Name: Kocuria salina Hv14b
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 141150

Type strain:

Species: Kocuria salina

Strain Designation: Hv14b

Culture col. no.: DSM 28714, CECT 9229

Strain history: <- M. Camacho, IFAPA Centro Las Torres-Tomejil, Consejeria de Agricultura y Pesca, Alcalá del Rio (Sevilla), Spain; Hv14b

NCBI tax ID(s): 1929416 (species)

SI-ID 400974

DSM 28714

Section

Kocuria salina Hv14b is an aerobe, Gram-positive, coccus-shaped bacterium that forms circular colonies and was isolated from rhizosphere soil sample of the halophyte Arthrocnemum macrostachyum.

coccus-shaped
colony-forming
Gram-positive
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Micrococcaceae*
	Genus	*Kocuria*
	Species	**Kocuria salina**
	Full Scientific Name (LPSN)	Kocuria salina Camacho et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #44071]	Gram stain	positive
[Ref.: #44071]	Cell length	1.0-1.2 µm
[Ref.: #44071]	Cell width	0.8-1.0 µm
[Ref.: #44071]	Cell shape	coccus-shaped
[Ref.: #44071]	Motility	no

[Ref.: #44071]	Colony size	1 mm
[Ref.: #44071]	Colony color	pastel-orange
[Ref.: #44071]	Colony shape	circular
[Ref.: #44071]	Incubation period	2 days
[Ref.: #44071]	Cultivation medium used	Nutrient agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #44071]

Culture medium

Nutrient agar (NA)

[Ref.: #44071]

Culture medium growth

[Ref.: #64976]

Culture medium

TRYPTICASE SOY YEAST EXTRACT MEDIUM (DSMZ Medium 92)

[Ref.: #64976]

Culture medium growth

[Ref.: #64976]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64976]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #44071]			growth	5-40 °C
[Ref.: #64976]			growth	28 °C

[Ref.: #64976]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #44071]			growth	7.0-11.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44071]

