Name: Halomicrobium urmianum DC8
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 141118

Type strain:

Species: Halomicrobium urmianum

Strain Designation: DC8

Culture col. no.: IBRC-M 10911, CECT 8793

NCBI tax ID(s): 1586233 (species)

Section

Halomicrobium urmianum DC8 is an aerobe, Gram-negative, pleomorphic-shaped archaeon that was isolated from brine sample of a hypersaline lake.

Gram-negative
pleomorphic-shaped
aerobe
16S sequence
Archaea

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Halobacteria*
	Order	*Halobacteriales*
	Family	*Haloarculaceae*
	Genus	*Halomicrobium*
	Species	**Halomicrobium urmianum**
	Full Scientific Name (LPSN)	Halomicrobium urmianum (Mehrshad et al. 2016) Xin et al. 2022

Synonym

Halosiccatus urmianus

Information on morphological and physiological properties Morphology

[Ref.: #44030]	Gram stain	negative

[Ref.: #44030]	Cell length	1.1-1.5 µm

[Ref.: #44030]	Cell width	0.3-0.7 µm

[Ref.: #44030]	Cell shape	pleomorphic-shaped

[Ref.: #44030]	Motility	no

[Ref.: #44030]	Colony size	1.0-2.0 mm
[Ref.: #44030]	Colony color	pale orange
[Ref.: #44030]	Cultivation medium used	MGM liquid medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #44030]

Culture medium

MGM

[Ref.: #44030]

Culture medium growth

[Ref.: #44030]

Culture medium

MGM liquid medium

[Ref.: #44030]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #44030]			growth	25-55 °C
[Ref.: #44030]			optimum	45 °C

[Ref.: #44030]

Temperature range

thermophilic

	pH	Kind of pH		pH
[Ref.: #44030]			optimum	7.5
[Ref.: #44030]			growth	7.5-8.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44030]

Oxygen tolerance

aerobe

[Ref.: #44030]

Halophily / tolerance level

extremely halophilic

	Salt		Salt conc.
[Ref.: #44030]	NaCl	growth	2.5-5.0	M
[Ref.: #44030]	MgCl2	minimum	0.02	M
[Ref.: #44030]	NaCl	optimum	4	M
[Ref.: #44030]	MgCl2	optimum	0.1	M

[Ref.: #44030]

