Bartonella apis (NCIMB 14961, DSM 29779, PEB0122)

Name: Bartonella apis (NCIMB 14961, DSM 29779, PEB0122)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140945

Type strain

Strain Designation PEB0122

Culture col. no. NCIMB 14961 DSM 29779 PEB0122

NCBI tax ID(s) 1686310

Special Collections DSMZ Literature strains

Links

Bartonella apis PEB0122 is a mesophilic, Gram-negative, rod-shaped prokaryote that forms circular colonies and was isolated from The gut of a western honey bee, A. mellifera, from West Haven.

Gram-negative rod-shaped colony-forming mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 405015

LMG 33051,NCIMB 14961,DSM 29779

@ref 20215

Last LPSN update 2026-02-09 11:33:45

Domain Bacteria

Phylum Pseudomonadota

Class Alphaproteobacteria

Order Hyphomicrobiales

Family Bartonellaceae

Genus Bartonella

Species Bartonella apis

Full scientific name Bartonella apis Kešnerová et al. 2016

Synonyms (1)

"Ditibartonella apis"

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Confidence
43837	negative	1.2-1.8 µm	0.5 µm	rod-shaped
125438	negative				96.8

Colony morphology

@ref	Colony size	Colony color	Colony shape	Incubation period	Medium used
43837	0.2-0.4 mm	Opaque-white	circular	2-3 days	TSA supplemented with 5 % defibrinated sheep blood

Culture and growth conditions

Culture medium

@ref	Name	Medium link	Composition
43837	TSA supplemented with 5 % defibrinated sheep blood
66447	COLUMBIA BLOOD MEDIUM (DSMZ Medium 693)	Medium recipe at MediaDive	Name: COLUMBIA BLOOD MEDIUM (DSMZ Medium 693) Composition: Defibrinated sheep blood 50.0 g/l Columbia agar base
66447	TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545)	Medium recipe at MediaDive	Name: TRYPTONE SOYA BROTH (TSB) (DSMZ Medium 545) Composition: Casein peptone 17.0 g/l NaCl 5.0 g/l Soy peptone 3.0 g/l D(+)-Glucose 2.5 g/l K2HPO4 2.5 g/l Distilled water

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
66447	positive	growth	37	mesophilic

Physiology and metabolism

Spore formation

@ref	Spore formation	Confidence
125438		91.405

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
68369	17128 ChEBI	adipate	-	assimilation	from API 20NE
43837	22599 ChEBI	arabinose	+	fermentation
68369	29016 ChEBI	arginine	-	hydrolysis	from API 20NE
43837	16947 ChEBI	citrate	-	carbon source
68377	17634 ChEBI	D-glucose	+	builds acid from	from API NH
68369	17634 ChEBI	D-glucose	-	assimilation	from API 20NE
68369	17634 ChEBI	D-glucose	-	fermentation	from API 20NE
68369	16899 ChEBI	D-mannitol	-	assimilation	from API 20NE
68369	16024 ChEBI	D-mannose	-	assimilation	from API 20NE
68369	27689 ChEBI	decanoate	-	assimilation	from API 20NE
68369	4853 ChEBI	esculin	-	hydrolysis	from API 20NE
68369	5291 ChEBI	gelatin	-	hydrolysis	from API 20NE
68369	24265 ChEBI	gluconate	-	assimilation	from API 20NE
43837	17234 ChEBI	glucose	+	fermentation
68369	30849 ChEBI	L-arabinose	-	assimilation	from API 20NE
43837	17716 ChEBI	lactose	-	fermentation
68377	17306 ChEBI	maltose	-	builds acid from	from API NH
68369	17306 ChEBI	maltose	-	assimilation	from API 20NE
43837	29864 ChEBI	mannitol	-	fermentation
43837	17268 ChEBI	myo-inositol	-	fermentation
68369	59640 ChEBI	N-acetylglucosamine	-	assimilation	from API 20NE
68369	17632 ChEBI	nitrate	+	reduction	from API 20NE
43837	16634 ChEBI	raffinose	-	fermentation
43837	26546 ChEBI	rhamnose	-	fermentation
43837	15963 ChEBI	ribitol	-	fermentation
43837	17814 ChEBI	salicin	-	fermentation
43837	30911 ChEBI	sorbitol	-	fermentation
43837	17992 ChEBI	sucrose	-	fermentation
68377	17992 ChEBI	sucrose	-	builds acid from	from API NH
43837	27897 ChEBI	tryptophan	-	energy source
68377	27897 ChEBI	tryptophan	-	energy source	from API NH
68369	27897 ChEBI	tryptophan	-	energy source	from API 20NE
68369	16199 ChEBI	urea	+	hydrolysis	from API 20NE
43837	18222 ChEBI	xylose	+	fermentation

