API zym
68380	beta-galactosidase	-	3.2.1.23	from API rID32A
68380	beta-Galactosidase 6-phosphate	-		from API rID32A
68382	beta-glucosidase	-	3.2.1.21	from API zym
68380	beta-glucosidase	-	3.2.1.21	from API rID32A
68382	beta-glucuronidase	-	3.2.1.31	from API zym
68380	beta-glucuronidase	-	3.2.1.31	from API rID32A
68382	cystine arylamidase	-	3.4.11.3	from API zym
68382	esterase (C 4)	-		from API zym
68382	esterase lipase (C 8)	-		from API zym
68380	glutamate decarboxylase	-	4.1.1.15	from API rID32A
68380	glutamyl-glutamate arylamidase	-		from API rID32A
68380	glycin arylamidase	-		from API rID32A
68380	histidine arylamidase	-		from API rID32A
68380	L-arginine arylamidase	-		from API rID32A
68382	leucine arylamidase	-	3.4.11.1	from API zym
68380	leucine arylamidase	-	3.4.11.1	from API rID32A
68380	leucyl glycin arylamidase	-	3.4.11.1	from API rID32A
68382	lipase (C 14)	-		from API zym
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68380	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API rID32A
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
68380	phenylalanine arylamidase	-		from API rID32A
68380	proline-arylamidase	-	3.4.11.5	from API rID32A
68380	pyrrolidonyl arylamidase	-	3.4.19.3	from API rID32A
68380	serine arylamidase	-		from API rID32A
68382	trypsin	+	3.4.21.4	from API zym
68380	tryptophan deaminase	+	4.1.99.1	from API rID32A
68380	tyrosine arylamidase	-		from API rID32A
68380	urease	-	3.5.1.5	from API rID32A
68382	valine arylamidase	-		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
45053	-	-	-	-	-	-	-	-	+	-	+	-	-	-	-	-	-	-	-	-

API rID32A

@ref	URE	ADH (Arg)	alpha GAL	beta GAL	beta-Galactosidase 6-phosphatebeta GP	alpha GLU	beta GLU	alpha ARA	beta GUR	beta-N-Acetyl-beta-glucosaminidasebeta NAG	MNE	RAF	GDC	alpha FUC	Reduction of nitrateNIT	IND	PAL	L-arginine arylamidaseArgA	ProA	LGA	Phenylalanine arylamidasePheA	Leucine arylamidaseLeuA	PyrA	Tyrosine arylamidaseTyrA	Alanine arylamidaseAlaA	Glycin arylamidaseGlyA	Histidine arylamidaseHisA	Glutamyl-glutamate arylamidaseGGA	Serine arylamidaseSerA
45053	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-

Isolation, sampling and environmental information

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	NCBI tax ID	Score
66792	ASM1759015v1 assembly for Clostridium haemolyticum CCUG 7970	contig	GCA_017590155	84025.6	84025	40.34

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Clostridium haemolyticum CCUG 7970
NCBI: GCA_017590155

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	86.00	no
125439	motility	BacteriaNetⓘ	yes	83.50	no
125439	gram_stain	BacteriaNetⓘ	positive	79.20	no
125439	oxygen_tolerance	BacteriaNetⓘ	anaerobe	97.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Clostridium haemolyticum strain CCUG 7970
PATRIC: 84025.6

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	66.09	no
125438	anaerobic	anaerobicⓘ	yes	90.83	yes
125438	aerobic	aerobicⓘ	no	98.98	no
125438	spore-forming	spore-formingⓘ	yes	64.96	no
125438	thermophilic	thermophileⓘ	no	89.23	yes
125438	flagellated	motile2+ⓘ	yes	61.81	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Invasive slug populations (Arion vulgaris) as potential vectors for Clostridium botulinum.	Gismervik K, Bruheim T, Rorvik LM, Haukeland S, Skaar I.	Acta Vet Scand	10.1186/s13028-014-0065-z	2014
	Pentaplexed quantitative real-time PCR assay for the simultaneous detection and quantification of botulinum neurotoxin-producing clostridia in food and clinical samples.	Kirchner S, Kramer KM, Schulze M, Pauly D, Jacob D, Gessler F, Nitsche A, Dorner BG, Dorner MB.	Appl Environ Microbiol	10.1128/aem.02490-09	2010
Enzymology	Sensitive detection of botulinum neurotoxin types C and D with an immunoaffinity chromatographic column test.	Gessler F, Hampe K, Bohnel H.	Appl Environ Microbiol	10.1128/aem.71.12.7897-7903.2005	2005
	Detection of the genes encoding botulinum neurotoxin types A to E by the polymerase chain reaction.	Szabo EA, Pemberton JM, Desmarchelier PM.	Appl Environ Microbiol	10.1128/aem.59.9.3011-3020.1993	1993
	Cloning of a DNA sequence unique to Clostridium botulinum group I by selective hybridization.	McKinney MW, Levett PN, Haylock RW.	J Clin Microbiol	10.1128/jcm.31.7.1845-1849.1993	1993
	Application of PCR to a clinical and environmental investigation of a case of equine botulism.	Szabo EA, Pemberton JM, Gibson AM, Thomas RJ, Pascoe RR, Desmarchelier PM.	J Clin Microbiol	10.1128/jcm.32.8.1986-1991.1994	1994
Enzymology	Specific detection of Clostridium botulinum type B by using the polymerase chain reaction.	Szabo EA, Pemberton JM, Desmarchelier PM.	Appl Environ Microbiol	10.1128/aem.58.1.418-420.1992	1992
Enzymology	Isolation of Clostridium botulinum type G from Swiss soil specimens by using sequential steps in an identification scheme.	Sonnabend WF, Sonnabend UP, Krech T.	Appl Environ Microbiol	10.1128/aem.53.8.1880-1884.1987	1987

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#45053	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 7970
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#68380	Automatically annotated from API rID32A .
#68382	Automatically annotated from API zym .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Clostridium botulinum (CCUG 7970, NCTC 8264)

Name and taxonomic classification

Morphology

Colony morphology

Culture and growth conditions

Culture temp

Physiology and metabolism

Oxygen tolerance

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API rID32A

Isolation, sampling and environmental information

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_017590155

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

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