Name: Novosphingobium fontis STM-14
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140948

Type strain:

Species: Novosphingobium fontis

Strain Designation: STM-14

Culture col. no.: LMG 29290, KCTC 42983, BCRC 80924

Strain history: <- Wen-Ming Chen, National Kaohsiung Marine Univ.

NCBI tax ID(s): 1737443 (species)

Section

Novosphingobium fontis STM-14 is an aerobe, chemoheterotroph, Gram-negative bacterium that forms circular colonies and was isolated from spring in the vicinity of Miaoli County, Taiwan.

colony-forming
Gram-negative
rod-shaped
aerobe
chemoheterotroph
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Sphingomonadales*
	Family	*Erythrobacteraceae*
	Genus	*Novosphingobium*
	Species	**Novosphingobium fontis**
	Full Scientific Name (LPSN)	Novosphingobium fontis Sheu et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43841]	Gram stain	negative

[Ref.: #43841]	Cell length	1.0-3.0 µm

[Ref.: #43841]	Cell width	0.5-0.8 µm

[Ref.: #43841]	Cell shape	rod-shaped

[Ref.: #43841]	Motility	no

[Ref.: #43841]	Colony size	0.4-0.6 mm
[Ref.: #43841]	Colony color	cream
[Ref.: #43841]	Colony shape	circular
[Ref.: #43841]	Incubation period	2 days
[Ref.: #43841]	Cultivation medium used	R2A agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

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Culture medium

LB (Luria-Bertani) MEDIUM

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Culture medium growth

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Culture medium

Tripticase soy agar

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Culture medium growth

		Temperature
[Ref.: #43841]	growth	15-37 °C
[Ref.: #43841]	optimum	30 °C
[Ref.: #67771]	growth	25 °C

[Ref.: #43841]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43841]			optimum	6
[Ref.: #43841]			growth	5.0-9.0

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43841]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

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Nutrition type

chemoheterotroph

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43841]		NaCl		growth	0-0.5	%(w/v)
[Ref.: #43841]		NaCl		optimum	0	%(w/v)

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Observation

Quinone Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43841]	adipate ChEBI	-	growth
[Ref.: #43841]	beta-hydroxybutyrate ChEBI	+	growth
[Ref.: #43841]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #43841]	casein	-	hydrolysis
[Ref.: #43841]	cellobiose ChEBI	-	growth
[Ref.: #43841]	chitin ChEBI	-	hydrolysis
[Ref.: #43841]	citrate ChEBI	+	growth
[Ref.: #43841]	corn oil	+	hydrolysis
[Ref.: #43841]	D-fructose ChEBI	-	growth
[Ref.: #43841]	D-galactose ChEBI	-	growth
[Ref.: #43841]	D-glucose ChEBI	-	growth
[Ref.: #43841]	D-mannitol ChEBI	-	growth
[Ref.: #43841]	D-mannose ChEBI	-	growth
[Ref.: #43841]	D-rhamnose ChEBI	-	growth
[Ref.: #43841]	decanoate ChEBI	-	growth
[Ref.: #43841]	dna ChEBI	+	hydrolysis
[Ref.: #43841]	esculin ChEBI	+	hydrolysis
[Ref.: #43841]	gelatin ChEBI	-	hydrolysis
[Ref.: #43841]	gluconate ChEBI	-	growth
[Ref.: #43841]	glucose ChEBI	-	fermentation
[Ref.: #43841]	L-alanine 4-nitroanilide	+	growth
[Ref.: #43841]	L-arabinose ChEBI	+	growth
[Ref.: #43841]	L-histidine ChEBI	-	growth
[Ref.: #43841]	L-proline ChEBI	+	growth
[Ref.: #43841]	L-serine ChEBI	+	growth
[Ref.: #43841]	malate ChEBI	+	growth
[Ref.: #43841]	maltose ChEBI	-	growth
[Ref.: #43841]	N-acetylglucosamine ChEBI	-	growth
[Ref.: #43841]	nitrate ChEBI	+	reduction
[Ref.: #43841]	starch ChEBI	-	hydrolysis
[Ref.: #43841]	sucrose ChEBI	-	growth
[Ref.: #43841]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43841]	tween 40 ChEBI	+	hydrolysis
[Ref.: #43841]	tween 60 ChEBI	+	hydrolysis
[Ref.: #43841]	tween 80 ChEBI	-	hydrolysis
[Ref.: #43841]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43841]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43841]	acid phosphatase	+	3.1.3.2
[Ref.: #43841]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43841]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43841]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43841]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43841]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43841]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43841]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43841]	beta-galactosidase	-	3.2.1.23
[Ref.: #43841]	beta-glucosidase	+	3.2.1.21
[Ref.: #43841]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43841]	catalase	-	1.11.1.6
[Ref.: #43841]	cystine arylamidase	+	3.4.11.3
[Ref.: #43841]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43841]	esterase (C 4)	+
[Ref.: #43841]	esterase Lipase (C 8)	+
[Ref.: #43841]	leucine arylamidase	+	3.4.11.1
[Ref.: #43841]	lipase (C 14)	+
[Ref.: #43841]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #43841]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43841]	trypsin	+	3.4.21.4
[Ref.: #43841]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43841]

Fatty acid	Percentage
C17:1ω6c	20
C16:1ω7c / C16:1ω6c	18.4
C18:1ω7c	14.5
C15:0 2OH	10.6
C17:1ω8c	10
C14:0 2OH	7.2
C16:0 2OH	4.6
C15:1ω6c	2.7
11-methyl C18:1ω7c	1.7
C13:0 2OH	1.3
C17:0	0.9
C16:0 2OH	0.9
C18:0	0.6
C14:0	0.6
C18:1ω9c	0.6
C16:1ω5c	0.4

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43841]	Sample type/isolated from	spring in the vicinity of Miaoli County, Taiwan
[Ref.: #43841]	Geographic location (country and/or sea, region)	Miaoli County
[Ref.: #43841]	Country	Taiwan
[Ref.: #43841]	Country ISO 3 Code	TWN
[Ref.: #43841]	Continent	Asia
[Ref.: #43841]	Geographic location	24.5483°/120.9260°
[Ref.: #43841]	Enrichment culture	R2A agar
[Ref.: #43841]	Enrichment culture temperature	25 ̊C

[Ref.: #67771]	Sample type/isolated from	From mountain spring water
[Ref.: #67771]	Geographic location (country and/or sea, region)	Miaoli country
[Ref.: #67771]	Country	Taiwan
[Ref.: #67771]	Country ISO 3 Code	TWN
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Spring

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43841]	16S rRNA gene sequence	LN890293		GenBank

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GC-content

66.6 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #43841]

Culture collection no.

LMG 29290, KCTC 42983, BCRC 80924

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Novosphingobium aureum sp. nov., a marine bacterium isolated from salt flat sediment.	Yoo Y, Kim D, Lee H, Khim JS, Kim B, Yang D, Pathiraja D, Choi IG, Kim JJ	Int J Syst Evol Microbiol	10.1099/ijsem.0.004930	2021	*
Phylogeny	Novosphingobium fontis sp. nov., isolated from a spring.	Sheu SY, Liu LP, Young CC, Chen WM	Int J Syst Evol Microbiol	10.1099/ijsem.0.001973	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43841

Shih-Yi Sheu, Li-Ping Liu, Chiu-Chung Young, Wen-Ming Chen: Novosphingobium fontis sp. nov., isolated from a spring. IJSEM 67: 2423 - 2429 2017 ( DOI 10.1099/ijsem.0.001973 , PubMed 28741990 )

#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;
* These data were automatically processed and therefore are not curated

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