Antibiotic resistance

@ref	ChEBI	Metabolite	Sensitivity conc.
43837	28971	ampicillin	20 µg (disc)
43837	17698	chloramphenicol	20 µg (disc)
43837	48923	erythromycin	10 µg (disc)
43837	48923	erythromycin	20 µg (disc)
43837	17833	gentamicin	10 µg (disc)
43837	17833	gentamicin	20 µg (disc)
43837	6104	kanamycin	10 µg (disc)
43837	6104	kanamycin	20 µg (disc)
43837	6104	kanamycin	30 µg (disc)
43837	27701	oxytetracycline	20 µg (disc)
43837	28077	rifampicin	10 µg (disc)
43837	28077	rifampicin	20 µg (disc)
43837	28077	rifampicin	30 µg (disc)
43837	17076	streptomycin	10 µg (disc)
43837	17076	streptomycin	20 µg (disc)
43837	27902	tetracycline	20 µg (disc)

Metabolite production

@ref	Chebi-ID	Metabolite
43837	15688 ChEBI	acetoin
43837	16136 ChEBI	hydrogen sulfide
43837	35581 ChEBI	indole
68377	35581 ChEBI	indole	from API NH
68369	35581 ChEBI	indole	from API 20NE

Metabolite tests

@ref	Chebi-ID	Metabolite	Indole test	Voges-proskauer-test	Citrate test
43837	15688 ChEBI	acetoin		-
43837	16947 ChEBI	citrate			-
43837	35581 ChEBI	indole	-
68377	35581 ChEBI	indole	-			from API NH
68369	35581 ChEBI	indole	-			from API 20NE

Enzymes

@ref	Value	Activity	Ec
68377	alkaline phosphatase	-	3.1.3.1	from API NH
43837	arginine dihydrolase	-	3.5.3.6
68369	arginine dihydrolase	-	3.5.3.6	from API 20NE
43837	beta-galactosidase	-	3.2.1.23
68369	beta-glucosidase	-	3.2.1.21	from API 20NE
43837	catalase	+	1.11.1.6
43837	cytochrome oxidase	+	1.9.3.1
68369	gelatinase	-		from API 20NE
43837	lysine decarboxylase	-	4.1.1.18
43837	nitrate reductase	+	1.7.99.4
43837	ornithine decarboxylase	-	4.1.1.17
68377	proline-arylamidase	+	3.4.11.5	from API NH
43837	tryptophan deaminase	-	4.1.99.1
68377	tryptophan deaminase	-	4.1.99.1	from API NH
43837	urease	+	3.5.1.5
68369	urease	+	3.5.1.5	from API 20NE

