Name: Sphaerimonospora cavernae N74
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140868

Type strain:

Species: Sphaerimonospora cavernae

Strain Designation: N74

Culture col. no.: BCC 77604, NBRC 111481, DSM 111736

NCBI tax ID(s): 1740611 (species)

SI-ID 397818

DSM 111736

Section

Sphaerimonospora cavernae N74 is a spore-forming, Gram-positive bacterium that was isolated from Soil collected from a limestone cave.

Gram-positive
spore-forming
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Actinomycetota*
	Class	*Actinomycetes*
	Order	*Streptosporangiales*
	Family	*Streptosporangiaceae*
	Genus	*Sphaerimonospora*
	Species	**Sphaerimonospora cavernae**
	Full Scientific Name (LPSN)	Sphaerimonospora cavernae Mingma et al. 2016

Information on morphological and physiological properties Morphology

[Ref.: #43691]	Gram stain	positive

[Ref.: #43691]	Motility	no

Information on culture and growth conditions Culture and growth conditions

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Culture medium

ISP medium 2

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Culture medium growth

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Culture medium

Nutrient agar (NA)

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Culture medium growth

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Culture medium

GYM STREPTOMYCES MEDIUM (DSMZ Medium 65)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

N-Z-AMINE-MEDIUM (DSMZ Medium 554)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

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Culture medium

ISP2 MEDIUM (DSMZ Medium 987)

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Culture medium growth

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Culture medium link

Medium recipe at MediaDive

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Culture medium composition

expand / minimize

		Temperature
[Ref.: #43691]	growth	22-36 °C
[Ref.: #43691]	optimum	26-34 °C
[Ref.: #67658]	growth	28 °C

[Ref.: #43691]	Temperature range	mesophilic
[Ref.: #67658]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43691]			growth	6-7

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43691]	Spore description	spherical, single spores (diamete 1.5-1.7 µm) with a smooth surface on aerial mycelium
[Ref.: #43691]	Type of spore	spore
[Ref.: #43691]	Ability of spore formation	yes

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43691]		NaCl		maximum	2	%(w/v)

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43691]	(-)-D-fructose ChEBI	-	builds acid from
[Ref.: #43691]	(-)-D-fructose ChEBI	-	carbon source
[Ref.: #43691]	(-)-D-fructose ChEBI	-	degradation
[Ref.: #43691]	(-)-D-rhamnose	-	degradation
[Ref.: #43691]	adenine ChEBI	-	hydrolysis
[Ref.: #43691]	beta-lactose ChEBI	-	builds acid from
[Ref.: #43691]	beta-lactose ChEBI	-	degradation
[Ref.: #43691]	casein	+	hydrolysis
[Ref.: #43691]	cellobiose ChEBI	+	builds acid from
[Ref.: #43691]	cellobiose ChEBI	+	degradation
[Ref.: #43691]	cellulose ChEBI	-	hydrolysis
[Ref.: #43691]	D-galactose ChEBI	-	carbon source
[Ref.: #43691]	D-galactose ChEBI	-	degradation
[Ref.: #43691]	D-mannitol ChEBI	-	builds acid from
[Ref.: #43691]	D-mannitol ChEBI	-	carbon source
[Ref.: #43691]	D-mannitol ChEBI	-	degradation
[Ref.: #43691]	D-mannose ChEBI	+	carbon source
[Ref.: #43691]	D-mannose ChEBI	+	degradation
[Ref.: #43691]	gelatin ChEBI	+	hydrolysis
[Ref.: #43691]	glucose ChEBI	+	builds acid from
[Ref.: #43691]	glucose ChEBI	+	degradation
[Ref.: #43691]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #43691]	L-arabinose ChEBI	-	carbon source
[Ref.: #43691]	L-arabinose ChEBI	-	degradation
[Ref.: #43691]	maltose ChEBI	-	builds acid from
[Ref.: #43691]	maltose ChEBI	+	degradation
[Ref.: #43691]	melibiose ChEBI	-	degradation
[Ref.: #43691]	myo-inositol ChEBI	-	degradation
[Ref.: #43691]	nitrate ChEBI	+	reduction
[Ref.: #43691]	raffinose ChEBI	-	degradation
[Ref.: #43691]	ribose ChEBI	-	degradation
[Ref.: #43691]	sorbose ChEBI	-	degradation
[Ref.: #43691]	starch ChEBI	-	hydrolysis
[Ref.: #43691]	sucrose ChEBI	-	degradation
[Ref.: #43691]	tween 80 ChEBI	+	hydrolysis
[Ref.: #43691]	xanthine ChEBI	-	hydrolysis
[Ref.: #43691]	xylitol ChEBI	-	degradation
[Ref.: #43691]	xylose ChEBI	+	builds acid from
[Ref.: #43691]	xylose ChEBI	+	degradation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite production	Metabolite	Production
[Ref.: #43691]		hydrogen sulfide ChEBI	no
[Ref.: #43691]		melanin ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43691]	acid phosphatase	+/-	3.1.3.2
[Ref.: #43691]	alkaline phosphatase	-	3.1.3.1
[Ref.: #43691]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43691]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43691]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43691]	alpha-glucosidase	+	3.2.1.20
[Ref.: #43691]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43691]	beta-galactosidase	-	3.2.1.23
[Ref.: #43691]	beta-glucosidase	+	3.2.1.21
[Ref.: #43691]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43691]	catalase	+	1.11.1.6
[Ref.: #43691]	cystine aminopeptidase	+	3.4.11.3
[Ref.: #43691]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43691]	esterase	+
[Ref.: #43691]	esterase Lipase (C 8)	+
[Ref.: #43691]	leucine aminopeptidase	+	3.4.1.1
[Ref.: #43691]	lipase (C 14)	-
[Ref.: #43691]	N-acetyl-beta-glucosaminidase	+	3.2.1.52
[Ref.: #43691]	naphthol-AS-BI-phosphohydrolase	-
[Ref.: #43691]	phosphoamidase	-	3.9.1.1
[Ref.: #43691]	trypsin	+	3.4.21.4
[Ref.: #43691]	valine aminopeptidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

