Name: Winogradskyella flava SFD31
Creator: BacDive
License: http://creativecommons.org/licenses/by-nc/4.0/

Strain identifier

BacDive ID: 140712

Type strain:

Species: Winogradskyella flava

Strain Designation: SFD31

Culture col. no.: KCTC 52348, JCM 31798

Strain history: <- Chi Nam Seong, Sunchon National Univ.

NCBI tax ID(s): 1884876 (species)

Section

Winogradskyella flava SFD31 is an aerobe, Gram-negative, motile bacterium that forms circular colonies and was isolated from brown alga, Sargassum fulvellum, collected from a natural seaweed bed in the South Sea.

colony-forming
Gram-negative
motile
rod-shaped
aerobe
16S sequence
Bacteria
genome sequence

Information on the name and the taxonomic classification. Name and taxonomic classification

	Last LPSN update	14-12-2023 (DD-MM-YYYY)
	Domain	"Bacteria"
	Phylum	*Bacteroidota*
	Class	*Flavobacteriia*
	Order	*Flavobacteriales*
	Family	*Flavobacteriaceae*
	Genus	*Winogradskyella*
	Species	**Winogradskyella flava**
	Full Scientific Name (LPSN)	Winogradskyella flava Lee et al. 2017

Information on morphological and physiological properties Morphology

[Ref.: #43487]	Gram stain	negative
[Ref.: #69480]	Gram stain	negative Confidence in %99.992

[Ref.: #43487]	Cell length	0.8-1.4 µm

[Ref.: #43487]	Cell width	0.4-0.6 µm

[Ref.: #43487]	Cell shape	rod-shaped

[Ref.: #43487]	Motility	yes

[Ref.: #43487]	Flagellum arrangement	gliding

[Ref.: #43487]	Colony size	0.5-1 mm
[Ref.: #43487]	Colony color	yellow
[Ref.: #43487]	Colony shape	circular
[Ref.: #43487]	Incubation period	5 days
[Ref.: #43487]	Cultivation medium used	MA agar

[Ref.: #43487]	Pigment production	no
[Ref.: #43487]	Pigment name	Flexirubin-type

Information on culture and growth conditions Culture and growth conditions

[Ref.: #43487]

Culture medium

Marine agar (MA)

[Ref.: #43487]

Culture medium growth

		Temperature
[Ref.: #43487]	growth	4-35 °C
[Ref.: #43487]	optimum	20-30 °C
[Ref.: #67770]	growth	25 °C
[Ref.: #67771]	growth	25 °C

[Ref.: #67770]	Temperature range	mesophilic
[Ref.: #67771]	Temperature range	mesophilic

	pH	Kind of pH		pH
[Ref.: #43487]			optimum	8
[Ref.: #43487]			growth	7-9

Information on physiology and metabolism Physiology and metabolism

[Ref.: #43487]	Oxygen tolerance	aerobe
[Ref.: #67771]	Oxygen tolerance	aerobe

[Ref.: #43487]	Ability of spore formation	no
[Ref.: #69481]	Ability of spore formation	no Confidence in %100
[Ref.: #69480]	Ability of spore formation	no Confidence in %99.984

	Halophily	Salt	Tested relation		Salt conc.
[Ref.: #43487]		NaCl		growth	2-3	%(w/v)
[Ref.: #43487]		NaCl		optimum	2	%(w/v)

[Ref.: #67770]

Observation

quinones: MK-6

	Metabolite	Utilization activity	Kind of utilization tested
[Ref.: #43487]	adipate ChEBI	-	assimilation
[Ref.: #43487]	arabinose ChEBI	-	assimilation
[Ref.: #43487]	arginine ChEBI	-	hydrolysis
[Ref.: #43487]	carboxymethylcellulose ChEBI	-	hydrolysis
[Ref.: #43487]	casein	-	hydrolysis
[Ref.: #43487]	chitin ChEBI	-	hydrolysis
[Ref.: #43487]	decanoate ChEBI	-	assimilation
[Ref.: #43487]	dna ChEBI	-	hydrolysis
[Ref.: #43487]	esculin ChEBI	+	hydrolysis
[Ref.: #43487]	fructose ChEBI	-	builds acid from
[Ref.: #43487]	galactose ChEBI	-	builds acid from
[Ref.: #43487]	gelatin ChEBI	+	hydrolysis
[Ref.: #43487]	glucose ChEBI	-	assimilation
[Ref.: #43487]	glucose ChEBI	-	builds acid from
[Ref.: #43487]	hypoxanthine ChEBI	-	hydrolysis
[Ref.: #43487]	lactose ChEBI	-	builds acid from
[Ref.: #43487]	malate ChEBI	-	assimilation
[Ref.: #43487]	maltose ChEBI	-	assimilation
[Ref.: #43487]	maltose ChEBI	-	builds acid from
[Ref.: #43487]	mannitol ChEBI	-	assimilation
[Ref.: #43487]	mannitol ChEBI	-	builds acid from
[Ref.: #43487]	mannose ChEBI	-	assimilation
[Ref.: #43487]	mannose ChEBI	-	builds acid from
[Ref.: #43487]	N-acetylglucosamine ChEBI	-	assimilation
[Ref.: #43487]	nitrate ChEBI	-	reduction
[Ref.: #43487]	phenylacetate ChEBI	-	assimilation
[Ref.: #43487]	potassium gluconate ChEBI	-	assimilation
[Ref.: #43487]	rhamnose ChEBI	-	builds acid from
[Ref.: #43487]	sodium citrate ChEBI	-	assimilation
[Ref.: #43487]	starch ChEBI	-	hydrolysis
[Ref.: #43487]	sucrose ChEBI	-	builds acid from
[Ref.: #43487]	trehalose ChEBI	-	builds acid from
[Ref.: #43487]	tween 20 ChEBI	-	hydrolysis
[Ref.: #43487]	tween 80 ChEBI	+	hydrolysis
[Ref.: #43487]	urea ChEBI	-	hydrolysis
[Ref.: #43487]	xanthine ChEBI	-	hydrolysis
[Ref.: #43487]	xylan ChEBI	-	hydrolysis
[Ref.: #43487]	xylose ChEBI	-	builds acid from
	Only first 10 entries are displayed. Click here to see all.

