Actibacterium pelagium (CGMCC 1.16012, KCTC 52653, DSM 109121)

Name: Actibacterium pelagium (CGMCC 1.16012, KCTC 52653, DSM 109121)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 141146

Type strain

Strain Designation JN33

Culture col. no. CGMCC 1.16012 KCTC 52653 DSM 109121 JN33

NCBI tax ID(s) 2029103

Special Collections DSMZ KCTC Literature strains

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Actibacterium pelagium JN33 is an aerobe, chemoorganotroph, Gram-negative prokaryote that forms circular colonies and was isolated from seawater.

Gram-negative rod-shaped colony-forming aerobe chemoorganotroph genome sequence 16S sequence

Name and taxonomic classification

SI-ID 404323

KCTC 52653,DSM 109121

@ref 20215

Last LPSN update 2025-12-16 10:03:46

Domain Pseudomonadati

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Rhodobacterales

Family Roseobacteraceae

Genus Actibacterium

Species Actibacterium pelagium

Full scientific name Actibacterium pelagium Guo et al. 2017

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
44067	negative	1.5-2.5 µm	0.6-1.2 µm	rod-shaped
125439	negative				97.3
125438	negative				98.833

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
44067	2 mm	cream	circular	3 days	MA agar

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
44067	Marine agar (MA)
64969	BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514)		Medium recipe at MediaDive	Name: BACTO MARINE BROTH (DIFCO 2216) (DSMZ Medium 514) Composition: NaCl 19.45 g/l MgCl2 5.9 g/l Bacto peptone 5.0 g/l Na2SO4 3.24 g/l CaCl2 1.8 g/l Yeast extract 1.0 g/l KCl 0.55 g/l NaHCO3 0.16 g/l Fe(III) citrate 0.1 g/l KBr 0.08 g/l SrCl2 0.034 g/l H3BO3 0.022 g/l Na2HPO4 0.008 g/l Na-silicate 0.004 g/l NaF 0.0024 g/l (NH4)NO3 0.0016 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
44067	positive	growth	10-35
44067	positive	optimum	30	mesophilic
64969	positive	growth	30	mesophilic
67771	positive	growth	30	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
44067	positive	optimum	7
44067	positive	growth	6.5-8.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance
44067	aerobe
67771	aerobe

Nutrition type

44067

Typechemoorganotroph

Spore formation

@ref	Spore formation	Confidence
44067
125439		95.2
125438		93.334

Halophily

@ref	Salt	Growth	Tested relation	Concentration
44067	NaCl		growth	0 %(w/v)
44067	Marine salts		growth	0 %(w/v)
44067	NaCl	positive	growth	0.5-5.0 %(w/v)
44067	NaCl	positive	optimum	3 %(w/v)

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
44067	17057 ChEBI	cellobiose	-	builds acid from
44067	17057 ChEBI	cellobiose	+	carbon source
44067	17057 ChEBI	cellobiose	+	energy source
44067	12936 ChEBI	D-galactose	-	builds acid from
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
44067	16899 ChEBI	D-mannitol	+	builds acid from
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
44067	16024 ChEBI	D-mannose	+	carbon source
44067	16024 ChEBI	D-mannose	+	energy source
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
44067	16988 ChEBI	D-ribose	-	builds acid from
44067	17924 ChEBI	D-sorbitol	+	builds acid from
44067	65327 ChEBI	D-xylose	+	builds acid from
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
44067	91260 ChEBI	disodium malate	-	carbon source
44067	91260 ChEBI	disodium malate	-	energy source
44067	4853 ChEBI	esculin	+	hydrolysis
44067	16236 ChEBI	ethanol	-	builds acid from
44067	16236 ChEBI	ethanol	+/-	carbon source
44067	16236 ChEBI	ethanol	+/-	energy source
44067	5291 ChEBI	gelatin	+	hydrolysis
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
44067	17234 ChEBI	glucose	+	builds acid from
44067	17234 ChEBI	glucose	+	carbon source
44067	17234 ChEBI	glucose	+	energy source
44067	17754 ChEBI	glycerol	+	carbon source
44067	17754 ChEBI	glycerol	+	energy source
44067	24898 ChEBI	isoleucine	+	carbon source
44067	24898 ChEBI	isoleucine	+	energy source
44067	16977 ChEBI	L-alanine	+	carbon source
44067	16977 ChEBI	L-alanine	+	energy source
44067	30849 ChEBI	L-arabinose	+	carbon source
44067	30849 ChEBI	L-arabinose	+	energy source
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
44067	17561 ChEBI	L-cysteine	-	carbon source
44067	17561 ChEBI	L-cysteine	-	energy source
44067	62345 ChEBI	L-rhamnose	-	builds acid from
44067	62345 ChEBI	L-rhamnose	+/-	carbon source
44067	62345 ChEBI	L-rhamnose	+/-	energy source
44067	17266 ChEBI	L-sorbose	-	builds acid from
44067	17266 ChEBI	L-sorbose	+/-	carbon source
44067	17266 ChEBI	L-sorbose	+/-	energy source
44067	17716 ChEBI	lactose	-	builds acid from
44067	17716 ChEBI	lactose	-	carbon source
44067	17716 ChEBI	lactose	-	energy source
68369	25115 ChEBI	malate	-	assimilation	from API 20NE
44067	17306 ChEBI	maltose	-	builds acid from
44067	17306 ChEBI	maltose	+/-	carbon source
44067	17306 ChEBI	maltose	+/-	energy source
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
44067	29864 ChEBI	mannitol	+	carbon source
44067	29864 ChEBI	mannitol	+	energy source
44067	6731 ChEBI	melezitose	-	builds acid from
44067	17268 ChEBI	myo-inositol	+/-	builds acid from
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
44067	17632 ChEBI	nitrate	-	reduction
68369	17632 ChEBI	nitrate	-	reduction	from API 20NE
44067	16634 ChEBI	raffinose	-	builds acid from
44067	16634 ChEBI	raffinose	+/-	carbon source
44067	16634 ChEBI	raffinose	+/-	energy source
44067	15963 ChEBI	ribitol	-	builds acid from
44067	32954 ChEBI	sodium acetate	+	carbon source
44067	32954 ChEBI	sodium acetate	+	energy source
44067	84997 ChEBI	sodium gluconate	+	carbon source
44067	84997 ChEBI	sodium gluconate	+	energy source
44067	28017 ChEBI	starch	-	hydrolysis
44067	17992 ChEBI	sucrose	+	carbon source
44067	17992 ChEBI	sucrose	+	energy source
44067	27082 ChEBI	trehalose	-	builds acid from
44067	27082 ChEBI	trehalose	-	carbon source
44067	27082 ChEBI	trehalose	-	energy source
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
44067	53424 ChEBI	tween 20	-	hydrolysis
44067	53423 ChEBI	tween 40	-	hydrolysis
44067	53426 ChEBI	tween 80	-	hydrolysis
68369	16199 ChEBI	urea	-	hydrolysis	from API 20NE