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Fatty acid	Percentage
C15:0 anteiso	44.3
C14:0 iso	18
C16:0 iso	7
C14:0	6.1
C15:0 iso	4.3
C16:0	3.9
C15:1 iso h / C13:0 3OH	2.7
C16:1ω7c / C16:1ω6c	1.7
C18:0	1.6
C13:1at12-13	1.5
C12:0	1
C15:1ω8c	0.7
C20:2ω6,9c	0.6
C17:0 anteiso	0.6
C20:4ω6,9,12,15c	0.5
C15:1ω6c	0.5

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43691]	Sample type/isolated from	Soil collected from a limestone cave
[Ref.: #43691]	Geographic location (country and/or sea, region)	Nakhonsawan Province
[Ref.: #43691]	Country	Thailand
[Ref.: #43691]	Country ISO 3 Code	THA
[Ref.: #43691]	Continent	Asia

[Ref.: #67658]	Sample type/isolated from	cave soil
[Ref.: #67658]	Geographic location (country and/or sea, region)	Nakhon Sawan
[Ref.: #67658]	Country	Thailand
[Ref.: #67658]	Country ISO 3 Code	THA
[Ref.: #67658]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Terrestrial	#Geologic
#Environmental	#Terrestrial	#Soil

What are isolation sources categories?

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Global distribution of 16S sequence LC096135 (>99% sequence identity) for Sphaerimonospora from Microbeatlas

Aquatic Samples	6
Soil Samples	63
Animal Samples	16
Plant Samples	12
Total Samples	97

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

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Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #67658]		>50_DSM_111736_900R		ENA
[Ref.: #43691]	16S rRNA gene sequence	LC096135		GenBank

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GC-content

70.7 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

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Culture collection no.

BCC 77604, NBRC 111481, DSM 111736

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SI-ID 397818

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Sphaerimonospora triticiradicis sp. nov., a novel actinomycete isolated from a root of wheat (Triticum aestivum L.).	Han C, Hu J, Zhao J, Tian Y, Jiang S, Guo X, Xiang W, Wang X	Antonie Van Leeuwenhoek	10.1007/s10482-018-1170-1	2018	*
Phylogeny	Proposal of Sphaerimonospora cavernae gen. nov., sp. nov. and transfer of Microbispora mesophila (Zhang et al., 1998) to Sphaerimonospora mesophila comb. nov. and Microbispora thailandensis (Duangmal et al., 2012) to Sphaerimonospora thailandensis comb. nov.	Mingma R, Duangmal K, Take A, Inahashi Y, O Mura S, Takahashi YK, Matsumoto A	Int J Syst Evol Microbiol	10.1099/ijsem.0.000935	2016	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43691

Ratchanee Mingma, Kannika Duangmal, Akira Také, Yuki Inahashi, Satoshi Omura, Yoko Takahashi, Atsuko Matsumoto: Proposal of Sphaerimonospora cavernae gen. nov., sp. nov. and transfer of Microbispora mesophila (Zhang et al., 1998) to Sphaerimonospora mesophila comb. nov. and Microbispora thailandensis (Duangmal et al., 2012) to Sphaerimonospora thailandensis comb. nov.. IJSEM 66: 1735 - 1744 2016 ( DOI 10.1099/ijsem.0.000935 , PubMed 26822211 )

#67658

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 111736

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96803

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID397818.1 )

* These data were automatically processed and therefore are not curated

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