Name: Halobacterium litoreum ZS-54-S2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 141038

Type strain:

Species: Halobacterium litoreum

Strain Designation: ZS-54-S2

Culture col. no.: CGMCC 1.12562, JCM 30038

Strain history: H.-L. Cui ZS-54-S2.

NCBI tax ID(s): 2039234 (species)

Section

Halobacterium litoreum ZS-54-S2 is a mesophilic, Gram-negative, motile archaeon that forms circular colonies and was isolated from sediment of Zhoushan marine solar saltern, Zhejiang Province, China.

colony-forming
Gram-negative
motile
rod-shaped
mesophilic
16S sequence
Archaea
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Archaea"
	Phylum	*Methanobacteriota*
	Class	*Halobacteria*
	Order	*Halobacteriales*
	Family	*Halobacteriaceae*
	Genus	*Halobacterium*
	Species	**Halobacterium litoreum**
	Full Scientific Name (LPSN)	Halobacterium litoreum Lü et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43918]	Gram stain	negative

[Ref.: #43918]	Cell length	0.8-2.0 µm

[Ref.: #43918]	Cell width	0.4-0.5 µm

[Ref.: #43918]	Cell shape	rod-shaped

[Ref.: #43918]	Motility	yes

[Ref.: #43918]	Colony color	red
[Ref.: #43918]	Colony shape	circular
[Ref.: #43918]	Cultivation medium used	Neutral haloarchaeal medium

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43918]

Culture medium

Neutral haloarchaeal medium

[Ref.: #43918]

Culture medium growth

[Ref.: #43918]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #43918]	optimum	35 °C
[Ref.: #43918]	growth	20-50 °C
[Ref.: #67770]	growth	37 °C

[Ref.: #43918]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43918]			optimum	7
[Ref.: #43918]			growth	5.0-9.5

Information on physiology and metabolism Physiology and metabolism

	Salt		Salt conc.
[Ref.: #43918]	NaCl	optimum	3.9	M
[Ref.: #43918]	NaCl	growth	1.7-4.8	M
[Ref.: #43918]	MgCl2	optimum	0.05	M
[Ref.: #43918]	MgCl2	growth	0.005-1	M

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43918]	acetate ChEBI	+	carbon source
[Ref.: #43918]	casein	-	hydrolysis
[Ref.: #43918]	citrate ChEBI	-	carbon source
[Ref.: #43918]	D-fructose ChEBI	-	carbon source
[Ref.: #43918]	D-galactose ChEBI	-	carbon source
[Ref.: #43918]	D-glucose ChEBI	-	carbon source
[Ref.: #43918]	D-mannitol ChEBI	-	carbon source
[Ref.: #43918]	D-mannose ChEBI	-	carbon source
[Ref.: #43918]	D-ribose ChEBI	-	carbon source
[Ref.: #43918]	D-sorbitol ChEBI	-	carbon source
[Ref.: #43918]	D-xylose ChEBI	-	carbon source
[Ref.: #43918]	fumarate ChEBI	+	carbon source
[Ref.: #43918]	gelatin ChEBI	+	hydrolysis
[Ref.: #43918]	glycerol ChEBI	+	carbon source
[Ref.: #43918]	glycine ChEBI	-	carbon source
[Ref.: #43918]	L-alanine ChEBI	-	carbon source
[Ref.: #43918]	L-arginine ChEBI	-	carbon source
[Ref.: #43918]	L-aspartate ChEBI	-	carbon source
[Ref.: #43918]	L-glutamate ChEBI	-	carbon source
[Ref.: #43918]	L-lysine ChEBI	-	carbon source
[Ref.: #43918]	L-malate ChEBI	-	carbon source
[Ref.: #43918]	L-ornithine ChEBI	-	carbon source
[Ref.: #43918]	L-sorbose ChEBI	-	carbon source
[Ref.: #43918]	lactate ChEBI	-	carbon source
[Ref.: #43918]	lactose ChEBI	-	carbon source
[Ref.: #43918]	maltose ChEBI	-	carbon source
[Ref.: #43918]	nitrate ChEBI	+	builds gas from
[Ref.: #43918]	nitrate ChEBI	+	reduction
[Ref.: #43918]	pyruvate ChEBI	+	carbon source
[Ref.: #43918]	starch ChEBI	-	carbon source
[Ref.: #43918]	starch ChEBI	-	hydrolysis
[Ref.: #43918]	succinate ChEBI	-	carbon source
[Ref.: #43918]	sucrose ChEBI	+	carbon source
[Ref.: #43918]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43918]	ampicillin ChEBI				yes	10	µg (disc)
[Ref.: #43918]	bacitracin ChEBI	yes	0.04	Unit (disc)
[Ref.: #43918]	chloramphenicol ChEBI				yes	30	µg (disc)
[Ref.: #43918]	ciprofloxacin ChEBI				yes	5	µg (disc)
[Ref.: #43918]	erythromycin ChEBI				yes	15	µg (disc)
[Ref.: #43918]	gentamicin ChEBI				yes	10	µg (disc)
[Ref.: #43918]	kanamycin ChEBI				yes	30	µg (disc)
[Ref.: #43918]	nalidixic acid ChEBI				yes	30	µg (disc)
[Ref.: #43918]	neomycin ChEBI				yes	30	µg (disc)
[Ref.: #43918]	nitrofurantoin ChEBI				yes	300	µg (disc)
[Ref.: #43918]	norfloxacin ChEBI				yes	10	µg (disc)
[Ref.: #43918]	novobiocin ChEBI	yes	30	µg (disc)
[Ref.: #43918]	nystatin ChEBI	yes	100	µg (disc)
[Ref.: #43918]	penicillin g ChEBI				yes	10	Unit (disc)
[Ref.: #43918]	rifampicin ChEBI				yes	5	µg (disc)
[Ref.: #43918]	streptomycin ChEBI				yes	10	µg (disc)
[Ref.: #43918]	tetracycline ChEBI				yes	30	µg (disc)
[Ref.: #43918]	trimethoprim ChEBI				yes	5	µg (disc)
[Ref.: #43918]	vancomycin ChEBI				yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43918]		hydrogen sulfide ChEBI	yes
[Ref.: #43918]		indole ChEBI	yes

