Flexilinea flocculi (JCM 30897, CGMCC 1.5202, TC 1)

Name: Flexilinea flocculi (JCM 30897, CGMCC 1.5202, TC 1)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 140919

Type strain

Culture col. no. JCM 30897 CGMCC 1.5202 TC 1

NCBI tax ID(s) 1678840

Special Collections JCM Literature strains

History Y. Sekiguchi; Biomed. Res. Inst., AIST, Japan; TC1.

Links

Flexilinea flocculi JCM 30897 is an obligate anaerobe, chemoorganotroph, Gram-negative bacterium that was isolated from A methanogenic granular sludge in a full-scale mesophilic UASB reactor treating high-strength starch-based organic wastewater.

Gram-negative filament-shaped obligate anaerobe chemoorganotroph 16S sequence Bacteria

Name and taxonomic classification

SI-ID 393453

JCM 30897

@ref 20215

Last LPSN update 2026-02-09 11:33:45

Domain Bacteria

Phylum Chloroflexota

Class Anaerolineae

Order [Anaerolineae, not assigned to order]

Family [Anaerolineae, not assigned to family]

Genus Flexilinea

Species Flexilinea flocculi

Full scientific name Flexilinea flocculi Sun et al. 2016

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility
43788	negative	100 µm	0.3-0.4 µm	filament-shaped

Culture and growth conditions

Culture temp

@ref	Growth	Type	Temperature (°C)
43788	positive	growth	25-43
43788	positive	optimum	37
67770	positive	growth	37

Culture pH

@ref	Ability	Type	PH	PH range
43788	positive	optimum	7
43788	positive	growth	6-8.5	alkaliphile

Physiology and metabolism

Oxygen tolerance

43788

Oxygen toleranceobligate anaerobe

Nutrition type

43788

Typechemoorganotroph

Halophily

@ref	Salt	Growth	Tested relation	Concentration
43788	NaCl	positive	optimum	0 g/L
43788	NaCl	positive	growth	0-10 g/L

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
43788	30089 ChEBI	acetate	-	assimilation
43788	22599 ChEBI	arabinose	+	growth
43788	16150 ChEBI	benzoate	-	assimilation
43788	17750 ChEBI	betaine	-	assimilation
43788	17750 ChEBI	betaine	-	growth
43788	17968 ChEBI	butyrate	-	assimilation
43788		casamino acids	-	assimilation
43788	35899 ChEBI	crotonate	-	assimilation
43788	18276 ChEBI	dihydrogen	-	assimilation
43788	33403 ChEBI	elemental sulfur	-	electron acceptor
43788	16236 ChEBI	ethanol	-	assimilation
43788	29034 ChEBI	ferric iron	-	electron acceptor
43788	28757 ChEBI	fructose	+	growth
43788	29806 ChEBI	fumarate	-	assimilation
43788	29806 ChEBI	fumarate	-	electron acceptor
43788	28260 ChEBI	galactose	-	growth
43788	17234 ChEBI	glucose	+	growth
43788	15978 ChEBI	glycerol 3-phosphate	-	assimilation
43788	24996 ChEBI	lactate	-	assimilation
43788	25115 ChEBI	malate	-	assimilation
43788	37684 ChEBI	mannose	-	growth
43788	17790 ChEBI	methanol	-	assimilation
43788	17632 ChEBI	nitrate	-	electron acceptor
43788	17309 ChEBI	pectin	-	growth
43788		peptone	-	assimilation
43788	46793 ChEBI	polyethylene glycol	-	assimilation
43788	28831 ChEBI	propanol	-	assimilation
43788	17272 ChEBI	propionate	-	assimilation
43788	15361 ChEBI	pyruvate	+	growth
43788	16634 ChEBI	raffinose	-	assimilation
43788	33942 ChEBI	ribose	+	growth
43788	28017 ChEBI	starch	+	growth
43788	30031 ChEBI	succinate	-	assimilation
43788	17992 ChEBI	sucrose	-	assimilation
43788	16189 ChEBI	sulfate	-	electron acceptor
43788	17359 ChEBI	sulfite	-	electron acceptor
43788	16094 ChEBI	thiosulfate	-	electron acceptor
43788		tryptone	-	growth
43788	37166 ChEBI	xylan	-	assimilation
43788	18222 ChEBI	xylose	+	growth
43788		yeast extract	+	required for growth

