Name: Anaerotignum aminivorans SH021
Creator: BacDive
License: https://creativecommons.org/licenses/by/4.0/

Strain identifier

BacDive ID: 140747

Type strain:

Species: Anaerotignum aminivorans

Strain Designation: SH021

Culture col. no.: JCM 31556, DSM 103575, KCTC 15705

Strain history: A. Ueki; Fac. of Agric., Yamagata Univ., Japan; SH021.

NCBI tax ID(s): 433287 (species)

SI-ID 406740

DSM 103575

Special Collections

Section

Anaerotignum aminivorans SH021 is an obligate anaerobe, chemoorganotroph, Gram-positive bacterium that forms circular colonies and was isolated from a methanogenic reactor treating waste collected from cattle farms.

colony-forming
Gram-positive
motile
rod-shaped
chemoorganotroph
obligate anaerobe
16S sequence
Bacteria

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	09-12-2024 (DD-MM-YYYY)
	Domain	*Bacteria*
	Phylum	*Bacillota*
	Class	*Clostridia*
	Order	*Eubacteriales*
	Family	*Lachnospiraceae*
	Genus	*Anaerotignum*
	Species	**Anaerotignum aminivorans**
	Full Scientific Name (LPSN)	Anaerotignum aminivorans Ueki et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43524]	Gram stain	positive

[Ref.: #43524]	Cell length	2.5-8 µm

[Ref.: #43524]	Cell width	0.7 µm

[Ref.: #43524]	Cell shape	rod-shaped

[Ref.: #43524]	Motility	yes

[Ref.: #43524]	Flagellum arrangement	peritrichous

[Ref.: #43524]	Colony size	0.5-0.7 mm
[Ref.: #43524]	Colony color	greyish white
[Ref.: #43524]	Colony shape	circular
[Ref.: #43524]	Incubation period	2 days
[Ref.: #43524]	Cultivation medium used	PY4S agar

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43524]

Culture medium

PY4S agar

[Ref.: #43524]

Culture medium growth

[Ref.: #64905]

Culture medium

PY + X MEDIUM (N2/CO2) (DSMZ Medium 104c)

[Ref.: #64905]

Culture medium growth

[Ref.: #64905]

Culture medium link

Medium recipe at MediaDive

[Ref.: #64905]

Culture medium composition

expand / minimize

		Temperature
[Ref.: #43524]	growth	15-40 °C
[Ref.: #43524]	optimum	35 °C
[Ref.: #64905]	growth	30 °C
[Ref.: #67770]	growth	30 °C

	pH	Kind of pH		pH
[Ref.: #43524]			optimum	6.1-7.7
[Ref.: #43524]			growth	6.1-8.2

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43524]

Oxygen tolerance

obligate anaerobe

[Ref.: #43524]

Nutrition type

chemoorganotroph

[Ref.: #43524]

Halophily / tolerance level

non-halophilic

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43524]		NaCl		growth	0-20	g/L
[Ref.: #43524]		NaCl		optimum	0	g/L

