Francisella tularensis (CCUG 2112, ATCC 6223)

Name: Francisella tularensis (CCUG 2112, ATCC 6223)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 141470

Type strain

Culture col. no. CCUG 2112 ATCC 6223

NCBI tax ID(s) 263 119856

Special Collections CCUG

Links

Francisella tularensis CCUG 2112 is a prokaryote of the family Francisellaceae.

16S sequence

Name and taxonomic classification

SI-ID 54022

CCUG 2112,ATCC 6223

@ref 20215

Last LPSN update 2025-12-16 10:03:52

Domain Pseudomonadati

Phylum Pseudomonadota

Class Gammaproteobacteria

Order Beggiatoales

Family Francisellaceae

Genus Francisella

Species Francisella tularensis

Full scientific name Francisella tularensis (McCoy and Chapin 1912) Dorofeev 1947 (Approved Lists 1980)

Synonyms (1)

"Bacterium tularense"

BacDive ID	Other strains from **Francisella tularensis** (9)	Type strain
147276	F. tularensis CCUG 33270
147337	F. tularensis CCUG 33391
152933	F. tularensis CCUG 48060
152962	F. tularensis CCUG 48139
153935	F. tularensis CCUG 51689
154382	F. tularensis CCUG 53311
155291	F. tularensis CCUG 57222
155930	F. tularensis CCUG 59671, FSC 855
155931	F. tularensis CCUG 59672, FSC 856

Morphology

Cell morphology

@ref	Gram stain	Confidence
125439	negative	99.4

Culture and growth conditions

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
125439	obligate aerobe	94

Spore formation

@ref	Spore formation	Confidence
125439		99.3

Isolation, sampling and environmental information

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	IMG accession	NCBI tax ID	Score
66792	Francisella tularensis ATCC 6223	complete	2505119032	491120	80.38

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
124043	F.tularensis (ATCC 6223) 16S rRNA (partial)	Z22912	172		263
124043	F.tularensis (ATCC 6223) 16S rRNA (partial)	Z22913	249		263

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Francisella tularensis ATCC 6223
IMG: 2505119032

