Name: Paenibacillus radicis 694
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140933

Type strain:

Species: Paenibacillus radicis

Strain Designation: 694

Culture col. no.: CGMCC 1.15286, DSM 100762

Strain history: <- J. Gao, Beijing Agro-Biotechnology Research Center; 694

NCBI tax ID(s): 1737354 (species)

SI-ID 400113

DSM 100762

Section

Paenibacillus radicis 694 is an aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from Surface-sterilized root tissue of maize planted in Fangshan District of Beijing.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Paenibacillaceae*
	Genus	*Paenibacillus*
	Species	**Paenibacillus radicis**
	Full Scientific Name (LPSN)	Paenibacillus radicis Gao et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43813]	Gram stain	positive
[Ref.: #69480]	Gram stain	positive Confidence in %100

[Ref.: #43813]	Cell length	3.8-4.4 µm

[Ref.: #43813]	Cell width	0.8-1.3 µm

[Ref.: #43813]	Cell shape	rod-shaped

[Ref.: #43813]	Motility	yes
[Ref.: #69480]	Motility	yes Confidence in %95.639

[Ref.: #43813]	Flagellum arrangement	peritrichous

[Ref.: #43813]	Colony size	0.5-1 mm
[Ref.: #43813]	Colony color	Milky, glossy
[Ref.: #43813]	Colony shape	circular
[Ref.: #43813]	Incubation period	3 days
[Ref.: #43813]	Cultivation medium used	R2A agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43813]

Culture medium

Reasoner's 2A agar (R2A)

[Ref.: #43813]

Culture medium growth

[Ref.: #67652]

Culture medium

R2A MEDIUM (DSMZ Medium 830)

[Ref.: #67652]

Culture medium growth

[Ref.: #67652]

Culture medium link

Medium recipe at MediaDive

[Ref.: #67652]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #43813]	growth	10-45 °C
[Ref.: #43813]	optimum	30 °C
[Ref.: #67652]	growth	30 °C

[Ref.: #43813]	Temperature range	mesophilic
[Ref.: #67652]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43813]			optimum	7
[Ref.: #43813]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43813]

Oxygen tolerance

aerobe

[Ref.: #43813]	Spore description	Ellipsoidal in a subterminal position
[Ref.: #43813]	Type of spore	endospore
[Ref.: #43813]	Ability of spore formation	yes
[Ref.: #69481]	Ability of spore formation	yes Confidence in %100
[Ref.: #69480]	Ability of spore formation	yes Confidence in %100

