Amphibacillus jilinensis (DSM 24783, CGMCC 1.5123, JCM 16149)

Name: Amphibacillus jilinensis (DSM 24783, CGMCC 1.5123, JCM 16149)
Creator: BacDive
License: http://creativecommons.org/licenses/by/4.0/

BacDive ID 1407

Type strain

Strain Designation Y1

Culture col. no. DSM 24783 CGMCC 1.5123 JCM 16149 Y1

NCBI tax ID(s) 1216008

Special Collections DSMZ JCM Literature strains

Links

Amphibacillus jilinensis Y1 is a facultative anaerobe, halotolerant, spore-forming prokaryote that was isolated from sediment from soda lake.

spore-forming Gram-positive motile rod-shaped facultative anaerobe halotolerant mesophilic genome sequence 16S sequence

Name and taxonomic classification

SI-ID 406670

JCM 16149,DSM 24783

@ref 20215

Last LPSN update 2025-12-16 09:47:06

Domain Bacillati

Phylum Bacillota

Class Bacilli

Order Caryophanales

Family Bacillaceae

Genus Amphibacillus

Species Amphibacillus jilinensis

Full scientific name Amphibacillus jilinensis Wu et al. 2010

Morphology

Cell morphology

@ref	Gram stain	Cell length	Cell width	Cell shape	Motility	Flagellum arrangement
23033	positive	2.0-3.2 µm	0.4-0.6 µm	rod-shaped		peritrichous

Culture and growth conditions

Culture medium

@ref	Name	Growth	Medium link	Composition
17771	CASO AGAR (MERCK 105458) (DSMZ Medium 220)		Medium recipe at MediaDive	Name: CASO AGAR (Merck 105458) (DSMZ Medium 220) Composition: Agar 15.0 g/l Casein peptone 15.0 g/l NaCl 5.0 g/l Soy peptone 5.0 g/l Distilled water
23033	optimized medium JY			containing l-1 distilled water: 2.0 g yeast extract (Difco), 5.0 g sucrose, 0.2 g KCl, 0.2 g KH2PO4 , 0.1 g MgCl2.6H2O, 0.5 g NH4Cl, 0.1 g CaCl2 , 0.06 M NaHCO3 and 0.44 M NaCl; final pH 9.0

Culture temp

@ref	Growth	Type	Temperature (°C)	Range
17771	positive	growth	30	mesophilic
23033	positive	growth	15.0-45.0
23033	negative	growth	10.0
23033	negative	growth	50.0
23033	positive	optimum	32.0	mesophilic
67770	positive	growth	35	mesophilic

Culture pH

@ref	Ability	Type	PH	PH range
23033	positive	optimum	9.0	alkaliphile
23033	positive	growth	7.5-10.5
23033		growth	7.0
23033		growth	11.0

Physiology and metabolism

Oxygen tolerance

@ref	Oxygen tolerance	Confidence
23033	facultative anaerobe
125439	obligate aerobe	95.7