Pathway annotation

Computationally determined by

@ref	pathway	enzyme coverage	annotated reactions
66794	gluconeogenesis	100	8 of 8
66794	vitamin E metabolism	100	4 of 4
66794	kanosamine biosynthesis II	100	2 of 2
66794	metabolism of amino sugars and derivatives	100	5 of 5
66794	acetoin degradation	100	3 of 3
66794	coenzyme A metabolism	100	4 of 4
66794	CDP-diacylglycerol biosynthesis	100	2 of 2
66794	UDP-GlcNAc biosynthesis	100	3 of 3
66794	folate polyglutamylation	100	1 of 1
66794	suberin monomers biosynthesis	100	2 of 2
66794	ceramide biosynthesis	100	1 of 1
66794	anapleurotic synthesis of oxalacetate	100	1 of 1
66794	L-lactaldehyde degradation	100	3 of 3
66794	serine metabolism	88.89	8 of 9
66794	chorismate metabolism	88.89	8 of 9
66794	valine metabolism	88.89	8 of 9
66794	C4 and CAM-carbon fixation	87.5	7 of 8
66794	reductive acetyl coenzyme A pathway	85.71	6 of 7
66794	hydrogen production	80	4 of 5
66794	starch degradation	80	8 of 10
66794	methylglyoxal degradation	80	4 of 5
66794	threonine metabolism	80	8 of 10
66794	glycine betaine biosynthesis	80	4 of 5
66794	myo-inositol biosynthesis	80	8 of 10
66794	peptidoglycan biosynthesis	80	12 of 15
66794	glycogen metabolism	80	4 of 5
66794	cellulose degradation	80	4 of 5
66794	pyrimidine metabolism	77.78	35 of 45
66794	d-mannose degradation	77.78	7 of 9
66794	palmitate biosynthesis	77.27	17 of 22
66794	phenylalanine metabolism	76.92	10 of 13
66794	glycolysis	76.47	13 of 17
66794	isoleucine metabolism	75	6 of 8
66794	ketogluconate metabolism	75	6 of 8
66794	6-hydroxymethyl-dihydropterin diphosphate biosynthesis	75	6 of 8
66794	dTDPLrhamnose biosynthesis	75	6 of 8
66794	acetate fermentation	75	3 of 4
66794	ppGpp biosynthesis	75	3 of 4
66794	glycogen biosynthesis	75	3 of 4
66794	pentose phosphate pathway	72.73	8 of 11
66794	photosynthesis	71.43	10 of 14
66794	degradation of pentoses	71.43	20 of 28
66794	Entner Doudoroff pathway	70	7 of 10
66794	degradation of sugar alcohols	68.75	11 of 16
66794	purine metabolism	68.09	64 of 94
66794	aspartate and asparagine metabolism	66.67	6 of 9
66794	CO2 fixation in Crenarchaeota	66.67	6 of 9
66794	formaldehyde oxidation	66.67	2 of 3
66794	octane oxidation	66.67	2 of 3
66794	glycolate and glyoxylate degradation	66.67	4 of 6
66794	NAD metabolism	66.67	12 of 18
66794	oxidative phosphorylation	65.93	60 of 91
66794	non-pathway related	65.79	25 of 38
66794	glutamate and glutamine metabolism	64.29	18 of 28
66794	d-xylose degradation	63.64	7 of 11
66794	histidine metabolism	62.07	18 of 29
66794	methionine metabolism	57.69	15 of 26
66794	tyrosine metabolism	57.14	8 of 14
66794	tetrahydrofolate metabolism	57.14	8 of 14
66794	ubiquinone biosynthesis	57.14	4 of 7
66794	propanol degradation	57.14	4 of 7
66794	cardiolipin biosynthesis	57.14	4 of 7
66794	degradation of hexoses	55.56	10 of 18
66794	alanine metabolism	55.17	16 of 29
66794	sulfopterin metabolism	50	2 of 4
66794	CMP-KDO biosynthesis	50	2 of 4
66794	ethanol fermentation	50	1 of 2
66794	lactate fermentation	50	2 of 4
66794	cis-vaccenate biosynthesis	50	1 of 2
66794	butanoate fermentation	50	2 of 4
66794	selenocysteine biosynthesis	50	3 of 6
66794	cysteine metabolism	50	9 of 18
66794	adipate degradation	50	1 of 2
66794	glycine metabolism	50	5 of 10
66794	leucine metabolism	46.15	6 of 13
66794	urea cycle	46.15	6 of 13
66794	sulfate reduction	46.15	6 of 13
66794	metabolism of disaccharids	45.45	5 of 11
66794	molybdenum cofactor biosynthesis	44.44	4 of 9
66794	lipid A biosynthesis	44.44	4 of 9
66794	degradation of sugar acids	44	11 of 25
66794	citric acid cycle	42.86	6 of 14
66794	lipid metabolism	41.94	13 of 31
66794	degradation of aromatic, nitrogen containing compounds	41.67	5 of 12
66794	arginine metabolism	41.67	10 of 24
66794	lipoate biosynthesis	40	2 of 5
66794	coenzyme M biosynthesis	40	4 of 10
66794	propionate fermentation	40	4 of 10
66794	creatinine degradation	40	2 of 5
66794	tryptophan metabolism	39.47	15 of 38
66794	vitamin B1 metabolism	38.46	5 of 13
66794	proline metabolism	36.36	4 of 11
66794	ascorbate metabolism	36.36	8 of 22
66794	arachidonic acid metabolism	33.33	6 of 18
66794	sphingosine metabolism	33.33	2 of 6
66794	acetyl CoA biosynthesis	33.33	1 of 3
66794	IAA biosynthesis	33.33	1 of 3
66794	nitrate assimilation	33.33	3 of 9
66794	enterobactin biosynthesis	33.33	1 of 3
66794	flavin biosynthesis	33.33	5 of 15
66794	carotenoid biosynthesis	31.82	7 of 22
66794	androgen and estrogen metabolism	31.25	5 of 16
66794	lysine metabolism	30.95	13 of 42
66794	isoprenoid biosynthesis	30.77	8 of 26
66794	mevalonate metabolism	28.57	2 of 7
66794	glutathione metabolism	28.57	4 of 14
66794	dolichyl-diphosphooligosaccharide biosynthesis	27.27	3 of 11
66794	carnitine metabolism	25	2 of 8
66794	toluene degradation	25	1 of 4
66794	biotin biosynthesis	25	1 of 4
66794	methanogenesis from CO2	25	3 of 12
66794	alginate biosynthesis	25	1 of 4
66794	cyclohexanol degradation	25	1 of 4
66794	phosphatidylethanolamine bioynthesis	23.08	3 of 13
66794	phenylpropanoid biosynthesis	23.08	3 of 13
66794	heme metabolism	21.43	3 of 14