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	99.30	no
125439	motility	BacteriaNetⓘ	no	62.70	no
125439	gram_stain	BacteriaNetⓘ	negative	99.40	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	94.00	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Comprehensive Laboratory Evaluation of a Specific Lateral Flow Assay for the Presumptive Identification of Francisella tularensis in Suspicious White Powders and Aerosol Samples.	Pillai SP, DePalma L, Prentice KW, Ramage JG, Chapman C, Sarwar J, Parameswaran N, Petersen J, Yockey B, Young J, Singh A, Pillai CA, Manickam G, Thirunavkkarasu N, Avila JR, Sharma S, Morse SA, Venkateswaran K, Anderson K, Hodge DR.	Health Secur	10.1089/hs.2019.0151	2020
	Detection of Francisellaceae and the differentiation of main European F. tularensis ssp. holarctica strains (Clades) by new designed qPCR assays.	Koppen K, Rydzewski K, Zajac J, Al-Senwi M, Evcimen S, Schulze D, Jacob D, Heuner K.	BMC Microbiol	10.1186/s12866-025-03751-9	2025
	Identification of Francisella tularensis Subspecies in a Clinical Setting Using MALDI-TOF MS: An In-House Francisella Library and Biomarkers.	de Vries MC, Hoeve-Bakker BJA, van den Beld MJC, Hendriks ACA, Harpal ASD, Noomen RCEA, Reubsaet FAG.	Microorganisms	10.3390/microorganisms11040905	2023
	Monitoring Campaign over an Edible Dormouse Population (Glis glis; Rodentia: Gliridae) in Sicily: First Report of Mesocestodiasis.	Schiro G, Pieri D, Lo Valvo M, Gradoni L, Caccio SM, Severini F, Marucci G, Galuppo L, Cumbo V, Puleio R, Loria GR.	Animals (Basel)	10.3390/ani11040934	2021
	Differentiation of Francisella tularensis Subspecies and Subtypes.	Larson MA, Sayood K, Bartling AM, Meyer JR, Starr C, Baldwin J, Dempsey MP.	J Clin Microbiol	10.1128/jcm.01495-19	2020
Genetics	Phylogeography and Genetic Diversity of Francisella tularensis subsp. holarctica in France (1947-2018).	Kevin M, Girault G, Caspar Y, Cherfa MA, Mendy C, Tomaso H, Gavier-Widen D, Escudero R, Maurin M, Durand B, Ponsart C, Madani N.	Front Microbiol	10.3389/fmicb.2020.00287	2020
Pathogenicity	In Vitro Antibiotic Susceptibilities of Francisella tularensis Determined by Broth Microdilution following CLSI Methods.	Heine HS, Miller L, Halasohoris S, Purcell BK.	Antimicrob Agents Chemother	10.1128/aac.00612-17	2017
Cultivation	A novel broth medium for enhanced growth of Francisella tularensis.	Morris BJ, Buse HY, Adcock NJ, Rice EW.	Lett Appl Microbiol	10.1111/lam.12725	2017
Pathogenicity	Acid resistance in Francisella tularensis.	Adcock NJ, Morris BJ, Rice EW.	Microbiologyopen	10.1002/mbo3.151	2014
	Comparison among the Quantification of Bacterial Pathogens by qPCR, dPCR, and Cultural Methods.	Ricchi M, Bertasio C, Boniotti MB, Vicari N, Russo S, Tilola M, Bellotti MA, Bertasi B.	Front Microbiol	10.3389/fmicb.2017.01174	2017
	Russian isolates enlarge the known geographic diversity of Francisella tularensis subsp. mediasiatica.	Timofeev V, Bakhteeva I, Titareva G, Kopylov P, Christiany D, Mokrievich A, Dyatlov I, Vergnaud G.	PLoS One	10.1371/journal.pone.0183714	2017
	Review of Requests to Exclude Attenuated Strains of Select Agents and Modified Select Toxins, Division of Select Agents and Toxins, Centers for Disease Control and Prevention, 2003-2017.	Smith J, Gangadharan D, Hemphill M, Edwin S.	Health Secur	10.1089/hs.2018.0070	2018
	Francisella tularensis: No Evidence for Transovarial Transmission in the Tularemia Tick Vectors Dermacentor reticulatus and Ixodes ricinus.	Genchi M, Prati P, Vicari N, Manfredini A, Sacchi L, Clementi E, Bandi C, Epis S, Fabbi M.	PLoS One	10.1371/journal.pone.0133593	2015
Phylogeny	Multiple Francisella tularensis subspecies and clades, tularemia outbreak, Utah.	Petersen JM, Carlson JK, Dietrich G, Eisen RJ, Coombs J, Janusz AM, Summers J, Beard CB, Mead PS.	Emerg Infect Dis	10.3201/eid1412.080482	2008
	Canonical insertion-deletion markers for rapid DNA typing of Francisella tularensis.	Larsson P, Svensson K, Karlsson L, Guala D, Granberg M, Forsman M, Johanssont A.	Emerg Infect Dis	10.3201/eid1311.070603	2007
	Comparison and analysis of biological agent category lists based on biosafety and biodefense.	Tian D, Zheng T.	PLoS One	10.1371/journal.pone.0101163	2014
