Name: Petrothermobacter organivorans ANA
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 141042

Type strain:

Species: Petrothermobacter organivorans

Strain Designation: ANA

Culture col. no.: NBRC 112621, DSM 105015

Strain history: <- H. Tamaki, Natl. Inst. Advanced Industrial Sci. and Technol., Tsukuba, Japan; ANA <- D. Mayumi, S. Tamazawa, H. Tamaki

NCBI tax ID(s): 1856807 (species)

SI-ID 405979

DSM 105015

Section

Petrothermobacter organivorans ANA is an obligate anaerobe, Gram-negative, rod-shaped bacterium that was isolated from high temperature, deep subsurface oil, Yabase oil field, Akita Prefecture, Japan.

Gram-negative
rod-shaped
obligate anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Deferribacterota*
	Class	*Deferribacteres*
	Order	*Deferribacterales*
	Family	*Deferribacteraceae*
	Genus	*Petrothermobacter*
	Species	**Petrothermobacter organivorans**
	Full Scientific Name (LPSN)	Petrothermobacter organivorans Tamazawa et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43922]	Gram stain	negative

[Ref.: #43922]	Cell length	1.5-5.0 µm

[Ref.: #43922]	Cell width	0.6-0.7 µm

[Ref.: #43922]	Cell shape	rod-shaped

[Ref.: #43922]	Motility	no

[Ref.: #43922]

Colony color

brownish

Information on culture and growth conditions Culture and growth conditions

[Ref.: #64907]

Culture medium

DESULFOBACTERIUM MEDIUM (DSMZ Medium 383)

[Ref.: #64907]

Culture medium growth

[Ref.: #64907]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64907]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #43922]	growth	25-60 °C
[Ref.: #43922]	optimum	55 °C
[Ref.: #64907]	growth	55 °C

[Ref.: #43922]	Temperature range	thermophilic
[Ref.: #64907]	Temperature range	thermophilic

	pH	Kind of pH		pH
[Ref.: #43922]			optimum	7
[Ref.: #43922]			growth	6.0-8.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43922]

Oxygen tolerance

obligate anaerobe

[Ref.: #43922]

Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43922]		NaCl		growth	0-6	%(w/v)
[Ref.: #43922]		NaCl		optimum	2.5	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43922]	acetate ChEBI	+	carbon source
[Ref.: #43922]	acetate ChEBI	+	electron donor
[Ref.: #43922]	fumarate ChEBI	+	fermentation
[Ref.: #43922]	iron ChEBI	+	electron acceptor
[Ref.: #43922]	lactate ChEBI	+	carbon source
[Ref.: #43922]	lactate ChEBI	+	electron donor
[Ref.: #43922]	malate ChEBI	+	fermentation
[Ref.: #43922]	manganese ChEBI	+	electron acceptor
[Ref.: #43922]	nitrate ChEBI	+	electron acceptor
[Ref.: #43922]	peptone	+	fermentation
[Ref.: #43922]	pyruvate ChEBI	+	carbon source
[Ref.: #43922]	pyruvate ChEBI	+	electron donor
[Ref.: #43922]	pyruvate ChEBI	+	fermentation
[Ref.: #43922]	succinate ChEBI	-	electron donor
[Ref.: #43922]	succinate ChEBI	+	fermentation
[Ref.: #43922]	sulfate ChEBI	+	electron acceptor
[Ref.: #43922]	yeast extract	+	carbon source
[Ref.: #43922]	yeast extract	+	electron donor
[Ref.: #43922]	yeast extract	+	fermentation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43922]	ampicillin ChEBI				yes	100	µg/mL
[Ref.: #43922]	chloramphenicol ChEBI	yes	100	µg/mL
[Ref.: #43922]	gentamicin ChEBI	yes	100	µg/mL
[Ref.: #43922]	kanamycin ChEBI				yes	100	µg/mL
[Ref.: #43922]	rifampicin ChEBI				yes	100	µg/mL
[Ref.: #43922]	streptomycin ChEBI	yes	100	µg/mL
[Ref.: #43922]	tetracycline ChEBI				yes	100	µg/mL
[Ref.: #43922]	vancomycin ChEBI				yes	100	µg/mL

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43922]	Sample type/isolated from	high temperature, deep subsurface oil, Yabase oil field, Akita Prefecture, Japan
[Ref.: #43922]	Geographic location (country and/or sea, region)	Yabase oil fiel, Akita Prefecture
[Ref.: #43922]	Country	Japan
[Ref.: #43922]	Country ISO 3 Code	JPN
[Ref.: #43922]	Continent	Asia
[Ref.: #43922]	Geographic location	39.7000°/140.0830°

[Ref.: #64907]	Sample type/isolated from	high-temperature deep subsurface oil reservoir
[Ref.: #64907]	Geographic location (country and/or sea, region)	Akita Prefecture, Yabase oil field
[Ref.: #64907]	Country	Japan
[Ref.: #64907]	Country ISO 3 Code	JPN
[Ref.: #64907]	Continent	Asia
[Ref.: #64907]	Geographic location	39.7000°/140.0830°
* marker position based on {}

Isolation sources categories

#Condition	#Thermophilic (>45°C)	-
#Engineered	#Contamination	#Oil (Fuel)
#Environmental	#Terrestrial	-

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC155975 (>99% sequence identity) for Petrothermobacter organivorans subclade from Microbeatlas

Aquatic Samples	12
Soil Samples	1
Animal Samples
Plant Samples
Total Samples	13

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64907]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64907]	Petrothermobacter organivorans gene for 16S ribosomal RNA, partial sequence, strain: ANA	LC155975	1390	ENA	1856807 tax ID

[Ref.: #43922]	GC-content	34.4 mol%	high performance liquid chromatography (HPLC)
[Ref.: #64907]	GC-content	34.3 mol%	high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #64907]

Culture collection no.

NBRC 112621, DSM 105015

[Ref.: #96845]

SI-ID 405979

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Petrothermobacter organivorans gen. nov., sp. nov., a thermophilic, strictly anaerobic bacterium of the phylum Deferribacteres isolated from a deep subsurface oil reservoir.	Tamazawa S, Mayumi D, Mochimaru H, Sakata S, Maeda H, Wakayama T, Ikarashi M, Kamagata Y, Tamaki H	Int J Syst Evol Microbiol	10.1099/ijsem.0.002234	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43922

Satoshi Tamazawa, Daisuke Mayumi, Hanako Mochimaru, Susumu Sakata, Haruo Maeda, Tatsuki Wakayama, Masayuki Ikarashi, Yoichi Kamagata, Hideyuki Tamaki: Petrothermobacter organivorans gen. nov., sp. nov., a thermophilic, strictly anaerobic bacterium of the phylum Deferribacteres isolated from a deep subsurface oil reservoir. IJSEM 67: 3982 - 3986 2017 ( DOI 10.1099/ijsem.0.002234 , PubMed 28893364 )

#64907

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 105015

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96845

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID405979.1 )

* These data were automatically processed and therefore are not curated

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