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Condition	#Anoxic (anaerobic)	-
#Engineered	#Biodegradation	#Anaerobic digestor
#Engineered	#Bioreactor	-
#Engineered	#Waste	#Wastewater

Isolation

@ref	Sample type
43788	A methanogenic granular sludge in a full-scale mesophilic UASB reactor treating high-strength starch-based organic wastewater
67770	Methanogenic granular sludge in a full-scale USAB reactor treating high-strength starch-based organic wastewater

Taxonmaps

69479

Global distribution of 16S sequence LC049585 (>99% sequence identity) for Flexilinea flocculi subclade from Microbeatlas

Aquatic Samples	464
Soil Samples	43
Animal Samples	150
Plant Samples	47
Total Samples	704

Interaction and safety

Sequence information

16S sequences

@ref	Description	Accession	Database
43788	Flexilinea flocculi gene for 16S ribosomal RNA, partial sequence	LC049585

GC content

@ref	GC-content (mol%)	Method
67770	42.1	high performance liquid chromatography (HPLC)

Genome-based predictions

Literature

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Isolation and characterization of Flexilinea flocculi gen. nov., sp. nov., a filamentous, anaerobic bacterium belonging to the class Anaerolineae in the phylum Chloroflexi.	Sun L, Toyonaga M, Ohashi A, Matsuura N, Tourlousse DM, Meng XY, Tamaki H, Hanada S, Cruz R, Yamaguchi T, Sekiguchi Y	Int J Syst Evol Microbiol	10.1099/ijsem.0.000822	2015

References

#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#43788	Liwei Sun, Mayu Toyonaga, Akiko Ohashi, Norihisa Matsuura, Dieter M. Tourlousse, Xian-Ying Meng, Hideyuki Tamaki, Satoshi Hanada, Rodrigo Cruz, Takashi Yamaguchi, Yuji Sekiguchi: Isolation and characterization of Flexilinea flocculi gen. nov., sp. nov., a filamentous, anaerobic bacterium belonging to the class Anaerolineae in the phylum Chloroflexi. IJSEM 66: 988 - 996 2016 ( DOI 10.1099/ijsem.0.000822 , PubMed 26637817 )
#66794	Antje Chang, Lisa Jeske, Sandra Ulbrich, Julia Hofmann, Julia Koblitz, Ida Schomburg, Meina Neumann-Schaal, Dieter Jahn, Dietmar Schomburg: BRENDA, the ELIXIR core data resource in 2021: new developments and updates. Nucleic Acids Res. 49: D498 - D508 2020 ( DOI 10.1093/nar/gkaa1025 , PubMed 33211880 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Flexilinea flocculi (JCM 30897, CGMCC 1.5202, TC 1)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Nutrition type

Halophily

Metabolite utilization

Pathway annotation

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Sequence information

16S sequences

GC content

Genome-based predictions

Literature

Literature

References

BacDive

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