Genetics	Chromosome rearrangement and diversification of Francisella tularensis revealed by the type B (OSU18) genome sequence.	Petrosino JF, Xiang Q, Karpathy SE, Jiang H, Yerrapragada S, Liu Y, Gioia J, Hemphill L, Gonzalez A, Raghavan TM, Uzman A, Fox GE, Highlander S, Reichard M, Morton RJ, Clinkenbeard KD, Weinstock GM.	J Bacteriol	10.1128/jb.00506-06	2006
Phylogeny	Identification of Francisella tularensis by whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry: fast, reliable, robust, and cost-effective differentiation on species and subspecies levels.	Seibold E, Maier T, Kostrzewa M, Zeman E, Splettstoesser W.	J Clin Microbiol	10.1128/jcm.01953-09	2010
Phylogeny	Phylogeography of Francisella tularensis: global expansion of a highly fit clone.	Vogler AJ, Birdsell D, Price LB, Bowers JR, Beckstrom-Sternberg SM, Auerbach RK, Beckstrom-Sternberg JS, Johansson A, Clare A, Buchhagen JL, Petersen JM, Pearson T, Vaissaire J, Dempsey MP, Foxall P, Engelthaler DM, Wagner DM, Keim P.	J Bacteriol	10.1128/jb.01786-08	2009
	Evaluation of an immunochromatographic test for rapid and reliable serodiagnosis of human tularemia and detection of Francisella tularensis-specific antibodies in sera from different mammalian species.	Splettstoesser W, Guglielmo-Viret V, Seibold E, Thullier P.	J Clin Microbiol	10.1128/jcm.01475-09	2010
	Population structure of Francisella tularensis.	Nubel U, Reissbrodt R, Weller A, Grunow R, Porsch-Ozcurumez M, Tomaso H, Hofer E, Splettstoesser W, Finke EJ, Tschape H, Witte W.	J Bacteriol	10.1128/jb.01662-05	2006
Enzymology	Francisella tularensis in the United States.	Farlow J, Wagner DM, Dukerich M, Stanley M, Chu M, Kubota K, Petersen J, Keim P.	Emerg Infect Dis	10.3201/eid1112.050728	2005
Enzymology	Development of a bead-based Luminex assay using lipopolysaccharide specific monoclonal antibodies to detect biological threats from Brucella species.	Silbereisen A, Tamborrini M, Wittwer M, Schurch N, Pluschke G.	BMC Microbiol	10.1186/s12866-015-0534-1	2015
Phylogeny	Genome-wide DNA microarray analysis of Francisella tularensis strains demonstrates extensive genetic conservation within the species but identifies regions that are unique to the highly virulent F. tularensis subsp. tularensis.	Broekhuijsen M, Larsson P, Johansson A, Bystrom M, Eriksson U, Larsson E, Prior RG, Sjostedt A, Titball RW, Forsman M.	J Clin Microbiol	10.1128/jcm.41.7.2924-2931.2003	2003
Genetics	Complete genomic characterization of a pathogenic A.II strain of Francisella tularensis subspecies tularensis.	Beckstrom-Sternberg SM, Auerbach RK, Godbole S, Pearson JV, Beckstrom-Sternberg JS, Deng Z, Munk C, Kubota K, Zhou Y, Bruce D, Noronha J, Scheuermann RH, Wang A, Wei X, Wang J, Hao J, Wagner DM, Brettin TS, Brown N, Gilna P, Keim PS.	PLoS One	10.1371/journal.pone.0000947	2007
	A Survey of Antimicrobial Resistance Determinants in Category A Select Agents, Exempt Strains, and Near-Neighbor Species.	Taitt CR, Leski TA, Chen A, Berk KL, Dorsey RW, Gregory MJ, Sozhamannan S, Frey KG, Dutt DL, Vora GJ.	Int J Mol Sci	10.3390/ijms21051669	2020
	Potential source of Francisella tularensis live vaccine strain attenuation determined by genome comparison.	Rohmer L, Brittnacher M, Svensson K, Buckley D, Haugen E, Zhou Y, Chang J, Levy R, Hayden H, Forsman M, Olson M, Johansson A, Kaul R, Miller SI.	Infect Immun	10.1128/iai.01006-06	2006
Enzymology	Reliable detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis by using multiplex qPCR including internal controls for nucleic acid extraction and amplification.	Janse I, Hamidjaja RA, Bok JM, van Rotterdam BJ.	BMC Microbiol	10.1186/1471-2180-10-314	2010
	Resistance of Francisella novicida to fosmidomycin associated with mutations in the glycerol-3-phosphate transporter.	Mackie RS, McKenney ES, van Hoek ML.	Front Microbiol	10.3389/fmicb.2012.00226	2012
	Molecular method for discrimination between Francisella tularensis and Francisella-like endosymbionts.	Escudero R, Toledo A, Gil H, Kovacsova K, Rodriguez-Vargas M, Jado I, Garcia-Amil C, Lobo B, Bhide M, Anda P.	J Clin Microbiol	10.1128/jcm.00275-08	2008