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43524]	2-aminobutyrate ChEBI	-	assimilation
[Ref.: #43524]	acrylate ChEBI	-	assimilation
[Ref.: #43524]	amygdalin ChEBI	-	assimilation
[Ref.: #43524]	arabinose ChEBI	-	assimilation
[Ref.: #43524]	carboxymethylcellulose ChEBI	-	assimilation
[Ref.: #43524]	casamino acids	+/-	assimilation
[Ref.: #43524]	cellobiose ChEBI	-	assimilation
[Ref.: #43524]	cellulose ChEBI	-	assimilation
[Ref.: #43524]	crotonate ChEBI	-	assimilation
[Ref.: #43524]	dextrin ChEBI	-	assimilation
[Ref.: #43524]	esculin ChEBI	-	assimilation
[Ref.: #43524]	esculin ChEBI	+	hydrolysis
[Ref.: #43524]	ethanol ChEBI	-	assimilation
[Ref.: #43524]	fructose ChEBI	-	assimilation
[Ref.: #43524]	fumarate ChEBI	-	assimilation
[Ref.: #43524]	galactose ChEBI	-	assimilation
[Ref.: #43524]	gelatin ChEBI	-	hydrolysis
[Ref.: #43524]	glucose ChEBI	-	assimilation
[Ref.: #43524]	glycerol ChEBI	-	assimilation
[Ref.: #43524]	glycine ChEBI	-	assimilation
[Ref.: #43524]	glycogen ChEBI	-	assimilation
[Ref.: #43524]	inulin ChEBI	-	assimilation
[Ref.: #43524]	L-alanine ChEBI	+	assimilation
[Ref.: #43524]	L-arginine ChEBI	-	assimilation
[Ref.: #43524]	L-asparagine ChEBI	-	assimilation
[Ref.: #43524]	L-aspartate ChEBI	-	assimilation
[Ref.: #43524]	L-glutamate ChEBI	-	assimilation
[Ref.: #43524]	L-glutamine ChEBI	-	assimilation
[Ref.: #43524]	L-histidine ChEBI	-	assimilation
[Ref.: #43524]	L-isoleucine ChEBI	+/-	assimilation
[Ref.: #43524]	L-leucine ChEBI	+/-	assimilation
[Ref.: #43524]	L-lysine ChEBI	-	assimilation
[Ref.: #43524]	L-methionine ChEBI	-	assimilation
[Ref.: #43524]	L-phenylalanine ChEBI	-	assimilation
[Ref.: #43524]	L-proline ChEBI	-	assimilation
[Ref.: #43524]	L-serine ChEBI	+	assimilation
[Ref.: #43524]	L-threonine ChEBI	+	assimilation
[Ref.: #43524]	L-tryptophan ChEBI	-	assimilation
[Ref.: #43524]	L-valine ChEBI	+/-	assimilation
[Ref.: #43524]	lactate ChEBI	+	assimilation
[Ref.: #43524]	lactose ChEBI	-	assimilation
[Ref.: #43524]	malate ChEBI	-	assimilation
[Ref.: #43524]	maltose ChEBI	-	assimilation
[Ref.: #43524]	mannitol ChEBI	-	assimilation
[Ref.: #43524]	mannose ChEBI	-	assimilation
[Ref.: #43524]	melezitose ChEBI	-	assimilation
[Ref.: #43524]	melibiose ChEBI	-	assimilation
[Ref.: #43524]	myo-inositol ChEBI	-	assimilation
[Ref.: #43524]	nitrate ChEBI	-	reduction
[Ref.: #43524]	pectin ChEBI	-	assimilation
[Ref.: #43524]	pyruvate ChEBI	+	assimilation
[Ref.: #43524]	raffinose ChEBI	-	assimilation
[Ref.: #43524]	rhamnose ChEBI	-	assimilation
[Ref.: #43524]	ribose ChEBI	-	assimilation
[Ref.: #43524]	salicin ChEBI	-	assimilation
[Ref.: #43524]	sorbitol ChEBI	-	assimilation
[Ref.: #43524]	starch ChEBI	-	assimilation
[Ref.: #43524]	starch ChEBI	-	hydrolysis
[Ref.: #43524]	succinate ChEBI	-	assimilation
[Ref.: #43524]	sucrose ChEBI	-	assimilation
[Ref.: #43524]	trehalose ChEBI	-	assimilation
[Ref.: #43524]	xylan ChEBI	-	assimilation
[Ref.: #43524]	xylose ChEBI	-	assimilation
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Production
[Ref.: #43524]	acetate ChEBI	yes
[Ref.: #43524]	butyrate ChEBI	yes
[Ref.: #43524]	hydrogen sulfide ChEBI	yes
[Ref.: #43524]	indole ChEBI	no
[Ref.: #43524]	isobutyrate ChEBI	yes
[Ref.: #43524]	isovalerate ChEBI	yes
[Ref.: #43524]	propionate ChEBI	yes

	Enzyme	Enzyme activity	EC number
[Ref.: #43524]	catalase	-	1.11.1.6
[Ref.: #43524]	cytochrome oxidase	-	1.9.3.1
[Ref.: #43524]	lecithinase	-
[Ref.: #43524]	lipase (C 14)	-

