Name: Frankia asymbiotica M16386
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 141096

Type strain:

Species: Frankia asymbiotica

Strain Designation: M16386

Culture col. no.: DSM 100626, CECT 9040, NRRL B-16386

Strain history: <- M. Gtari, Faculté des sciences de Tunis, Tunesia; M16386 <- M. P. Lechevalier

NCBI tax ID(s): 1834516 (species)

Special Collections

Section

Frankia asymbiotica M16386 is an aerobe, heterotroph, Gram-positive bacterium that builds multilocular sporangia and was isolated from Morella californica.

Gram-positive
aerobe
heterotroph
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Frankiales*
	Family	*Frankiaceae*
	Genus	*Frankia*
	Species	**Frankia asymbiotica**
	Full Scientific Name (LPSN)	Frankia asymbiotica Nouioui et al. 2017

Synonym

Pseudofrankia asymbiotica

Information on morphological and physiological properties Morphology

[Ref.: #44005]	Gram stain	positive

[Ref.: #44005]	Motility	no

[Ref.: #44005]	Colony color	unpigmented
[Ref.: #44005]	Cultivation medium used	basic Propionate

[Ref.: #44005]	Multicellular complex forming ability	yes
[Ref.: #44005]	Multicellular complex name	multilocular sporangia

[Ref.: #44005]	Multicellular complex name	hyphae

Information on culture and growth conditions Culture and growth conditions

[Ref.: #44005]

Culture medium

Basic Propionate

[Ref.: #44005]

Culture medium growth

[Ref.: #44005]

Culture medium composition

expand / minimize

[Ref.: #64973]

Culture medium

MODIFIED BAP+ (PAGFS) MEDIUM FOR FRANKIA (DSMZ Medium 1590)

[Ref.: #64973]

Culture medium growth

[Ref.: #64973]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64973]

Culture medium composition

expand / minimize

	Temperatures	Kind of temperature		Temperature
[Ref.: #64973]			growth	28 °C

	pH	Kind of pH		pH
[Ref.: #44005]			growth	6.3-6.8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #44005]

Oxygen tolerance

aerobe

[Ref.: #44005]	Nutrition type	heterotroph
[Ref.: #44005]	Nutrition type	chemoorganotroph
[Ref.: #44005]	Nutrition type	diazotroph

[Ref.: #44005]	Observation	characterized by branched substrate hyphae, vesicles
[Ref.: #44005]	Observation	the type strain is unable to nodulate its host plant of origin or any of the tested actinorhizal plant species
[Ref.: #44005]	Observation	the predominant menaquinones are MK-9(H 4 ) (56.8 %) and MK-9(H 6 ) (24.4 %)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #44005]	2-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #44005]	beta-hydroxybutyrate ChEBI	+	assimilation
[Ref.: #44005]	butyrate ChEBI	+	assimilation
[Ref.: #44005]	cellobiose ChEBI	+	assimilation
[Ref.: #44005]	D-gluconate ChEBI	+	assimilation
[Ref.: #44005]	D-glucose ChEBI	+	assimilation
[Ref.: #44005]	D-mannose ChEBI	+	assimilation
[Ref.: #44005]	D-rhamnose ChEBI	+	assimilation
[Ref.: #44005]	decanoate ChEBI	+	assimilation
[Ref.: #44005]	dinitrogen ChEBI	+	assimilation
[Ref.: #44005]	indole-3-acetic acid ChEBI	+	assimilation
[Ref.: #44005]	maltose ChEBI	+	assimilation
[Ref.: #44005]	methyl pyruvate ChEBI	+	assimilation
[Ref.: #44005]	propionate ChEBI	+	assimilation
[Ref.: #44005]	sucrose ChEBI	+	assimilation
[Ref.: #44005]	trehalose ChEBI	+	assimilation
[Ref.: #44005]	turanose ChEBI	+	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic resistance
[Ref.: #44005]	aztreonam ChEBI	yes
[Ref.: #44005]	rifamycin sv ChEBI	yes
[Ref.: #44005]	troleandomycin ChEBI	yes

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #44005]

Fatty acid	Percentage
C16:0 iso	33.5
C17:1v8c	25.8
C17:0	12.7
C15:0	7.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #44005]	Sample type/isolated from	Morella californica
[Ref.: #44005]	Sampling date	1986
[Ref.: #44005]	Geographic location (country and/or sea, region)	Westport-Legget, California
[Ref.: #44005]	Country	USA
[Ref.: #44005]	Country ISO 3 Code	USA
[Ref.: #44005]	Continent	North America

[Ref.: #64973]	Sample type/isolated from	Morella californica root nodule
[Ref.: #64973]	Geographic location (country and/or sea, region)	California, Westport-Legget
[Ref.: #64973]	Country	USA
[Ref.: #64973]	Country ISO 3 Code	USA
[Ref.: #64973]	Continent	North America
* marker position based on {}

Isolation sources categories

#Host

#Plants

#Shrub (Scrub)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KY744730 (>99% sequence identity) for Frankia from Microbeatlas

Aquatic Samples	69
Soil Samples	787
Animal Samples	34
Plant Samples	60
Total Samples	950

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64973]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64973]	Frankia sp. M16386 partial 16S rRNA gene, isolate M16386	AJ408869	1492	GenBank ENA	1834516 tax ID
[Ref.: #64973]	Pseudofrankia asymbiotica strain NRRL B-16386 16S ribosomal RNA gene, partial sequence	KY744730	1503	GenBank ENA	1834516 tax ID

[Ref.: #44005]	GC-content	72 mol%
[Ref.: #64973]	GC-content	72.0 mol%

Availability in culture collections External links

[Ref.: #64973]

Culture collection no.

DSM 100626, CECT 9040, NRRL B-16386

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Frankia saprophytica sp. nov., an atypical, non-infective (Nod-) and non-nitrogen fixing (Fix-) actinobacterium isolated from Coriaria nepalensis root nodules.	Nouioui I, Ghodhbane-Gtari F, Klenk HP, Gtari M	Int J Syst Evol Microbiol	10.1099/ijsem.0.002633	2018	*
Phylogeny	Frankia asymbiotica sp. nov., a non-infective actinobacterium isolated from Morella californica root nodule.	Nouioui I, Gueddou A, Ghodhbane-Gtari F, Rhode M, Gtari M, Klenk HP	Int J Syst Evol Microbiol	10.1099/ijsem.0.002153	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#44005

Imen Nouioui, Abdellatif Gueddou, Faten Ghodhbane-Gtari, Manfred Rhode, Maher Gtari, Hans-Peter Klenk: Frankia asymbiotica sp. nov., a non-infective actinobacterium isolated from Morella californica root nodule. IJSEM 67: 4897 - 4901 2017 ( DOI 10.1099/ijsem.0.002153 , PubMed 28918775 )

#64973

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100626

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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