Name: Brachybacterium horti THG-S15-4
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140947

Type strain:

Species: Brachybacterium horti

Strain Designation: THG-S15-4

Culture col. no.: KCTC 39563, CCTCC AB 2015116, JCM 31742

Strain history: CCTCC AB 2015116 <-- T.-H. Yi; Kyung Hee Univ., South Korea; THG-S15-4.

NCBI tax ID(s): 1517578 (species)

Section

Brachybacterium horti THG-S15-4 is a facultative anaerobe, Gram-positive, coccus-shaped bacterium that forms circular colonies and was isolated from Soil from the Guro-gu district.

coccus-shaped
colony-forming
Gram-positive
facultative anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Micrococcales*
	Family	*Dermabacteraceae*
	Genus	*Brachybacterium*
	Species	**Brachybacterium horti**
	Full Scientific Name (LPSN)	Brachybacterium horti Singh et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43840]	Gram stain	positive

[Ref.: #43840]	Cell length	0.5-0.8 µm

[Ref.: #43840]	Cell shape	coccus-shaped

[Ref.: #43840]	Motility	no

[Ref.: #43840]	Colony size	2-4 mm
[Ref.: #43840]	Colony color	White
[Ref.: #43840]	Colony shape	circular
[Ref.: #43840]	Incubation period	2 days
[Ref.: #43840]	Cultivation medium used	Nutrient agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43840]

Culture medium

LB (Luria-Bertani) MEDIUM

[Ref.: #43840]

Culture medium growth

[Ref.: #43840]

Culture medium

Marine agar (MA)

[Ref.: #43840]

Culture medium growth

[Ref.: #43840]

Culture medium

Nutrient agar (NA)

[Ref.: #43840]

Culture medium growth

[Ref.: #43840]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43840]

Culture medium growth

[Ref.: #43840]

Culture medium

Trypticase Soy Agar (TSA)

[Ref.: #43840]

Culture medium growth

[Ref.: #43840]

Culture medium

MacConkey agar

[Ref.: #43840]

Culture medium growth

		Temperature
[Ref.: #43840]	growth	18-37 °C
[Ref.: #43840]	optimum	25-37 °C
[Ref.: #67770]	growth	28 °C

[Ref.: #43840]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43840]			optimum	7
[Ref.: #43840]			growth	6-7.5

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43840]

Oxygen tolerance

facultative anaerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43840]		NaCl		growth	0-4	%(w/v)
[Ref.: #43840]		NaCl		optimum	0-2	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43840]	adipate ChEBI	-	assimilation
[Ref.: #43840]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #43840]	casein	-	hydrolysis
[Ref.: #43840]	chitin ChEBI	-	hydrolysis
[Ref.: #43840]	citrate ChEBI	-	assimilation
[Ref.: #43840]	D-glucose ChEBI	+	assimilation
[Ref.: #43840]	D-mannitol ChEBI	+	assimilation
[Ref.: #43840]	D-mannose ChEBI	+	assimilation
[Ref.: #43840]	decanoate ChEBI	-	assimilation
[Ref.: #43840]	dna ChEBI	+	hydrolysis
[Ref.: #43840]	esculin ChEBI	+	hydrolysis
[Ref.: #43840]	gelatin ChEBI	-	hydrolysis
[Ref.: #43840]	gluconate ChEBI	-	assimilation
[Ref.: #43840]	glucose ChEBI	+	builds acid from
[Ref.: #43840]	L-arabinose ChEBI	+	assimilation
[Ref.: #43840]	L-tyrosine ChEBI	-	hydrolysis
[Ref.: #43840]	malate ChEBI	-	assimilation
[Ref.: #43840]	maltose ChEBI	+	assimilation
[Ref.: #43840]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #43840]	nitrate ChEBI	-	reduction
[Ref.: #43840]	phenylacetate ChEBI	-	assimilation
[Ref.: #43840]	starch ChEBI	-	hydrolysis
[Ref.: #43840]	tween 20 ChEBI	+	hydrolysis
[Ref.: #43840]	tween 80 ChEBI	+	hydrolysis
[Ref.: #43840]	urea ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43840]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43840]	acid phosphatase	-	3.1.3.2
[Ref.: #43840]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43840]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43840]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43840]	alpha-galactosidase	+	3.2.1.22
[Ref.: #43840]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43840]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43840]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43840]	beta-galactosidase	+	3.2.1.23
[Ref.: #43840]	beta-glucosidase	+	3.2.1.21
[Ref.: #43840]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43840]	catalase	+	1.11.1.6
[Ref.: #43840]	cystine arylamidase	+	3.4.11.3
[Ref.: #43840]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43840]	esterase (C 4)	-
[Ref.: #43840]	esterase Lipase (C 8)	-
[Ref.: #43840]	leucine arylamidase	+	3.4.11.1
[Ref.: #43840]	lipase (C 14)	-
[Ref.: #43840]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43840]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #43840]	trypsin	+	3.4.21.4
[Ref.: #43840]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43840]

Fatty acid	Percentage
C15:0 anteiso	51.5
C16:0 iso	16.3
C16:0	6.6
C17:0 anteiso	6.6
C14:0 iso	5
C19:1ω9c / C19:1ω11c	2.5
C18:0	2.2
C15:0 iso	2
C12:0aldehyde	1.9
C20:1ω7c	1.6
C14:0	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43840]	Sample type/isolated from	Soil from the Guro-gu district
[Ref.: #43840]	Geographic location (country and/or sea, region)	Seoul
[Ref.: #43840]	Country	Republic of Korea
[Ref.: #43840]	Country ISO 3 Code	KOR
[Ref.: #43840]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Garden soil from the Guro-gu district of Seoul
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental

#Terrestrial

#Soil

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KM073966 (>99% sequence identity) for Brachybacterium from Microbeatlas

Aquatic Samples	136
Soil Samples	61
Animal Samples	1322
Plant Samples	64
Total Samples	1583

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43840]	16S rRNA gene sequence	KM073966		GenBank

[Ref.: #43840]

GC-content

69.5 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #43840]

Culture collection no.

KCTC 39563, CCTCC AB 2015116, JCM 31742

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Brachybacterium horti sp. nov., isolated from garden soil.	Singh H, Du J, Yang JE, Shik Yin C, Kook M, Yi TH	Int J Syst Evol Microbiol	10.1099/ijsem.0.000696	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43840

Hina Singh, Juan Du, Jung-Eun Yang, Chang Shik Yin, MooChang Kook, Tae-Hoo Yi: Brachybacterium horti sp. nov., isolated from garden soil. IJSEM 66: 189 - 195 2016 ( DOI 10.1099/ijsem.0.000696 , PubMed 26476895 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Brachybacterium horti in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF