Name: Rhizobium puerariae PC004
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140905

Type strain:

Species: Rhizobium puerariae

Strain Designation: PC004

Culture col. no.: BCC 73740, NBRC 110722

NCBI tax ID(s): 1585791 (species)

Section

Rhizobium puerariae PC004 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from Root nodules of the Thai medicinal plant Pueraria candollei var. candollei grown at the Faculty of Pharmacy Mahidol University.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Hyphomicrobiales*
	Family	*Rhizobiaceae*
	Genus	*Rhizobium*
	Species	**Rhizobium puerariae**
	Full Scientific Name (LPSN)	Rhizobium puerariae Boonsnongcheep et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43749]	Gram stain	negative

[Ref.: #43749]	Cell length	1.7-2.4 µm

[Ref.: #43749]	Cell width	1-1.2 µm

[Ref.: #43749]	Cell shape	rod-shaped

[Ref.: #43749]	Motility	yes

[Ref.: #43749]	Colony size	0.5-1 mm
[Ref.: #43749]	Colony color	White
[Ref.: #43749]	Colony shape	circular
[Ref.: #43749]	Incubation period	3 days
[Ref.: #43749]	Cultivation medium used	YMA

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Trypticase Soy Agar (TSA)

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Culture medium growth

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Culture medium

YMA

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #43749]			growth	14-38 °C

	pH	Kind of pH		pH
[Ref.: #43749]			growth	6-12

Information on physiology and metabolism Physiology and metabolism

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Oxygen tolerance

aerobe

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Ability of spore formation

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43749]		NaCl		growth	0-2	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43749]	2-dehydro-D-gluconate ChEBI	-	carbon source
[Ref.: #43749]	5-dehydro-D-gluconate ChEBI	-	carbon source
[Ref.: #43749]	amygdalin ChEBI	-	carbon source
[Ref.: #43749]	arbutin ChEBI	-	carbon source
[Ref.: #43749]	cellobiose ChEBI	-	assimilation
[Ref.: #43749]	cellobiose ChEBI	-	carbon source
[Ref.: #43749]	D-arabinose ChEBI	+	carbon source
[Ref.: #43749]	D-arabitol ChEBI	+	carbon source
[Ref.: #43749]	D-fructose ChEBI	+	carbon source
[Ref.: #43749]	D-fucose ChEBI	+	carbon source
[Ref.: #43749]	D-galactose ChEBI	+	carbon source
[Ref.: #43749]	D-glucose ChEBI	+	carbon source
[Ref.: #43749]	D-lyxose ChEBI	+	carbon source
[Ref.: #43749]	D-mannitol ChEBI	+	carbon source
[Ref.: #43749]	D-mannose ChEBI	+	carbon source
[Ref.: #43749]	D-ribose ChEBI	+	carbon source
[Ref.: #43749]	D-sorbitol ChEBI	-	carbon source
[Ref.: #43749]	D-tagatose ChEBI	-	assimilation
[Ref.: #43749]	D-tagatose ChEBI	-	carbon source
[Ref.: #43749]	D-xylose ChEBI	+	carbon source
[Ref.: #43749]	erythritol ChEBI	-	carbon source
[Ref.: #43749]	esculin ChEBI	+	hydrolysis
[Ref.: #43749]	esculin ferric citrate	+	carbon source
[Ref.: #43749]	galactitol ChEBI	-	carbon source
[Ref.: #43749]	gelatin ChEBI	-	hydrolysis
[Ref.: #43749]	gentiobiose ChEBI	-	carbon source
[Ref.: #43749]	gluconate ChEBI	-	carbon source
[Ref.: #43749]	glycerol ChEBI	+	assimilation
[Ref.: #43749]	glycerol 3-phosphate ChEBI	+	carbon source
[Ref.: #43749]	glycogen ChEBI	-	carbon source
[Ref.: #43749]	inulin ChEBI	-	carbon source
[Ref.: #43749]	L-arabinose ChEBI	+	carbon source
[Ref.: #43749]	L-arabitol ChEBI	-	carbon source
[Ref.: #43749]	L-fucose ChEBI	+	carbon source
[Ref.: #43749]	L-rhamnose ChEBI	+	carbon source
[Ref.: #43749]	L-sorbose ChEBI	-	carbon source
[Ref.: #43749]	L-xylose ChEBI	-	assimilation
[Ref.: #43749]	L-xylose ChEBI	-	carbon source
[Ref.: #43749]	lactose ChEBI	-	carbon source
[Ref.: #43749]	malate ChEBI	+	assimilation
[Ref.: #43749]	malate ChEBI	+	carbon source
[Ref.: #43749]	maltose ChEBI	-	carbon source
[Ref.: #43749]	melezitose ChEBI	-	carbon source
[Ref.: #43749]	melibiose ChEBI	-	assimilation
[Ref.: #43749]	melibiose ChEBI	-	carbon source
[Ref.: #43749]	methyl alpha-D-glucopyranoside ChEBI	-	carbon source
[Ref.: #43749]	methyl alpha-D-mannoside ChEBI	-	carbon source
[Ref.: #43749]	methyl beta-D-xylopyranoside ChEBI	+	carbon source
[Ref.: #43749]	myo-inositol ChEBI	-	carbon source
[Ref.: #43749]	N-acetylglucosamine ChEBI	-	carbon source
[Ref.: #43749]	nitrate ChEBI	+	reduction
[Ref.: #43749]	raffinose ChEBI	-	carbon source
[Ref.: #43749]	ribitol ChEBI	-	assimilation
[Ref.: #43749]	ribitol ChEBI	-	carbon source
[Ref.: #43749]	salicin ChEBI	-	carbon source
[Ref.: #43749]	starch ChEBI	-	carbon source
[Ref.: #43749]	sucrose ChEBI	-	carbon source
[Ref.: #43749]	trehalose ChEBI	-	carbon source
[Ref.: #43749]	turanose ChEBI	-	assimilation
[Ref.: #43749]	turanose ChEBI	-	carbon source
[Ref.: #43749]	xylitol ChEBI	-	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic resistance	Concentration
[Ref.: #43749]	ampicillin ChEBI	yes	5	µg/mL
[Ref.: #43749]	chloramphenicol ChEBI	yes	50	µg/mL
[Ref.: #43749]	erythromycin ChEBI	yes	100	µg/mL
[Ref.: #43749]	kanamycin ChEBI	yes	50	µg/mL
[Ref.: #43749]	streptomycin ChEBI	yes	50	µg/mL
[Ref.: #43749]	streptomycin ChEBI	yes	100	µg/mL

