Name: Paralkalibacillus indicireducens Bps-1
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 141039

Type strain:

Species: Paralkalibacillus indicireducens

Strain Designation: Bps-1

Culture col. no.: JCM 31808, NCIMB 15080

Strain history: T. Tamura and I. Yumoto; AIST, Japan; Bps-1.

NCBI tax ID(s): 1920268 (species)

Special Collections

Section

When using BacDive for research please cite our paper

Paralkalibacillus indicireducens Bps-1 is a facultative anaerobe, halophilic, spore-forming bacterium that forms circular colonies and was isolated from indigo fermentation liquor used for dyeing, produced from sukumo obtained from craft centre in Data City, Hokkaido, Japan.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
facultative anaerobe
halophilic
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Paralkalibacillus*
	Species	**Paralkalibacillus indicireducens**
	Full Scientific Name (LPSN)	Paralkalibacillus indicireducens Hirota et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43919]	Gram stain	positive

[Ref.: #43919]	Cell length	1.1-2.0 µm

[Ref.: #43919]	Cell width	0.5-0.8 µm

[Ref.: #43919]	Cell shape	rod-shaped

[Ref.: #43919]	Motility	yes

[Ref.: #43919]	Colony size	0.5-2.0 mm
[Ref.: #43919]	Colony color	creamy-white
[Ref.: #43919]	Colony shape	circular
[Ref.: #43919]	Incubation period	3 days
[Ref.: #43919]	Cultivation medium used	PYG-2 medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43919]

Culture medium

PYG-2 medium

[Ref.: #43919]

Culture medium growth

[Ref.: #43919]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #43919]	growth	18-40 °C
[Ref.: #43919]	optimum	33 °C
[Ref.: #67770]	growth	28 °C

	pH	Kind of pH		pH
[Ref.: #43919]			optimum	9-10
[Ref.: #43919]			growth	8-11

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43919]

Oxygen tolerance

facultative anaerobe

[Ref.: #43919]	Spore description	spherical endospores at the terminal position in the not swollen sporangia
[Ref.: #43919]	Type of spore	endospore
[Ref.: #43919]	Ability of spore formation	yes

[Ref.: #43919]

Halophily / tolerance level

halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43919]		NaCl		growth	0-10	%(w/v)
[Ref.: #43919]		NaCl		optimum	7	%(w/v)