	Metabolite	Antibiotic sensitivity	Concentration		Antibiotic resistance	Concentration
[Ref.: #43487]	amikacin ChEBI				yes	30	µg (disc)
[Ref.: #43487]	ampicillin ChEBI	yes	10	µg (disc)
[Ref.: #43487]	chloramphenicol ChEBI	yes	30	µg (disc)
[Ref.: #43487]	erythromycin ChEBI	yes	15	µg (disc)
[Ref.: #43487]	gentamicin ChEBI				yes	10	µg (disc)
[Ref.: #43487]	kanamycin ChEBI				yes	30	µg (disc)
[Ref.: #43487]	nalidixic acid ChEBI				yes	30	µg (disc)
[Ref.: #43487]	penicillin ChEBI				yes	10	Unit (disc)
[Ref.: #43487]	streptomycin ChEBI				yes	10	µg (disc)
[Ref.: #43487]	vancomycin ChEBI	yes	30	µg (disc)

	Metabolite production	Metabolite	Production
[Ref.: #43487]		hydrogen sulfide ChEBI	no
[Ref.: #43487]		indole ChEBI	no

	Enzyme	Enzyme activity	EC number
[Ref.: #43487]	acid phosphatase	+	3.1.3.2
[Ref.: #43487]	alkaline phosphatase	+	3.1.3.1
[Ref.: #43487]	alpha-chymotrypsin	+	3.4.21.1
[Ref.: #43487]	alpha-fucosidase	-	3.2.1.51
[Ref.: #43487]	alpha-galactosidase	-	3.2.1.22
[Ref.: #43487]	alpha-glucosidase	-	3.2.1.20
[Ref.: #43487]	alpha-mannosidase	-	3.2.1.24
[Ref.: #43487]	beta-galactosidase	-	3.2.1.23
[Ref.: #43487]	beta-glucosidase	+	3.2.1.21
[Ref.: #43487]	beta-glucuronidase	-	3.2.1.31
[Ref.: #43487]	catalase	+	1.11.1.6
[Ref.: #43487]	cystine arylamidase	-	3.4.11.3
[Ref.: #43487]	cytochrome oxidase	+	1.9.3.1
[Ref.: #43487]	esterase (C 4)	+
[Ref.: #43487]	esterase Lipase (C 8)	+
[Ref.: #43487]	leucine arylamidase	+	3.4.11.1
[Ref.: #43487]	lipase (C 14)	-
[Ref.: #43487]	N-acetyl-beta-glucosaminidase	-	3.2.1.52
[Ref.: #43487]	naphthol-AS-BI-phosphohydrolase	+
[Ref.: #43487]	trypsin	-	3.4.21.4
[Ref.: #43487]	valine arylamidase	+
	Only first 10 entries are displayed. Click here to see all.

Fatty acid profile

Metadata FA analysis

Reference

[Ref.: #43487]

Fatty acid	Percentage	ECL
C15:1 iso G	26.6
C15:0 iso	20
Unknown 13.565	12	13565
C16:1ω7c /C16:1ω6c	10.6
C15:0 iso 3OH	5.5
Unknown 11.543	5.1	11543
C15:0	4.2
C17:0 iso 3OH	4.2
C14:0 iso	2.6
C16:0 iso 3OH	2.6
C15:0 anteiso	2
C16:0 iso	1.7
C15:1 anteiso A	1.4

Information on isolation source, the sampling and environmental conditions Isolation, sampling and environmental information

