Name: Blastomonas natatoria 1
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 14141

Type strain:

Species: Blastomonas natatoria

Strain Designation: 1, EY4220

Culture col. no.: DSM 3183, ATCC 35951, UQM 2507, JCM 12333, ACM 2507, CECT 4273, CIP 106842, IFO 15649, JCM 10396, KCTC 2886, LMG 17322, NBRC 15649, NCIMB 12085

Strain history: CIP <- 2000, E. Yabuuchi, Osaka, Japan: strain EY4220 <- ATCC <- UQM

NCBI tax ID(s): 34015 (species)

SI-ID 7893

LMG 14902, ATCC 35951, LMG 17322, ACM 2507, DSM 3183, IFO 15649, JCM 10396, NCIMB 12085, CIP 106842, CECT 4273, KCTC 2886, KCTC 2977, NBRC 15649, HAMBI 2081, JCM 12333, DSM 3138

Section

Blastomonas natatoria 1 is an obligate aerobe, mesophilic, Gram-negative bacterium that was isolated from freshwater swimming pool.

Gram-negative
mesophilic
obligate aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Sphingomonadales*
	Family	*Sphingomonadaceae*
	Genus	*Blastomonas*
	Species	**Blastomonas natatoria**
	Full Scientific Name (LPSN)	Blastomonas natatoria (Sly 1985) Sly and Cahill 1997

	Synonym	Sphingomonas natatoria
	Synonym	Blastobacter natatorius

Information on morphological and physiological properties Morphology

[Ref.: #121241]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %100

[Ref.: #121241]	Cell shape	rod-shaped

[Ref.: #121241]	Motility	yes

[Ref.: #121241]	Pigment production	no
[Ref.: #121241]	Pigment name	Pyocyanin

Information on culture and growth conditions Culture and growth conditions

[Ref.: #1165]

Culture medium

YPGA (DSMZ Medium 1015)

[Ref.: #1165]

Culture medium growth

[Ref.: #1165]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1165]

Culture medium composition

expand / minimize

[Ref.: #1165]

Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

[Ref.: #1165]

Culture medium growth

[Ref.: #1165]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1165]

Culture medium composition

expand / minimize

[Ref.: #1165]

Culture medium

PYEA MEDIUM (DSMZ Medium 364)

[Ref.: #1165]

Culture medium growth

[Ref.: #1165]

Culture medium link

Medium recipe at MediaDive

[Ref.: #1165]

Culture medium composition

expand / minimize

[Ref.: #37287]

Culture medium

MEDIUM 72- for trypto casein soja agar

[Ref.: #37287]

Culture medium growth

[Ref.: #37287]

Culture medium composition

expand / minimize

[Ref.: #121241]

Culture medium

CIP Medium 72

[Ref.: #121241]

Culture medium growth

[Ref.: #121241]

Culture medium link

Medium recipe provided by DSMZ

	Kind of temperature		Temperature
[Ref.: #1165]		growth	28 °C
[Ref.: #37287]		growth	25 °C
[Ref.: #67770]		growth	28 °C
[Ref.: #121241]		growth	25-37 °C
[Ref.: #121241]	no	growth	5 °C
[Ref.: #121241]	no	growth	10 °C
[Ref.: #121241]	no	growth	41 °C

[Ref.: #1165]	Temperature range	mesophilic
[Ref.: #37287]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #121241]	Temperature range	mesophilic
[Ref.: #121241]	Temperature range	psychrophilic
[Ref.: #121241]	Temperature range	thermophilic

Information on physiology and metabolism Physiology and metabolism

[Ref.: #121241]

Oxygen tolerance

obligate aerobe

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.996

	Salt	Tested relation		Salt conc.
[Ref.: #121241]	NaCl		growth	0-4	%
[Ref.: #121241]	NaCl	no	growth	6	%
[Ref.: #121241]	NaCl	no	growth	8	%
[Ref.: #121241]	NaCl	no	growth	10	%

[Ref.: #67770]

Observation

quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #121241]	esculin ChEBI	-	hydrolysis
[Ref.: #121241]	nitrate ChEBI	-	reduction
[Ref.: #121241]	nitrate ChEBI	-	respiration
[Ref.: #121241]	nitrite ChEBI	-	reduction

	Antibiotica	Metabolite	Antibiotic sensitivity	Antibiotic resistance
[Ref.: #121241]		0129 (2,4-Diamino-6,7-di-iso-propylpteridine phosphate)	yes	no