Phylogeny	Paired-end sequence mapping detects extensive genomic rearrangement and translocation during divergence of Francisella tularensis subsp. tularensis and Francisella tularensis subsp. holarctica populations.	Dempsey MP, Nietfeldt J, Ravel J, Hinrichs S, Crawford R, Benson AK.	J Bacteriol	10.1128/jb.00437-06	2006
Phylogeny	Whole genome single nucleotide polymorphism based phylogeny of Francisella tularensis and its application to the development of a strain typing assay.	Pandya GA, Holmes MH, Petersen JM, Pradhan S, Karamycheva SA, Wolcott MJ, Molins C, Jones M, Schriefer ME, Fleischmann RD, Peterson SN.	BMC Microbiol	10.1186/1471-2180-9-213	2009
Phylogeny	Worldwide genetic relationships among Francisella tularensis isolates determined by multiple-locus variable-number tandem repeat analysis.	Johansson A, Farlow J, Larsson P, Dukerich M, Chambers E, Bystrom M, Fox J, Chu M, Forsman M, Sjostedt A, Keim P.	J Bacteriol	10.1128/jb.186.17.5808-5818.2004	2004
	Pathogen security-help or hindrance?	Morse SA.	Front Bioeng Biotechnol	10.3389/fbioe.2014.00083	2014
	Rapid focused sequencing: a multiplexed assay for simultaneous detection and strain typing of Bacillus anthracis, Francisella tularensis, and Yersinia pestis.	Turingan RS, Thomann HU, Zolotova A, Tan E, Selden RF.	PLoS One	10.1371/journal.pone.0056093	2013
Enzymology	Detection of Francisella tularensis in biological specimens using a capture enzyme-linked immunosorbent assay, an immunochromatographic handheld assay, and a PCR.	Grunow R, Splettstoesser W, McDonald S, Otterbein C, O'Brien T, Morgan C, Aldrich J, Hofer E, Finke EJ, Meyer H.	Clin Diagn Lab Immunol	10.1128/cdli.7.1.86-90.2000	2000
Genetics	Molecular evolutionary consequences of niche restriction in Francisella tularensis, a facultative intracellular pathogen.	Larsson P, Elfsmark D, Svensson K, Wikstrom P, Forsman M, Brettin T, Keim P, Johansson A.	PLoS Pathog	10.1371/journal.ppat.1000472	2009
Phylogeny	Evaluation of PCR-based methods for discrimination of Francisella species and subspecies and development of a specific PCR that distinguishes the two major subspecies of Francisella tularensis.	Johansson A, Ibrahim A, Goransson I, Eriksson U, Gurycova D, Clarridge JE, Sjostedt A.	J Clin Microbiol	10.1128/jcm.38.11.4180-4185.2000	2000
Phylogeny	Genotyping of Francisella tularensis strains by pulsed-field gel electrophoresis, amplified fragment length polymorphism fingerprinting, and 16S rRNA gene sequencing.	Garcia Del Blanco N, Dobson ME, Vela AI, De La Puente VA, Gutierrez CB, Hadfield TL, Kuhnert P, Frey J, Dominguez L, Rodriguez Ferri EF.	J Clin Microbiol	10.1128/jcm.40.8.2964-2972.2002	2002
Phylogeny	Rapid differentiation of Francisella species and subspecies by fluorescent in situ hybridization targeting the 23S rRNA.	Splettstoesser WD, Seibold E, Zeman E, Trebesius K, Podbielski A.	BMC Microbiol	10.1186/1471-2180-10-72	2010
	Comparison of enzyme-linked immunosorbent assay, Western blotting, microagglutination, indirect immunofluorescence assay, and flow cytometry for serological diagnosis of tularemia.	Porsch-Ozcurumez M, Kischel N, Priebe H, Splettstosser W, Finke EJ, Grunow R.	Clin Diagn Lab Immunol	10.1128/cdli.11.6.1008-1015.2004	2004
Metabolism	Characterization of the pathogenicity island protein PdpA and its role in the virulence of Francisella novicida.	Schmerk CL, Duplantis BN, Wang D, Burke RD, Chou AY, Elkins KL, Ludu JS, Nano FE.	Microbiology (Reading)	10.1099/mic.0.025379-0	2009
Enzymology	Comparison of hand-held test kits, immunofluorescence microscopy, enzyme-linked immunosorbent assay, and flow cytometric analysis for rapid presumptive identification of Yersinia pestis.	Tomaso H, Thullier P, Seibold E, Guglielmo V, Buckendahl A, Rahalison L, Neubauer H, Scholz HC, Splettstoesser WD.	J Clin Microbiol	10.1128/jcm.00458-07	2007
Enzymology	Development and comparison of two assay formats for parallel detection of four biothreat pathogens by using suspension microarrays.	Janse I, Bok JM, Hamidjaja RA, Hodemaekers HM, van Rotterdam BJ.	PLoS One	10.1371/journal.pone.0031958	2012
Phylogeny	Characterization and classification of strains of Francisella tularensis isolated in the central Asian focus of the Soviet Union and in Japan.	Sandstrom G, Sjostedt A, Forsman M, Pavlovich NV, Mishankin BN.	J Clin Microbiol	10.1128/jcm.30.1.172-175.1992	1992