Spore formation

@ref	Spore description	Type of spore	Spore formation
23033	terminal	endospore

Halophily

23033

Halophily levelhalotolerant

Metabolite utilization

@ref	Chebi-ID	Metabolite	Utilization activity	Kind of utilization tested
23033	30089 ChEBI	acetate	-	growth
23033	17968 ChEBI	butyrate	-	growth
23033		casamino acids	-	growth
23033		casein	+	hydrolysis
23033	17057 ChEBI	cellobiose	+	carbon source
23033	16947 ChEBI	citrate	-	growth
23033	15824 ChEBI	D-fructose	+	carbon source
23033	12936 ChEBI	D-galactose	+	carbon source
23033	17634 ChEBI	D-glucose	+	carbon source
23033	16899 ChEBI	D-mannitol	+	carbon source
23033	17924 ChEBI	D-sorbitol	+	carbon source
23033	65327 ChEBI	D-xylose	+	carbon source
23033	16236 ChEBI	ethanol	-	growth
23033	15740 ChEBI	formate	-	growth
23033	29806 ChEBI	fumarate	-	growth
23033	17234 ChEBI	glucose	-	fermentation
23033	17754 ChEBI	glycerol	-	growth
23033	15428 ChEBI	glycine	-	growth
23033	17368 ChEBI	hypoxanthine	-	hydrolysis
23033	16977 ChEBI	L-alanine	-	growth
23033	30849 ChEBI	L-arabinose	+	carbon source
23033	16467 ChEBI	L-arginine	-	growth
23033	17561 ChEBI	L-cysteine	-	growth
23033	15971 ChEBI	L-histidine	-	growth
23033	16643 ChEBI	L-methionine	-	growth
23033	24996 ChEBI	lactate	-	growth
23033	17716 ChEBI	lactose	+	carbon source
23033	25115 ChEBI	malate	-	growth
23033	17306 ChEBI	maltose	+	carbon source
23033	37684 ChEBI	mannose	+	carbon source
23033	28053 ChEBI	melibiose	+	carbon source
23033	17790 ChEBI	methanol	-	growth
23033	30623 ChEBI	oxalate	-	growth
23033	17272 ChEBI	propionate	-	growth
23033	15361 ChEBI	pyruvate	-	growth
23033	16634 ChEBI	raffinose	+	carbon source
23033	26546 ChEBI	rhamnose	+	carbon source
23033	28017 ChEBI	starch	+	hydrolysis
23033	30031 ChEBI	succinate	-	growth
23033	17992 ChEBI	sucrose	+	carbon source
23033	30929 ChEBI	tartrate	-	growth
23033	27082 ChEBI	trehalose	+	carbon source
23033		tryptone	-	growth
23033	18186 ChEBI	tyrosine	-	hydrolysis
23033	15318 ChEBI	xanthine	-	hydrolysis
23033		yeast extract	+	growth

Antibiotic resistance

@ref	ChEBI	Metabolite	Resistance conc.	Sensitivity conc.
23033	2637	amikacin	30 µg (disc)
23033	2676	amoxicillin		10 µg (disc)
23033	28971	ampicillin		10 µg (disc)
23033	2955	azithromycin		15 µg (disc)
23033	28669	bacitracin	0.04 Unit
23033	3393	carbenicillin		100 µg (disc)
23033	204928	cefotaxime		30 µg (disc)
23033	209807	cefoxitin		300 µg (disc)
23033	17698	chloramphenicol		30 µg (disc)
23033	48923	erythromycin		15 µg (disc)
23033	28915	fosfomycin		200 µg (disc)
23033	17833	gentamicin	10 µg (disc)
23033	6104	kanamycin		30 µg (disc)
23033	100147	nalidixic acid	30 µg (disc)
23033	7507	neomycin		30 µg (disc)
23033	71415	nitrofurantoin		300 µg (disc)
23033	28368	novobiocin		30 µg (disc)
23033	7660	nystatin	100 µg (disc)
23033	7809	oxacillin		1 µg (disc)
23033	17334	penicillin		10 µg (disc)
23033	8309	polymyxin b	300 Unit
23033	28077	rifampicin		5 µg (disc)
23033	17076	streptomycin		10 µg (disc)
23033	27902	tetracycline		30 µg (disc)
23033	28864	tobramycin		10 µg (disc)
23033	28001	vancomycin		30 µg (disc)

Metabolite production

@ref	Chebi-ID	Metabolite
23033	15688 ChEBI	acetoin
23033	16136 ChEBI	hydrogen sulfide
23033	35581 ChEBI	indole

Metabolite tests

@ref	Chebi-ID	Metabolite	Voges-proskauer-test	Methylred-test
23033	15688 ChEBI	acetoin	-
23033	17234 ChEBI	glucose		-

Enzymes

@ref	Value	Activity	Ec
23033	catalase	-	1.11.1.6
23033	cytochrome oxidase	-	1.9.3.1

Isolation, sampling and environmental information

Isolation source categories

Cat1	Cat2	Cat3
#Environmental	#Aquatic	#Sediment
#Environmental	#Aquatic	#Lake (large)
#Condition	#Alkaline	-

