Name: Azospirillum agricola CC-HIH038
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140877

Type strain:

Species: Azospirillum agricola

Strain Designation: CC-HIH038

Culture col. no.: BCRC 80909, JCM 30827, CECT 9207

Strain history: C.-C. Young; Natl. Chung Hsing Univ., Taiwan; CC-HIH038.

NCBI tax ID(s): 1720247 (species)

Section

Azospirillum agricola CC-HIH038 is a facultative aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from Cultivated Soil.

colony-forming
Gram-negative
motile
spiral-shaped
facultative aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Pseudomonadota*
	Class	*Alphaproteobacteria*
	Order	*Rhodospirillales*
	Family	*Azospirillaceae*
	Genus	*Azospirillum*
	Species	**Azospirillum agricola**
	Full Scientific Name (LPSN)	Azospirillum agricola Lin et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43704]	Gram stain	negative

[Ref.: #43704]	Cell length	3.2-3.5 µm

[Ref.: #43704]	Cell width	1.3-1.7 µm

[Ref.: #43704]	Cell shape	spiral-shaped

[Ref.: #43704]	Motility	yes

[Ref.: #43704]	Flagellum arrangement	monotrichous, polar

[Ref.: #43704]	Colony size	1-2 mm
[Ref.: #43704]	Colony color	Pink
[Ref.: #43704]	Colony shape	circular
[Ref.: #43704]	Incubation period	2 days
[Ref.: #43704]	Cultivation medium used	Nutrient agar

Information on culture and growth conditions Culture and growth conditions

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

Nitrogen-free medium

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Culture medium growth

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Culture medium

Nutrient broth

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Culture medium growth

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Culture medium

Reasoner's 2A agar (R2A)

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Culture medium growth

	Temperatures	Kind of temperature		Temperature
[Ref.: #43704]			growth	20-37 °C
[Ref.: #67770]			growth	30 °C

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Temperature range

mesophilic

	pH	Kind of pH		pH
[Ref.: #43704]			growth	7-8

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43704]

Oxygen tolerance

facultative aerobe

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43704]		NaCl		growth	0-1	%(w/v)

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Observation

quinones: Q-10

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43704]	2-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #43704]	acetate ChEBI	+	carbon source
[Ref.: #43704]	beta-hydroxybutyrate ChEBI	+	carbon source
[Ref.: #43704]	cis-aconitate ChEBI	+	carbon source
[Ref.: #43704]	citrate ChEBI	+	carbon source
[Ref.: #43704]	D-fructose ChEBI	+	carbon source
[Ref.: #43704]	D-galactose ChEBI	+	carbon source
[Ref.: #43704]	D-gluconate ChEBI	+	carbon source
[Ref.: #43704]	D-glucose ChEBI	-	assimilation
[Ref.: #43704]	D-glucose 6-phosphate ChEBI	+	carbon source
[Ref.: #43704]	D-mannitol ChEBI	-	assimilation
[Ref.: #43704]	D-mannitol ChEBI	-	carbon source
[Ref.: #43704]	D-mannose ChEBI	+	assimilation
[Ref.: #43704]	D-sorbitol ChEBI	-	carbon source
[Ref.: #43704]	dl-alanine ChEBI	+	carbon source
[Ref.: #43704]	gelatin ChEBI	+	hydrolysis
[Ref.: #43704]	glucose 1-phosphate ChEBI	+	carbon source
[Ref.: #43704]	glucuronamide ChEBI	+	carbon source
[Ref.: #43704]	glycerol ChEBI	+	carbon source
[Ref.: #43704]	L-alaninamide ChEBI	+	carbon source
[Ref.: #43704]	L-arabinose ChEBI	+	assimilation
[Ref.: #43704]	L-arabinose ChEBI	+	carbon source
[Ref.: #43704]	L-asparagine ChEBI	+	carbon source
[Ref.: #43704]	L-glutamate ChEBI	+	carbon source
[Ref.: #43704]	L-histidine ChEBI	+	carbon source
[Ref.: #43704]	L-leucine ChEBI	+	carbon source
[Ref.: #43704]	L-ornithine ChEBI	+	carbon source
[Ref.: #43704]	L-proline ChEBI	+	carbon source
[Ref.: #43704]	L-serine ChEBI	+	carbon source
[Ref.: #43704]	L-threonine ChEBI	+	carbon source
[Ref.: #43704]	lactate ChEBI	+	carbon source
[Ref.: #43704]	malate ChEBI	+	assimilation
[Ref.: #43704]	methyl pyruvate ChEBI	+	carbon source
[Ref.: #43704]	monomethyl succinate ChEBI	+	carbon source
[Ref.: #43704]	nitrate ChEBI	+	reduction
[Ref.: #43704]	potassium gluconate ChEBI	-	assimilation
[Ref.: #43704]	quinate ChEBI	+	carbon source
[Ref.: #43704]	succinate ChEBI	+	carbon source
[Ref.: #43704]	tween 40 ChEBI	+	carbon source
[Ref.: #43704]	tween 80 ChEBI	-	carbon source
[Ref.: #43704]	urocanic acid ChEBI	+	carbon source
	Only first 10 entries are displayed. Click here to see all.