Pathogenicity	Azithromycin effectiveness against intracellular infections of Francisella.	Ahmad S, Hunter L, Qin A, Mann BJ, van Hoek ML.	BMC Microbiol	10.1186/1471-2180-10-123	2010
	A Francisella tularensis pathogenicity island required for intramacrophage growth.	Nano FE, Zhang N, Cowley SC, Klose KE, Cheung KK, Roberts MJ, Ludu JS, Letendre GW, Meierovics AI, Stephens G, Elkins KL.	J Bacteriol	10.1128/jb.186.19.6430-6436.2004	2004
Phylogeny	Extensive allelic variation among Francisella tularensis strains in a short-sequence tandem repeat region.	Johansson A, Goransson I, Larsson P, Sjostedt A.	J Clin Microbiol	10.1128/jcm.39.9.3140-3146.2001	2001
Phylogeny	Pathoscope: species identification and strain attribution with unassembled sequencing data.	Francis OE, Bendall M, Manimaran S, Hong C, Clement NL, Castro-Nallar E, Snell Q, Schaalje GB, Clement MJ, Crandall KA, Johnson WE.	Genome Res	10.1101/gr.150151.112	2013
Phylogeny	Characterization of two unusual clinically significant Francisella strains.	Clarridge JE, Raich TJ, Sjosted A, Sandstrom G, Darouiche RO, Shawar RM, Georghiou PR, Osting C, Vo L.	J Clin Microbiol	10.1128/jcm.34.8.1995-2000.1996	1996
Enzymology	Identification of Francisella species and discrimination of type A and type B strains of F. tularensis by 16S rRNA analysis.	Forsman M, Sandstrom G, Jaurin B.	Appl Environ Microbiol	10.1128/aem.56.4.949-955.1990	1990
Pathogenicity	Antimicrobial susceptibility testing of Francisella tularensis with a modified Mueller-Hinton broth.	Baker CN, Hollis DG, Thornsberry C.	J Clin Microbiol	10.1128/jcm.22.2.212-215.1985	1985
Phylogeny	Francisella philomiragia comb. nov. (formerly Yersinia philomiragia) and Francisella tularensis biogroup novicida (formerly Francisella novicida) associated with human disease.	Hollis DG, Weaver RE, Steigerwalt AG, Wenger JD, Moss CW, Brenner DJ.	J Clin Microbiol	10.1128/jcm.27.7.1601-1608.1989	1989
	Cellular fatty acid composition of Francisella tularensis.	Jantzen E, Berdal BP, Omland T.	J Clin Microbiol	10.1128/jcm.10.6.928-930.1979	1979
Enzymology	A real-time PCR array for hierarchical identification of Francisella isolates.	Svensson K, Granberg M, Karlsson L, Neubauerova V, Forsman M, Johansson A.	PLoS One	10.1371/journal.pone.0008360	2009
Phylogeny	Proteogenomic biomarkers for identification of Francisella species and subspecies by matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.	Durighello E, Bellanger L, Ezan E, Armengaud J	Anal Chem	10.1021/ac501840g	2014
Pathogenicity	Decontamination of a hospital room using gaseous chlorine dioxide: Bacillus anthracis, Francisella tularensis, and Yersinia pestis.	Lowe JJ, Gibbs SG, Iwen PC, Smith PW, Hewlett AL	J Occup Environ Hyg	10.1080/15459624.2013.818241	2013
	Monoclonal antibodies reacting specifically with Francisella sp.	Greiser-Wilke I, Soine C, Moennig V	Zentralbl Veterinarmed B	10.1111/j.1439-0450.1989.tb00650.x	1989
Pathogenicity	Discrimination of human pathogenic subspecies of Francisella tularensis by using restriction fragment length polymorphism.	Thomas R, Johansson A, Neeson B, Isherwood K, Sjostedt A, Ellis J, Titball RW	J Clin Microbiol	10.1128/JCM.41.1.50-57.2003	2003
Cultivation	Effect of culture media and incubation temperature on growth of selected strains of Francisella tularensis.	Payne MP, Morton RJ	J Vet Diagn Invest	10.1177/104063879200400307	1992

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#44486	Culture Collection University of Gothenburg (CCUG) ; Curators of the CCUG; CCUG 2112
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#124043	Isabel Schober, Julia Koblitz: Data extracted from sequence databases, automatically matched based on designation and taxonomy .
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Francisella tularensis (CCUG 2112, ATCC 6223)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Physiology and metabolism

Oxygen tolerance

Spore formation

Isolation, sampling and environmental information

Interaction and safety

Sequence information

Genome sequences

16S sequences

Genome-based predictions

Predictions

Literature

Literature

References

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