Observation

Cells lyse in water

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #44030]	casein	-	hydrolysis
[Ref.: #44030]	D-fructose ChEBI	-	builds acid from
[Ref.: #44030]	D-fructose ChEBI	-	carbon source
[Ref.: #44030]	D-fructose ChEBI	-	energy source
[Ref.: #44030]	D-galactose ChEBI	-	builds acid from
[Ref.: #44030]	D-galactose ChEBI	+	carbon source
[Ref.: #44030]	D-galactose ChEBI	+	energy source
[Ref.: #44030]	D-glucose ChEBI	-	builds acid from
[Ref.: #44030]	D-glucose ChEBI	+	carbon source
[Ref.: #44030]	D-glucose ChEBI	+	energy source
[Ref.: #44030]	D-malate ChEBI	-	carbon source
[Ref.: #44030]	D-malate ChEBI	-	energy source
[Ref.: #44030]	D-mannitol ChEBI	-	builds acid from
[Ref.: #44030]	D-mannitol ChEBI	+	carbon source
[Ref.: #44030]	D-mannitol ChEBI	+	energy source
[Ref.: #44030]	D-mannose ChEBI	-	builds acid from
[Ref.: #44030]	D-mannose ChEBI	-	carbon source
[Ref.: #44030]	D-mannose ChEBI	-	energy source
[Ref.: #44030]	D-ribose ChEBI	-	builds acid from
[Ref.: #44030]	D-ribose ChEBI	-	carbon source
[Ref.: #44030]	D-ribose ChEBI	-	energy source
[Ref.: #44030]	D-xylose ChEBI	-	builds acid from
[Ref.: #44030]	D-xylose ChEBI	-	carbon source
[Ref.: #44030]	D-xylose ChEBI	-	energy source
[Ref.: #44030]	dioxygen ChEBI	+	electron acceptor
[Ref.: #44030]	dna ChEBI	-	hydrolysis
[Ref.: #44030]	esculin ChEBI	+	hydrolysis
[Ref.: #44030]	gelatin ChEBI	+	hydrolysis
[Ref.: #44030]	glucose ChEBI	-	fermentation
[Ref.: #44030]	glycerol ChEBI	-	builds acid from
[Ref.: #44030]	glycerol ChEBI	-	carbon source
[Ref.: #44030]	glycerol ChEBI	-	energy source
[Ref.: #44030]	glycine ChEBI	-	carbon source
[Ref.: #44030]	glycine ChEBI	-	energy source
[Ref.: #44030]	L-alanine ChEBI	-	carbon source
[Ref.: #44030]	L-alanine ChEBI	-	energy source
[Ref.: #44030]	L-arabinose ChEBI	-	builds acid from
[Ref.: #44030]	L-arabinose ChEBI	-	carbon source
[Ref.: #44030]	L-arabinose ChEBI	-	energy source
[Ref.: #44030]	L-arginine ChEBI	-	carbon source
[Ref.: #44030]	L-arginine ChEBI	-	energy source
[Ref.: #44030]	L-aspartate ChEBI	-	carbon source
[Ref.: #44030]	L-aspartate ChEBI	-	energy source
[Ref.: #44030]	L-cysteine ChEBI	-	carbon source
[Ref.: #44030]	L-cysteine ChEBI	-	energy source
[Ref.: #44030]	L-glutamate ChEBI	-	carbon source
[Ref.: #44030]	L-glutamate ChEBI	-	energy source
[Ref.: #44030]	L-lysine ChEBI	-	carbon source
[Ref.: #44030]	L-lysine ChEBI	-	energy source
[Ref.: #44030]	L-methionine ChEBI	-	carbon source
[Ref.: #44030]	L-methionine ChEBI	-	energy source
[Ref.: #44030]	L-ornithine ChEBI	-	carbon source
[Ref.: #44030]	L-ornithine ChEBI	-	energy source
[Ref.: #44030]	L-phenylalanine ChEBI	-	carbon source
[Ref.: #44030]	L-phenylalanine ChEBI	-	energy source
[Ref.: #44030]	L-tyrosine ChEBI	-	carbon source
[Ref.: #44030]	L-tyrosine ChEBI	-	energy source
[Ref.: #44030]	lactate ChEBI	-	carbon source
[Ref.: #44030]	lactate ChEBI	-	energy source
[Ref.: #44030]	lactose ChEBI	-	builds acid from
[Ref.: #44030]	lactose ChEBI	+	carbon source
[Ref.: #44030]	lactose ChEBI	+	energy source
[Ref.: #44030]	maltose ChEBI	-	builds acid from
[Ref.: #44030]	maltose ChEBI	-	carbon source
[Ref.: #44030]	maltose ChEBI	-	energy source
[Ref.: #44030]	nitrate ChEBI	-	reduction
[Ref.: #44030]	pyruvate ChEBI	+	carbon source
[Ref.: #44030]	pyruvate ChEBI	+	energy source
[Ref.: #44030]	starch ChEBI	-	hydrolysis
[Ref.: #44030]	succinate ChEBI	-	carbon source
[Ref.: #44030]	succinate ChEBI	-	energy source
[Ref.: #44030]	sucrose ChEBI	-	builds acid from
[Ref.: #44030]	sucrose ChEBI	-	carbon source
[Ref.: #44030]	sucrose ChEBI	-	energy source
[Ref.: #44030]	trehalose ChEBI	-	builds acid from
[Ref.: #44030]	trehalose ChEBI	-	carbon source
[Ref.: #44030]	trehalose ChEBI	-	energy source
[Ref.: #44030]	tween 20 ChEBI	-	hydrolysis
[Ref.: #44030]	tween 40 ChEBI	-	hydrolysis
[Ref.: #44030]	tween 60 ChEBI	+	hydrolysis
[Ref.: #44030]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #44030]	acetoin ChEBI	no
[Ref.: #44030]	hydrogen sulfide ChEBI	no
[Ref.: #44030]	indole ChEBI	yes

	Physiological tests	Metabolite	Voges-Proskauer-test	Methylred-test
[Ref.: #44030]		acetoin ChEBI	-
[Ref.: #44030]		glucose ChEBI		-

	Enzyme	Enzyme activity	EC number
[Ref.: #44030]	arginine dihydrolase	-	3.5.3.6
[Ref.: #44030]	beta-galactosidase	+	3.2.1.23
[Ref.: #44030]	catalase	+	1.11.1.6
[Ref.: #44030]	cytochrome oxidase	+	1.9.3.1
[Ref.: #44030]	lysine decarboxylase	-	4.1.1.18
[Ref.: #44030]	ornithine decarboxylase	-	4.1.1.17
[Ref.: #44030]	urease	-	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #44030]	Sample type/isolated from	brine sample of a hypersaline lake
[Ref.: #44030]	Geographic location (country and/or sea, region)	Urmia salt lake
[Ref.: #44030]	Country	Iran
[Ref.: #44030]	Country ISO 3 Code	IRN
[Ref.: #44030]	Continent	Asia
[Ref.: #44030]	Geographic location	37.5333°/45.7167°
[Ref.: #44030]	Enrichment culture	modified growth medium (MGM)
[Ref.: #44030]	Enrichment culture composition	This medium contained a 23 % (w/v) salt mixture prepared from 30 % (w/v) stock solution, which consisted of (g/l ): NaCl, 240; MgSO4 . 7H2O, 35; MgCl2 . 6H2O, 30; KCl, 7 and CaCl 2 . 2H2O; supplemented with 1 % (w/v) peptone (Merck) and 0.2 % (w/v) yeast extract (Merck); 1.5 % (w/v) agar; The pH of the medium was adjusted to pH 7.2–7.4 with 2 M Tris-base (Merck)
[Ref.: #44030]	Enrichment culture duration	2 months
[Ref.: #44030]	Enrichment culture temperature	40 ̊C
* marker position based on {}

Isolation sources categories

#Condition	#Saline	-
#Environmental	#Aquatic	#Lake (large)
#Environmental	#Aquatic	#Non-marine Saline and Alkaline

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KM596805 (>99% sequence identity) for Halosiccatus urmianus subclade from Microbeatlas

Aquatic Samples	12
Soil Samples	10
Animal Samples	41
Plant Samples	1
Total Samples	64

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #44030]	16S rRNA gene sequence	KM596805		GenBank

[Ref.: #44030]

GC-content

68.1 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #44030]

Culture collection no.

IBRC-M 10911, CECT 8793

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halosiccatus urmianus gen. nov., sp. nov., a haloarchaeon from a salt lake.	Mehrshad M, Amoozegar MA, Makhdoumi A, Fazeli SAS, Farahani H, Asadi B, Schumann P, Ventosa A	Int J Syst Evol Microbiol	10.1099/ijsem.0.000781	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#44030

Maliheh Mehrshad, Mohammad Ali Amoozegar, Ali Makhdoumi, Seyed Abolhassan Shahzadeh Fazeli, Homa Farahani, Basaer Asadi, Peter Schumann and Antonio Ventosa: Halosiccatus urmianus gen. nov., sp. nov., a haloarchaeon from a salt lake. IJSEM 66: 725 - 730 2016 ( DOI 10.1099/ijsem.0.000781 , PubMed 26596884 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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