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43524]	Sample type/isolated from	a methanogenic reactor treating waste collected from cattle farms
[Ref.: #43524]	Geographic location (country and/or sea, region)	Betsukai-machi, Hokkaido
[Ref.: #43524]	Country	Japan
[Ref.: #43524]	Country ISO 3 Code	JPN
[Ref.: #43524]	Continent	Asia

[Ref.: #64905]	Sample type/isolated from	methanogenic reactor treating waste from cattlefarms
[Ref.: #64905]	Geographic location (country and/or sea, region)	Hokkaido
[Ref.: #64905]	Country	Japan
[Ref.: #64905]	Country ISO 3 Code	JPN
[Ref.: #64905]	Continent	Asia

[Ref.: #67770]	Sample type/isolated from	Methanogenic reactor treating waste from cattle farms
[Ref.: #67770]	Country	Japan
[Ref.: #67770]	Country ISO 3 Code	JPN
[Ref.: #67770]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Condition	#Anoxic (anaerobic)	-
#Engineered	#Bioreactor	-
#Host	#Mammals	#Bovinae (Cow, Cattle)
#Host Body Product	#Gastrointestinal tract	#Feces (Stool)

What are isolation sources categories?

[Ref.: #69479]

Global distribution of 16S sequence AB298756 (>99% sequence identity) for Anaerotignum aminivorans subclade from Microbeatlas

Aquatic Samples	71
Soil Samples	16
Animal Samples	477
Plant Samples	33
Total Samples	597

Information on possible application of the strain and its possible interaction with e.g. potential hosts Safety information

[Ref.: #64905]

Biosafety level

Risk group (German classification)
According to TRBA 466

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #64905]	Anaerotignum aminivorans gene for 16S rRNA, partial sequence	AB298756	1471	GenBank ENA	433287 tax ID

[Ref.: #64905]

GC-content

38.2 mol%

high performance liquid chromatography (HPLC)

Availability in culture collections External links

[Ref.: #64905]

Culture collection no.

JCM 31556, DSM 103575, KCTC 15705

[Ref.: #96784]

SI-ID 406740

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Anaerotignum faecicola sp. nov., isolated from human faeces.	Choi SH, Kim JS, Park JE, Lee KC, Eom MK, Oh BS, Yu SY, Kang SW, Han KI, Suh MK, Lee DH, Yoon H, Kim BY, Lee JH, Lee JH, Lee JS, Park SH	J Microbiol	10.1007/s12275-019-9268-3	2019	*
Phylogeny	Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum.	Ueki A, Goto K, Ohtaki Y, Kaku N, Ueki K	Int J Syst Evol Microbiol	10.1099/ijsem.0.002268	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43524

Atsuko Ueki, Kazushi Goto, Yoshimi Ohtaki, Nobuo Kaku, Katsuji Ueki: Description of Anaerotignum aminivorans gen. nov., sp. nov., a strictly anaerobic, amino-acid-decomposing bacterium isolated from a methanogenic reactor, and reclassification of Clostridium propionicum, Clostridium neopropionicum and Clostridium lactatifermentans as species of the genus Anaerotignum. IJSEM 67: 4146 - 4153 2017 ( DOI 10.1099/ijsem.0.002268 , PubMed 28905695 )

#64905

Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 103575

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;

#69479

João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .

#96784

Reimer, L.C., Lissin, A.,Schober, I., Witte,J.F., Podstawka, A., Lüken, H., Bunk, B.,Overmann, J.: StrainInfo: A central database for resolving microbial strain identifiers . ( DOI 10.60712/SI-ID406740.1 )

* These data were automatically processed and therefore are not curated

Change proposal

You found an error in BacDive? Please tell us about it!

Note that changes will be reviewed and judged. If your changes are legitimate, changes will occur within the next BacDive update. Only proposed changes supported by the according reference will be reviewed. The BacDive team reserves the right to reject proposed changes.

Search for species Anaerotignum aminivorans in external resources:
SILVA
BRENDA
PANGAEA
GBIF