Antibiotic resistance

@ref	ChEBI	Metabolite	Sensitivity conc.	Resistance conc.
44067	2676	amoxicillin	20 µg (disc)
44067	28971	ampicillin	10 µg (disc)
44067	28669	bacitracin		0.04 Unit (disc)
44067	3393	carbenicillin	100 µg (disc)
44067	209807	cefoxitin	30 µg (disc)
44067	17698	chloramphenicol	30 µg (disc)
44067	48923	erythromycin	15 µg (disc)
44067	17833	gentamicin		10 µg (disc)
44067	6104	kanamycin	30 µg (disc)
44067	71415	nitrofurantoin	300 µg (disc)
44067	28368	novobiocin	30 µg (disc)
44067	7660	nystatin		100 µg (disc)
44067	17334	penicillin	10 µg (disc)
44067	8309	polymyxin b	300 Unit (disc)
44067	28077	rifampicin	5 µg (disc)
44067	17076	streptomycin		10 µg (disc)
44067	27902	tetracycline	30 µg (disc)
44067	28864	tobramycin	10 µg (disc)
44067	28001	vancomycin	30 µg (disc)

Metabolite production

@ref	Chebi-ID	Metabolite	Production
44067	35581 ChEBI	indole
68369	35581 ChEBI	indole		from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test
68369	35581 ChEBI	indole	-	from API 20NE

Enzymes

@ref	Value	Activity	Ec
44067	acid phosphatase	+	3.1.3.2
68382	acid phosphatase	-	3.1.3.2	from API zym
44067	alkaline phosphatase	+	3.1.3.1
68382	alkaline phosphatase	-	3.1.3.1	from API zym
44067	alpha-chymotrypsin	-	3.4.21.1
68382	alpha-chymotrypsin	-	3.4.21.1	from API zym
44067	alpha-fucosidase	-	3.2.1.51
68382	alpha-fucosidase	-	3.2.1.51	from API zym
44067	alpha-galactosidase	-	3.2.1.22
68382	alpha-galactosidase	-	3.2.1.22	from API zym
44067	alpha-glucosidase	-	3.2.1.20
68382	alpha-glucosidase	-	3.2.1.20	from API zym
44067	alpha-mannosidase	-	3.2.1.24
68382	alpha-mannosidase	-	3.2.1.24	from API zym
44067	arginine dihydrolase	-	3.5.3.6
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
44067	beta-galactosidase	-	3.2.1.23
68382	beta-galactosidase	-	3.2.1.23	from API zym
44067	beta-glucosidase	-	3.2.1.21
68382	beta-glucosidase	-	3.2.1.21	from API zym
44067	beta-glucuronidase	-	3.2.1.31
68382	beta-glucuronidase	-	3.2.1.31	from API zym
44067	catalase	+/-	1.11.1.6
44067	cystine arylamidase	-	3.4.11.3
68382	cystine arylamidase	-	3.4.11.3	from API zym
44067	cytochrome oxidase	+	1.9.3.1
44067	esterase (C 4)	+
44067	esterase Lipase (C 8)	+
68382	esterase lipase (C 8)	-		from API zym
68369	gelatinase	-		from API 20NE
68382	leucine arylamidase	+	3.4.11.1	from API zym
44067	lipase (C 14)	-
68382	lipase (C 14)	-		from API zym
44067	N-acetyl-beta-glucosaminidase	-	3.2.1.52
68382	N-acetyl-beta-glucosaminidase	-	3.2.1.52	from API zym
44067	naphthol-AS-BI-phosphohydrolase	+
68382	naphthol-AS-BI-phosphohydrolase	-		from API zym
44067	trypsin	-	3.4.21.4
68382	trypsin	-	3.4.21.4	from API zym
44067	urease	-	3.5.1.5
68369	urease	-	3.5.1.5	from API 20NE
44067	valine arylamidase	-
68382	valine arylamidase	-		from API zym

