Name: Rhabdanaerobium thermarum CGMCC 1.5219
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 141114

Type strain:

Species: Rhabdanaerobium thermarum

Culture col. no.: CGMCC 1.5219, KCTC 15623, DSM 105239, SYSU GA15001

Strain history: <- Wenjun Li, Sun Sat-Sen Univ.

NCBI tax ID(s): 1715347 (species)

SI-ID 405721

DSM 105239

Section

Rhabdanaerobium thermarum CGMCC 1.5219 is an anaerobe, Gram-positive, motile bacterium that was isolated from a hot spring.

Gram-positive
motile
rod-shaped
anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Eubacteriaceae*
	Genus	*Rhabdanaerobium*
	Species	**Rhabdanaerobium thermarum**
	Full Scientific Name (LPSN)	Rhabdanaerobium thermarum Liu et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #44022]	Gram stain	positive
[Ref.: #44022]	Cell shape	rod-shaped
[Ref.: #44022]	Motility	yes

[Ref.: #67771]	Gram stain	positive

[Ref.: #67771]	Cell shape	rod-shaped
[Ref.: #67771]	Motility	yes
[Ref.: #67771]	Flagellum arrangement	subpolar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64955]

Culture medium

ANAEROBIC FRESHWATER (FWM) MEDIUM (DSMZ Medium 503)

[Ref.: #64955]

Culture medium growth

[Ref.: #64955]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64955]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #44022]	growth	25-55 °C
[Ref.: #44022]	optimum	45 °C
[Ref.: #64955]	growth	45 °C
[Ref.: #67771]	growth	45 °C

[Ref.: #44022]	Temperature range	thermophilic
[Ref.: #64955]	Temperature range	thermophilic
[Ref.: #67771]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #44022]			optimum	7
[Ref.: #44022]			growth	6.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #67771]

Oxygen tolerance

anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #44022]		NaCl		growth	0.5-5.0	%
[Ref.: #44022]		NaCl		optimum	1.5	%

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #44022]	ammonium chloride ChEBI	+	nitrogen source
[Ref.: #44022]	beef extract	+	assimilation
[Ref.: #44022]	D-arabinose ChEBI	+	carbon source
[Ref.: #44022]	D-galactose ChEBI	+	carbon source
[Ref.: #44022]	D-mannose ChEBI	+	carbon source
[Ref.: #44022]	D-sorbitol ChEBI	+	carbon source
[Ref.: #44022]	esculin ChEBI	-	hydrolysis
[Ref.: #44022]	galactitol ChEBI	+	carbon source
[Ref.: #44022]	gelatin ChEBI	+	hydrolysis
[Ref.: #44022]	glucose ChEBI	+	carbon source
[Ref.: #44022]	glycine ChEBI	-	nitrogen source
[Ref.: #44022]	L-alanine ChEBI	+	nitrogen source
[Ref.: #44022]	L-arginine ChEBI	+	nitrogen source
[Ref.: #44022]	L-cysteine ChEBI	-	nitrogen source
[Ref.: #44022]	L-isoleucine ChEBI	-	nitrogen source
[Ref.: #44022]	L-leucine ChEBI	-	nitrogen source
[Ref.: #44022]	L-lysine ChEBI	-	nitrogen source
[Ref.: #44022]	L-proline ChEBI	+	nitrogen source
[Ref.: #44022]	L-rhamnose ChEBI	+	carbon source
[Ref.: #44022]	L-threonine ChEBI	+	nitrogen source
[Ref.: #44022]	lactose ChEBI	+	carbon source
[Ref.: #44022]	maltose ChEBI	+	carbon source
[Ref.: #44022]	methionine ChEBI	+	nitrogen source
[Ref.: #44022]	peptone	+	assimilation
[Ref.: #44022]	raffinose ChEBI	+	carbon source
[Ref.: #44022]	starch ChEBI	+	assimilation
[Ref.: #44022]	trehalose ChEBI	-	carbon source
[Ref.: #44022]	trehalose ChEBI	+	builds acid from
[Ref.: #44022]	xylitol ChEBI	+	carbon source
[Ref.: #44022]	yeast extract	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #44022]	amikacin ChEBI	yes	30	µg (disc)
[Ref.: #44022]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #44022]	ciprofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #44022]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #44022]	netilmycin ChEBI	yes	30	µg (disc)
[Ref.: #44022]	novobiocin ChEBI				yes	5	µg
[Ref.: #44022]	penicillin g ChEBI	yes	10	Unit
[Ref.: #44022]	polymyxin b ChEBI	yes	300	Unit
[Ref.: #44022]	streptomycin ChEBI				yes	10	µg
[Ref.: #44022]	tetracycline ChEBI	yes	30	µg (disc)
[Ref.: #44022]	tobramycin ChEBI	yes	10	µg (disc)
[Ref.: #44022]	vancomycin ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #44022]		indole ChEBI	no
[Ref.: #44022]		urea ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #44022]	arginine arylamidase	+
[Ref.: #44022]	catalase	-	1.11.1.6
[Ref.: #44022]	cytochrome oxidase	-	1.9.3.1
[Ref.: #44022]	leucine arylamidase	+	3.4.11.1
[Ref.: #44022]	phenylalanine arylamidase	-
[Ref.: #44022]	phenylalanine arylamidase	+