	Metabolite production	Metabolite	Production
[Ref.: #121241]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #68382]	acid phosphatase	+	3.1.3.2	* from API zym
[Ref.: #121241]	alcohol dehydrogenase	-	1.1.1.1
[Ref.: #68382]	alkaline phosphatase	+	3.1.3.1	* from API zym
[Ref.: #68382]	alpha-chymotrypsin	-	3.4.21.1	* from API zym
[Ref.: #68382]	alpha-fucosidase	-	3.2.1.51	* from API zym
[Ref.: #68382]	alpha-galactosidase	-	3.2.1.22	* from API zym
[Ref.: #68382]	alpha-glucosidase	+	3.2.1.20	* from API zym
[Ref.: #68382]	alpha-mannosidase	-	3.2.1.24	* from API zym
[Ref.: #121241]	amylase	-
[Ref.: #121241]	beta-galactosidase	-	3.2.1.23
[Ref.: #68382]	beta-galactosidase	-	3.2.1.23	* from API zym
[Ref.: #68382]	beta-glucosidase	-	3.2.1.21	* from API zym
[Ref.: #68382]	beta-glucuronidase	+	3.2.1.31	* from API zym
[Ref.: #121241]	caseinase	-	3.4.21.50
[Ref.: #121241]	catalase	+	1.11.1.6
[Ref.: #68382]	cystine arylamidase	-	3.4.11.3	* from API zym
[Ref.: #121241]	DNase	-
[Ref.: #68382]	esterase (C 4)	+		* from API zym
[Ref.: #68382]	esterase lipase (C 8)	+		* from API zym
[Ref.: #121241]	gelatinase	+/-
[Ref.: #121241]	lecithinase	-
[Ref.: #68382]	leucine arylamidase	+	3.4.11.1	* from API zym
[Ref.: #121241]	lipase	-
[Ref.: #68382]	lipase (C 14)	-		* from API zym
[Ref.: #121241]	lysine decarboxylase	-	4.1.1.18
[Ref.: #68382]	N-acetyl-beta-glucosaminidase	-	3.2.1.52	* from API zym
[Ref.: #68382]	naphthol-AS-BI-phosphohydrolase	+		* from API zym
[Ref.: #121241]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #121241]	oxidase	+
[Ref.: #121241]	protease	-
[Ref.: #68382]	trypsin	+	3.4.21.4	* from API zym
[Ref.: #121241]	tryptophan deaminase	-
[Ref.: #121241]	tween esterase	+
[Ref.: #121241]	urease	-	3.5.1.5
[Ref.: #68382]	valine arylamidase	-		* from API zym
	Only first 10 entries are displayed. Click here to see all.

API® Biotype100:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	ControlQ	D-glucose as carbon sourceGLU	D-fructose as carbon sourceFRU	D-galactose as carbon sourceGAL	D-trehalose as carbon sourceTRE	D-mannose as carbon sourceMNE	L-sorbose as carbon sourceSBE	D-melibiose as carbon sourceMEL	sucrose as carbon sourceSAC	D-raffinose as carbon sourceRAF	maltotriose as carbon sourceMTE	maltose as carbon sourceMAL	lactose as carbon sourceLAC	lactulose as carbon sourceLTE	1-O-methyl-beta-galactopyranoside as carbon sourceMbGa	1-O-methyl-alpha-galactopyranoside as carbon sourceMaGa	D-cellobiose as carbon sourceCEL	gentiobiose as carbon sourceGEN	1-O-methyl-beta-D-glucopyranoside as carbon sourceMbGu	esculin as carbon sourceESC	D-ribose as carbon sourceRIB	L-arabinose as carbon sourceARA	D-xylose as carbon sourceXYL	palatinose as carbon sourcePLE	L-rhamnose as carbon sourceRHA	L-fucose as carbon sourceFUC	D-melezitose as carbon sourceMLZ	D-arabitol as carbon sourceDARL	L-arabitol as carbon sourceLARL	xylitol as carbon sourceXLT	dulcitol as carbon sourceDUL	D-tagatose as carbon sourceTAG	glycerol as carbon sourceGLY	myo-inositol as carbon sourceINO	D-mannitol as carbon sourceMAN	maltitol as carbon sourceMTL	D-turanose as carbon sourceTUR	D-sorbitol as carbon sourceSOR	adonitol as carbon sourceADO	hydroxyquinoline-beta-glucuronide as carbon sourceHBG	D-lyxose as carbon sourceLYX	i-erythritol as carbon sourceERY	1-O-methyl-alpha-D-glucoside as carbon sourceMDG	3-O-methyl-D-glucose as carbon source3MDG	D-saccharate as carbon sourceSAT	mucate as carbon sourceMUC	L-tartrate as carbon sourceLTAT	D-tartrate as carbon sourceDTAT	meso-tartrate as carbon sourceMTAT	D-malate as carbon sourceDMLT	L-malate as carbon sourceLMLT	cis-aconitate as carbon sourceCATE	trans-aconitate as carbon sourceTATE	tricarballylate as carbon sourceTTE	citrate as carbon sourceCIT	D-glucuronate as carbon sourceGRT	D-galacturonate as carbon sourceGAT	2-ketogluconate as carbon source2KG	5-ketogluconate as carbon source5KG	tryptophan as carbon sourceTRY	N-acetyl-D-glucosamine as carbon sourceNAG	D-gluconate as carbon sourceGNT	phenylacetate as carbon sourcePAC	protocatechuate as carbon sourcePAT	4-hydroxybenzoate as carbon sourcepOBE	quinate as carbon sourceQAT	gentisate as carbon sourceGTE	3-hydroxybenzoate as carbon sourcemOBE	benzoate as carbon sourceBAT	3-phenylpropionate as carbon sourcePPAT	m-coumarate as carbon sourceCMT	trigonelline as carbon sourceTGE	betaine as carbon sourceBET	putrescine as carbon sourcePCE	4-aminobutyrate as carbon sourceABT	histamine as carbon sourceHIN	DL-lactate as carbon sourceLAT	caprate as carbon sourceCAP	caprylate as carbon sourceCYT	L-histidine as carbon sourceHIS	succinate as carbon sourceSUC	fumarate as carbon sourceFUM	glutarate as carbon sourceGRE	DL-glycerate as carbon sourceGYT	5-aminovalerate as carbon sourceAVT	ethanolamine as carbon sourceETN	tryptamine as carbon sourceTTN	D-glucosamine as carbon sourceGLN	itaconate as carbon sourceITA	3-hydroxybutyrate as carbon source3OBU	L-aspartate as carbon sourceAPT	L-glutamate as carbon sourceGTT	L-proline as carbon sourcePRO	D-alanine as carbon sourceDALA	L-alanine as carbon sourceLALA	L-serine as carbon sourceSER	malonate as carbon sourceMNT	propionate as carbon sourcePROP	L-tyrosine as carbon sourceTYR	2-ketoglutarate as carbon source2KT
[Ref.: #121241]	1871	not determinedn.d.	-	-	-	-	-	-	-	+	-	+	+	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	+	-	-	-	-	-	-	-	-