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43813]		NaCl		growth	0-3	%(w/v)
[Ref.: #43813]		NaCl		optimum	1	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43813]	1 % sodium lactate	+	growth
[Ref.: #43813]	acetate ChEBI	-	growth
[Ref.: #43813]	acetoacetate ChEBI	-	growth
[Ref.: #43813]	adipate ChEBI	-	assimilation
[Ref.: #43813]	alpha-D-glucose ChEBI	-	growth
[Ref.: #43813]	alpha-lactose ChEBI	+	growth
[Ref.: #43813]	amygdalin ChEBI	+	builds acid from
[Ref.: #43813]	arbutin ChEBI	+	builds acid from
[Ref.: #43813]	casein	-	hydrolysis
[Ref.: #43813]	cellobiose ChEBI	+	builds acid from
[Ref.: #43813]	cellobiose ChEBI	+	growth
[Ref.: #43813]	D-fructose ChEBI	-	growth
[Ref.: #43813]	D-fructose ChEBI	+	builds acid from
[Ref.: #43813]	D-fructose 6-phosphate ChEBI	+	growth
[Ref.: #43813]	D-galactose ChEBI	+	builds acid from
[Ref.: #43813]	D-galactose ChEBI	+	growth
[Ref.: #43813]	D-glucose ChEBI	+	builds acid from
[Ref.: #43813]	D-glucose 6-phosphate ChEBI	+	growth
[Ref.: #43813]	D-lyxose ChEBI	+	builds acid from
[Ref.: #43813]	D-mannitol ChEBI	+	assimilation
[Ref.: #43813]	D-mannitol ChEBI	+	builds acid from
[Ref.: #43813]	D-mannitol ChEBI	+	growth
[Ref.: #43813]	D-mannose ChEBI	+	assimilation
[Ref.: #43813]	D-mannose ChEBI	+	builds acid from
[Ref.: #43813]	D-mannose ChEBI	+	growth
[Ref.: #43813]	D-xylose ChEBI	+	builds acid from
[Ref.: #43813]	decanoate ChEBI	-	assimilation
[Ref.: #43813]	esculin ChEBI	+	hydrolysis
[Ref.: #43813]	esculin ferric citrate	+	builds acid from
[Ref.: #43813]	gelatin ChEBI	-	hydrolysis
[Ref.: #43813]	gentiobiose ChEBI	+	builds acid from
[Ref.: #43813]	gentiobiose ChEBI	+	growth
[Ref.: #43813]	glucose ChEBI	-	assimilation
[Ref.: #43813]	glucose ChEBI	-	fermentation
[Ref.: #43813]	glycogen ChEBI	-	builds acid from
[Ref.: #43813]	L-arabinose ChEBI	+	assimilation
[Ref.: #43813]	L-aspartate ChEBI	-	growth
[Ref.: #43813]	L-fucose ChEBI	+	growth
[Ref.: #43813]	lactose ChEBI	+	builds acid from
[Ref.: #43813]	malate ChEBI	-	assimilation
[Ref.: #43813]	malate ChEBI	+	growth
[Ref.: #43813]	maltose ChEBI	+	assimilation
[Ref.: #43813]	maltose ChEBI	+	builds acid from
[Ref.: #43813]	melezitose ChEBI	+/-	builds acid from
[Ref.: #43813]	melibiose ChEBI	+	builds acid from
[Ref.: #43813]	melibiose ChEBI	+	growth
[Ref.: #43813]	methyl alpha-D-glucopyranoside ChEBI	+	builds acid from
[Ref.: #43813]	methyl alpha-D-mannoside ChEBI	+	builds acid from
[Ref.: #43813]	methyl beta-D-xylopyranoside ChEBI	+	builds acid from
[Ref.: #43813]	methyl D-glucoside ChEBI	+	growth
[Ref.: #43813]	N-acetylglucosamine ChEBI	+	assimilation
[Ref.: #43813]	N-acetylglucosamine ChEBI	+	builds acid from
[Ref.: #43813]	N-acetylglucosamine ChEBI	+	growth
[Ref.: #43813]	nalidixic acid ChEBI	-	growth
[Ref.: #43813]	nitrate ChEBI	-	respiration
[Ref.: #43813]	pectin ChEBI	-	growth
[Ref.: #43813]	phenylacetate ChEBI	-	assimilation
[Ref.: #43813]	potassium gluconate ChEBI	-	assimilation
[Ref.: #43813]	potassium tellurite ChEBI	+	growth
[Ref.: #43813]	raffinose ChEBI	+	builds acid from
[Ref.: #43813]	raffinose ChEBI	+	growth
[Ref.: #43813]	salicin ChEBI	+	builds acid from
[Ref.: #43813]	salicin ChEBI	+	growth
[Ref.: #43813]	sodium butyrate ChEBI	-	growth
[Ref.: #43813]	sodium citrate ChEBI	-	assimilation
[Ref.: #43813]	stachyose ChEBI	+	growth
[Ref.: #43813]	starch ChEBI	-	builds acid from
[Ref.: #43813]	starch ChEBI	-	hydrolysis
[Ref.: #43813]	sucrose ChEBI	+	builds acid from
[Ref.: #43813]	sucrose ChEBI	+	growth
[Ref.: #43813]	tetrazolium violet ChEBI	+	growth
[Ref.: #43813]	trehalose ChEBI	+	builds acid from
[Ref.: #43813]	trehalose ChEBI	+	growth
[Ref.: #43813]	turanose ChEBI	+	builds acid from
[Ref.: #43813]	turanose ChEBI	+	growth
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43813]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43813]	acid phosphatase	+	3.1.3.2
[Ref.: #43813]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43813]	alpha-chymotrypsin	-	3.4.21.1
[Ref.: #43813]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43813]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43813]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43813]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43813]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43813]	beta-galactosidase	+	3.2.1.23
[Ref.: #43813]	beta-glucosidase	-	3.2.1.21
[Ref.: #43813]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43813]	catalase	+	1.11.1.6
[Ref.: #43813]	cystine arylamidase	-	3.4.11.3
[Ref.: #43813]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43813]	esterase (C 4)	-
[Ref.: #43813]	esterase Lipase (C 8)	+
[Ref.: #43813]	leucine arylamidase	+	3.4.11.1
[Ref.: #43813]	lipase (C 14)	+
[Ref.: #43813]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43813]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43813]	trypsin	+	3.4.21.4
[Ref.: #43813]	urease	-	3.5.1.5
[Ref.: #43813]	valine arylamidase	-
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43813]	Sample type/isolated from	Surface-sterilized root tissue of maize planted in Fangshan District of Beijing
[Ref.: #43813]	Geographic location (country and/or sea, region)	Beijing
[Ref.: #43813]	Country	China
[Ref.: #43813]	Country ISO 3 Code	CHN
[Ref.: #43813]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root (Rhizome)
#Host Body-Site	#Plant	#Sterilized plant part