Oxygen tolerance

aerobe

[Ref.: #44071]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #44071]		NaCl		growth	0-12	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #44071]	(S)-lactate ChEBI	-	oxidation
[Ref.: #44071]	2-hydroxybutyrate ChEBI	-	oxidation
[Ref.: #44071]	2-oxobutanoate ChEBI	+	oxidation
[Ref.: #44071]	2-oxoglutarate ChEBI	+	oxidation
[Ref.: #44071]	3-O-methyl-D-glucose ChEBI	-	oxidation
[Ref.: #44071]	4-hydroxyphenylacetic acid ChEBI	-	oxidation
[Ref.: #44071]	acetate ChEBI	+	oxidation
[Ref.: #44071]	acetoacetate ChEBI	+	oxidation
[Ref.: #44071]	beta-hydroxybutyrate ChEBI	-	oxidation
[Ref.: #44071]	bromosuccinate ChEBI	-	oxidation
[Ref.: #44071]	butyrate ChEBI	+	oxidation
[Ref.: #44071]	casein	-	degradation
[Ref.: #44071]	cellobiose ChEBI	+	oxidation
[Ref.: #44071]	citrate ChEBI	-	oxidation
[Ref.: #44071]	D-arabitol ChEBI	-	oxidation
[Ref.: #44071]	D-aspartate ChEBI	-	oxidation
[Ref.: #44071]	D-fructose ChEBI	-	oxidation
[Ref.: #44071]	D-fructose 6-phosphate ChEBI	-	oxidation
[Ref.: #44071]	D-galactose ChEBI	+	oxidation
[Ref.: #44071]	D-galacturonic acid ChEBI	+	oxidation
[Ref.: #44071]	D-gluconate ChEBI	+	oxidation
[Ref.: #44071]	D-glucose 6-phosphate ChEBI	+	oxidation
[Ref.: #44071]	D-glucuronate ChEBI	+	oxidation
[Ref.: #44071]	D-mannitol ChEBI	+	oxidation
[Ref.: #44071]	D-mannose ChEBI	+	oxidation
[Ref.: #44071]	D-saccharate ChEBI	-	oxidation
[Ref.: #44071]	D-serine ChEBI	-	oxidation
[Ref.: #44071]	D-sorbitol ChEBI	-	oxidation
[Ref.: #44071]	dextrin ChEBI	-	oxidation
[Ref.: #44071]	fusidate ChEBI	-	oxidation
[Ref.: #44071]	galactarate ChEBI	-	oxidation
[Ref.: #44071]	gamma-aminobutyric acid ChEBI	-	oxidation
[Ref.: #44071]	gelatin ChEBI	-	oxidation
[Ref.: #44071]	glycerol ChEBI	-	oxidation
[Ref.: #44071]	glycine-proline ChEBI	-	oxidation
[Ref.: #44071]	guanidinium chloride ChEBI	-	oxidation
[Ref.: #44071]	hypoxanthine ChEBI	-	degradation
[Ref.: #44071]	inosine ChEBI	+	oxidation
[Ref.: #44071]	L-alanine ChEBI	+	oxidation
[Ref.: #44071]	L-arginine ChEBI	-	oxidation
[Ref.: #44071]	L-aspartate ChEBI	-	oxidation
[Ref.: #44071]	L-fucose ChEBI	-	oxidation
[Ref.: #44071]	L-galactonic acid gamma-lactone ChEBI	-	oxidation
[Ref.: #44071]	L-histidine ChEBI	-	oxidation
[Ref.: #44071]	L-malate ChEBI	+	oxidation
[Ref.: #44071]	L-pyroglutamic acid ChEBI	-	oxidation
[Ref.: #44071]	L-serine ChEBI	-	oxidation
[Ref.: #44071]	lactose ChEBI	+	oxidation
[Ref.: #44071]	lithium chloride ChEBI	+	oxidation
[Ref.: #44071]	maltose ChEBI	+	oxidation
[Ref.: #44071]	melibiose ChEBI	+	oxidation
[Ref.: #44071]	methyl (R)-lactate ChEBI	-	oxidation
[Ref.: #44071]	methyl beta-D-glucopyranoside ChEBI	+	oxidation
[Ref.: #44071]	methyl pyruvate ChEBI	-	oxidation
[Ref.: #44071]	myo-inositol ChEBI	+	oxidation
[Ref.: #44071]	N-acetyl-beta-D-mannosamine ChEBI	-	oxidation
[Ref.: #44071]	N-acetylglucosamine ChEBI	+	oxidation
[Ref.: #44071]	n-acetylneuraminate ChEBI	-	oxidation
[Ref.: #44071]	nalidixic acid ChEBI	+	oxidation
[Ref.: #44071]	pectin ChEBI	+	oxidation
[Ref.: #44071]	potassium tellurite ChEBI	+	oxidation
[Ref.: #44071]	propionate ChEBI	+	oxidation
[Ref.: #44071]	quinate ChEBI	+	oxidation
[Ref.: #44071]	salicin ChEBI	+	oxidation
[Ref.: #44071]	sodium bromate ChEBI	+	oxidation
[Ref.: #44071]	sodium formate ChEBI	-	oxidation
[Ref.: #44071]	stachyose ChEBI	+	oxidation
[Ref.: #44071]	starch ChEBI	+	degradation
[Ref.: #44071]	sucrose ChEBI	+	oxidation
[Ref.: #44071]	trehalose ChEBI	+	oxidation
[Ref.: #44071]	turanose ChEBI	+	oxidation
[Ref.: #44071]	tyrosine ChEBI	-	degradation
[Ref.: #44071]	xanthine ChEBI	-	degradation
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #44071]	acid phosphatase	-	3.1.3.2
[Ref.: #44071]	alkaline phosphatase	-	3.1.3.1
[Ref.: #44071]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #44071]	alpha-fucosidase	-	3.2.1.51
[Ref.: #44071]	alpha-galactosidase	-	3.2.1.22
[Ref.: #44071]	alpha-glucosidase	-	3.2.1.20
[Ref.: #44071]	alpha-mannosidase	-	3.2.1.24
[Ref.: #44071]	beta-galactosidase	-	3.2.1.23
[Ref.: #44071]	beta-glucosidase	-	3.2.1.21
[Ref.: #44071]	beta-glucuronidase	-	3.2.1.31
[Ref.: #44071]	catalase	+	1.11.1.6
[Ref.: #44071]	cystine arylamidase	-	3.4.11.3
[Ref.: #44071]	cytochrome oxidase	-	1.9.3.1
[Ref.: #44071]	esterase	+
[Ref.: #44071]	esterase Lipase (C 8)	+
[Ref.: #44071]	leucine arylamidase	+	3.4.11.1
[Ref.: #44071]	lipase (C 14)	-
[Ref.: #44071]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #44071]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #44071]	trypsin	-	3.4.21.4
[Ref.: #44071]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44071]