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	coenzyme A metabolism	100	4 of 4
66794	cis-vaccenate biosynthesis	100	2 of 2
66794	adipate degradation	100	2 of 2
66794	ethanol fermentation	100	2 of 2
66794	palmitate biosynthesis	100	22 of 22
66794	C4 and CAM-carbon fixation	100	8 of 8
66794	gluconeogenesis	100	8 of 8
66794	formaldehyde oxidation	100	3 of 3
66794	acetate fermentation	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	acetoin degradation	100	3 of 3
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	folate polyglutamylation	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	methylglyoxal degradation	100	5 of 5
66794	valine metabolism	100	9 of 9
66794	ppGpp biosynthesis	100	4 of 4
66794	4-hydroxymandelate degradation	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	vitamin B12 metabolism	88.24	30 of 34
66794	propanol degradation	85.71	6 of 7
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	photosynthesis	85.71	12 of 14
66794	vitamin B1 metabolism	84.62	11 of 13
66794	Entner Doudoroff pathway	80	8 of 10
66794	threonine metabolism	80	8 of 10
66794	flavin biosynthesis	80	12 of 15
66794	peptidoglycan biosynthesis	80	12 of 15
66794	molybdenum cofactor biosynthesis	77.78	7 of 9
66794	lipid A biosynthesis	77.78	7 of 9
66794	phenylalanine metabolism	76.92	10 of 13
66794	butanoate fermentation	75	3 of 4
66794	lactate fermentation	75	3 of 4
66794	isoleucine metabolism	75	6 of 8
66794	CMP-KDO biosynthesis	75	3 of 4
66794	glutamate and glutamine metabolism	75	21 of 28
66794	vitamin B6 metabolism	72.73	8 of 11
66794	NAD metabolism	72.22	13 of 18
66794	citric acid cycle	71.43	10 of 14
66794	cardiolipin biosynthesis	71.43	5 of 7
66794	ubiquinone biosynthesis	71.43	5 of 7
66794	glycolysis	70.59	12 of 17
66794	propionate fermentation	70	7 of 10
66794	urea cycle	69.23	9 of 13
66794	leucine metabolism	69.23	9 of 13
66794	methionine metabolism	69.23	18 of 26
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	octane oxidation	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	d-mannose degradation	66.67	6 of 9
66794	serine metabolism	66.67	6 of 9
66794	selenocysteine biosynthesis	66.67	4 of 6
66794	allantoin degradation	66.67	6 of 9
66794	L-lactaldehyde degradation	66.67	2 of 3
66794	tetrahydrofolate metabolism	64.29	9 of 14
66794	heme metabolism	64.29	9 of 14
66794	oxidative phosphorylation	63.74	58 of 91
66794	proline metabolism	63.64	7 of 11
66794	pentose phosphate pathway	63.64	7 of 11
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	62.5	5 of 8
66794	ketogluconate metabolism	62.5	5 of 8
66794	pyrimidine metabolism	62.22	28 of 45
66794	alanine metabolism	62.07	18 of 29
66794	purine metabolism	61.7	58 of 94
66794	cysteine metabolism	61.11	11 of 18
66794	phenylacetate degradation (aerobic)	60	3 of 5
66794	histidine metabolism	58.62	17 of 29
66794	lipid metabolism	58.06	18 of 31
66794	glutathione metabolism	57.14	8 of 14
66794	non-pathway related	55.26	21 of 38
66794	isoprenoid biosynthesis	53.85	14 of 26
66794	aminopropanol phosphate biosynthesis	50	1 of 2
66794	kanosamine biosynthesis II	50	1 of 2
66794	dTDPLrhamnose biosynthesis	50	4 of 8
66794	tryptophan metabolism	50	19 of 38
66794	quinate degradation	50	1 of 2
66794	mannosylglycerate biosynthesis	50	1 of 2
66794	arginine metabolism	50	12 of 24
66794	sulfopterin metabolism	50	2 of 4
66794	lysine metabolism	50	21 of 42
66794	nitrate assimilation	44.44	4 of 9
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	methanofuran biosynthesis	40	2 of 5
66794	glycogen metabolism	40	2 of 5
66794	glycine metabolism	40	4 of 10
66794	lipoate biosynthesis	40	2 of 5
66794	arachidonate biosynthesis	40	2 of 5
66794	ethylmalonyl-CoA pathway	40	2 of 5
66794	polyamine pathway	39.13	9 of 23
66794	phosphatidylethanolamine bioynthesis	38.46	5 of 13
66794	tyrosine metabolism	35.71	5 of 14
66794	phenol degradation	35	7 of 20
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	cyanate degradation	33.33	1 of 3
66794	sphingosine metabolism	33.33	2 of 6
66794	pantothenate biosynthesis	33.33	2 of 6
66794	(5R)-carbapenem carboxylate biosynthesis	33.33	1 of 3
66794	ascorbate metabolism	31.82	7 of 22
66794	degradation of sugar alcohols	31.25	5 of 16
66794	phenylpropanoid biosynthesis	30.77	4 of 13
66794	arachidonic acid metabolism	27.78	5 of 18
66794	degradation of hexoses	27.78	5 of 18
66794	cholesterol biosynthesis	27.27	3 of 11
66794	d-xylose degradation	27.27	3 of 11
66794	3-phenylpropionate degradation	26.67	4 of 15
66794	cyclohexanol degradation	25	1 of 4
66794	toluene degradation	25	1 of 4
66794	degradation of pentoses	25	7 of 28
66794	biotin biosynthesis	25	1 of 4
66794	glycogen biosynthesis	25	1 of 4
66794	sulfate reduction	23.08	3 of 13

API 20NE

@ref	Reduction of nitratesNO3	TRP	GLU_ Ferm	ADH (Arg)	URE	ESC	GEL	PNPG	GLU_ Assim	ARA	MNE	MAN	NAG	MAL	GNT	CAP	ADI	MLT	CIT	PAC	OX
66447	+	-	-	-	+	-	-	-	-	-	-	-	-	-	-	-	-	+/-	-	-	not determinedn.d.