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence KR610523 (>99% sequence identity) for Paenibacillus from Microbeatlas

Aquatic Samples	18
Soil Samples	276
Animal Samples	68
Plant Samples	93
Total Samples	455

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #67652]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43813]	16S rRNA gene sequence	KR610523		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Paenibacillus radicis CGMCC 1.15286	GCA_014641555	scaffold	GenBank	1737354 tax ID	*
[Ref.: #66792]	Paenibacillus radicis strain CGMCC 1.15286	1737354.3	wgs	PATRIC	1737354 tax ID	*

[Ref.: #67652]

GC-content

46.9 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Paenibacillus radicis CGMCC 1.15286 NCBI: GCA_014641555.1
[Ref.: #69481]	spore-forming	yes	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Paenibacillus radicis CGMCC 1.15286 NCBI: GCA_014641555.1
[Ref.: #69480]	flagellated	yes	88.583	yes
[Ref.: #69480]	gram-positive	yes	89.381	no
[Ref.: #69480]	anaerobic	no	99.206	yes
[Ref.: #69480]	aerobic	yes	88.227	yes
[Ref.: #69480]	halophile	no	94.854	no
[Ref.: #69480]	spore-forming	yes	94.892	no
[Ref.: #69480]	thermophile	no	99.901	no
[Ref.: #69480]	glucose-util	yes	82.427	yes
[Ref.: #69480]	motile	yes	89.919	yes
[Ref.: #69480]	glucose-ferment	no	87.746	no

Availability in culture collections External links

[Ref.: #67652]

Culture collection no.

CGMCC 1.15286, DSM 100762

[Ref.: #96816]

SI-ID 400113

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Paenibacillus radicis sp. nov., an endophytic bacterium isolated from maize root.	Gao JL, Yuan M, Wang XM, Qiu TL, Lv FY, Yang MM, Sun JG	Int J Syst Evol Microbiol	10.1099/ijsem.0.000795	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43813

Jun-Lian Gao, Mei Yuan, Xu-Ming Wang, Tian-Lei Qiu, Fan-Yang Lv, Ming-Ming Yang, Jian-Guang Sun: Paenibacillus radicis sp. nov., an endophytic bacterium isolated from maize root. IJSEM 66: 807 - 811 2016 ( DOI 10.1099/ijsem.0.000795 , PubMed 26612690 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67652

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 100762

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

#96816

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID400113.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Paenibacillus radicis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF

Paenibacillus radicis CGMCC 1.15286

Paenibacillus radicis CGMCC 1.15286

Change proposal

Change proposal