[Ref.: #43919]	Observation	quinone MK-7
[Ref.: #67770]	Observation	quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43919]	2-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #43919]	5-dehydro-D-gluconate ChEBI	+	builds acid from
[Ref.: #43919]	amygdalin ChEBI	-	builds acid from
[Ref.: #43919]	arbutin ChEBI	-	builds acid from
[Ref.: #43919]	casein	-	hydrolysis
[Ref.: #43919]	cellobiose ChEBI	-	builds acid from
[Ref.: #43919]	cellulose ChEBI	-	hydrolysis
[Ref.: #43919]	D-arabinose ChEBI	-	builds acid from
[Ref.: #43919]	D-arabitol ChEBI	-	builds acid from
[Ref.: #43919]	D-fructose ChEBI	+	builds acid from
[Ref.: #43919]	D-fucose ChEBI	-	builds acid from
[Ref.: #43919]	D-galactose ChEBI	-	builds acid from
[Ref.: #43919]	D-glucose ChEBI	-	builds acid from
[Ref.: #43919]	D-lyxose ChEBI	+	builds acid from
[Ref.: #43919]	D-mannose ChEBI	-	builds acid from
[Ref.: #43919]	D-ribose ChEBI	+	builds acid from
[Ref.: #43919]	D-tagatose ChEBI	-	builds acid from
[Ref.: #43919]	D-tagatose ChEBI	+	builds acid from
[Ref.: #43919]	D-xylose ChEBI	-	builds acid from
[Ref.: #43919]	erythritol ChEBI	-	builds acid from
[Ref.: #43919]	galactitol ChEBI	-	builds acid from
[Ref.: #43919]	gelatin ChEBI	-	hydrolysis
[Ref.: #43919]	gentiobiose ChEBI	-	builds acid from
[Ref.: #43919]	gluconate ChEBI	-	builds acid from
[Ref.: #43919]	inulin ChEBI	-	builds acid from
[Ref.: #43919]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43919]	L-arabitol ChEBI	-	builds acid from
[Ref.: #43919]	L-sorbose ChEBI	-	builds acid from
[Ref.: #43919]	L-xylose ChEBI	-	builds acid from
[Ref.: #43919]	lactose ChEBI	-	builds acid from
[Ref.: #43919]	maltose ChEBI	-	builds acid from
[Ref.: #43919]	mannitol ChEBI	-	builds acid from
[Ref.: #43919]	melibiose ChEBI	+	builds acid from
[Ref.: #43919]	methyl alpha-D-glucopyranoside ChEBI	-	builds acid from
[Ref.: #43919]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from
[Ref.: #43919]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43919]	nitrate ChEBI	-	reduction
[Ref.: #43919]	raffinose ChEBI	+	builds acid from
[Ref.: #43919]	rhamnose ChEBI	-	builds acid from
[Ref.: #43919]	sorbitol ChEBI	-	builds acid from
[Ref.: #43919]	starch ChEBI	-	builds acid from
[Ref.: #43919]	starch ChEBI	-	hydrolysis
[Ref.: #43919]	sucrose ChEBI	+	builds acid from
[Ref.: #43919]	trehalose ChEBI	-	builds acid from
[Ref.: #43919]	turanose ChEBI	+	builds acid from
[Ref.: #43919]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43919]	tween 40 ChEBI	-	hydrolysis
[Ref.: #43919]	tween 60 ChEBI	-	hydrolysis
[Ref.: #43919]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43919]	xylan ChEBI	-	hydrolysis
[Ref.: #43919]	xylitol ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43919]	6-phospho-beta-galactosidase	+	3.2.1.85
[Ref.: #43919]	acid phosphatase	+	3.1.3.2
[Ref.: #43919]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43919]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43919]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43919]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43919]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43919]	beta-glucosidase	-	3.2.1.21
[Ref.: #43919]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43919]	catalase	+	1.11.1.6
[Ref.: #43919]	chymotrypsin	+	3.4.4.5
[Ref.: #43919]	cystine arylamidase	-	3.4.11.3
[Ref.: #43919]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43919]	esterase (C 4)	+
[Ref.: #43919]	esterase Lipase (C 8)	+
[Ref.: #43919]	leucine arylamidase	+	3.4.11.1
[Ref.: #43919]	lipase (C 14)	-
[Ref.: #43919]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43919]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43919]	trypsin	-	3.4.21.4
[Ref.: #43919]	urease	+	3.5.1.5
[Ref.: #43919]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43919]	Sample type/isolated from	indigo fermentation liquor used for dyeing, produced from sukumo (composted Polygonum indigo leaves) obtained from craft centre in Data City, Hokkaido, Japan
[Ref.: #43919]	Geographic location (country and/or sea, region)	Data City, Hokkaido
[Ref.: #43919]	Country	Japan
[Ref.: #43919]	Country ISO 3 Code	JPN
[Ref.: #43919]	Continent	Asia
[Ref.: #43919]	Geographic location	42.4708°/140.8750°
[Ref.: #43919]	Enrichment culture	sukumo/extract medium
[Ref.: #43919]	Enrichment culture composition	15 ml sukumo hydrolysate, 2 g indigo carmine, 15 g gellan gum in 885 ml distilled water, pH 10
[Ref.: #43919]	Enrichment culture duration	2 days
[Ref.: #43919]	Enrichment culture temperature	27 ̊C

[Ref.: #67770]	Sample type/isolated from	Indigo fermentation liquor from a craft centre in Data City
[Ref.: #67770]	Geographic location (country and/or sea, region)	Hokkaido
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Biodegradation	#Composting
#Environmental	#Microbial community	-
#Host	#Plants	#Herbaceous plants (Grass,Crops)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LC197841 (>99% sequence identity) for Paralkalibacillus indicireducens subclade from Microbeatlas

Aquatic Samples	28
Soil Samples	78
Animal Samples	53
Plant Samples	28
Total Samples	187

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43919]	Paralkalibacillus indicireducens gene for 16S ribosomal RNA, partial sequence, strain: M3-6	LC197841		GenBank ENA

[Ref.: #67770]

GC-content

40.3 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #43919]

Culture collection no.

JCM 31808, NCIMB 15080

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paralkalibacillus indicireducens gen., nov., sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor used for dyeing.	Hirota K, Nishita M, Matsuyama H, Yumoto I	Int J Syst Evol Microbiol	10.1099/ijsem.0.002248	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43919

Kikue Hirota, Masatoshi Nishita, Hidetoshi Matsuyama, Isao Yumoto: Paralkalibacillus indicireducens gen., nov., sp. nov., an indigo-reducing obligate alkaliphile isolated from indigo fermentation liquor used for dyeing. IJSEM 67: 4050 - 4056 2017 ( DOI 10.1099/ijsem.0.002248 , PubMed 28905696 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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