[Ref.: #43487]	Sample type/isolated from	brown alga, Sargassum fulvellum, collected from a natural seaweed bed in the South Sea
[Ref.: #43487]	Geographic location (country and/or sea, region)	South Korea
[Ref.: #43487]	Country	Republic of Korea
[Ref.: #43487]	Country ISO 3 Code	KOR
[Ref.: #43487]	Continent	Asia
[Ref.: #43487]	Geographic location	33.4970°/126.9160°

[Ref.: #67770]	Sample type/isolated from	Brown alga collected from the South Sea
[Ref.: #67770]	Country	Republic of Korea
[Ref.: #67770]	Country ISO 3 Code	KOR
[Ref.: #67770]	Continent	Asia

[Ref.: #67771]	Sample type/isolated from	From gulfweed
[Ref.: #67771]	Geographic location (country and/or sea, region)	Jeju Island
[Ref.: #67771]	Country	Republic of Korea
[Ref.: #67771]	Country ISO 3 Code	KOR
[Ref.: #67771]	Continent	Asia
* marker position based on {}

Isolation sources categories

#Environmental	#Aquatic	#Marine
#Host	#Algae	#Brown Algae

What are isolation sources categories?

Information on genomic background e.g. entries in nucleic sequence databass Sequence information

16S Sequence information:

	Sequence accession description	Seq. accession number	Sequence length (bp)	Sequence database	Associated NCBI tax ID
[Ref.: #43487]	16S rRNA gene sequence	KX279346		GenBank

Genome sequence information:

	Sequence accession description	Seq. accession number	Assembly level	Sequence database	Associated NCBI tax ID
[Ref.: #67770]	Winogradskyella flava KCTC 52348	GCA_014243395	contig	GenBank	1884876 tax ID
[Ref.: #66792]	Winogradskyella flava strain KCTC 52348	1884876.3	wgs	PATRIC	1884876 tax ID	*
[Ref.: #66792]	Winogradskyella flava KCTC 52348	2915080438	draft	JGI IMG/M	1884876 tax ID	*

[Ref.: #67770]

GC-content

36 mol%

thermal denaturation, midpoint method (Tm)

Data predicted using genome information as a basis Genome-based predictions

	Trait	Prediction	Confidence in %	In training data
	Predictions from GenomeNet Sporulation v. 1 based on: Winogradskyella flava strain KCTC 52348 PATRIC: 1884876.3
[Ref.: #69481]	spore-forming	no	100	no

	Trait	Prediction	Confidence in %	In training data
	Predictions from Diaspora project based on: Winogradskyella flava strain KCTC 52348 PATRIC: 1884876.3
[Ref.: #69480]	motile	no	85.979	no
[Ref.: #69480]	gram-positive	no	98.413	yes
[Ref.: #69480]	anaerobic	no	99.23	yes
[Ref.: #69480]	aerobic	yes	89.99	yes
[Ref.: #69480]	halophile	no	73.424	no
[Ref.: #69480]	spore-forming	no	97.013	no
[Ref.: #69480]	glucose-util	yes	79.919	yes
[Ref.: #69480]	flagellated	no	95.831	yes
[Ref.: #69480]	thermophile	no	99.083	no
[Ref.: #69480]	glucose-ferment	no	90.163	no

Availability in culture collections External links

[Ref.: #43487]

Culture collection no.

KCTC 52348, JCM 31798

Literature:

Topic	Title	Authors	Journal	DOI	Year
Phylogeny	Winogradskyella ouciana sp. nov., isolated from the hadal seawater of the Mariana Trench.	He X, Liu R, Liang J, Li Y, Zhao X, Ran L, Ahmad W, Zhang XH	Int J Syst Evol Microbiol	10.1099/ijsem.0.004687	2021	*
Phylogeny	Winogradskyella flava sp. nov., isolated from the brown alga, Sargassum fulvellum.	Lee JH, Kang JW, Shin SB, Seong CN	Int J Syst Evol Microbiol	10.1099/ijsem.0.002161	2017	*

References

#20215

Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )

#43487

Ji Hee Lee, Joo Won Kang, Soon Bum Shin, Chi Nam Seong: Winogradskyella flava sp. nov., isolated from the brown alga, Sargassum fulvellum. IJSEM 67: 3540 - 3546 2017 ( DOI 10.1099/ijsem.0.002161 , PubMed 28866993 )

#66792

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Automatically annotated for the DiASPora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#67770 Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#67771 Korean Collection for Type Cultures (KCTC) ; Curators of the KCTC;

#69480

Julia Koblitz, Joaquim Sardà, Lorenz Christian Reimer, Boyke Bunk, Jörg Overmann: Predictions based on genome sequence made in the Diaspora project (Digital Approaches for the Synthesis of Poorly Accessible Biodiversity Information) .

#69481

Xiao-Yin To, René Mreches, Martin Binder, Alice C. McHardy, Philipp C. Münch: Predictions based on the model GenomeNet Sporulation v. 1 . ( DOI 10.21203/rs.3.rs-2527258/v1 )

* These data were automatically processed and therefore are not curated

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Winogradskyella flava strain KCTC 52348

Winogradskyella flava strain KCTC 52348

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