API NH

@ref	PEN	GLU	FRU	MAL	SAC	ODC	URE	LIP	PAL	beta GAL	ProA	GGT	IND
66447	-	+	+	-	-	-	+	-	-	-	+	+	-
66447	+	+	-	-	-	+	+/-	-	-	+	+	-	-
66447	-	+	+	-	-	-	+	+/-	-	-	+	+	-

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Host	#Arthropoda	#Insecta
#Host Body-Site	#Gastrointestinal tract	#Stomach

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent
43837	The gut of a western honey bee, A. mellifera, from West Haven	West Haven, Connecticut	USA	USA	North America
66447	gut of a Western honey bee Apis mellifera	Connecticut, West Haven (41° 15' 28.2'' N, 72° 59' 27.1'' W)	USA	USA	North America

Taxonmaps

69479

Global distribution of 16S sequence KP987884 (>99% sequence identity) for Bartonella apis subclade from Microbeatlas

Aquatic Samples	764
Soil Samples	1467
Animal Samples	3728
Plant Samples	388
Total Samples	6347

Interaction and safety

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	NCBI tax ID	Score
66792	ASM195207v1 assembly for Bartonella apis PEB0122	contig	GCA_001952075	1686310	67.49

16S sequences

@ref	Description	Accession	Database
43837	Bartonella apis strain PEB0122 16S ribosomal RNA gene, complete sequence	KP987884

GC content

@ref	GC-content (mol%)	Method
43837	45.5	genome sequence analysis
66447	45.5	sequence analysis

Genome-based predictions

Predictions

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Bartonella apis PEB0122
NCBI: GCA_001952075.1

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	no	96.80	yes
125438	anaerobic	anaerobicⓘ	no	91.87	no
125438	aerobic	aerobicⓘ	yes	70.04	no
125438	spore-forming	spore-formingⓘ	no	91.41	no
125438	thermophilic	thermophileⓘ	no	95.68	no
125438	flagellated	motile2+ⓘ	no	53.77	no

Literature

Topic	Title	Authors	Journal	DOI	Year
Enzymology	Cloning, Expression and Characterization of a Highly Active Alcohol Dehydrogenase for Production of Ethyl (S)-4-Chloro-3-Hydroxybutyrate.	Zhu YH, Liu CY, Cai S, Guo LB, Kim IW, Kalia VC, Lee JK, Zhang YW.	Indian J Microbiol	10.1007/s12088-019-00795-0	2019
	The Gut Microbiota Can Provide Viral Tolerance in the Honey Bee.	Dosch C, Manigk A, Streicher T, Tehel A, Paxton RJ, Tragust S.	Microorganisms	10.3390/microorganisms9040871	2021
Pathogenicity	Characterization of Biofilm Producer Nanobacteria Isolated from Kidney Stones of Some Egyptian Patients.	Fayez Hassan N, Khaled Ibrahim M, Yousef El Tablawy S, Abd Allah Farrag H	Pak J Biol Sci	10.3923/pjbs.2021.953.970	2021
Phylogeny	Bartonella apis sp. nov., a honey bee gut symbiont of the class Alphaproteobacteria.	Kesnerova L, Moritz R, Engel P	Int J Syst Evol Microbiol	10.1099/ijsem.0.000736	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43837	Lucie Kesnerová Roxane Moritz, Philipp Engel: Bartonella apis sp. nov., a honey bee gut symbiont of the class Alphaproteobacteria. IJSEM 66: 414 - 421 2016 ( DOI 10.1099/ijsem.0.000736 , PubMed 26537852 )
#66447	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 29779
#66792	Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#68369	Automatically annotated from API 20NE .
#68377	Automatically annotated from API NH .
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

Rate our new design

Content

About us

Bartonella apis (NCIMB 14961, DSM 29779, PEB0122)

Name and taxonomic classification

Morphology

Cell morphology

Colony morphology

Culture and growth conditions

Culture medium

Culture temp

Physiology and metabolism

Spore formation

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

Pathway annotation

API 20NE

API NH

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

Genome sequences

Genome browser: GCA_001952075

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

BacDive

Help

Social Media