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #44022]	Sample type/isolated from	a hot spring
[Ref.: #44022]	Geographic location (country and/or sea, region)	Tengchong county, Yunnan province
[Ref.: #44022]	Country	China
[Ref.: #44022]	Country ISO 3 Code	CHN
[Ref.: #44022]	Continent	Asia

[Ref.: #64955]	Sample type/isolated from	hot spring
[Ref.: #64955]	Geographic location (country and/or sea, region)	Yunnan province, Tengchong county
[Ref.: #64955]	Country	China
[Ref.: #64955]	Country ISO 3 Code	CHN
[Ref.: #64955]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From hot spring
[Ref.: #67771]	Geographic location (country and/or sea, region)	Tengchong county, Yunnan province
[Ref.: #67771]	Country	China
[Ref.: #67771]	Country ISO 3 Code	CHN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Thermal spring
#Condition	#Thermophilic (>45°C)	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KR054687 (>99% sequence identity) for Rhabdanaerobium thermarum subclade from Microbeatlas

Aquatic Samples	128
Soil Samples	792
Animal Samples	507
Plant Samples	282
Total Samples	1709

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64955]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64955]	Rhabdanaerobium thermarum strain SYSU GA15001 16S ribosomal RNA gene, partial sequence	KR054687	1542	ENA	1715347 tax ID

[Ref.: #64955]	GC-content	35.6 mol%	high performance liquid chromatography (HPLC)
[Ref.: #67771]	GC-content	35.6 mol%

Availability in culture collections External links

[Ref.: #64955]

Culture collection no.

CGMCC 1.5219, KCTC 15623, DSM 105239, SYSU GA15001

[Ref.: #96860]

SI-ID 405721

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rhabdanaerobium thermarum gen. nov., sp. nov., a novel anaerobic bacterium isolated from a hot spring.	Liu L, Jiao JY, Salam N, Zhou EM, Fang BZ, Xian WD, Li MM, Ding YP, Li WJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.002335	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#44022

Lan Liu, Jian-Yu Jiao, Nimaichand Salam, En-Min Zhou, Bao-Zhu Fang, Wen-Dong Xian, Meng-Meng Li, Yi-Ping Ding, Wen-Jun Li: Rhabdanaerobium thermarum gen. nov., sp. nov., a novel anaerobic bacterium isolated from a hot spring. IJSEM 67: 4584 - 4588 2017 ( DOI 10.1099/ijsem.0.002335 , PubMed 28945534 )

#64955

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105239

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96860

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405721.1 )

* These data were automatically processed and therefore are not curated

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