Name: Angustibacter speluncae YC2-20
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 141003

Type strain:

Species: Angustibacter speluncae

Strain Designation: YC2-20

Culture col. no.: KCTC 39842, DSM 103769, JCM 32752

Strain history: <- Soon Dong Lee, Jeju National Univ.

NCBI tax ID(s): 1940617 (species)

SI-ID 407208

DSM 103769

Special Collections

Other strains from Angustibacter speluncae

Section

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Angustibacter speluncae YC2-20 is an aerobe, Gram-positive, motile bacterium that forms circular colonies and was isolated from pieces of stalactites collected at Yongcheon Cave in Jeju, Republic of Korea.

coccus-shaped
colony-forming
Gram-positive
motile
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Kineosporiales*
	Family	*Kineosporiaceae*
	Genus	*Angustibacter*
	Species	**Angustibacter speluncae**
	Full Scientific Name (LPSN)	Angustibacter speluncae Ko and Lee 2017

Information on morphological and physiological properties Morphology

[Ref.: #43893]	Gram stain	positive

[Ref.: #43893]	Cell length	0.54-0.83 µm
[Ref.: #43893]	Cell length	1.3-1.6 µm

[Ref.: #43893]	Cell width	0.54-0.83 µm
[Ref.: #43893]	Cell width	0.7 µm

[Ref.: #43893]	Cell shape	coccus-shaped
[Ref.: #43893]	Cell shape	rod-shaped

[Ref.: #43893]	Motility	yes

[Ref.: #43893]	Colony size	0.5-1.2 mm
[Ref.: #43893]	Colony color	orange
[Ref.: #43893]	Colony shape	circular
[Ref.: #43893]	Incubation period	7 days
[Ref.: #43893]	Cultivation medium used	Trypticase soy agar

Multimedia content

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Caption:

Medium 535 28°C

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Intellectual property rights:

Information on culture and growth conditions Culture and growth conditions

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Culture medium

ISP 2

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

Trypticase soy agar

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Culture medium growth

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Culture medium

TRYPTICASE SOY BROTH AGAR (DSMZ Medium 535)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

R2A MEDIUM (DSMZ Medium 830)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #43893]	growth	20-37 °C
[Ref.: #43893]	optimum	30 °C
[Ref.: #64876]	growth	28 °C
[Ref.: #67770]	growth	28 °C
[Ref.: #67771]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #43893]			optimum	6.0-7.0
[Ref.: #43893]			growth	6.0-10.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43893]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43893]		NaCl		optimum	0	%(w/v)
[Ref.: #43893]		NaCl	no	growth	3	%(w/v)