Fatty acid profile

Metadata FA analysis

@ref

44067

Fatty acid	Percentage
C18:1ω7c / C18:1ω6c	66.9
C16:0	11.3
C18:0 iso	5.8
11-Methyl C18:1ω7c	4.8
C10:0 3OH	4.6
C18:0	4.6
C16:1ω7c / C16:1ω6c	0.7
C20:1ω7c	0.5

API zym

@ref	Control	Alkaline phosphatase	Esterase (C 4)	2-naphtyl caprylateEsterase Lipase (C 8)	Lipase (C 14)	L-leucyl-2-naphthylamideLeucine arylamidase	L-valyl-2-naphthylamideValine arylamidase	L-cystyl-2-naphthylamideCystine arylamidase	Trypsin	alpha- Chymotrypsin	Acid phosphatase	Naphthol-AS-BI-phosphateNaphthol-AS-BI-phosphohydrolase	alpha- Galactosidase	beta- Galactosidase	beta- Glucuronidase	alpha- Glucosidase	beta- Glucosidase	N-acetyl-beta- glucosaminidase	alpha- Mannosidase	alpha- Fucosidase
64969	-	-	+/-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
64969	-	-	-	-	-	+/-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	not determinedn.d.

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Marine

Isolation

@ref	Sample type	Sampling date	Geographic location	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Country	Country ISO 3 Code	Continent	Latitude	Longitude
44067	seawater	2015-12	western Pacific Ocean	natural seawater agar	supplemented with 0.05% peptone, 0.01% yeast extract, 1.5% agar, pH 7.2-7.4	30 days	25
64969	seawater sample		western Pacific Ocean (3° N, 130 ° E)					International waters
67771	From seawater		western Pacific					China	CHN	Asia	3	130 3/130

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
64969	1	Risk group (German classification)

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
66792	ASM1464435v1 assembly for Actibacterium pelagium CGMCC 1.16012	contig	GCA_014644355	2029103.7	8065873894	2029103	78.05
66792	ASM228541v1 assembly for Actibacterium pelagium JN33	scaffold	GCA_002285415	2029103.3	2832254508	2029103	77.93

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
64969	Actibacterium pelagium strain JN33 16S ribosomal RNA gene, partial sequence	KY273602	1456		2029103

GC content

@ref	GC-content (mol%)	Method
44067	57.8	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Actibacterium pelagium JN33
NCBI: GCA_002285415

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	no	95.20	no
125439	motility	BacteriaNetⓘ	yes	66.30	no
125439	gram_stain	BacteriaNetⓘ	negative	97.30	no
125439	oxygen_tolerance	BacteriaNetⓘ	aerobe	89.60	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Actibacterium pelagium JN33
NCBI: GCA_002285415.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	98.83	yes
125438	anaerobic	anaerobicⓘ	no	93.34	yes
125438	aerobic	aerobicⓘ	yes	82.09	yes
125438	spore-forming	spore-formingⓘ	no	93.33	no
125438	thermophilic	thermophileⓘ	no	93.96	yes
125438	flagellated	motile2+ⓘ	no	76.89	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Actibacterium pelagium sp. nov., a novel alphaproteobacterium, and emended description of the genus Actibacterium.	Guo LL, Wu YH, Xu XW, Huang CJ, Xu YY, Cheng H, Wang CS, Wu M, Xu XW	Int J Syst Evol Microbiol	10.1099/ijsem.0.002417	2017

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#44067	Li-Li Guo, Yue-Hong Wu, Xiao-Wu Xu, Chao-Jie Huang, Yun-Yun Xu, Hong Cheng, Chun-Sheng Wang, Min Wu and Xue-Wei Xu: Actibacterium pelagium sp. nov., a novel alphaproteobacterium, and emended description of the genus Actibacterium. IJSEM 67: 5080 - 5086 2017 ( DOI 10.1099/ijsem.0.002417 , PubMed 29039308 )
#64969	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 109121
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#67771	Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
#68369	Automatically annotated from API 20NE .
#68382	Automatically annotated from API zym .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Actibacterium pelagium (CGMCC 1.16012, KCTC 52653, DSM 109121)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

Fatty acid profile?

API zym

API 20NE

Isolation, sampling and environmental information

Isolation source categories

Isolation

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_014644355

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media

Fatty acid profile