	Metabolite production	Metabolite	Production
[Ref.: #43749]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43749]	arginine dihydrolase	-	3.5.3.6
[Ref.: #43749]	beta-galactosidase	+	3.2.1.23
[Ref.: #43749]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43749]	urease	+	3.5.1.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43749]	Sample type/isolated from	Root nodules of the Thai medicinal plant Pueraria candollei var. candollei grown at the Faculty of Pharmacy Mahidol University
[Ref.: #43749]	Geographic location (country and/or sea, region)	Bangkok
[Ref.: #43749]	Country	Thailand
[Ref.: #43749]	Country ISO 3 Code	THA
[Ref.: #43749]	Continent	Asia
[Ref.: #43749]	Isolation date	2010-12
* marker position based on {}

Isolation sources categories

#Host	#Plants	#Herbaceous plants (Grass,Crops)
#Host Body-Site	#Plant	#Root nodule

What are isolation sources categories?

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Global distribution of 16S sequence LC014930 (>99% sequence identity) for Rhizobium puerariae subclade from Microbeatlas

Aquatic Samples	7
Soil Samples	2
Animal Samples	15
Plant Samples	11
Total Samples	35

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43749]	16S rRNA gene sequence	LC014930		GenBank

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GC-content

64 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #43749]

Culture collection no.

BCC 73740, NBRC 110722

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Rhizobium puerariae sp. nov., an endophytic bacterium from the root nodules of the medicinal plant Pueraria candollei var. candollei.	Boonsnongcheep P, Prathanturarug S, Takahashi Y, Matsumoto A	Int J Syst Evol Microbiol	10.1099/ijsem.0.000863	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43749

Panitch Boonsnongcheep, Sompop Prathanturarug, Yoko Takahashi, Atsuko Matsumoto: Rhizobium puerariae sp. nov., an endophytic bacterium from the root nodules of the medicinal plant Pueraria candollei var. candollei. IJSEM 66: 1236 - 1241 2016 ( DOI 10.1099/ijsem.0.000863 , PubMed 26704271 )

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

* These data were automatically processed and therefore are not curated

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