[Ref.: #43893]	Observation	quinone MK-9
[Ref.: #43893]	Observation	Mycolic acids
[Ref.: #67770]	Observation	quinones: MK-9(H₄)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43893]	2-dehydro-D-gluconate ChEBI	-	builds acid from
[Ref.: #43893]	5-dehydro-D-gluconate ChEBI	+/-	builds acid from
[Ref.: #43893]	adipate ChEBI	-	assimilation
[Ref.: #43893]	amygdalin ChEBI	-	builds acid from
[Ref.: #43893]	arbutin ChEBI	-	builds acid from
[Ref.: #43893]	cellobiose ChEBI	+/-	builds acid from
[Ref.: #43893]	citrate ChEBI	-	assimilation
[Ref.: #43893]	D-arabinose ChEBI	+	builds acid from
[Ref.: #43893]	D-arabinose ChEBI	+/-	assimilation
[Ref.: #43893]	D-arabitol ChEBI	+/-	builds acid from
[Ref.: #43893]	D-fructose ChEBI	+	builds acid from
[Ref.: #43893]	D-fucose ChEBI	-	builds acid from
[Ref.: #43893]	D-galactose ChEBI	+	builds acid from
[Ref.: #43893]	D-glucose ChEBI	+	assimilation
[Ref.: #43893]	D-glucose ChEBI	+	builds acid from
[Ref.: #43893]	D-lyxose ChEBI	+/-	builds acid from
[Ref.: #43893]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43893]	D-mannitol ChEBI	+	assimilation
[Ref.: #43893]	D-mannose ChEBI	-	assimilation
[Ref.: #43893]	D-mannose ChEBI	+	builds acid from
[Ref.: #43893]	D-ribose ChEBI	-	builds acid from
[Ref.: #43893]	D-sorbitol ChEBI	+/-	builds acid from
[Ref.: #43893]	D-tagatose ChEBI	-	builds acid from
[Ref.: #43893]	D-xylose ChEBI	+	builds acid from
[Ref.: #43893]	decanoate ChEBI	-	assimilation
[Ref.: #43893]	erythritol ChEBI	-	builds acid from
[Ref.: #43893]	esculin ChEBI	+	degradation
[Ref.: #43893]	esculin ChEBI	+/-	builds acid from
[Ref.: #43893]	galactitol ChEBI	-	builds acid from
[Ref.: #43893]	gelatin ChEBI	+	hydrolysis
[Ref.: #43893]	gentiobiose ChEBI	+/-	builds acid from
[Ref.: #43893]	gluconate ChEBI	-	builds acid from
[Ref.: #43893]	gluconate ChEBI	+	assimilation
[Ref.: #43893]	glucose ChEBI	-	fermentation
[Ref.: #43893]	glycerol ChEBI	-	builds acid from
[Ref.: #43893]	glycogen ChEBI	+/-	builds acid from
[Ref.: #43893]	inulin ChEBI	-	builds acid from
[Ref.: #43893]	L-arabinose ChEBI	+	builds acid from
[Ref.: #43893]	L-arabitol ChEBI	-	builds acid from
[Ref.: #43893]	L-fucose ChEBI	-	builds acid from
[Ref.: #43893]	L-sorbose ChEBI	+	builds acid from
[Ref.: #43893]	L-xylose ChEBI	-	builds acid from
[Ref.: #43893]	lactose ChEBI	+/-	builds acid from
[Ref.: #43893]	malate ChEBI	+/-	assimilation
[Ref.: #43893]	maltose ChEBI	+	assimilation
[Ref.: #43893]	maltose hydrate	+/-	builds acid from
[Ref.: #43893]	melezitose ChEBI	+/-	builds acid from
[Ref.: #43893]	melibiose ChEBI	+/-	builds acid from
[Ref.: #43893]	methyl alpha-D-glucopyranoside ChEBI	+/-	builds acid from
[Ref.: #43893]	methyl alpha-D-mannoside ChEBI	-	builds acid from
[Ref.: #43893]	methyl beta-D-xylopyranoside ChEBI	-	builds acid from
[Ref.: #43893]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43893]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #43893]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #43893]	nitrate ChEBI	-	reduction
[Ref.: #43893]	phenylacetate ChEBI	-	assimilation
[Ref.: #43893]	raffinose ChEBI	+/-	builds acid from
[Ref.: #43893]	rhamnose ChEBI	-	builds acid from
[Ref.: #43893]	ribitol ChEBI	-	builds acid from
[Ref.: #43893]	salicin ChEBI	+/-	builds acid from
[Ref.: #43893]	starch ChEBI	+/-	builds acid from
[Ref.: #43893]	sucrose ChEBI	+/-	builds acid from
[Ref.: #43893]	trehalose ChEBI	+/-	builds acid from
[Ref.: #43893]	turanose ChEBI	+/-	builds acid from
[Ref.: #43893]	xylitol ChEBI	+/-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43893]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43893]	acid phosphatase	-	3.1.3.2
[Ref.: #43893]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43893]	alpha-chymotrypsin	+/-	3.4.21.1
[Ref.: #43893]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43893]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43893]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43893]	alpha-mannosidase	+/-	3.2.1.24
[Ref.: #43893]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43893]	beta-galactosidase	+	3.2.1.23
[Ref.: #43893]	beta-glucosidase	+	3.2.1.21
[Ref.: #43893]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43893]	catalase	+	1.11.1.6
[Ref.: #43893]	cystine arylamidase	-	3.4.11.3
[Ref.: #43893]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43893]	esterase (C 4)	+
[Ref.: #43893]	esterase Lipase (C 8)	+
[Ref.: #43893]	leucine arylamidase	+	3.4.11.1
[Ref.: #43893]	lipase (C 14)	-
[Ref.: #43893]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43893]	naphthol-AS-BI-phosphohydrolase	+/-
[Ref.: #43893]	trypsin	+/-	3.4.21.4
[Ref.: #43893]	urease	-	3.5.1.5
[Ref.: #43893]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43893]