API® zym:

Note that the displayed test results represent raw data and therefore may deviate!

	API ID	Control	2-naphthyl phosphateAlkaline phosphatase	2-naphthyl butyrateEsterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	2-naphthyl myristateLipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	N-benzoyl-DL-arginine-2-naphthylamideTrypsin	N-glutaryl-phenylalanine-2-naphthylamidealpha- Chymotrypsin	2-naphthyl phosphateAcid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	6-Br-2-naphthyl-alphaD-galactpyranosidealpha- Galactosidase	2-naphthyl-betaD-galactpyranosidebeta- Galactosidase	Naphthol-AS-BI-betaD-glucuronidebeta- Glucuronidase	2-naphthyl-alphaD-glucopyranosidealpha- Glucosidase	6-Br-2-naphthyl-betaD-glucopyranosidebeta- Glucosidase	1-naphthyl-N-acetyl-betaD-glucosaminideN-acetyl-beta- glucosaminidase	6-Br-2-naphthyl-alphaD-mannopyranosidealpha- Mannosidase	2-naphthyl-alphaL-fucopyranosidealpha- Fucosidase
[Ref.: #121241]	9853	-	+	+	+	-	+	-	-	+	-	+	+	-	-	+	+	-	-	-	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #1165]	Sample type/isolated from	freshwater swimming pool

[Ref.: #67770]	Sample type/isolated from	Freshwater swimming pool

[Ref.: #121241]	Sample type/isolated from	Environment, Fresh water, swimming pool
[Ref.: #121241]	Country	Australia
[Ref.: #121241]	Country ISO 3 Code	AUS
[Ref.: #121241]	Continent	Australia and Oceania
* marker position based on {}

Isolation sources categories

#Engineered	#Built environment	#Water reservoir (Aquarium/pool)
#Environmental	#Aquatic	#Freshwater

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence Y13774 (>99% sequence identity) for Blastomonas from Microbeatlas

Aquatic Samples	21935
Soil Samples	10685
Animal Samples	16529
Plant Samples	4469
Total Samples	53618

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #1165]	Biosafety level	1	Risk group (German classification) According to TRBA 466
[Ref.: #121241]	Biosafety level	1	Risk group (French classification)