Isolation

@ref	Sample type	Geographic location	Country	Country ISO 3 Code	Continent	Latitude	Longitude	Enrichment culture	Enrichment culture composition	Enrichment culture duration	Enrichment culture temperature	Isolation procedure
17771	sediment from soda lake	Jilin Province	China	CHN	Asia
23033	soda lake	Jilin Province	China	CHN	Asia	44.75	123.567 44.75/123.567	enrichment culture	containing (l-1 distilled water) 10.0 g NaCl, 10.0 g Na2CO3, 5.0 g NaHCO3 , 0.2 g KH2PO4, 0.33 g MgCl2.6H2O, 0.5 g NH4Cl, 0.2 g KCl, 2.0 g yeast extract (Difco), 5.0 g glucose, 0.5 g Na2S.9H2O, 0.4 g L-cysteine, 10 ml trace element solution 141 and 0.001 g resazurin	3 days	32	Hungate roll-tube technique
67770	Sediment from a soda lake in Jilin Province		China	CHN	Asia

Taxonmaps

69479

Global distribution of 16S sequence FJ169626 (>99% sequence identity) for Amphibacillus jilinensis from Microbeatlas

Aquatic Samples	41
Soil Samples	96
Animal Samples	131
Plant Samples	22
Total Samples	290

Interaction and safety

Risk assessment

@ref	Biosafety level	Biosafety level comment
17771	1	Risk group (German classification) According to TRBA 466

Sequence information

Genome sequences

@ref	Description	Assembly level	INSDC accession	BV-BRC accession	IMG accession	NCBI tax ID	Score
67770	ASM30696v1 assembly for Amphibacillus jilinensis Y1	scaffold	GCA_000306965	562743.4	2547132169	562743	56.93

16S sequences

@ref	Description	Accession	Length	Database	NCBI tax ID
17771	Amphibacillus jilinensis Y1 16S ribosomal RNA gene, partial sequence	FJ169626	1532		562743

GC content

@ref	GC-content (mol%)	Method
17771	37.7	thermal denaturation, midpoint method (Tm)
67770	37.27	genome sequence analysis

Genome-based predictions

Predictions

Predictions from Deep Learning for Genome Sequence Data. R package version 0.3.1 based on: Amphibacillus jilinensis Y1
NCBI: GCA_000306965

@ref	Trait	Model	Prediction	Confidence in %	In training data
125439	spore_formation	BacteriaNetⓘ	yes	89.50	no
125439	motility	BacteriaNetⓘ	yes	81.10	no
125439	gram_stain	BacteriaNetⓘ	variable	78.80	no
125439	oxygen_tolerance	BacteriaNetⓘ	obligate aerobe	95.70	no

Predictions from bacterial phenotypic traits through improved machine learning using high-quality, curated datasets based on: Amphibacillus jilinensis Y1
PATRIC: 562743.4

@ref	Trait	Model	Prediction	Confidence in %	In training data
125438	gram-positive	gram-positiveⓘ	yes	83.61	yes
125438	anaerobic	anaerobicⓘ	no	82.29	no
125438	spore-forming	spore-formingⓘ	yes	87.04	yes
125438	aerobic	aerobicⓘ	no	85.41	no
125438	thermophilic	thermophileⓘ	no	92.14	yes
125438	flagellated	motile2+ⓘ	yes	83.41	no