Fatty acid	Percentage
C18:1ω9c	20.1
C16:0	18.9
C16:1 iso H	11.9
C16:0 iso	11.1
C18:0	8.9
C12:0	3.9
C15:0 iso	3.4
C17:0	3.1
C15:0 anteiso	2.9
C16:1ω7c / C16:1ω6c	2.4
C17:0 anteiso	1.8
C17:1ω8c	1.6
C15:1ω6c	1.6
C14:0 iso	1.4
C17:0 iso	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43893]	Sample type/isolated from	pieces of stalactites collected at Yongcheon Cave in Jeju, Republic of Korea
[Ref.: #43893]	Geographic location (country and/or sea, region)	Yongcheon Cave, Jeju
[Ref.: #43893]	Country	Republic of Korea
[Ref.: #43893]	Country ISO 3 Code	KOR
[Ref.: #43893]	Continent	Asia
[Ref.: #43893]	Enrichment culture	Starch.-casein agar
[Ref.: #43893]	Enrichment culture duration	4 weeks
[Ref.: #43893]	Enrichment culture temperature	30 ̊C

[Ref.: #64876]	Sample type/isolated from	pieces of stalactites from a lava cave
[Ref.: #64876]	Geographic location (country and/or sea, region)	Jeju, Yongcheon Cave
[Ref.: #64876]	Country	Republic of Korea
[Ref.: #64876]	Country ISO 3 Code	KOR
[Ref.: #64876]	Continent	Asia
[Ref.: #64876]	Geographic location	33.5617°/126.8480°

[Ref.: #67770]	Sample type/isolated from	Pieces of stalactites from the Yongcheon Cave in Jeju
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From pieces of stalactites collected at the Yongcheon Cave in Jeju
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Geologic
#Environmental	#Terrestrial	#Volcanic

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence LT719160 (>99% sequence identity) for Angustibacter speluncae subclade from Microbeatlas

Aquatic Samples	416
Soil Samples	619
Animal Samples	789
Plant Samples	364
Total Samples	2188

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64876]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64876]	Angustibacter sp. YC2-20 partial 16S rRNA gene, strain YC2-20	LT719160	1421	GenBank ENA	1940617 tax ID

[Ref.: #43893]	GC-content	73.1 mol%	high performance liquid chromatography (HPLC)
[Ref.: #64876]	GC-content	73.1 mol%

Availability in culture collections External links

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Culture collection no.

KCTC 39842, DSM 103769, JCM 32752

[Ref.: #96826]

SI-ID 407208

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Angustibacter speluncae sp. nov., isolated from a lava cave stalactite.	Ko DH, Lee SD	Int J Syst Evol Microbiol	10.1099/ijsem.0.002108	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43893

Dong Hyo Ko, Soon Dong Lee: Angustibacter speluncae sp. nov., isolated from a lava cave stalactite. IJSEM 67: 3283 - 3288 2017 ( DOI 10.1099/ijsem.0.002108 , PubMed 28857735 )

#64876

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103769

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96826

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID407208.1 )

* These data were automatically processed and therefore are not curated

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