	Enzymes	Enzyme	Enzyme activity	EC number
[Ref.: #43918]		catalase	+	1.11.1.6
[Ref.: #43918]		cytochrome oxidase	+	1.9.3.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43918]	Sample type/isolated from	sediment of Zhoushan marine solar saltern, Zhejiang Province, China
[Ref.: #43918]	Geographic location (country and/or sea, region)	Zhejiang Province
[Ref.: #43918]	Country	China
[Ref.: #43918]	Country ISO 3 Code	CHN
[Ref.: #43918]	Continent	Asia
[Ref.: #43918]	Enrichment culture	Neutral haloarchaeal medium
[Ref.: #43918]	Enrichment culture composition	yeast extract 0.05 g/L, fish peptone 0.25 g/L, sodium pyruvate 1.0 g/L, KCl 5.4 g/L, K2HPO4 0.3 g/L, CaCl2 0.29 g/L, NH4Cl 0.27 g/L, MgSO4.7H2O 26.8 g/L, MgCl2.6H2O 23.0 g/L, NaCl 184.0 g/L, pH 7.0–7.2
[Ref.: #43918]	Enrichment culture duration	4 weeks
[Ref.: #43918]	Enrichment culture temperature	37 ̊C

[Ref.: #67770]	Sample type/isolated from	Sediment of Zhoushan marine solar saltern
[Ref.: #67770]	Geographic location (country and/or sea, region)	Zhejiang Province
[Ref.: #67770]	Country	China
[Ref.: #67770]	Country ISO 3 Code	CHN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Condition	#Saline	-
#Environmental	#Aquatic	#Marine
#Environmental	#Terrestrial	#Sediment

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KC918831 (>99% sequence identity) for Halobacterium from Microbeatlas

Aquatic Samples	60
Soil Samples	4
Animal Samples	2
Plant Samples
Total Samples	66

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43918]	16S rRNA gene sequence	KC918831		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Halobacterium litoreum ZS-54-S2	GCA_021233415	complete	GenBank	2039234 tax ID	*

[Ref.: #67770]

GC-content

66.7 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Halobacterium litoreum ZS-54-S2 NCBI: GCA_021233415.1
[Ref.: #69480]	flagellated	no	95.701	no
[Ref.: #69480]	gram-positive	no	96.441	yes
[Ref.: #69480]	anaerobic	no	96.271	no
[Ref.: #69480]	aerobic	yes	86.143	no
[Ref.: #69480]	halophile	yes	90.978	no
[Ref.: #69480]	spore-forming	no	95.11	no
[Ref.: #69480]	motile	no	61.008	yes
[Ref.: #69480]	thermophile	no	86.92	yes
[Ref.: #69480]	glucose-util	yes	86.126	no
[Ref.: #69480]	glucose-ferment	no	87.554	no

Availability in culture collections External links

[Ref.: #43918]

Culture collection no.

CGMCC 1.12562, JCM 30038

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Halobacterium litoreum sp. nov., isolated from a marine solar saltern.	Lu ZZ, Li Y, Zhou Y, Cui HL, Li ZR	Int J Syst Evol Microbiol	10.1099/ijsem.0.002259	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43918

Zhen-Zhen Lü, Yang Li, Yao Zhou, Heng-Lin Cui, Zheng-Rong Li: Halobacterium litoreum sp. nov., isolated from a marine solar saltern. IJSEM 67: 4095 - 4099 2017 ( DOI 10.1099/ijsem.0.002259 , PubMed 28901903 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

* These data were automatically processed and therefore are not curated

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