Name: Micromonospora grosourdyae A-T 7972
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 141169

Type strain:

Species: Micromonospora grosourdyae

Strain Designation: A-T 7972

Culture col. no.: BCC 50981, TBRC 6031, NBRC 112512, DSM 114116

Strain history: <- TBRC; TBRC 6031

NCBI tax ID(s): 2047739 (species)

SI-ID 405167

DSM 114116

Section

Micromonospora grosourdyae A-T 7972 is an aerobe, spore-forming, Gram-positive bacterium that builds a substrate mycelium and was isolated from from wild orchid Grosourdya appendiculata.

Gram-positive
spore-forming
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micromonosporales*
	Family	*Micromonosporaceae*
	Genus	*Micromonospora*
	Species	**Micromonospora grosourdyae**
	Full Scientific Name (LPSN)	Micromonospora grosourdyae Carro and Oren 2022

Synonym

Verrucosispora endophytica

Information on morphological and physiological properties Morphology

[Ref.: #44089]	Gram stain	positive

[Ref.: #44089]	Colony color	orange
[Ref.: #44089]	Cultivation medium used	ISP 2

[Ref.: #44089]	Multicellular complex forming ability	yes
[Ref.: #44089]	Multicellular complex name	substrate mycelium

Multimedia content

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Caption:

Medium 65, 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe provided by DSMZ

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Culture medium

ISP 2

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Culture medium growth

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Culture medium

ISP 3

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Culture medium growth

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Culture medium

ISP 7

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Culture medium growth

		Temperature
[Ref.: #44089]	growth	20-37 °C
[Ref.: #44089]	optimum	25-30 °C
[Ref.: #69026]	growth	28 °C

[Ref.: #44089]	Temperature range	mesophilic
[Ref.: #69026]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #44089]			optimum	9-10
[Ref.: #44089]			growth	7-11

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

[Ref.: #44089]	Spore description	single non-motile spores with warty surface formed at the tip of sporophores on substrate mycelia
[Ref.: #44089]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #44089]		NaCl		growth	0-3	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #44089]	D-fructose ChEBI	+	assimilation
[Ref.: #44089]	D-galactose ChEBI	-	assimilation
[Ref.: #44089]	D-glucose ChEBI	+	assimilation
[Ref.: #44089]	D-mannitol ChEBI	+	assimilation
[Ref.: #44089]	D-mannose ChEBI	+	assimilation
[Ref.: #44089]	D-ribose ChEBI	-	assimilation
[Ref.: #44089]	D-sorbitol ChEBI	-	assimilation
[Ref.: #44089]	D-xylose ChEBI	+	assimilation
[Ref.: #44089]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #44089]	L-arabinose ChEBI	+	assimilation
[Ref.: #44089]	L-arabitol ChEBI	-	assimilation
[Ref.: #44089]	L-rhamnose ChEBI	+	assimilation
[Ref.: #44089]	L-sorbose ChEBI	-	assimilation
[Ref.: #44089]	lactose ChEBI	-	assimilation
[Ref.: #44089]	maltose ChEBI	+	assimilation
[Ref.: #44089]	myo-inositol ChEBI	+	assimilation
[Ref.: #44089]	nitrate ChEBI	-	reduction
[Ref.: #44089]	raffinose ChEBI	-	assimilation
[Ref.: #44089]	starch ChEBI	+	hydrolysis
[Ref.: #44089]	sucrose ChEBI	+	assimilation
[Ref.: #44089]	trehalose ChEBI	+	assimilation
[Ref.: #44089]	xanthine ChEBI	-	hydrolysis
[Ref.: #44089]	xylitol ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #44089]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #44089]	alpha-glucosidase	+	3.2.1.20
[Ref.: #44089]	beta-glucosidase	+	3.2.1.21
[Ref.: #44089]	catalase	+	1.11.1.6
[Ref.: #44089]	esterase (C 4)	+
[Ref.: #44089]	leucine arylamidase	+	3.4.11.1
[Ref.: #44089]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #44089]	trypsin	+	3.4.21.4

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44089]

Fatty acid	Percentage
C16:0 iso	33.12
C15:0 iso	18.37
C17:1ω8c	9.1
C17:0 anteiso	6.92
C15:0 anteiso	5.02
C17:0 iso	3.15
C18:1ω9c	3.02
C16:1 iso H	2.86
C17:1 anteiso ω9c	2
C17:0	1.39
C16:0	1.39
C18:0	1.14
C14:0 iso	0.88
C18:0 iso	0.31
C15:1ω6c	0.27
10-methyl C18:0 TBSA	0.17
C16:0 2OH	0.16
C14:0	0.15
C18:1 2OH	0.12
C12:0	0.08
C17:0 3OH	0.07
C19:0	0.04

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #44089]	Sample type/isolated from	from wild orchid Grosourdya appendiculata
[Ref.: #44089]	Geographic location (country and/or sea, region)	Nakhorn Ratchasima Province
[Ref.: #44089]	Country	Thailand
[Ref.: #44089]	Country ISO 3 Code	THA
[Ref.: #44089]	Continent	Asia
[Ref.: #44089]	Enrichment culture	humic acid-salts vitamin agar
[Ref.: #44089]	Enrichment culture composition	supplemented with 50mg/l of nalidixic acid, 100mg/l of cycloheximide and 2mg/l of terbinafine
[Ref.: #44089]	Enrichment culture duration	21 days
[Ref.: #44089]	Enrichment culture temperature	28 ̊C
[Ref.: #44089]	Isolation/enrichment procedure	The root was treated using surface sterilization technique by sequential immersion in 70% (v/v) ethanol for 5min and sodium hypochlorite solution (4% available chloride, freshly prepared) for 5min. Then the sample was washed with sterile distilled water three times to remove surface sterilization agents and soaked in 10% NaHCO3 solution for 10min to disrupt growth of endophytic fungi. One gram of sample was cut into small pieces with sterilized scissors and then crushed in 9ml sterile sodium dod

[Ref.: #69026]	Sample type/isolated from	Root of plant, Grosourdya appendiculata
[Ref.: #69026]	Geographic location (country and/or sea, region)	Nakhorn Ratchasima
[Ref.: #69026]	Country	Thailand
[Ref.: #69026]	Country ISO 3 Code	THA
[Ref.: #69026]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host

#Plants

#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #44089]	16S rRNA gene sequence	MF033262		GenBank

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GC-content

70.5 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

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Culture collection no.

BCC 50981, TBRC 6031, NBRC 112512, DSM 114116

[Ref.: #96877]

SI-ID 405167

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Verrucosispora endophytica sp. nov., isolated from the root of wild orchid (Grosourdya appendiculata (Blume) Rchb.f.).	Ngaemthao W, Pujchakarn T, Chunhametha S, Suriyachadkun C	Int J Syst Evol Microbiol	10.1099/ijsem.0.002425	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#44089

Wipaporn Ngaemthao, Tawanmol Pujchakarn, Suwanee Chunhametha and Chanwit Suriyachadkun: Verrucosispora endophytica sp. nov., isolated from the root of wild orchid (Grosourdya appendiculata (Blume) Rchb.f.). IJSEM 67: 5114 - 5119 2017 ( DOI 10.1099/ijsem.0.002425 , PubMed 29043951 )

#69026

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 114116

#96877

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405167.1 )

* These data were automatically processed and therefore are not curated

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