	Enzyme	Enzyme activity	EC number
[Ref.: #43704]	acid phosphatase	+	3.1.3.2
[Ref.: #43704]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43704]	beta-galactosidase	-	3.2.1.23
[Ref.: #43704]	beta-glucosidase	+	3.2.1.21
[Ref.: #43704]	catalase	+	1.11.1.6
[Ref.: #43704]	cystine arylamidase	+	3.4.11.3
[Ref.: #43704]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43704]	esterase (C 4)	+
[Ref.: #43704]	esterase Lipase (C 8)	+
[Ref.: #43704]	leucine arylamidase	+	3.4.11.1
[Ref.: #43704]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43704]	trypsin	-	3.4.21.4
[Ref.: #43704]	urease	+	3.5.1.5
[Ref.: #43704]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43704]

Fatty acid	Percentage
C16:1ω7c / C15:0 iso 2OH	26.9
C16:0	22.5
iso-C16:1 / C14:0 3-OH	13.6
C18:1ω7c	12.4
C12:0	9.6
C17:0cyclo	5.8

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43704]	Sample type/isolated from	Cultivated Soil
[Ref.: #43704]	Geographic location (country and/or sea, region)	Wufeng District, Taichung City
[Ref.: #43704]	Country	Taiwan
[Ref.: #43704]	Country ISO 3 Code	TWN
[Ref.: #43704]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Cultivated soil
[Ref.: #67770]	Country	Taiwan
[Ref.: #67770]	Country ISO 3 Code	TWN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Soil
#Engineered	#Agriculture	-

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43704]	16S rRNA gene sequence	KR296799		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #66792]	Azospirillum agricola CC-HIH038	GCA_017876095	contig	GenBank	1720247 tax ID	*
[Ref.: #66792]	Azospirillum agricola CC-HIH038	2918249483	draft	JGI IMG/M	1720247 tax ID	*
[Ref.: #66792]	Azospirillum agricola CC-HIH038	2928819513	draft	JGI IMG/M	1720247 tax ID	*

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GC-content

68.8 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

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Culture collection no.

BCRC 80909, JCM 30827, CECT 9207

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Azospirillum thermophilum sp. nov., isolated from a hot spring.	Zhao ZL, Ming H, Ding CL, Ji WL, Cheng LJ, Niu MM, Zhang YM, Zhang LY, Meng XL, Nie GX	Int J Syst Evol Microbiol	10.1099/ijsem.0.003788	2020	*
Phylogeny	Azospirillum griseum sp. nov., isolated from lakewater.	Yang Y, Zhang R, Feng J, Wang C, Chen J	Int J Syst Evol Microbiol	10.1099/ijsem.0.003460	2019	*
Phylogeny	Azospirillum agricola sp. nov., a nitrogen-fixing species isolated from cultivated soil.	Lin SY, Liu YC, Hameed A, Hsu YH, Huang HI, Lai WA, Young CC	Int J Syst Evol Microbiol	10.1099/ijsem.0.000904	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43704

Shih-Yao Lin, You-Cheng Liu, Asif Hameed, Yi-Han Hsu, Hsin-I Huang, Wei-An Lai, Chiu-Chung Young: Azospirillum agricola sp. nov., a nitrogen-fixing species isolated from cultivated soil. IJSEM 66: 1453 - 1458 2016 ( DOI 10.1099/ijsem.0.000904 , PubMed 26786719 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
* These data were automatically processed and therefore are not curated

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