Literature

Topic	Title	Authors	Journal	DOI	Year
	Draft Genome Sequence of Amphibacillus jilinensis Y1(T), a Facultatively Anaerobic, Alkaliphilic and Halotolerant Bacterium.	Cheng H, Fang MX, Jiang XW, Wu M, Zhu XF, Zheng G, Yang ZJ.	Stand Genomic Sci	10.4056/sigs.4107829	2013
Genetics	Genome-wide survey and phylogeny of S-Ribosylhomocysteinase (LuxS) enzyme in bacterial genomes.	Rao RM, Pasha SN, Sowdhamini R.	BMC Genomics	10.1186/s12864-016-3002-x	2016
Enzymology	No evidence for enzootic plague within black-tailed prairie dog (Cynomys ludovicianus) populations.	Colman RE, Brinkerhoff RJ, Busch JD, Ray C, Doyle A, Sahl JW, Keim P, Collinge SK, Wagner DM.	Integr Zool	10.1111/1749-4877.12546	2021
Enzymology	A Novel Multi-domain High Molecular, Salt-Stable Alkaline Xylanase from Alkalibacterium sp. SL3.	Wang G, Wu J, Yan R, Lin J, Ye X.	Front Microbiol	10.3389/fmicb.2016.02120	2016
Phylogeny	Amphibacillus marinus sp. nov., a member of the genus Amphibacillus isolated from marine mud.	Ren B, Yang N, Wang J, Ma XL, Wang Q, Xie F, Guo H, Liu ZH, Pugin B, Zhang LX.	Int J Syst Evol Microbiol	10.1099/ijs.0.045807-0	2013
Phylogeny	Amphibacillus cookii sp. nov., a facultatively aerobic, spore-forming, moderately halophilic, alkalithermotolerant bacterium.	Pugin B, Blamey JM, Baxter BK, Wiegel J.	Int J Syst Evol Microbiol	10.1099/ijs.0.034629-0	2012
Phylogeny	Amphibacillus jilinensis sp. nov., a facultatively anaerobic, alkaliphilic bacillus from a soda lake.	Wu XY, Zheng G, Zhang WW, Xu XW, Wu M, Zhu XF	Int J Syst Evol Microbiol	10.1099/ijs.0.018259-0	2009

References

#17771	Leibniz Institut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH ; Curators of the DSMZ; DSM 24783
#20215	Parte, A.C., Sardà Carbasse, J., Meier-Kolthoff, J.P., Reimer, L.C. and Göker, M.: List of Prokaryotic names with Standing in Nomenclature (LPSN) moves to the DSMZ. IJSEM ( DOI 10.1099/ijsem.0.004332 )
#23033	Xiao-Yue Wu,Gang Zheng,Wen-Wu Zhang,Xue-Wei Xu,Min Wu,Xu-Fen Zhu: Amphibacillus jilinensis sp. nov., a facultatively anaerobic, alkaliphilic bacillus from a soda lake. IJSEM 60: 2540 - 2543 2010 ( DOI 10.1099/ijs.0.018259-0 , PubMed 19965990 )
#67770	Japan Collection of Microorganism (JCM) ; Curators of the JCM;
#69479	João F Matias Rodrigues, Janko Tackmann,Gregor Rot, Thomas SB Schmidt, Lukas Malfertheiner, Mihai Danaila,Marija Dmitrijeva, Daniela Gaio, Nicolas Näpflin and Christian von Mering. University of Zurich.: MicrobeAtlas 1.0 beta .
#125438	Julia Koblitz, Lorenz Christian Reimer, Rüdiger Pukall, Jörg Overmann: Predicting bacterial phenotypic traits through improved machine learning using high-quality, curated datasets. 2024 ( DOI 10.1101/2024.08.12.607695 )
#125439	Philipp Münch, René Mreches, Martin Binder, Hüseyin Anil Gündüz, Xiao-Yin To, Alice McHardy: deepG: Deep Learning for Genome Sequence Data. R package version 0.3.1 .
#126262	A. Lissin, I. Schober, J. F. Witte, H. Lüken, A. Podstawka, J. Koblitz, B. Bunk, P. Dawyndt, P. Vandamme, P. de Vos, J. Overmann, L. C. Reimer: StrainInfo—the central database for linked microbial strain identifiers. ( DOI 10.1093/database/baaf059 )

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Amphibacillus jilinensis (DSM 24783, CGMCC 1.5123, JCM 16149)

Name and taxonomic classification

Morphology

Cell morphology

Culture and growth conditions

Culture medium

Culture temp

Culture pH

Physiology and metabolism

Oxygen tolerance

Spore formation

Halophily

Metabolite utilization

Antibiotic resistance

Metabolite production

Metabolite tests

Enzymes

Isolation, sampling and environmental information

Isolation source categories

Isolation

Taxonmaps

Interaction and safety

Risk assessment

Sequence information

Genome sequences

Genome browser: GCA_000306965

16S sequences

GC content

Genome-based predictions

Predictions

Literature

Literature

References

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