Fatty acid	Percentage
C15:0 anteiso	49.7
C15:0 iso	31.3
C15:0 iso 2OH / C16:1ω7c	4.7
C14:0	1.9
C17:1 iso ω9c	1.5
C15:1ω6c	1.4
C17:0 anteiso	1.4
C17:1 anteiso ω9c	1.3
C14:0 iso	1.3
C16:0 iso	1
C17:0 iso	1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #44071]	Sample type/isolated from	rhizosphere soil sample of the halophyte Arthrocnemum macrostachyum
[Ref.: #44071]	Sampling date	2013
[Ref.: #44071]	Geographic location (country and/or sea, region)	Lebrija marshes, Seville
[Ref.: #44071]	Country	Spain
[Ref.: #44071]	Country ISO 3 Code	ESP
[Ref.: #44071]	Continent	Europe
[Ref.: #44071]	Geographic location	37.0747°/-6.2256°
[Ref.: #44071]	Enrichment culture	nutrient agar
[Ref.: #44071]	Enrichment culture composition	1g meat extract, 2g yeast extract, 5g peptone, 5g NaCl in 1 l distilled water, plus 16g/l agar with cycloheximide (100 mg/l), adjusted to pH 7.4
[Ref.: #44071]	Enrichment culture duration	3 days
[Ref.: #44071]	Enrichment culture temperature	28 ̊C
[Ref.: #44071]	Isolation/enrichment procedure	suspended and homogenised in sterile mineral buffer (0.5g K2HPO4, 0.2g MgSO4 . 7H2O, 0.1g NaCl and 0.05g CaCl2 in 1 l distilled water)

[Ref.: #64976]	Sample type/isolated from	marsh soil, rhizosphere of the halophyte Arthrocnemum macrostachyum
[Ref.: #64976]	Geographic location (country and/or sea, region)	Seville, Lebrija marshes
[Ref.: #64976]	Country	Spain
[Ref.: #64976]	Country ISO 3 Code	ESP
[Ref.: #64976]	Continent	Europe
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Host	#Plants	#Shrub (Scrub)
#Host Body-Site	#Plant	#Rhizosphere

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LT674162 (>99% sequence identity) for Kocuria from Microbeatlas

Aquatic Samples	794
Soil Samples	1344
Animal Samples	4476
Plant Samples	1054
Total Samples	7668

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64976]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64976]	Kocuria sp. Hv14b partial 16S rRNA gene, strain DSM 28714, isolate Hv14b	LT674162	1400	ENA	1929416 tax ID

[Ref.: #64976]

GC-content

73.1 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #64976]

Culture collection no.

DSM 28714, CECT 9229

[Ref.: #96870]

SI-ID 400974

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Kocuria salina sp. nov., an actinobacterium isolated from the rhizosphere of the halophyte Arthrocnemum macrostachyum and emended description of Kocuria turfanensis.	Camacho M, Redondo-Gomez S, Rodriguez-Llorente I, Rohde M, Sproer C, Schumann P, Klenk HP, Montero-Calasanz MDC	Int J Syst Evol Microbiol	10.1099/ijsem.0.002401	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#44071

Maria Camacho, Susana Redondo-Gomez, Ignacio Rodríguez-Llorente, Manfred Rohde, Cathrin Spröer, Peter Schumann, Hans-Peter Klenk and Maria del Carmen Montero-Calasanz: Kocuria salina sp. nov., an actinobacterium isolated from the rhizosphere of the halophyte Arthrocnemum macrostachyum and emended description of Kocuria turfanensis. IJSEM 67: 5006 - 5012 2017 ( DOI 10.1099/ijsem.0.002401 , PubMed 29034846 )

#64976

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 28714

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96870

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400974.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Kocuria salina in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF