Name: Bacillus piscicola NR1-3-2
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140881

Type strain:

Species: Bacillus piscicola

Strain Designation: NR1-3-2

Culture col. no.: JCM 19598, LMG 28281, PCU 340, TISTR 2295

Strain history: S. Tanasupawat; Chulalongkorn Univ., Thailand; NR1-3-2.

NCBI tax ID(s): 1632684 (species)

Section

Bacillus piscicola NR1-3-2 is an obligate aerobe, spore-forming, Gram-positive bacterium that forms circular colonies and was isolated from Fish sauce collected from a factory.

colony-forming
Gram-positive
motile
rod-shaped
spore-forming
obligate aerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacillota*
	Class	*Bacilli*
	Order	*Caryophanales*
	Family	*Bacillaceae*
	Genus	*Bacillus*
	Species	**Bacillus piscicola**
	Full Scientific Name (LPSN)	Bacillus piscicola Daroonpunt et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43706]	Gram stain	positive

[Ref.: #43706]	Cell length	1.4-3.7 µm

[Ref.: #43706]	Cell width	0.64-0.81 µm

[Ref.: #43706]	Cell shape	rod-shaped

[Ref.: #43706]	Motility	yes

[Ref.: #43706]	Flagellum arrangement	peritrichous

[Ref.: #43706]	Colony size	1-2 mm
[Ref.: #43706]	Colony color	Cream
[Ref.: #43706]	Colony shape	circular
[Ref.: #43706]	Incubation period	3 days
[Ref.: #43706]	Cultivation medium used	Modofied JCM medium no. 377 agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43706]

Culture medium

Modofied JCM medium no. 377 agar

[Ref.: #43706]

Culture medium growth

		Temperature
[Ref.: #43706]	growth	21-48 °C
[Ref.: #43706]	optimum	37 °C
[Ref.: #67770]	growth	30 °C

[Ref.: #43706]	Temperature range	mesophilic
[Ref.: #67770]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43706]			optimum	7.5
[Ref.: #43706]			growth	5-10

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43706]

Oxygen tolerance

obligate aerobe

[Ref.: #43706]	Spore description	Ellipsoidal endospores at central-subterminal positions in non-swollen sporangia
[Ref.: #43706]	Type of spore	endospore
[Ref.: #43706]	Ability of spore formation	yes

[Ref.: #43706]

Halophily / tolerance level

slightly halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43706]		NaCl		growth	0-15	%(w/v)
[Ref.: #43706]		NaCl		optimum	1-3	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-7

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43706]	casein	-	hydrolysis
[Ref.: #43706]	cellobiose ChEBI	-	builds acid from
[Ref.: #43706]	D-fructose ChEBI	-	builds acid from
[Ref.: #43706]	D-galactose ChEBI	-	builds acid from
[Ref.: #43706]	D-glucose ChEBI	-	builds acid from
[Ref.: #43706]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43706]	D-mannose ChEBI	-	builds acid from
[Ref.: #43706]	D-ribose ChEBI	-	builds acid from
[Ref.: #43706]	D-sorbitol ChEBI	-	builds acid from
[Ref.: #43706]	D-xylose ChEBI	-	builds acid from
[Ref.: #43706]	gelatin ChEBI	-	hydrolysis
[Ref.: #43706]	glycerol ChEBI	-	builds acid from
[Ref.: #43706]	L-arabinose ChEBI	-	builds acid from
[Ref.: #43706]	L-arginine ChEBI	-	hydrolysis
[Ref.: #43706]	L-rhamnose ChEBI	-	builds acid from
[Ref.: #43706]	lactose ChEBI	-	builds acid from
[Ref.: #43706]	maltose ChEBI	-	builds acid from
[Ref.: #43706]	melezitose ChEBI	-	builds acid from
[Ref.: #43706]	melibiose ChEBI	-	builds acid from
[Ref.: #43706]	methyl D-glucoside ChEBI	-	builds acid from
[Ref.: #43706]	myo-inositol ChEBI	-	builds acid from
[Ref.: #43706]	nitrate ChEBI	-	reduction
[Ref.: #43706]	raffinose ChEBI	-	builds acid from
[Ref.: #43706]	starch ChEBI	-	hydrolysis
[Ref.: #43706]	sucrose ChEBI	-	builds acid from
[Ref.: #43706]	trehalose ChEBI	-	builds acid from
[Ref.: #43706]	tween 80 ChEBI	-	hydrolysis
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration
[Ref.: #43706]	amikacin ChEBI	yes	30	µg (disc)
[Ref.: #43706]	amoxicillin ChEBI	yes	30	µg (disc)
[Ref.: #43706]	cefepime ChEBI	yes	30	µg (disc)
[Ref.: #43706]	cefotaxime ChEBI	yes	30	µg (disc)
[Ref.: #43706]	cefoxitin ChEBI	yes	30	µg (disc)
[Ref.: #43706]	ceftriaxone ChEBI	yes	30	µg (disc)
[Ref.: #43706]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #43706]	clindamycin ChEBI	yes	2	µg (disc)
[Ref.: #43706]	ertapenem ChEBI	yes	10	µg (disc)
[Ref.: #43706]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #43706]	gentamicin ChEBI	yes	10	µg (disc)
[Ref.: #43706]	imipenem ChEBI	yes	10	µg (disc)
[Ref.: #43706]	kanamycin ChEBI	yes	30	µg (disc)
[Ref.: #43706]	levofloxacin ChEBI	yes	5	µg (disc)
[Ref.: #43706]	netilmycin ChEBI	yes	30	µg (disc)
[Ref.: #43706]	nitrofurantoin ChEBI	yes	300	µg (disc)
[Ref.: #43706]	penicillin g ChEBI	yes	20	Unit (disc)
[Ref.: #43706]	polymyxin b ChEBI	yes	100	Unit (disc)
[Ref.: #43706]	rifampicin ChEBI	yes	5	µg (disc)
[Ref.: #43706]	sulfamethoxazole ChEBI	yes	25	µg (disc)
[Ref.: #43706]	tazobactam ChEBI	yes	10	µg (disc)
[Ref.: #43706]	tetracycline ChEBI	yes	30	µg (disc)
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43706]	acid phosphatase	-	3.1.3.2
[Ref.: #43706]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43706]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43706]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43706]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43706]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43706]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43706]	beta-galactosidase	-	3.2.1.23
[Ref.: #43706]	beta-glucosidase	-	3.2.1.21
[Ref.: #43706]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43706]	catalase	+	1.11.1.6
[Ref.: #43706]	cystine arylamidase	-	3.4.11.3
[Ref.: #43706]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43706]	esterase (C 4)	+
[Ref.: #43706]	esterase Lipase (C 8)	+
[Ref.: #43706]	leucine arylamidase	+	3.4.11.1
[Ref.: #43706]	lipase (C 14)	-
[Ref.: #43706]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43706]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43706]	trypsin	-	3.4.21.4
[Ref.: #43706]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43706]	Sample type/isolated from	Fish sauce collected from a factory
[Ref.: #43706]	Geographic location (country and/or sea, region)	Chonburi Provicne
[Ref.: #43706]	Country	Thailand
[Ref.: #43706]	Country ISO 3 Code	THA
[Ref.: #43706]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Fish sauce (Nam-pla) from a factory
[Ref.: #67770]	Geographic location (country and/or sea, region)	Chonburi Province
[Ref.: #67770]	Country	Thailand
[Ref.: #67770]	Country ISO 3 Code	THA
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Engineered	#Food production	#Food
#Engineered	#Industrial	#Plant (Factory)

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43706]	16S rRNA gene sequence	LC036257		GenBank

[Ref.: #43706]

GC-content

44.2 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #43706]

Culture collection no.

JCM 19598, LMG 28281, PCU 340, TISTR 2295

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Bacillus piscicola sp. nov., isolated from Thai fish sauce (Nam-pla).	Daroonpunt R, Itoh T, Kudo T, Ohkuma M, Tanasupawat S	Int J Syst Evol Microbiol	10.1099/ijsem.0.000851	2015	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43706

Rungsima Daroonpunt, Takashi Itoh, Takuji Kudo, Moriya Ohkuma, Somboon Tanasupawat: Bacillus piscicola sp. nov., isolated from Thai fish sauce (Nam-pla). IJSEM 66: 1151 - 1155 2016 ( DOI 10.1099/ijsem.0.000851 , PubMed 26674773 )

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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