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #20218]	Blastomonas natatoria 16S ribosomal RNA gene, partial sequence; 16S-23S ribosomal RNA intergenic spacer, complete sequence; and 23S ribosomal RNA gene, partial sequence	GQ907186	679	ENA	34015 tax ID	*
[Ref.: #20218]	Sphingomonas natatoria gene for 16S ribosomal RNA	AB024288	1477	ENA	34015 tax ID	*
[Ref.: #20218]	Sphingomonas phyllosphaerae strain DSM 3183 16S ribosomal RNA gene, partial sequence	FJ999662	1450	ENA	257003 tax ID	*
[Ref.: #20218]	Blastomonas natatoria 16S rRNA gene, partial	Y13774	1421	ENA	34015 tax ID	*
[Ref.: #20218]	Blastomonas natatoria gene for 16S rRNA, partial sequence, strain: NBRC 15649	AB680933	1414	ENA	34015 tax ID	*

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Blastomonas natatoria DSM 3183	GCA_003201955	scaffold	GenBank	34015 tax ID
[Ref.: #66792]	Blastomonas natatoria strain DSM 3183	34015.5	wgs	PATRIC	34015 tax ID	*
[Ref.: #66792]	Blastomonas natatoria DSM 3183	2756170222	draft	JGI IMG/M	34015 tax ID	*

[Ref.: #1165]	GC-content	65.0 mol%
[Ref.: #67770]	GC-content	65 mol%	thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Blastomonas natatoria DSM 3183 NCBI: GCA_003201955.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Blastomonas natatoria strain DSM 3183 PATRIC: 34015.5
[Ref.: #69480]	gram-positive	no	97.438	no
[Ref.: #69480]	anaerobic	no	99.043	no
[Ref.: #69480]	halophile	no	92.27	no
[Ref.: #69480]	spore-forming	no	94.364	no
[Ref.: #69480]	glucose-util	yes	87.937	no
[Ref.: #69480]	motile	yes	62.042	no
[Ref.: #69480]	flagellated	no	86.982	no
[Ref.: #69480]	aerobic	yes	94.453	no
[Ref.: #69480]	thermophile	no	96.768	yes
[Ref.: #69480]	glucose-ferment	no	92.299	no

Availability in culture collections External links

[Ref.: #1165]

Culture collection no.

DSM 3183, ATCC 35951, UQM 2507, JCM 12333, ACM 2507, CECT 4273, CIP 106842, IFO 15649, JCM 10396, KCTC 2886, LMG 17322, NBRC 15649, NCIMB 12085

[Ref.: #83306]

SI-ID 7893

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Blastomonas fulva sp. nov., aerobic photosynthetic bacteria isolated from a Microcystis culture.	Lee HG, Ko SR, Lee JW, Lee CS, Ahn CY, Oh HM, Jin L	Int J Syst Evol Microbiol	10.1099/ijsem.0.002084	2017	*
Phylogeny	Blastomonas aquatica sp. nov., a bacteriochlorophyll-containing bacterium isolated from lake water.	Xiao N, Liu Y, Liu X, Gu Z, Jiao N, Liu H, Zhou Y, Shen L	Int J Syst Evol Microbiol	10.1099/ijs.0.000153	2015	*
Phylogeny	Identification of newly zeaxanthin-producing bacteria isolated from sponges in the Gulf of Thailand and their zeaxanthin production.	Thawornwiriyanun P, Tanasupawat S, Dechsakulwatana C, Techkarnjanaruk S, Suntornsuk W	Appl Biochem Biotechnol	10.1007/s12010-012-9760-2	2012	*
Phylogeny	Emendation of the description of Blastomonas natatoria (Sly 1985) Sly and Cahill 1997 as an aerobic photosynthetic bacterium and reclassification of Erythromonas ursincola Yurkov et al. 1997 as Blastomonas ursincola comb. nov.	Hiraishi A, Kuraishi H, Kawahara K	Int J Syst Evol Microbiol	10.1099/00207713-50-3-1113	2000	*
Phylogeny	Proposal of Sphingomonas suberifaciens (van Bruggen, Jochimsen and Brown 1990) comb. nov., sphingomonas natatoria (Sly 1985) comb. nov., Sphingomonas ursincola (Yurkov et al. 1997) comb. nov., and emendation of the genus Sphingomonas.	Yabuuchi E, Kosako Y, Naka T, Suzuki S, Yano I	Microbiol Immunol	10.1111/j.1348-0421.1999.tb02414.x	1999	*

References

#1165

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 3183

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#20218

Verslyppe, B., De Smet, W., De Baets, B., De Vos, P., Dawyndt P.: StrainInfo introduces electronic passports for microorganisms.. Syst Appl Microbiol. 37: 42 - 50 2014 ( DOI 10.1016/j.syapm.2013.11.002 , PubMed 24321274 )

#37287 ; Curators of the CIP;

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#68382

Automatically annotated from API zym .

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#83306

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID7893.1 )

#121241 Collection of Institut Pasteur ; Curators of the CIP; CIP 106842
* These data were automatically processed and therefore are not curated

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Blastomonas natatoria DSM 3183

Blastomonas natatoria strain DSM 3183

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