Novosphingobium lentum (DSM 13663, CCUG 45847, CIP 108698)

Name: Novosphingobium lentum (DSM 13663, CCUG 45847, CIP 108698)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 14153

Type strain

Strain Designation MT1

Culture col. no. DSM 13663 CCUG 45847 CIP 108698 MT1

NCBI tax ID(s) 145287

Special Collections DSMZ CCUG CIP Literature strains

History <- M. Tiirola; MT1 CIP <- 2005, CCUG <- 2001, M.A. Tiirola, Jyväskylä, Finland: strain MT1

Links

Novosphingobium lentum MT1 is an aerobe, Gram-negative, rod-shaped bacterium that was isolated from bioreactor prifying polychlorophenol contaminated graoundwater.

Gram-negative rod-shaped aerobe 16S sequence Bacteria

Name and taxonomic classification

SI-ID 129068

NBRC 107847,DSM 13663,CCUG 45847,CIP 108698

@ref 20215

Last LPSN update 2026-02-09 11:21:20

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Sphingomonadales

Family Erythrobacteraceae

Genus Novosphingobium

Species Novosphingobium lentum

Full scientific name Novosphingobium lentum Tiirola et al. 2005

BacDive ID	Other strains from **Novosphingobium lentum** (4)	Type strain
14154	N. lentum MT1 Chl-, MT1 Chl^-, DSM 13664
152123	N. lentum CCUG 45849
152124	N. lentum CCUG 45850
152125	N. lentum CCUG 45851

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
31354	negative	1.05 µm	0.4 µm	rod-shaped
120582	negative			rod-shaped

Pigmentation

31354

Productionyes

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
4945	REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c)	Medium recipe at MediaDive	Name: REACTIVATION WITH LIQUID MEDIUM 830 (DSMZ Medium 830c) Composition: Agar 15.0 g/l Yeast extract 0.5 g/l Proteose peptone 0.5 g/l Casamino acids 0.5 g/l Glucose 0.5 g/l Starch 0.5 g/l K2HPO4 0.3 g/l Na-pyruvate 0.3 g/l MgSO4 x 7 H2O 0.05 g/l Distilled water
33263	MEDIUM 566- Reasoner's 2A agar for Flavobacterium micromati		Distilled water make up to (1000.000 ml);R2A agar (18.200 g)
120582	CIP Medium 566	Medium recipe at CIP

Culture temp

@ref	Growth	Type	Temperature (°C)
4945	positive	growth	20
31354	positive	growth	04-30
31354	positive	optimum	23
33263	positive	growth	22
56947	positive	growth	20-26
120582	positive	growth	22-30

Physiology and metabolism

Oxygen tolerance

31354

Oxygen toleranceaerobe

Spore formation

31354

Spore formationno

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
31354	16449 ChEBI	alanine	+	carbon source
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
31354	17632 ChEBI	nitrate	+	reduction
120582	17632 ChEBI	nitrate	+	reduction
120582	17632 ChEBI	nitrate	-	respiration
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
120582	16301 ChEBI	nitrite	-	reduction
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Metabolite production

@ref	Chebi-ID	Metabolite	Production
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
68382	acid phosphatase	+	3.1.3.2	from API zym
120582	alcohol dehydrogenase	-	1.1.1.1
68382	alkaline phosphatase	+	3.1.3.1	from API zym
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
68382	alpha-fucosidase	-	3.2.1.51	from API zym
68382	alpha-galactosidase	-	3.2.1.22	from API zym
68382	alpha-glucosidase	+	3.2.1.20	from API zym
68382	alpha-mannosidase	-	3.2.1.24	from API zym
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
68382	beta-galactosidase	-	3.2.1.23	from API zym
68382	beta-glucosidase	+	3.2.1.21	from API zym
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
68382	beta-glucuronidase	-	3.2.1.31	from API zym
31354	catalase	+	1.11.1.6
120582	catalase	+	1.11.1.6
68382	cystine arylamidase	-	3.4.11.3	from API zym
68369	cytochrome oxidase	+	1.9.3.1	from API 20NE
68382	esterase (C 4)	+		from API zym
68382	esterase lipase (C 8)	+		from API zym
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
68382	lipase (C 14)	-		from API zym
120582	lysine decarboxylase	-	4.1.1.18
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
68382	naphthol-AS-BI-phosphohydrolase	+		from API zym
120582	ornithine decarboxylase	-	4.1.1.17
120582	oxidase	+
68382	trypsin	+	3.4.21.4	from API zym
68369	urease	-	3.5.1.5	from API 20NE
68382	valine arylamidase	+		from API zym

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
120582	-	+	+	+	-	+	+	-	+	-	+	+	-	-	-	+	+	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
4945	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+
4945	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Engineered	#Bioreactor	-
#Engineered	#Contamination	-
#Environmental	#Aquatic	#Groundwater

Isolation

@ref	Sample type	Sampling date	Geographic location	Country	Country ISO 3 Code	Continent	Isolation date
4945	bioreactor prifying polychlorophenol contaminated graoundwater		Kärkölä	Finland	FIN	Europe
56947	Sludge fed to fluidized-bed bioreactor	1999	Kärkölä	Finland	FIN	Europe
120582	Environment, Sludge fed		Kärkölä	Finland	FIN	Europe	1999

Taxonmaps

69479

Global distribution of 16S sequence AJ303009 (>99% sequence identity) for Novosphingobium lentum subclade from Microbeatlas

Aquatic Samples	573
Soil Samples	288
Animal Samples	176
Plant Samples	63
Total Samples	1100

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
4945	1	Risk group (German classification) According to TRBA 466
120582	1	Risk group (French classification) According to TRBA 466

Sequence information

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
4945	Sphingomonas sp. MT1 partial 16S rRNA gene	AJ303009	1440		1219033

GC content

4945

GC-content (mol%)66

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Novosphingobium lentum sp. nov., a psychrotolerant bacterium from a polychlorophenol bioremediation process.	Tiirola MA, Busse HJ, Kampfer P, Mannisto MK	Int J Syst Evol Microbiol	10.1099/ijs.0.63386-0	2005

References

#4945	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 13663
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#27667	IJSEM 583 2005 ( DOI 10.1099/ijs.0.63386-0 , PubMed 15774628 )
#31354	Barberan A, Caceres Velazquez H, Jones S, Fierer N.: Hiding in Plain Sight: Mining Bacterial Species Records for Phenotypic Trait Information. mSphere 2: 2017 ( DOI 10.1128/mSphere.00237-17 , PubMed 28776041 ) - originally annotated from #27667
#33263	; Curators of the CIP;
#56947	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 45847
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#120582	Collection of Institut Pasteur ; Curators of the CIP; CIP 108698
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Novosphingobium lentum (DSM 13663, CCUG 45847, CIP 108698)

Name and taxonomic classification

Morphology

Cell morphology

Pigmentation

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Oxygen tolerance

Spore formation

Metabolite utilization

Metabolite production

Metabolite tests

Enzymes

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

Help

Social Media