Name: Leeuwenhoekiella nanhaiensis G18
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140891

Type strain:

Species: Leeuwenhoekiella nanhaiensis

Strain Designation: G18

Culture col. no.: CCTCC AB 2015204, KCTC 42729

NCBI tax ID(s): 1655491 (species)

Section

Leeuwenhoekiella nanhaiensis G18 is an aerobe, heterotroph, Gram-negative bacterium that forms circular colonies and was isolated from The deep South China Sea, depth of 2000 m.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
heterotroph
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Leeuwenhoekiella*
	Species	**Leeuwenhoekiella nanhaiensis**
	Full Scientific Name (LPSN)	Leeuwenhoekiella nanhaiensis Liu et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43723]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.995

[Ref.: #43723]	Cell length	1.4-4.1 µm

[Ref.: #43723]	Cell width	0.4-0.7 µm

[Ref.: #43723]	Cell shape	rod-shaped

[Ref.: #43723]	Motility	yes

[Ref.: #43723]	Flagellum arrangement	gliding

[Ref.: #43723]	Colony size	2-3 mm
[Ref.: #43723]	Colony color	Yellow
[Ref.: #43723]	Colony shape	circular
[Ref.: #43723]	Incubation period	3-4 days
[Ref.: #43723]	Cultivation medium used	MA agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43723]

Culture medium

Marine agar (MA)

[Ref.: #43723]

Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #43723]			growth	4-40 °C
[Ref.: #43723]			optimum	28-32 °C

[Ref.: #43723]

Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43723]			optimum	6.5-7.5
[Ref.: #43723]			growth	6-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43723]

Oxygen tolerance

aerobe

[Ref.: #43723]

Nutrition type

heterotroph

[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.995

[Ref.: #43723]

Halophily / tolerance level

slightly halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43723]		NaCl		growth	0-12	%(w/v)
[Ref.: #43723]		NaCl		optimum	3-4	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43723]	agar ChEBI	-	hydrolysis
[Ref.: #43723]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #43723]	casein	-	hydrolysis
[Ref.: #43723]	chitin ChEBI	-	hydrolysis
[Ref.: #43723]	citrate ChEBI	+	assimilation
[Ref.: #43723]	D-galactose ChEBI	-	builds acid from
[Ref.: #43723]	D-glucose ChEBI	+	assimilation
[Ref.: #43723]	D-glucose ChEBI	+	builds acid from
[Ref.: #43723]	D-mannose ChEBI	-	builds acid from
[Ref.: #43723]	D-mannose ChEBI	+	assimilation
[Ref.: #43723]	D-ribose ChEBI	-	builds acid from
[Ref.: #43723]	dna ChEBI	-	hydrolysis
[Ref.: #43723]	fructose ChEBI	-	builds acid from
[Ref.: #43723]	gelatin ChEBI	+	degradation
[Ref.: #43723]	glycerol 3-phosphate ChEBI	-	builds acid from
[Ref.: #43723]	L-arabinose ChEBI	+	assimilation
[Ref.: #43723]	L-arabinose ChEBI	+	builds acid from
[Ref.: #43723]	L-fucose ChEBI	-	builds acid from
[Ref.: #43723]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #43723]	lactose ChEBI	-	builds acid from
[Ref.: #43723]	lactose ChEBI	+	assimilation
[Ref.: #43723]	malonate ChEBI	-	builds acid from
[Ref.: #43723]	maltose ChEBI	-	builds acid from
[Ref.: #43723]	maltose ChEBI	+	assimilation
[Ref.: #43723]	mannitol ChEBI	-	builds acid from
[Ref.: #43723]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43723]	N-acetylglucosamine ChEBI	-	builds acid from
[Ref.: #43723]	nitrate ChEBI	-	reduction
[Ref.: #43723]	raffinose ChEBI	-	builds acid from
[Ref.: #43723]	sorbitol ChEBI	-	builds acid from
[Ref.: #43723]	starch ChEBI	+	degradation
[Ref.: #43723]	sucrose ChEBI	-	builds acid from
[Ref.: #43723]	sucrose ChEBI	+	assimilation
[Ref.: #43723]	tween 20 ChEBI	+	degradation
[Ref.: #43723]	tween 40 ChEBI	+	degradation
[Ref.: #43723]	tween 60 ChEBI	+	degradation
[Ref.: #43723]	tween 80 ChEBI	+	degradation
[Ref.: #43723]	urea ChEBI	-	hydrolysis
[Ref.: #43723]	xylose ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43723]	chloramphenicol ChEBI				yes	30	µg (disc)
[Ref.: #43723]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #43723]	penicillin ChEBI	yes	10	µg (disc)
[Ref.: #43723]	tetracycline ChEBI	yes	30	µg (disc)

	Metabolite	Production
[Ref.: #43723]	acetoin ChEBI	no
[Ref.: #43723]	hydrogen sulfide ChEBI	no
[Ref.: #43723]	indole ChEBI	no

	Physiological tests	Metabolite	Voges-Proskauer-test
[Ref.: #43723]		acetoin ChEBI	-

	Enzyme	Enzyme activity	EC number
[Ref.: #43723]	acid phosphatase	+	3.1.3.2
[Ref.: #43723]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43723]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43723]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43723]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43723]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43723]	alpha-mannosidase	+	3.2.1.24
[Ref.: #43723]	arginine dihydrolase	+	3.5.3.6
[Ref.: #43723]	beta-galactosidase	+	3.2.1.23
[Ref.: #43723]	beta-glucosidase	+	3.2.1.21
[Ref.: #43723]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43723]	catalase	+	1.11.1.6
[Ref.: #43723]	cystine arylamidase	+	3.4.11.3
[Ref.: #43723]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43723]	esterase (C 4)	+/-
[Ref.: #43723]	esterase Lipase (C 8)	+/-
[Ref.: #43723]	leucine arylamidase	+	3.4.11.1
[Ref.: #43723]	lipase (C 14)	-
[Ref.: #43723]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #43723]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43723]	trypsin	+	3.4.21.4
[Ref.: #43723]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43723]

Fatty acid	Percentage
C16:0 iso	22.4
C15:0 iso	19
C17:1 iso ω9c / C16:0 10-methyl	14.5
C11:0 iso	11.6
C11:0 iso 3OH	9.3
C14:0 iso	5.3
C16:1ω6c / C16:1ω7c	4.6
C17:0 iso	1.9
C12:0 iso	1.3
C15:0 anteiso	1.1

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43723]	Sample type/isolated from	The deep South China Sea, depth of 2000 m
[Ref.: #43723]	Geographic location (country and/or sea, region)	China Sea
[Ref.: #43723]	Geographic location	14.0000°/114.0000°
[Ref.: #43723]	Isolation date	2014-07-12
* marker position based on {}

Isolation sources categories

#Environmental

#Aquatic

#Marine

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43723]	16S rRNA gene sequence	KR809379		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Leeuwenhoekiella nanhaiensis G18	GCA_002744755	scaffold	GenBank	1655491 tax ID	*
[Ref.: #66792]	Leeuwenhoekiella nanhaiensis G18	2775506714	draft	JGI IMG/M	1655491 tax ID	*

[Ref.: #43723]

GC-content

35 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Leeuwenhoekiella nanhaiensis G18 NCBI: GCA_002744755.1
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Leeuwenhoekiella nanhaiensis G18 NCBI: GCA_002744755.1
[Ref.: #69480]	flagellated	no	95.578	no
[Ref.: #69480]	gram-positive	no	97.416	yes
[Ref.: #69480]	anaerobic	no	99.737	yes
[Ref.: #69480]	aerobic	yes	91.391	yes
[Ref.: #69480]	halophile	no	58.155	no
[Ref.: #69480]	spore-forming	no	96.588	no
[Ref.: #69480]	glucose-util	yes	84.553	no
[Ref.: #69480]	thermophile	no	99.375	yes
[Ref.: #69480]	motile	no	87.218	no
[Ref.: #69480]	glucose-ferment	no	86.97	no

Availability in culture collections External links

[Ref.: #43723]

Culture collection no.

CCTCC AB 2015204, KCTC 42729

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Leeuwenhoekiella nanhaiensis sp. nov., isolated from deep-sea water.	Liu Q, Li J, Wei B, Zhang X, Zhang L, Zhang Y, Fang J	Int J Syst Evol Microbiol	10.1099/ijsem.0.000883	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43723

Qianfeng Liu, Jiangtao Li, Bingbing Wei, Xiying Zhang, Li Zhang, Yuzhong Zhang, Jiasong Fang: Leeuwenhoekiella nanhaiensis sp. nov., isolated from deep-sea water. IJSEM 66: 1352 - 1357 2016 ( DOI 10.1099/ijsem.0.000883 , PubMed 26745974 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Leeuwenhoekiella nanhaiensis in external resources:
SILVA
BRENDA
PANGAEA
StrainInfo
GBIF